Status
Please wait ...
Gene information
General information
TC012422 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7099949..7114671
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012422
    iBeetle screen iB_05239

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012422 RA
      MDPYEQQLL TVFDSYDHE HLGSLDRDG LTQLCQTLQ 
      LEEHSSDLI QCLLTDKHP RATFAEFKD ALLTLLDTM 
      QHRKNTDDS KKTESPDRE VSPKFIYGS KKYGRRSRP 
      RSDEITNML DEQNDLNYL NKNSNAVQR SNSQSEVSN 
      SKKRKTNYK LKRCTSLPA TQRNFVANN MNNETEFVY 
      TEEMLREAW KKLEVGKDG YLNQNELVL VCDAIGLHN 
      LAKGVMRQL SDRLSVDYD KISFQELLE ALQMDETWT 
      EVLNSPVND VTLTVTNEI FPDSRTFQY VTLGPDGNG 
      VINAETLIE MWELVGISS PKELIHELG FNDREINII 
      ELATVLEKE IKGIHDSSR LEMQNPHIA LLHANLTLY 
      QSEIKCLKH VLEQMHAER EKLKCDVFE ANNRAKLLA 
      QEVDDNHVK MERNTQNQV RLIEQRHSD ILKEITQQY 
      AGDKEHLSA LNQTLEAKI CSLEQEVNK LKNDLVVAQ 
      KYSSTLEKE NQNLSNQIS ELQQVKSLL SDQINSLEC 
      EKQKYVEME QEQIEPLLT KLSTLQLEN AQLRDRNDE 
      MVAEIESLT CQVSSMRAK VASTPTYNT LDQSMEENI 
      SVVCEGVGL GAKRRSDYS PTKDGVLFG IADGSPRLG 
      KVRKFHKER PDHLDVVPF TSNESGFEA EIDAFDSSF 
      SGTDEEITR LQAKVAFLE HTLAQHSIP IPGDYAVEN 
      ATSDTINSL RARVQELEK LFGDLSDVS KMINKNGLS 
      CDNLKSVGE KLEMILSQR NEKVFSDAN VGTDTDLVS 
      FEHRVKQYE EENEMLKTK CSELENCVE LLKNEYEKC 
      EDYWQSKLE EERQIFEQE QSQSSEKLT DLINKMAEY 
      EEQFANQDT LDSRLPPIE ETYNLEKQF TDLEQEFED 
      YKLQSENEL FKRDEEISI LKQKLTDLA LSQKDSAEM 
      GTQVNSLDE NSRISTKMN SLSLCMVES TNLFSSDAM 
      PVPWSPEKK PEMVDWGKE SRSDTNSTQ TSLSWQNGS 
      GQPSTSNSS TLEKNSTPC RPKRTRKHD RNNCLYKKN 
      SQEMKKSDD FPSRREEQV VTLPVSTIH GLNGRLHHL 
      EQRCRQLQM VLKQQHFYT EQALQQCRQ HHKSEKTEL 
      QCILRSHQE KLDQQVRIC NDQLERLAR SDMLVKELY 
      VENSYLIAK VQRLEQQCH ILAQCNSNS SSV
    CDS sequence
    • >TC012422 RA
      atggatccc tacgagcag caactccta accgtcttc 
      gacagctac gaccacgag cacctgggc agcttagac 
      cgcgacggt ctcacccag ctctgccaa accctccaa 
      ctcgaagag cactccagc gacctgatc caatgcctc 
      ctcaccgac aagcacccg cgcgccacc ttcgccgaa 
      ttcaaggac gccctcctc accctcctc gacaccatg 
      cagcaccgg aaaaacacc gacgattcg aaaaaaacc 
      gaatctccg gaccgcgaa gtctccccg aaattcatc 
      tacggctcg aagaaatac ggccgccgg tcgcgccct 
      cgcagcgac gaaatcaca aacatgctc gacgaacag 
      aacgaccta aattacctc aacaaaaac tcaaacgcc 
      gtccagcgg tccaattcc cagagcgaa gtctccaac 
      tccaaaaaa cgcaaaacc aactacaaa ctcaaacgc 
      tgcacctct ctgccggca acgcagcga aacttcgtc 
      gctaataat atgaacaac gagactgag tttgtctac 
      acggaggag atgttgagg gaagcgtgg aagaaactc 
      gaggttggg aaagacggt tacttgaac cagaacgag 
      ttggttcta gtgtgcgat gccatcggg ttgcataac 
      ctggccaag ggggtgatg aggcaactg tctgatagg 
      ctgagtgtt gactatgac aagatcagt tttcaagag 
      ttgctagag gccttgcaa atggacgag acctggacc 
      gaagtcctg aactcgccg gttaatgac gtcacttta 
      accgtaacc aatgagatt ttcccggat tcgcgcaca 
      ttccagtac gttacgttg ggtcctgac ggcaacggg 
      gtcataaat gccgaaacg ttgatcgag atgtgggaa 
      ctagtgggg atcagttcg cccaaagaa ctgatccac 
      gagctgggt ttcaacgac cgtgagata aacatcatc 
      gagttggcg acggtgcta gagaaggaa attaaaggc 
      atccatgac tcctcaagg ctggaaatg caaaacccc 
      cacattgcc cttttgcac gccaacttg acgctgtac 
      cagtccgag atcaagtgc ttgaagcac gtcctggag 
      cagatgcac gccgagcgg gagaaactg aaatgcgac 
      gtgtttgag gcaaacaac cgggccaag ttactggcc 
      caggaagtg gacgataac cacgtgaaa atggagcgt 
      aacacccag aaccaagtc aggctgatc gagcagaga 
      cactccgac atactcaag gagataacg cagcaatat 
      gccggggat aaggagcac ctttccgcc ctcaaccag 
      accctcgag gcgaaaatt tgctccctg gagcaagaa 
      gtcaataaa ttgaagaac gacctagtc gtggcccaa 
      aaatactcc tcgactttg gagaaggaa aaccagaac 
      ttgtccaac caaatttcc gagttgcag caagtcaag 
      agcttgctc tcggaccag atcaactcc ctcgagtgc 
      gagaagcag aagtacgtg gagatggag caggaacag 
      atcgagccg ttgttgacg aagttgtcc actttgcag 
      ctggagaac gcgcagttg agggaccgg aacgacgaa 
      atggtggcg gagattgag agcttgacg tgccaggtg 
      tcgagtatg agggccaaa gtggccagc acgcccacg 
      tacaacacc ctggaccag agcatggag gagaatatt 
      tctgttgtt tgcgaagga gtcggtttg ggtgcgaag 
      cgtaggagc gattattcg ccgaccaag gacggcgtt 
      ctcttcggt atagccgac ggcagtccc cggctcggc 
      aaggtgcga aaattccac aaggagcgg cccgaccac 
      ttggacgtc gtgcccttc acgtcgaac gagagcggg 
      ttcgaggcc gaaatcgat gccttcgac agctcgttt 
      tcgggaacc gacgaagag atcaccagg ttgcaggcc 
      aaggtcgcg ttcctcgaa cacacgctc gcccagcat 
      tcgattccg atacccggt gattacgcg gtggaaaat 
      gccacctct gacacaata aatagtctt agagcgaga 
      gtgcaagaa ttagagaaa ctttttggt gacttgagc 
      gatgttagt aaaatgata aacaagaac ggcttaagt 
      tgtgataat ttgaaaagt gttggtgaa aaactcgag 
      atgattctg agccaacga aacgaaaaa gttttcagc 
      gacgctaat gtcgggact gacacagac ttggtttca 
      ttcgaacac cgggtaaaa caatacgag gaagagaac 
      gaaatgttg aaaaccaaa tgttccgaa ctggaaaac 
      tgtgtggaa cttctcaag aacgaatac gagaagtgc 
      gaagactac tggcagtcc aaactggag gaagagcgc 
      caaatcttc gaacaggaa cagagccaa agtagcgaa 
      aaactcacc gacttgatc aacaaaatg gccgagtac 
      gaggaacag ttcgccaat caggacacc ctcgacagc 
      agattgccc ccaatcgaa gaaacctac aatttggag 
      aaacaattc accgacctg gagcaagaa ttcgaagat 
      tacaagctc caaagcgag aacgaactg ttcaaacgc 
      gacgaagaa atctctatt ttgaagcaa aaactgacc 
      gatctggcc ctgagtcag aaagacagt gccgaaatg 
      gggacccag gtcaacagt ctggacgaa aactcccgc 
      ataagcaca aaaatgaac agtctgtcc ctgtgcatg 
      gtcgagtcc acgaactta ttttcctcc gatgcgatg 
      ccagttccg tggtcccct gagaaaaag ccagaaatg 
      gtggactgg ggtaaggag tcgagaagt gacacaaac 
      tcgactcag acctcgctg tcgtggcag aacggtagt 
      gggcagccg tcaacgtcg aactcgtcc actttggag 
      aaaaactcc acgccttgt aggcccaag cggacgcgg 
      aagcacgac cggaataat tgtctctac aagaaaaac 
      agtcaggag atgaagaag agtgacgat tttccctcc 
      agacgggag gagcaagtt gttacgtta cctgtgagt 
      acaattcat ggcttgaac ggccggttg caccacctg 
      gagcagagg tgcaggcag ctgcagatg gtgctgaag 
      cagcagcat ttttacacg gaacaggcc ttgcaacag 
      tgtcggcag catcacaag agtgagaag accgaattg 
      cagtgcatt ttgcgcagt catcaagag aagttggac 
      caacaagtg aggatctgc aacgatcag ttggagaga 
      ctcgccagg tcagatatg ttagtcaag gagttgtat 
      gtggagaat tcgtatctg attgcaaaa gtacaaaga 
      cttgagcaa cagtgccat atcttggca cagtgtaac 
      tcgaatagt agttcggtg tag
    mRNA sequence
    • >TC012422 RA
      ccaaacata caattcgag agtgtaact gtatttttt 
      cacttttaa cgatgtaat attaataag gggattacc 
      ataacttgg tctagttcc agcaaatta tatcaccag 
      tgacattga atttcgtgt gtccagcct gttttcccc 
      aaacccgac ccacttttt ttgttgtga ttgtgttat 
      tgtgacatt tgtgacgtt tcactagaa attgcagta 
      aaaataccg ttatgttta ctaaatttc gttaaattt 
      tagcaaacc caataaatt ctcttcgtt tacgatgcc 
      ataaatcgt gtccaagtc agggtacgc aacaccatg 
      gatccctac gagcagcaa ctcctaacc gtcttcgac 
      agctacgac cacgagcac ctgggcagc ttagaccgc 
      gacggtctc acccagctc tgccaaacc ctccaactc 
      gaagagcac tccagcgac ctgatccaa tgcctcctc 
      accgacaag cacccgcgc gccaccttc gccgaattc 
      aaggacgcc ctcctcacc ctcctcgac accatgcag 
      caccggaaa aacaccgac gattcgaaa aaaaccgaa 
      tctccggac cgcgaagtc tccccgaaa ttcatctac 
      ggctcgaag aaatacggc cgccggtcg cgccctcgc 
      agcgacgaa atcacaaac atgctcgac gaacagaac 
      gacctaaat tacctcaac aaaaactca aacgccgtc 
      cagcggtcc aattcccag agcgaagtc tccaactcc 
      aaaaaacgc aaaaccaac tacaaactc aaacgctgc 
      acctctctg ccggcaacg cagcgaaac ttcgtcgct 
      aataatatg aacaacgag actgagttt gtctacacg 
      gaggagatg ttgagggaa gcgtggaag aaactcgag 
      gttgggaaa gacggttac ttgaaccag aacgagttg 
      gttctagtg tgcgatgcc atcgggttg cataacctg 
      gccaagggg gtgatgagg caactgtct gataggctg 
      agtgttgac tatgacaag atcagtttt caagagttg 
      ctagaggcc ttgcaaatg gacgagacc tggaccgaa 
      gtcctgaac tcgccggtt aatgacgtc actttaacc 
      gtaaccaat gagattttc ccggattcg cgcacattc 
      cagtacgtt acgttgggt cctgacggc aacggggtc 
      ataaatgcc gaaacgttg atcgagatg tgggaacta 
      gtggggatc agttcgccc aaagaactg atccacgag 
      ctgggtttc aacgaccgt gagataaac atcatcgag 
      ttggcgacg gtgctagag aaggaaatt aaaggcatc 
      catgactcc tcaaggctg gaaatgcaa aacccccac 
      attgccctt ttgcacgcc aacttgacg ctgtaccag 
      tccgagatc aagtgcttg aagcacgtc ctggagcag 
      atgcacgcc gagcgggag aaactgaaa tgcgacgtg 
      tttgaggca aacaaccgg gccaagtta ctggcccag 
      gaagtggac gataaccac gtgaaaatg gagcgtaac 
      acccagaac caagtcagg ctgatcgag cagagacac 
      tccgacata ctcaaggag ataacgcag caatatgcc 
      ggggataag gagcacctt tccgccctc aaccagacc 
      ctcgaggcg aaaatttgc tccctggag caagaagtc 
      aataaattg aagaacgac ctagtcgtg gcccaaaaa 
      tactcctcg actttggag aaggaaaac cagaacttg 
      tccaaccaa atttccgag ttgcagcaa gtcaagagc 
      ttgctctcg gaccagatc aactccctc gagtgcgag 
      aagcagaag tacgtggag atggagcag gaacagatc 
      gagccgttg ttgacgaag ttgtccact ttgcagctg 
      gagaacgcg cagttgagg gaccggaac gacgaaatg 
      gtggcggag attgagagc ttgacgtgc caggtgtcg 
      agtatgagg gccaaagtg gccagcacg cccacgtac 
      aacaccctg gaccagagc atggaggag aatatttct 
      gttgtttgc gaaggagtc ggtttgggt gcgaagcgt 
      aggagcgat tattcgccg accaaggac ggcgttctc 
      ttcggtata gccgacggc agtccccgg ctcggcaag 
      gtgcgaaaa ttccacaag gagcggccc gaccacttg 
      gacgtcgtg cccttcacg tcgaacgag agcgggttc 
      gaggccgaa atcgatgcc ttcgacagc tcgttttcg 
      ggaaccgac gaagagatc accaggttg caggccaag 
      gtcgcgttc ctcgaacac acgctcgcc cagcattcg 
      attccgata cccggtgat tacgcggtg gaaaatgcc 
      acctctgac acaataaat agtcttaga gcgagagtg 
      caagaatta gagaaactt tttggtgac ttgagcgat 
      gttagtaaa atgataaac aagaacggc ttaagttgt 
      gataatttg aaaagtgtt ggtgaaaaa ctcgagatg 
      attctgagc caacgaaac gaaaaagtt ttcagcgac 
      gctaatgtc gggactgac acagacttg gtttcattc 
      gaacaccgg gtaaaacaa tacgaggaa gagaacgaa 
      atgttgaaa accaaatgt tccgaactg gaaaactgt 
      gtggaactt ctcaagaac gaatacgag aagtgcgaa 
      gactactgg cagtccaaa ctggaggaa gagcgccaa 
      atcttcgaa caggaacag agccaaagt agcgaaaaa 
      ctcaccgac ttgatcaac aaaatggcc gagtacgag 
      gaacagttc gccaatcag gacaccctc gacagcaga 
      ttgccccca atcgaagaa acctacaat ttggagaaa 
      caattcacc gacctggag caagaattc gaagattac 
      aagctccaa agcgagaac gaactgttc aaacgcgac 
      gaagaaatc tctattttg aagcaaaaa ctgaccgat 
      ctggccctg agtcagaaa gacagtgcc gaaatgggg 
      acccaggtc aacagtctg gacgaaaac tcccgcata 
      agcacaaaa atgaacagt ctgtccctg tgcatggtc 
      gagtccacg aacttattt tcctccgat gcgatgcca 
      gttccgtgg tcccctgag aaaaagcca gaaatggtg 
      gactggggt aaggagtcg agaagtgac acaaactcg 
      actcagacc tcgctgtcg tggcagaac ggtagtggg 
      cagccgtca acgtcgaac tcgtccact ttggagaaa 
      aactccacg ccttgtagg cccaagcgg acgcggaag 
      cacgaccgg aataattgt ctctacaag aaaaacagt 
      caggagatg aagaagagt gacgatttt ccctccaga 
      cgggaggag caagttgtt acgttacct gtgagtaca 
      attcatggc ttgaacggc cggttgcac cacctggag 
      cagaggtgc aggcagctg cagatggtg ctgaagcag 
      cagcatttt tacacggaa caggccttg caacagtgt 
      cggcagcat cacaagagt gagaagacc gaattgcag 
      tgcattttg cgcagtcat caagagaag ttggaccaa 
      caagtgagg atctgcaac gatcagttg gagagactc 
      gccaggtca gatatgtta gtcaaggag ttgtatgtg 
      gagaattcg tatctgatt gcaaaagta caaagactt 
      gagcaacag tgccatatc ttggcacag tgtaactcg 
      aatagtagt tcggtgtag ttggagcct atttttgcc 
      taattgtag cgatagttg actattttt aaagactaa 
      atatttaat tttgtgtga gtttttttt tgtatattc 
      tgattgatt gttaaatta cctgactag agaattgta 
      agagtaata atgttatgg gttgtttat tcttttata 
      tgatgtact tttgttaca tatcattta tttttactt 
      atatccaaa gaatttaac acgataagt atctagtta 
      ctgttacta ttaactact aatttttaa agtgtcata 
      ttattgata aataaagta acgtcaaac at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY