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Gene information
General information
TC012418 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7042199..7049164
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012418
    iBeetle screen iB_05238

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs tibiotarsus potentially swollen - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs podomer potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 62.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Adult morphology
    elytra wing veins slightly irregular - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012418 RA
      MAEKKPSPL DLNSPSFNP NMYLDKLFK ESNLKQIMD 
      HESEIVKDT QTLHSDMQT LVYENYNKF ISATDTIKK 
      MKVDFKKME NEMDLLASN MSSITSFSD QINSTLQDT 
      RSQISKLSG VHTLLQRLQ FLFKLPTSL KTRMEEKRY 
      IEAVQDYLH AQRVLEQYG NLPSLQGIK TDCETILIE 
      LKKELRKHF SNPQASAKE LTESVDLLL QLNEPAKEL 
      CSEFLICAE KRLSDQLVM LKDQSEQRD ISEFIDLGC 
      SGFLSDLCL VVASFDDMF INRNNLDNI ENSDIFENF 
      AATELNNFV KQNMAKYFD LVQNKVDLE QDVGDTAVL 
      VQALDRFYK RIESNTLCR DVDFSALAT EIVINAGRK 
      QCKAHLQIL KMHFADCLT KVRQNLTTP KLISQDDSS 
      KNLNELLST LIMSIVEKI KGVLQDLVV FIQPEVNFA 
      QKPQFKDSF CVDNVREGL IVCFMHHLT ATARSYCAI 
      VASDLKVPP TVLLLLSKL CLDFRNGSV SFVLSQTDE 
      LFQINPKQS AALTNEGEI NMTMQESAQ ELLNYYVRI 
      QGLNVSQML RKSVETRDW LHTIEPRTV RAVMKRVVE 
      DISAIDATV ALLYEDQGN NTEHSSDSS RKTHSISVS 
      RHQYRSNWS SLTPNHLDS TLVSNMHKL FSERIEIFS 
      TVEFNKISI LTGIIKISL KTFMECVRL KTFSKFGLQ 
      QIQVDTHYL QLYLWRFVA DENLVHFLL DEILGSAVH 
      RCLEPILME PSVVDIICE RG
    CDS sequence
    • >TC012418 RA
      atggcggag aaaaagccc agccccttg gacctgaac 
      agtcccagc ttcaacccc aacatgtat ttggataaa 
      ttattcaaa gagtccaac ttgaagcaa ataatggac 
      cacgagagc gaaatcgtc aaagacaca caaaccttg 
      cattccgac atgcaaacc ctagtttac gaaaattat 
      aacaaattt atttcggct acagacacc ataaaaaag 
      atgaaagtc gatttcaaa aaaatggaa aacgaaatg 
      gacctcctc gcctcaaac atgtcgtca attacgtcg 
      ttttccgac caaattaac tcgactctt caggacaca 
      aggagccaa attagtaaa ttgtcgggg gtgcacacg 
      cttttgcag cgcctccag tttttattc aaactgcca 
      acaagcttg aaaactcga atggaggaa aagcgatac 
      attgaggcg gttcaggac tatttgcac gcccagcgt 
      gtcttggaa cagtacggt aacttgcca tccttgcaa 
      ggcatcaaa accgactgt gaaaccatc cttattgag 
      ctcaaaaaa gaactgagg aagcacttc agtaaccct 
      caagcctcg gccaaggaa ctgaccgaa agtgtggat 
      ttgctcctg caactgaac gagccggcc aaagagttg 
      tgttctgaa tttttgatt tgtgccgaa aaacgtctc 
      agtgaccaa ctagtcatg ctgaaggac caaagcgag 
      caacgggac atttccgaa tttattgat ttgggttgt 
      tcgggattt ttgagcgat ttgtgtctg gttgtggct 
      tcctttgac gacatgttt atcaatcgc aacaatttg 
      gataatatc gaaaatagt gatattttc gaaaatttt 
      gctgcaact gaactcaac aactttgtt aaacaaaac 
      atggcgaag tatttcgat ttggtgcaa aataaagtc 
      gatttggag caggatgtt ggagatacg gctgtttta 
      gttcaagcc ttggaccgg ttttataaa aggattgaa 
      agtaacaca ctgtgcagg gatgtggat ttttcggct 
      cttgcgacc gagattgtg ataaatgca ggtcggaaa 
      cagtgcaaa gcacatttg caaatcctt aaaatgcac 
      tttgctgat tgtttgacc aaagtgagg caaaacttg 
      acgactcca aagctgata agtcaagac gactcatct 
      aaaaatctg aatgagctt ctcagcaca cttataatg 
      tcaatagtt gagaaaatt aagggcgtt ttgcaggac 
      cttgtggtg ttcattcaa ccagaggtg aatttcgcc 
      caaaaaccc caatttaaa gacagtttt tgcgtggat 
      aacgtccga gaggggctc atagtatgt ttcatgcac 
      cacttgact gcaactgcg cgcagttac tgcgctatt 
      gtggcttcc gacttgaag gtgccccca actgtcctt 
      ctcttgttg tcaaaatta tgcctcgat tttcgcaac 
      ggaagcgtc agtttcgtg ctctctcaa acggatgaa 
      ctgtttcaa atcaacccc aaacaaagc gctgctttg 
      actaacgag ggcgagatt aatatgacg atgcaagag 
      tcggcgcaa gaacttttg aattattac gtgaggata 
      caaggactt aacgtttca cagatgttg cgcaaaagc 
      gtcgaaacg agggactgg ctgcacaca atagagccg 
      cggactgtg agggctgtg atgaaacga gtcgttgaa 
      gatatttca gctattgac gccactgtt gcccttttg 
      tacgaagac cagggtaat aatacggaa cacagtagc 
      gattccagc cgtaaaacg cacagtatt tcggtctca 
      agacaccag tacaggtcg aattggtca agcttgacc 
      cctaatcac ttggattcg actctagtc tcgaatatg 
      cacaagttg ttcagcgaa aggattgaa attttctca 
      actgttgag tttaataaa atttcgatt ctcactggg 
      attattaag attagtctc aagacgttc atggagtgc 
      gtacgtctg aagactttt agtaaattt ggcctccaa 
      caaattcaa gtggacacg cattacttg cagttgtat 
      ttgtggcga tttgttgcg gatgaaaac ttggttcat 
      ttcctcctg gatgagatt ttgggatca gcagtccac 
      aggtgcctg gaaccgatt ttaatggaa ccaagcgtt 
      gtggacatc atatgtgaa aggggttag
    mRNA sequence
    • >TC012418 RA
      ttggtgaca acaatacaa aaaatgttt tttgagttc 
      tttttcaat taagtgttc aaaaaccca aaaatggcg 
      gagaaaaag cccagcccc ttggacctg aacagtccc 
      agcttcaac cccaacatg tatttggat aaattattc 
      aaagagtcc aacttgaag caaataatg gaccacgag 
      agcgaaatc gtcaaagac acacaaacc ttgcattcc 
      gacatgcaa accctagtt tacgaaaat tataacaaa 
      tttatttcg gctacagac accataaaa aagatgaaa 
      gtcgatttc aaaaaaatg gaaaacgaa atggacctc 
      ctcgcctca aacatgtcg tcaattacg tcgttttcc 
      gaccaaatt aactcgact cttcaggac acaaggagc 
      caaattagt aaattgtcg ggggtgcac acgcttttg 
      cagcgcctc cagttttta ttcaaactg ccaacaagc 
      ttgaaaact cgaatggag gaaaagcga tacattgag 
      gcggttcag gactatttg cacgcccag cgtgtcttg 
      gaacagtac ggtaacttg ccatccttg caaggcatc 
      aaaaccgac tgtgaaacc atccttatt gagctcaaa 
      aaagaactg aggaagcac ttcagtaac cctcaagcc 
      tcggccaag gaactgacc gaaagtgtg gatttgctc 
      ctgcaactg aacgagccg gccaaagag ttgtgttct 
      gaatttttg atttgtgcc gaaaaacgt ctcagtgac 
      caactagtc atgctgaag gaccaaagc gagcaacgg 
      gacatttcc gaatttatt gatttgggt tgttcggga 
      tttttgagc gatttgtgt ctggttgtg gcttccttt 
      gacgacatg tttatcaat cgcaacaat ttggataat 
      atcgaaaat agtgatatt ttcgaaaat tttgctgca 
      actgaactc aacaacttt gttaaacaa aacatggcg 
      aagtatttc gatttggtg caaaataaa gtcgatttg 
      gagcaggat gttggagat acggctgtt ttagttcaa 
      gccttggac cggttttat aaaaggatt gaaagtaac 
      acactgtgc agggatgtg gatttttcg gctcttgcg 
      accgagatt gtgataaat gcaggtcgg aaacagtgc 
      aaagcacat ttgcaaatc cttaaaatg cactttgct 
      gattgtttg accaaagtg aggcaaaac ttgacgact 
      ccaaagctg ataagtcaa gacgactca tctaaaaat 
      ctgaatgag cttctcagc acacttata atgtcaata 
      gttgagaaa attaagggc gttttgcag gaccttgtg 
      gtgttcatt caaccagag gtgaatttc gcccaaaaa 
      ccccaattt aaagacagt ttttgcgtg gataacgtc 
      cgagagggg ctcatagta tgtttcatg caccacttg 
      actgcaact gcgcgcagt tactgcgct attgtggct 
      tccgacttg aaggtgccc ccaactgtc cttctcttg 
      ttgtcaaaa ttatgcctc gattttcgc aacggaagc 
      gtcagtttc gtgctctct caaacggat gaactgttt 
      caaatcaac cccaaacaa agcgctgct ttgactaac 
      gagggcgag attaatatg acgatgcaa gagtcggcg 
      caagaactt ttgaattat tacgtgagg atacaagga 
      cttaacgtt tcacagatg ttgcgcaaa agcgtcgaa 
      acgagggac tggctgcac acaatagag ccgcggact 
      gtgagggct gtgatgaaa cgagtcgtt gaagatatt 
      tcagctatt gacgccact gttgccctt ttgtacgaa 
      gaccagggt aataatacg gaacacagt agcgattcc 
      agccgtaaa acgcacagt atttcggtc tcaagacac 
      cagtacagg tcgaattgg tcaagcttg acccctaat 
      cacttggat tcgactcta gtctcgaat atgcacaag 
      ttgttcagc gaaaggatt gaaattttc tcaactgtt 
      gagtttaat aaaatttcg attctcact gggattatt 
      aagattagt ctcaagacg ttcatggag tgcgtacgt 
      ctgaagact tttagtaaa tttggcctc caacaaatt 
      caagtggac acgcattac ttgcagttg tatttgtgg 
      cgatttgtt gcggatgaa aacttggtt catttcctc 
      ctggatgag attttggga tcagcagtc cacaggtgc 
      ctggaaccg attttaatg gaaccaagc gttgtggac 
      atcatatgt gaaaggggt taggtcatg cagcttgca 
      taagttaca aatatttat tattctttt gtattatct 
      tgtttaaat aaaaaggag aaatttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY