Status
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Gene information
General information
TC034331
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012427,TC012428
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7229640..7237828
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034331
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05241

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034331 RA
      MLRKIPKHV TIVLNETET LFLLELPSS VVPRDTDEG 
      EAAEMDNRL YEYLTKGKG RNRKVATTG VQTRPVLMK 
      TRNTEPVQY KFRDNSTFA SNWEMYDTY NASFVESVE 
      LEETNDHEL IAEVSLDEL RKSRETKEL EKLSVNKNY 
      FDAVLVIER LLANNCYNN EQKKFKGLV DEAEEIRYD 
      YKLDLLWTF ANEDTKNRC VTGISFNSF TEDMVAVGY 
      GKYYFIDRR KTGVVMVWN IKNPKQPER EYYFRHPVT 
      SLAFSTKDP NYLAVGFYN GELLVIDVS KRDFEMVGE 
      DSSTSFKPA WNMVWLEQN QKEFLLVCF GDGRVCKYL 
      VTQAKLECV QIMRTDKIE GKLKGLPAL RLFEGNQVS 
      SSRYAGALC LSVHKPDPA FYLVGTEEG VIHKCAASY 
      CTHHVDMFK AHEGPIHTI KFSPFVEKV FATCGDDFQ 
      KCLWIEGIS EPLIVSRFC MEPVLDVDW SPSHSTIIA 
      SLRGMDIFL WDLQRKIYL PQSQTKSPT NSRNTVCKF 
      SACGKCLVV GDIDGNVHT FAVSGLPIP AFFQQNLFF 
      QSIKNILQT NTDLIGKLE NWQIDNPCQ NLMASSPRT 
      GRSVTKSSV SYDPGYQSS LRKLRGKDR ESLRPKTIL 
      TAPKYQVIP VIFDGVDVT PISLNPYVF EGGRERQIS 
      IMDNLTKDS FMLHHHSLS RVESQKFQT NILLQKQEG 
      ETATFTHRS MVTSAYFGD EILLPDVSL RSKSEFSQQ 
      EIVYDEESV EGKPELPIP EHVSLVLVE TETMFLLDL 
      PSSTVSKTS EGANDVEED NKLYEFLTK GKGRNRKVI 
      AVGIQTVPI LKKARDTET KKMRFRNNS TYVSAWEMY 
      DTFQELEEE AHEEFEDKL SDEEMGTLV EEEHRLNDA 
      ASLESYRMS AEDKQLNKI VRSKAFHDA LIVIERILG 
      NNCYNAQQK RYKGLSEPD VFRENIEYK YRLELLWTY 
      ANDETDSRC VTGVAFNSF NEDLVAVAY GKYFYTDRS 
      ATGMVMIWN IKNPQQPER QYDFETAVT SLAFSKVNP 
      NLLAVGFYN GDLKILDVS KREISAIRE DDTFFEPVW 
      SILWFQQGG DEDLMVSFG DGRVCRYQI TTARDLKRT 
      QIMITAAAE GKLKGIKYL KKCEGKNIS ISGYDQARC 
      LAMHPCDPF TYFVGTSEG VVHRCSTNY YNEHMDLFK 
      AHDGAIHTI RFSPFANKL YYTCGDDFR ARLWVDGIS 
      ECLVTSEPS MLPVLDVDW SPEHPTVIA TIRGPDILI 
      WDLQRKMHQ SQSISKTPH NIFNTVCRF TPNGRSLMV 
      GDMKGNVHV YCLEDMPIP PFFPENLIS QSLKKLLVT 
      RPDLMKKLR KLGSLKFDD RQFSKYFN
    CDS sequence
    • >TC034331 RA
      atgctgcga aaaatccca aaacatgtg actattgtg 
      ttaaacgag acggaaacc cttttcttg cttgaactt 
      ccaagttct gtggtcccg cgggacact gacgagggc 
      gaagccgcc gagatggac aaccgcctt tacgaatac 
      ttaacaaag ggtaaaggg cgtaatcgc aaagttgca 
      acaactgga gtacaaacg aggcctgtt ttaatgaaa 
      acgcgaaac acggaaccc gtccagtac aagttcagg 
      gacaactca acgtttgct tcaaactgg gagatgtat 
      gatacctac aatgccagt tttgttgaa agtgttgag 
      ttggaggaa acaaacgac catgagtta attgcggaa 
      gtgtctttg gatgaacta aggaagagt cgagagacc 
      aaagagttg gagaagttg tcagtgaat aaaaactac 
      ttcgacgct gttctagta attgaacgc cttttggcc 
      aacaattgt tacaacaac gaacagaag aagttcaaa 
      gggttggtg gacgaggcc gaggaaatc cgctacgat 
      tacaagctg gacttgctg tggactttc gccaacgaa 
      gacacgaaa aataggtgc gttaccggg atctcgttc 
      aattctttc accgaagat atggtagct gtgggctat 
      ggcaagtac tattttatt gaccggaga aagaccggg 
      gttgtgatg gtgtggaac ataaaaaat cccaaacag 
      cccgagcgc gagtactac ttcagacac ccggtcacg 
      tcccttgcg ttttcaaca aaagacccg aactatctg 
      gctgttggg ttctacaat ggggagctc ctagttata 
      gatgtcagc aaaagggac tttgagatg gttggggag 
      gactctagc acttcgttt aaaccggcc tggaacatg 
      gtatggctg gagcaaaac cagaaggaa tttctcctg 
      gtttgtttc ggcgacggt cgggtttgc aagtacttg 
      gtgacccaa gcgaagctc gagtgtgta caaattatg 
      aggacggat aaaattgaa gggaagctg aaagggtta 
      ccagccttg cggttgttt gaaggcaat caggtttcg 
      tcgtctagg tatgccggc gctctttgc ttaagtgtg 
      cacaagccg gaccctgcc ttctacctt gtgggtaca 
      gaggaaggt gtcatacac aagtgtgcc gcaagttac 
      tgtacccac catgtggac atgttcaaa gcgcatgaa 
      ggccccatt cacactatt aagttttca ccatttgtt 
      gaaaaagtg tttgcgacc tgtggtgac gactttcag 
      aagtgtttg tggatcgag gggattagt gagcccttg 
      attgtctcg cggttttgc atggagccg gtgcttgat 
      gtcgactgg agtccgtca cactcgact atcattgca 
      agtcttcgc ggcatggac atcttcctg tgggacttg 
      caaaggaag atctacttg ccacagtcc caaactaag 
      tcacccacg aattcaaga aacacggtt tgtaagttt 
      agtgcttgt ggcaagtgt ttagtggtc ggggacatt 
      gacggtaat gtccacacg tttgctgtg agcgggcta 
      ccaattcct gccttcttt caacaaaac ctatttttc 
      cagtcgatt aaaaacatc ctgcaaacc aatacggat 
      ctaatcggc aaactcgaa aattggcaa attgacaat 
      ccctgtcaa aatctgatg gcttcttcg ccccgaacc 
      ggccgttcg gtcaccaaa tccagcgtt tcgtacgac 
      cctggctac caaagcagc ctccgcaaa ctgcgcggc 
      aaagaccgg gaatctctc cgccccaaa acaatcctc 
      accgccccc aaataccaa gtcatcccc gtgattttc 
      gatggcgtg gacgtcacc cccatatcc ctcaacccc 
      tacgtcttc gagggcggg cgcgagcgc cagatcagc 
      atcatggac aacctgacc aaagacagc ttcatgctg 
      caccaccac tcgctcagc cgagtcgaa tcccaaaag 
      ttccaaacc aacatcctg ctccagaag caggaaggc 
      gagactgcg accttcacg caccggagc atggtaact 
      tcggcctac ttcggcgac gaaatcctc ctcccggat 
      gtgagtttg aggagcaaa tcggagttt tcgcagcag 
      gagattgtg tacgatgag gagtcggtg gaagggaag 
      cctgaactg ccgatcccg gagcatgtt agtctggtc 
      ctggtggag accgagacc atgttcctg ctggatttg 
      ccgagttcg accgtttcg aagacaagt gaaggggcc 
      aacgatgtt gaggaggac aacaagttg tacgagttt 
      ctgaccaag gggaagggg cgcaatcgg aaggtgata 
      gcagtgggg atacaaact gtgccgatt ttgaagaaa 
      gcaagggac acggagacg aagaagatg aggtttagg 
      aacaattcc acgtacgtt tccgcctgg gagatgtac 
      gatacgttc caagagttg gaggaggaa gcccatgag 
      gagttcgaa gataagttg agtgatgaa gaaatgggg 
      actttggtt gaggaagag cacaggttg aacgacgct 
      gcgtccctt gagagttac cggatgtcc gccgaagac 
      aaacagttg aacaaaatt gtgaggagt aaagctttc 
      catgatgcc cttattgtc attgaacgc atccttggg 
      aataactgt tacaacgcg cagcaaaag cgctacaaa 
      ggcctttcg gagccggac gtcttccgc gaaaacatc 
      gaatacaag tacaggttg gaacttcta tggacttac 
      gcgaatgat gaaaccgac tccagatgt gtcaccgga 
      gtggccttc aattccttc aacgaagac ttagttgca 
      gtggcgtac ggcaagtac ttctacact gaccggagc 
      gcaaccgga atggtgatg atctggaac atcaaaaat 
      ccgcaacag ccggaacgc cagtacgac tttgaaacg 
      gccgtaaca tctcttgcg ttttctaaa gtgaacccg 
      aatttgctc gctgtgggg ttctacaac ggcgatttg 
      aaaattctg gacgttagt aagcgggaa atttcggcg 
      attcgggaa gacgatacg tttttcgaa cccgtctgg 
      agcatcctt tggttccag caaggcggg gatgaggac 
      cttatggtt agtttcggg gacggccgt gtgtgtcgc 
      taccaaata acgacagct cgggatttg aaacggacg 
      cagattatg ataactgcc gcggccgaa gggaagttg 
      aaagggatc aagtatttg aaaaaatgt gaagggaag 
      aatatctcc atttcggga tatgaccaa gccaggtgt 
      ttggcgatg cacccgtgt gaccccttc acttacttc 
      gtcgggacc agtgaaggg gtggttcat agatgctcc 
      actaactac tacaacgag cacatggac ctgttcaag 
      gcccatgat ggtgcaatc cacaccata cgtttctcc 
      ccttttgcc aacaagttg tactacact tgtggtgac 
      gattttagg gcgcgtctt tgggtagac ggtatttcg 
      gaatgtttg gtaacttcg gaaccgtcc atgttacca 
      gtgctggac gttgactgg agtccggag cacccaacg 
      gtcattgcg accattcga ggtccagat atactaata 
      tgggatctt caaaggaag atgcatcag tcgcagtct 
      atcagcaag acgcctcat aatattttt aacaccgtc 
      tgcaggttt acgcccaat ggtcgtagt ttaatggtc 
      ggcgatatg aaggggaat gtgcatgtg tactgtctg 
      gaggatatg cccataccg ccgttcttc cccgagaat 
      cttatctcg caaagtttg aaaaaactc ttggttacg 
      agaccggat ttgatgaag aagttgcgc aagttgggg 
      tcgttgaag ttcgacgat aggcagttt tcgaaatat 
      tttaattaa
    mRNA sequence
    • >TC034331 RA
      ttttttggc taaaaatgc tgcgaaaaa tcccaaaac 
      atgtgacta ttgtgttaa acgagacgg aaacccttt 
      tcttgcttg aacttccaa gttctgtgg tcccgcggg 
      acactgacg agggcgaag ccgccgaga tggacaacc 
      gcctttacg aatacttaa caaagggta aagggcgta 
      atcgcaaag ttgcaacaa ctggagtac aaacgaggc 
      ctgttttaa tgaaaacgc gaaacacgg aacccgtcc 
      agtacaagt tcagggaca actcaacgt ttgcttcaa 
      actgggaga tgtatgata cctacaatg ccagttttg 
      ttgaaagtg ttgagttgg aggaaacaa acgaccatg 
      agttaattg cggaagtgt ctttggatg aactaagga 
      agagtcgag agaccaaag agttggaga agttgtcag 
      tgaataaaa actacttcg acgctgttc tagtaattg 
      aacgccttt tggccaaca attgttaca acaacgaac 
      agaagaagt tcaaagggt tggtggacg aggccgagg 
      aaatccgct acgattaca agctggact tgctgtgga 
      ctttcgcca acgaagaca cgaaaaata ggtgcgtta 
      ccgggatct cgttcaatt ctttcaccg aagatatgg 
      tagctgtgg gctatggca agtactatt ttattgacc 
      ggagaaaga ccggggttg tgatggtgt ggaacataa 
      aaaatccca aacagcccg agcgcgagt actacttca 
      gacacccgg tcacgtccc ttgcgtttt caacaaaag 
      acccgaact atctggctg ttgggttct acaatgggg 
      agctcctag ttatagatg tcagcaaaa gggactttg 
      agatggttg gggaggact ctagcactt cgtttaaac 
      cggcctgga acatggtat ggctggagc aaaaccaga 
      aggaatttc tcctggttt gtttcggcg acggtcggg 
      tttgcaagt acttggtga cccaagcga agctcgagt 
      gtgtacaaa ttatgagga cggataaaa ttgaaggga 
      agctgaaag ggttaccag ccttgcggt tgtttgaag 
      gcaatcagg tttcgtcgt ctaggtatg ccggcgctc 
      tttgcttaa gtgtgcaca agccggacc ctgccttct 
      accttgtgg gtacagagg aaggtgtca tacacaagt 
      gtgccgcaa gttactgta cccaccatg tggacatgt 
      tcaaagcgc atgaaggcc ccattcaca ctattaagt 
      tttcaccat ttgttgaaa aagtgtttg cgacctgtg 
      gtgacgact ttcagaagt gtttgtgga tcgagggga 
      ttagtgagc ccttgattg tctcgcggt tttgcatgg 
      agccggtgc ttgatgtcg actggagtc cgtcacact 
      cgactatca ttgcaagtc ttcgcggca tggacatct 
      tcctgtggg acttgcaaa ggaagatct acttgccac 
      agtcccaaa ctaagtcac ccacgaatt caagaaaca 
      cggtttgta agtttagtg cttgtggca agtgtttag 
      tggtcgggg acattgacg gtaatgtcc acacgtttg 
      ctgtgagcg ggctaccaa ttcctgcct tctttcaac 
      aaaacctat ttttccagt cgattaaaa acatcctgc 
      aaaccaata cggatctaa tcggcaaac tcgaaaatt 
      ggcaaattg acaatccct gtcaaaatc tgatggctt 
      cttcgcccc gaaccggcc gttcggtca ccaaatcca 
      gcgtttcgt acgaccctg gctaccaaa gcagcctcc 
      gcaaactgc gcggcaaag accgggaat ctctccgcc 
      ccaaaacaa tcctcaccg cccccaaat accaagtca 
      tccccgtga ttttcgatg gcgtggacg tcaccccca 
      tatccctca acccctacg tcttcgagg gcgggcgcg 
      agcgccaga tcagcatca tggacaacc tgaccaaag 
      acagcttca tgctgcacc accactcgc tcagccgag 
      tcgaatccc aaaagttcc aaaccaaca tcctgctcc 
      agaagcagg aaggcgaga ctgcgacct tcacgcacc 
      ggagcatgg taacttcgg cctacttcg gcgacgaaa 
      tcctcctcc cggatgtga gtttgagga gcaaatcgg 
      agttttcgc agcaggaga ttgtgtacg atgaggagt 
      cggtggaag ggaagcctg aactgccga tcccggagc 
      atgttagtc tggtcctgg tggagaccg agaccatgt 
      tcctgctgg atttgccga gttcgaccg tttcgaaga 
      caagtgaag gggccaacg atgttgagg aggacaaca 
      agttgtacg agtttctga ccaagggga aggggcgca 
      atcggaagg tgatagcag tggggatac aaactgtgc 
      cgattttga agaaagcaa gggacacgg agacgaaga 
      agatgaggt ttaggaaca attccacgt acgtttccg 
      cctgggaga tgtacgata cgttccaag agttggagg 
      aggaagccc atgaggagt tcgaagata agttgagtg 
      atgaagaaa tggggactt tggttgagg aagagcaca 
      ggttgaacg acgctgcgt cccttgaga gttaccgga 
      tgtccgccg aagacaaac agttgaaca aaattgtga 
      ggagtaaag ctttccatg atgccctta ttgtcattg 
      aacgcatcc ttgggaata actgttaca acgcgcagc 
      aaaagcgct acaaaggcc tttcggagc cggacgtct 
      tccgcgaaa acatcgaat acaagtaca ggttggaac 
      ttctatgga cttacgcga atgatgaaa ccgactcca 
      gatgtgtca ccggagtgg ccttcaatt ccttcaacg 
      aagacttag ttgcagtgg cgtacggca agtacttct 
      acactgacc ggagcgcaa ccggaatgg tgatgatct 
      ggaacatca aaaatccgc aacagccgg aacgccagt 
      acgactttg aaacggccg taacatctc ttgcgtttt 
      ctaaagtga acccgaatt tgctcgctg tggggttct 
      acaacggcg atttgaaaa ttctggacg ttagtaagc 
      gggaaattt cggcgattc gggaagacg atacgtttt 
      tcgaacccg tctggagca tcctttggt tccagcaag 
      gcggggatg aggacctta tggttagtt tcggggacg 
      gccgtgtgt gtcgctacc aaataacga cagctcggg 
      atttgaaac ggacgcaga ttatgataa ctgccgcgg 
      ccgaaggga agttgaaag ggatcaagt atttgaaaa 
      aatgtgaag ggaagaata tctccattt cgggatatg 
      accaagcca ggtgtttgg cgatgcacc cgtgtgacc 
      ccttcactt acttcgtcg ggaccagtg aaggggtgg 
      ttcatagat gctccacta actactaca acgagcaca 
      tggacctgt tcaaggccc atgatggtg caatccaca 
      ccatacgtt tctcccctt ttgccaaca agttgtact 
      acacttgtg gtgacgatt ttagggcgc gtctttggg 
      tagacggta tttcggaat gtttggtaa cttcggaac 
      cgtccatgt taccagtgc tggacgttg actggagtc 
      cggagcacc caacggtca ttgcgacca ttcgaggtc 
      cagatatac taatatggg atcttcaaa ggaagatgc 
      atcagtcgc agtctatca gcaagacgc ctcataata 
      tttttaaca ccgtctgca ggtttacgc ccaatggtc 
      gtagtttaa tggtcggcg atatgaagg ggaatgtgc 
      atgtgtact gtctggagg atatgccca taccgccgt 
      tcttccccg agaatctta tctcgcaaa gtttgaaaa 
      aactcttgg ttacgagac cggatttga tgaagaagt 
      tgcgcaagt tggggtcgt tgaagttcg acgataggc 
      agttttcga aatatttta attaattca taagattaa 
      ttaattgtg agaaaatat atcttactt aaaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY