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Gene information
General information
TC012432 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7316443..7319441
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012432
    iBeetle screen iB_05242

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012432 RA
      MGFVIELGI ISLSLLFIA TFIIKNKNP PAILGIYQR 
      PNGFFYFKF IFMFTILSL KKLGHKFRK RKQMEEITA 
      LDRPQKLSA HPKAIDAVY FNGANKHGD YLVVGTARR 
      KNKLVDGFL YLLVESSGF GLLETPKLP GTALYQSEE 
      SEEFAAEGL RIEPQVPMG KWRISYEGK MKRFENRKE 
      FVDVKIEAE WSSTLPYFN FDSDMDPWA MATSMAYET 
      FSREYFQNL KANHQTHYE QHGDIKGVA IINGRSFSI 
      EINAVRDHS FGLQREWRN FHRYGLHFV TAENGDRFN 
      VGKICLPIS FSRLTIGYV YSAEDGKIH PVRSCDLEL 
      HQHGEGGDP PEDYAFSFE TGKKRYVMQ VNVVHSPHF 
      YISKDWESK VVERLCTFE INGMKGWGA AEWQYRNVR 
      EKKLKMKLL LIVLFAIFC GSSLQLEEK ENFRDSHIS 
      FKRSSTTNG CSELQIMQC KFRKCILGA YCVNGVCKC 
      NIPIK
    CDS sequence
    • >TC012432 RA
      atgggtttt gtgatcgaa cttggtatt atttcccta 
      tccttacta tttatagcc acattcata attaaaaac 
      aaaaacccc cccgctatt ttgggcata taccagcga 
      ccgaacggc ttcttttac tttaaattc atcttcatg 
      tttacaatt ctgtcgtta aaaaagtta gggcacaaa 
      tttcggaaa cgcaaacaa atggaggaa ataaccgca 
      cttgacagg ccccaaaaa ttatcagcg catccaaag 
      gctattgac gctgtgtac ttcaacggg gccaacaaa 
      cacggtgat tatttagtc gttgggaca gcccgtcgg 
      aaaaacaaa ctagtcgat gggttcctc tacctgttg 
      gtagaaagt tcggggttt ggtttattg gagactccg 
      aaattgccc ggaactgct ctttaccaa agcgaggag 
      agcgaggag tttgcagct gaggggctc agaatagag 
      ccccaggtg ccgatgggc aagtggagg atttcgtac 
      gaaggcaaa atgaaacgg ttcgaaaac cggaaggag 
      tttgttgat gtgaaaatc gaagctgag tggagttcc 
      actctgccg tattttaac tttgatagt gatatggat 
      ccctgggcg atggccacg tccatggct tacgagacg 
      ttcagtcgg gagtatttc caaaatttg aaggcgaac 
      caccaaact cattacgaa caacacggt gacattaaa 
      ggcgtcgcg attataaac gggcgttcc ttcagcatt 
      gaaattaac gccgtgagg gaccacagc ttcggcttg 
      caaagagaa tggaggaac tttcatcgg tatggtcta 
      cactttgtc actgcggaa aacggcgat aggttcaat 
      gttgggaag atttgtttg ccgatctcc ttttcaaga 
      ttaacaatt ggatacgtc tattcagcc gaggacggg 
      aaaatacac ccggtacgg tcctgcgac ttggaactg 
      caccagcac ggtgaaggc ggggatcca ccggaggat 
      tatgcgttc agttttgaa acaggaaaa aaacgatat 
      gtgatgcaa gtcaacgtt gtgcactct ccgcatttt 
      tatattagt aaagactgg gaaagtaaa gttgtggaa 
      aggttgtgc acttttgaa attaatggg atgaaaggg 
      tggggtgct gcggaatgg cagtataga aacgttcgt 
      gagaaaaaa ttaaaaatg aagttgtta ctgatcgtc 
      ttatttgca attttttgt ggtagttca cttcaactg 
      gaagagaaa gaaaatttt agagattct catatcagt 
      ttcaaaaga tcgtctaca actaatggg tgttcagaa 
      ttacaaata atgcagtgt aagtttaga aaatgtatt 
      ctgggcgct tattgtgtt aatggagtt tgtaaatgt 
      aatataccc ataaaataa
    mRNA sequence
    • >TC012432 RA
      gtcgcctcg aaaaagcaa aaatgggtt ttgtgatcg 
      aacttggta ttatttccc tatccttac tatttatag 
      ccacattca taattaaaa acaaaaacc cccccgcta 
      ttttgggca tataccagc gaccgaacg gcttctttt 
      actttaaat tcatcttca tgtttacaa ttctgtcgt 
      taaaaaagt tagggcaca aatttcgga aacgcaaac 
      aaatggagg aaataaccg cacttgaca ggccccaaa 
      aattatcag cgcatccaa aggctattg acgctgtgt 
      acttcaacg gggccaaca aacacggtg attatttag 
      tcgttggga cagcccgtc ggaaaaaca aactagtcg 
      atgggttcc tctacctgt tggtagaaa gttcggggt 
      ttggtttat tggagactc cgaaattgc ccggaactg 
      ctctttacc aaagcgagg agagcgagg agtttgcag 
      ctgaggggc tcagaatag agccccagg tgccgatgg 
      gcaagtgga ggatttcgt acgaaggca aaatgaaac 
      ggttcgaaa accggaagg agtttgttg atgtgaaaa 
      tcgaagctg agtggagtt ccactctgc cgtatttta 
      actttgata gtgatatgg atccctggg cgatggcca 
      cgtccatgg cttacgaga cgttcagtc gggagtatt 
      tccaaaatt tgaaggcga accaccaaa ctcattacg 
      aacaacacg gtgacatta aaggcgtcg cgattataa 
      acgggcgtt ccttcagca ttgaaatta acgccgtga 
      gggaccaca gcttcggct tgcaaagag aatggagga 
      actttcatc ggtatggtc tacactttg tcactgcgg 
      aaaacggcg ataggttca atgttggga agatttgtt 
      tgccgatct ccttttcaa gattaacaa ttggatacg 
      tctattcag ccgaggacg ggaaaatac acccggtac 
      ggtcctgcg acttggaac tgcaccagc acggtgaag 
      gcggggatc caccggagg attatgcgt tcagttttg 
      aaacaggaa aaaaacgat atgtgatgc aagtcaacg 
      ttgtgcact ctccgcatt tttatatta gtaaagact 
      gggaaagta aagttgtgg aaaggttgt gcacttttg 
      aaattaatg ggatgaaag ggtggggtg ctgcggaat 
      ggcagtata gaaacgttc gtgagaaaa aattaaaaa 
      tgaagttgt tactgatcg tcttatttg caatttttt 
      gtggtagtt cacttcaac tggaagaga aagaaaatt 
      ttagagatt ctcatatca gtttcaaaa gatcgtcta 
      caactaatg ggtgttcag aattacaaa taatgcagt 
      gtaagttta gaaaatgta ttctgggcg cttattgtg 
      ttaatggag tttgtaaat gtaatatac ccataaaat 
      aaaagtgct ttcgtaact ctacttctt ttataaata 
      aatatgaca attacaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY