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Gene information
General information
TC012451 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7700446..7708152
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012451
    iBeetle screen iB_05244

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012451 RA
      MSLTTENIK IISFDVKDI RFPTSLEAH GSDAMHTDP 
      DYSCAYVTI KLKTGLEGH GLTFTCGRG TEIVVAACK 
      SLSDLIVGQ VVSDIYKDF GGFWRSLTS ESQLRWVGP 
      EKGVIHLAT AAIINALWD LWGKLLNKP VWQLLADLE 
      PEQLVSTID FRYLSNVIT KEEAVELLR RGRVGKEER 
      MNQLLALGY PAYTTQAGW LGYSDETIY DLANKYMEQ 
      GFNAFKVKV GKNLFQDIK RLELIRNTI GWDRILMVD 
      ANQIWEVQE AIDWMKQLS HFKILWIEE PTSPDDVLG 
      HAEIAKALK PLGIGVATG EMCCNRVLF KQFLQANAL 
      EYCQIDSAR IGGVNEILS VYLMCKKLG VKVCPHAGG 
      VGLCEMVQH LQMWDYVAL SGTMDGRFI EYVDQQHDQ 
      FVNPTIVEN ACYMAPRTP GYSTTLKPE AISAYENIP
    CDS sequence
    • >TC012451 RA
      atgagtcta acaaccgaa aatataaaa ataatctcc 
      ttcgatgtg aaagacatc cgcttcccc acctccctg 
      gaagcgcac ggttcggat gctatgcac actgaccct 
      gactactcc tgcgcctac gtcaccatc aaactcaag 
      acaggcctg gagggccac ggcctgacc ttcacctgc 
      ggcagagga acggaaatc gtcgttgca gcgtgcaag 
      tcgctgagt gacctcatc gttggtcaa gtggtcagc 
      gatatttac aaggatttt ggcggcttc tggcgcagc 
      ttgaccagc gagagccag ctgaggtgg gtcgggccg 
      gagaaaggg gtaatacac ctcgccaca gccgccatt 
      attaacgcc ctgtgggac ctgtggggg aaactgctc 
      aataagcca gtgtggcaa cttttggcc gatttggag 
      cccgaacag ttggtctca actatagat ttccggtac 
      ttgtcaaat gttataacg aaagaagag gccgtcgaa 
      ttgctgcga agggggcga gtggggaag gaggaaagg 
      atgaaccag ctgttggct ctgggatac cccgcctac 
      accacacaa gccggctgg ttggggtac agcgacgag 
      acgatctac gacctggcc aacaaatac atggagcaa 
      ggcttcaac gcatttaaa gtcaaagtt gggaaaaac 
      ctattccag gacataaag cgcctcgag ttgattcgc 
      aacaccatc ggctgggac cgcattttg atggtggac 
      gcgaaccaa atctgggaa gtccaggaa gctatcgat 
      tggatgaaa caactatca cattttaaa attttatgg 
      attgaggag cccacgtcc cctgacgat gtgctaggc 
      catgccgaa atcgccaaa gccttgaaa ccgctcggc 
      atcggcgtt gcaacgggc gaaatgtgc tgcaaccga 
      gtgctcttc aagcaattc ctgcaagcc aacgctttg 
      gagtattgc caaattgac tcggcacga atcggcggc 
      gttaacgag attttaagc gtgtacttg atgtgcaaa 
      aaactggga gttaaagtt tgtccccat gctggaggg 
      gtcggatta tgcgaaatg gtccaacac ttgcaaatg 
      tgggactat gtggccttg tcgggcacc atggatggg 
      cggtttatt gaatacgtt gaccaacaa cacgaccaa 
      tttgtcaac ccaactatt gtcgaaaac gcctgctac 
      atggccccc aggactcct ggctacagc accaccctg 
      aagcctgag gcaataagc gcgtacgag aatattccg 
      tga
    mRNA sequence
    • >TC012451 RA
      cattgcttt tatttgttt tttctatct tctggtaac 
      atctataaa accatcaaa aatatgtca ctgcatctg 
      atgtgctgt cgcgaccgt aatttttct tgcatcaca 
      aatttaaac acataggat aaacaagaa tcaagataa 
      caccttatc taaacaatg caaaagcgg cctcgtgat 
      ccaccgaca taaattccc aaaacagct caaaaatcg 
      cagtgccgt cacctcgtc cgcaccatg agtctaaca 
      accgaaaat ataaaaata atctccttc gatgtgaaa 
      gacatccgc ttccccacc tccctggaa gcgcacggt 
      tcggatgct atgcacact gaccctgac tactcctgc 
      gcctacgtc accatcaaa ctcaagaca ggcctggag 
      ggccacggc ctgaccttc acctgcggc agaggaacg 
      gaaatcgtc gttgcagcg tgcaagtcg ctgagtgac 
      ctcatcgtt ggtcaagtg gtcagcgat atttacaag 
      gattttggc ggcttctgg cgcagcttg accagcgag 
      agccagctg aggtgggtc gggccggag aaaggggta 
      atacacctc gccacagcc gccattatt aacgccctg 
      tgggacctg tgggggaaa ctgctcaat aagccagtg 
      tggcaactt ttggccgat ttggagccc gaacagttg 
      gtctcaact atagatttc cggtacttg tcaaatgtt 
      ataacgaaa gaagaggcc gtcgaattg ctgcgaagg 
      gggcgagtg gggaaggag gaaaggatg aaccagctg 
      ttggctctg ggatacccc gcctacacc acacaagcc 
      ggctggttg gggtacagc gacgagacg atctacgac 
      ctggccaac aaatacatg gagcaaggc ttcaacgca 
      tttaaagtc aaagttggg aaaaaccta ttccaggac 
      ataaagcgc ctcgagttg attcgcaac accatcggc 
      tgggaccgc attttgatg gtggacgcg aaccaaatc 
      tgggaagtc caggaagct atcgattgg atgaaacaa 
      ctatcacat tttaaaatt ttatggatt gaggagccc 
      acgtcccct gacgatgtg ctaggccat gccgaaatc 
      gccaaagcc ttgaaaccg ctcggcatc ggcgttgca 
      acgggcgaa atgtgctgc aaccgagtg ctcttcaag 
      caattcctg caagccaac gctttggag tattgccaa 
      attgactcg gcacgaatc ggcggcgtt aacgagatt 
      ttaagcgtg tacttgatg tgcaaaaaa ctgggagtt 
      aaagtttgt ccccatgct ggaggggtc ggattatgc 
      gaaatggtc caacacttg caaatgtgg gactatgtg 
      gccttgtcg ggcaccatg gatgggcgg tttattgaa 
      tacgttgac caacaacac gaccaattt gtcaaccca 
      actattgtc gaaaacgcc tgctacatg gcccccagg 
      actcctggc tacagcacc accctgaag cctgaggca 
      ataagcgcg tacgagaat attccgtga tccacgaaa 
      acgtaaata aatatattg tttgtac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY