| General information | |||
| TC012422 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 7099949..7114671 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012422 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05239 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
- >TC012422 RA
MDPYEQQLL TVFDSYDHE HLGSLDRDG LTQLCQTLQ LEEHSSDLI QCLLTDKHP RATFAEFKD ALLTLLDTM QHRKNTDDS KKTESPDRE VSPKFIYGS KKYGRRSRP RSDEITNML DEQNDLNYL NKNSNAVQR SNSQSEVSN SKKRKTNYK LKRCTSLPA TQRNFVANN MNNETEFVY TEEMLREAW KKLEVGKDG YLNQNELVL VCDAIGLHN LAKGVMRQL SDRLSVDYD KISFQELLE ALQMDETWT EVLNSPVND VTLTVTNEI FPDSRTFQY VTLGPDGNG VINAETLIE MWELVGISS PKELIHELG FNDREINII ELATVLEKE IKGIHDSSR LEMQNPHIA LLHANLTLY QSEIKCLKH VLEQMHAER EKLKCDVFE ANNRAKLLA QEVDDNHVK MERNTQNQV RLIEQRHSD ILKEITQQY AGDKEHLSA LNQTLEAKI CSLEQEVNK LKNDLVVAQ KYSSTLEKE NQNLSNQIS ELQQVKSLL SDQINSLEC EKQKYVEME QEQIEPLLT KLSTLQLEN AQLRDRNDE MVAEIESLT CQVSSMRAK VASTPTYNT LDQSMEENI SVVCEGVGL GAKRRSDYS PTKDGVLFG IADGSPRLG KVRKFHKER PDHLDVVPF TSNESGFEA EIDAFDSSF SGTDEEITR LQAKVAFLE HTLAQHSIP IPGDYAVEN ATSDTINSL RARVQELEK LFGDLSDVS KMINKNGLS CDNLKSVGE KLEMILSQR NEKVFSDAN VGTDTDLVS FEHRVKQYE EENEMLKTK CSELENCVE LLKNEYEKC EDYWQSKLE EERQIFEQE QSQSSEKLT DLINKMAEY EEQFANQDT LDSRLPPIE ETYNLEKQF TDLEQEFED YKLQSENEL FKRDEEISI LKQKLTDLA LSQKDSAEM GTQVNSLDE NSRISTKMN SLSLCMVES TNLFSSDAM PVPWSPEKK PEMVDWGKE SRSDTNSTQ TSLSWQNGS GQPSTSNSS TLEKNSTPC RPKRTRKHD RNNCLYKKN SQEMKKSDD FPSRREEQV VTLPVSTIH GLNGRLHHL EQRCRQLQM VLKQQHFYT EQALQQCRQ HHKSEKTEL QCILRSHQE KLDQQVRIC NDQLERLAR SDMLVKELY VENSYLIAK VQRLEQQCH ILAQCNSNS SSV
- >TC012422 RA
atggatccc tacgagcag caactccta accgtcttc gacagctac gaccacgag cacctgggc agcttagac cgcgacggt ctcacccag ctctgccaa accctccaa ctcgaagag cactccagc gacctgatc caatgcctc ctcaccgac aagcacccg cgcgccacc ttcgccgaa ttcaaggac gccctcctc accctcctc gacaccatg cagcaccgg aaaaacacc gacgattcg aaaaaaacc gaatctccg gaccgcgaa gtctccccg aaattcatc tacggctcg aagaaatac ggccgccgg tcgcgccct cgcagcgac gaaatcaca aacatgctc gacgaacag aacgaccta aattacctc aacaaaaac tcaaacgcc gtccagcgg tccaattcc cagagcgaa gtctccaac tccaaaaaa cgcaaaacc aactacaaa ctcaaacgc tgcacctct ctgccggca acgcagcga aacttcgtc gctaataat atgaacaac gagactgag tttgtctac acggaggag atgttgagg gaagcgtgg aagaaactc gaggttggg aaagacggt tacttgaac cagaacgag ttggttcta gtgtgcgat gccatcggg ttgcataac ctggccaag ggggtgatg aggcaactg tctgatagg ctgagtgtt gactatgac aagatcagt tttcaagag ttgctagag gccttgcaa atggacgag acctggacc gaagtcctg aactcgccg gttaatgac gtcacttta accgtaacc aatgagatt ttcccggat tcgcgcaca ttccagtac gttacgttg ggtcctgac ggcaacggg gtcataaat gccgaaacg ttgatcgag atgtgggaa ctagtgggg atcagttcg cccaaagaa ctgatccac gagctgggt ttcaacgac cgtgagata aacatcatc gagttggcg acggtgcta gagaaggaa attaaaggc atccatgac tcctcaagg ctggaaatg caaaacccc cacattgcc cttttgcac gccaacttg acgctgtac cagtccgag atcaagtgc ttgaagcac gtcctggag cagatgcac gccgagcgg gagaaactg aaatgcgac gtgtttgag gcaaacaac cgggccaag ttactggcc caggaagtg gacgataac cacgtgaaa atggagcgt aacacccag aaccaagtc aggctgatc gagcagaga cactccgac atactcaag gagataacg cagcaatat gccggggat aaggagcac ctttccgcc ctcaaccag accctcgag gcgaaaatt tgctccctg gagcaagaa gtcaataaa ttgaagaac gacctagtc gtggcccaa aaatactcc tcgactttg gagaaggaa aaccagaac ttgtccaac caaatttcc gagttgcag caagtcaag agcttgctc tcggaccag atcaactcc ctcgagtgc gagaagcag aagtacgtg gagatggag caggaacag atcgagccg ttgttgacg aagttgtcc actttgcag ctggagaac gcgcagttg agggaccgg aacgacgaa atggtggcg gagattgag agcttgacg tgccaggtg tcgagtatg agggccaaa gtggccagc acgcccacg tacaacacc ctggaccag agcatggag gagaatatt tctgttgtt tgcgaagga gtcggtttg ggtgcgaag cgtaggagc gattattcg ccgaccaag gacggcgtt ctcttcggt atagccgac ggcagtccc cggctcggc aaggtgcga aaattccac aaggagcgg cccgaccac ttggacgtc gtgcccttc acgtcgaac gagagcggg ttcgaggcc gaaatcgat gccttcgac agctcgttt tcgggaacc gacgaagag atcaccagg ttgcaggcc aaggtcgcg ttcctcgaa cacacgctc gcccagcat tcgattccg atacccggt gattacgcg gtggaaaat gccacctct gacacaata aatagtctt agagcgaga gtgcaagaa ttagagaaa ctttttggt gacttgagc gatgttagt aaaatgata aacaagaac ggcttaagt tgtgataat ttgaaaagt gttggtgaa aaactcgag atgattctg agccaacga aacgaaaaa gttttcagc gacgctaat gtcgggact gacacagac ttggtttca ttcgaacac cgggtaaaa caatacgag gaagagaac gaaatgttg aaaaccaaa tgttccgaa ctggaaaac tgtgtggaa cttctcaag aacgaatac gagaagtgc gaagactac tggcagtcc aaactggag gaagagcgc caaatcttc gaacaggaa cagagccaa agtagcgaa aaactcacc gacttgatc aacaaaatg gccgagtac gaggaacag ttcgccaat caggacacc ctcgacagc agattgccc ccaatcgaa gaaacctac aatttggag aaacaattc accgacctg gagcaagaa ttcgaagat tacaagctc caaagcgag aacgaactg ttcaaacgc gacgaagaa atctctatt ttgaagcaa aaactgacc gatctggcc ctgagtcag aaagacagt gccgaaatg gggacccag gtcaacagt ctggacgaa aactcccgc ataagcaca aaaatgaac agtctgtcc ctgtgcatg gtcgagtcc acgaactta ttttcctcc gatgcgatg ccagttccg tggtcccct gagaaaaag ccagaaatg gtggactgg ggtaaggag tcgagaagt gacacaaac tcgactcag acctcgctg tcgtggcag aacggtagt gggcagccg tcaacgtcg aactcgtcc actttggag aaaaactcc acgccttgt aggcccaag cggacgcgg aagcacgac cggaataat tgtctctac aagaaaaac agtcaggag atgaagaag agtgacgat tttccctcc agacgggag gagcaagtt gttacgtta cctgtgagt acaattcat ggcttgaac ggccggttg caccacctg gagcagagg tgcaggcag ctgcagatg gtgctgaag cagcagcat ttttacacg gaacaggcc ttgcaacag tgtcggcag catcacaag agtgagaag accgaattg cagtgcatt ttgcgcagt catcaagag aagttggac caacaagtg aggatctgc aacgatcag ttggagaga ctcgccagg tcagatatg ttagtcaag gagttgtat gtggagaat tcgtatctg attgcaaaa gtacaaaga cttgagcaa cagtgccat atcttggca cagtgtaac tcgaatagt agttcggtg tag
- >TC012422 RA
ccaaacata caattcgag agtgtaact gtatttttt cacttttaa cgatgtaat attaataag gggattacc ataacttgg tctagttcc agcaaatta tatcaccag tgacattga atttcgtgt gtccagcct gttttcccc aaacccgac ccacttttt ttgttgtga ttgtgttat tgtgacatt tgtgacgtt tcactagaa attgcagta aaaataccg ttatgttta ctaaatttc gttaaattt tagcaaacc caataaatt ctcttcgtt tacgatgcc ataaatcgt gtccaagtc agggtacgc aacaccatg gatccctac gagcagcaa ctcctaacc gtcttcgac agctacgac cacgagcac ctgggcagc ttagaccgc gacggtctc acccagctc tgccaaacc ctccaactc gaagagcac tccagcgac ctgatccaa tgcctcctc accgacaag cacccgcgc gccaccttc gccgaattc aaggacgcc ctcctcacc ctcctcgac accatgcag caccggaaa aacaccgac gattcgaaa aaaaccgaa tctccggac cgcgaagtc tccccgaaa ttcatctac ggctcgaag aaatacggc cgccggtcg cgccctcgc agcgacgaa atcacaaac atgctcgac gaacagaac gacctaaat tacctcaac aaaaactca aacgccgtc cagcggtcc aattcccag agcgaagtc tccaactcc aaaaaacgc aaaaccaac tacaaactc aaacgctgc acctctctg ccggcaacg cagcgaaac ttcgtcgct aataatatg aacaacgag actgagttt gtctacacg gaggagatg ttgagggaa gcgtggaag aaactcgag gttgggaaa gacggttac ttgaaccag aacgagttg gttctagtg tgcgatgcc atcgggttg cataacctg gccaagggg gtgatgagg caactgtct gataggctg agtgttgac tatgacaag atcagtttt caagagttg ctagaggcc ttgcaaatg gacgagacc tggaccgaa gtcctgaac tcgccggtt aatgacgtc actttaacc gtaaccaat gagattttc ccggattcg cgcacattc cagtacgtt acgttgggt cctgacggc aacggggtc ataaatgcc gaaacgttg atcgagatg tgggaacta gtggggatc agttcgccc aaagaactg atccacgag ctgggtttc aacgaccgt gagataaac atcatcgag ttggcgacg gtgctagag aaggaaatt aaaggcatc catgactcc tcaaggctg gaaatgcaa aacccccac attgccctt ttgcacgcc aacttgacg ctgtaccag tccgagatc aagtgcttg aagcacgtc ctggagcag atgcacgcc gagcgggag aaactgaaa tgcgacgtg tttgaggca aacaaccgg gccaagtta ctggcccag gaagtggac gataaccac gtgaaaatg gagcgtaac acccagaac caagtcagg ctgatcgag cagagacac tccgacata ctcaaggag ataacgcag caatatgcc ggggataag gagcacctt tccgccctc aaccagacc ctcgaggcg aaaatttgc tccctggag caagaagtc aataaattg aagaacgac ctagtcgtg gcccaaaaa tactcctcg actttggag aaggaaaac cagaacttg tccaaccaa atttccgag ttgcagcaa gtcaagagc ttgctctcg gaccagatc aactccctc gagtgcgag aagcagaag tacgtggag atggagcag gaacagatc gagccgttg ttgacgaag ttgtccact ttgcagctg gagaacgcg cagttgagg gaccggaac gacgaaatg gtggcggag attgagagc ttgacgtgc caggtgtcg agtatgagg gccaaagtg gccagcacg cccacgtac aacaccctg gaccagagc atggaggag aatatttct gttgtttgc gaaggagtc ggtttgggt gcgaagcgt aggagcgat tattcgccg accaaggac ggcgttctc ttcggtata gccgacggc agtccccgg ctcggcaag gtgcgaaaa ttccacaag gagcggccc gaccacttg gacgtcgtg cccttcacg tcgaacgag agcgggttc gaggccgaa atcgatgcc ttcgacagc tcgttttcg ggaaccgac gaagagatc accaggttg caggccaag gtcgcgttc ctcgaacac acgctcgcc cagcattcg attccgata cccggtgat tacgcggtg gaaaatgcc acctctgac acaataaat agtcttaga gcgagagtg caagaatta gagaaactt tttggtgac ttgagcgat gttagtaaa atgataaac aagaacggc ttaagttgt gataatttg aaaagtgtt ggtgaaaaa ctcgagatg attctgagc caacgaaac gaaaaagtt ttcagcgac gctaatgtc gggactgac acagacttg gtttcattc gaacaccgg gtaaaacaa tacgaggaa gagaacgaa atgttgaaa accaaatgt tccgaactg gaaaactgt gtggaactt ctcaagaac gaatacgag aagtgcgaa gactactgg cagtccaaa ctggaggaa gagcgccaa atcttcgaa caggaacag agccaaagt agcgaaaaa ctcaccgac ttgatcaac aaaatggcc gagtacgag gaacagttc gccaatcag gacaccctc gacagcaga ttgccccca atcgaagaa acctacaat ttggagaaa caattcacc gacctggag caagaattc gaagattac aagctccaa agcgagaac gaactgttc aaacgcgac gaagaaatc tctattttg aagcaaaaa ctgaccgat ctggccctg agtcagaaa gacagtgcc gaaatgggg acccaggtc aacagtctg gacgaaaac tcccgcata agcacaaaa atgaacagt ctgtccctg tgcatggtc gagtccacg aacttattt tcctccgat gcgatgcca gttccgtgg tcccctgag aaaaagcca gaaatggtg gactggggt aaggagtcg agaagtgac acaaactcg actcagacc tcgctgtcg tggcagaac ggtagtggg cagccgtca acgtcgaac tcgtccact ttggagaaa aactccacg ccttgtagg cccaagcgg acgcggaag cacgaccgg aataattgt ctctacaag aaaaacagt caggagatg aagaagagt gacgatttt ccctccaga cgggaggag caagttgtt acgttacct gtgagtaca attcatggc ttgaacggc cggttgcac cacctggag cagaggtgc aggcagctg cagatggtg ctgaagcag cagcatttt tacacggaa caggccttg caacagtgt cggcagcat cacaagagt gagaagacc gaattgcag tgcattttg cgcagtcat caagagaag ttggaccaa caagtgagg atctgcaac gatcagttg gagagactc gccaggtca gatatgtta gtcaaggag ttgtatgtg gagaattcg tatctgatt gcaaaagta caaagactt gagcaacag tgccatatc ttggcacag tgtaactcg aatagtagt tcggtgtag ttggagcct atttttgcc taattgtag cgatagttg actattttt aaagactaa atatttaat tttgtgtga gtttttttt tgtatattc tgattgatt gttaaatta cctgactag agaattgta agagtaata atgttatgg gttgtttat tcttttata tgatgtact tttgttaca tatcattta tttttactt atatccaaa gaatttaac acgataagt atctagtta ctgttacta ttaactact aatttttaa agtgtcata ttattgata aataaagta acgtcaaac at
