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Gene information
General information
TC012411 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6787993..6789434
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012411
    iBeetle screen iB_05236

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4
    vitellogenic egg chamber mostly not present - Number of animals showing the phenotype: 4

    tropharium structure irregular - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 37.5% (includes death as larva, prepupa, pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Adult morphology
    head dorsal posteriorsurface irregular - Number of animals showing the phenotype: 7

    Comment: head with distinct depression

    pronotum surface irregular - Number of animals showing the phenotype: 7

    Comment: pronotum with several symmetric, distinct depressions

    Fertility
    egg production: number no/almost no eggs -
    egg chamber not present - Number of animals showing the phenotype: 1

    tropharium shape irregular - Number of animals showing the phenotype: 2
    tropharium structure irregular - Number of animals showing the phenotype: 2
    Comment: big vacuole-like structures in the posterior part, no nuclei visible in there
    egg chamber number decreased - Number of animals showing the phenotype: 1

    previtellogenic egg chamber mostly not present - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012411 RA
      MLKSEPSTM SGKTVIKGN PSQYVKLNV GGCLHYTTI 
      GTLTKHDTM LRAMFSGRM EVLSDSDGW ILIDRCGKH 
      FGAILNFLR DGSVSLPES SKEIAELFA EAKYYCIQE 
      LAESCTQAL AKKERNEHD PLCKVPLIT SQKEEELLI 
      SSTTKPAIK LLINRHNNK YSYTTTSDD NLLKNIELF 
      DKLSLWYSK RVLFLKDVI STSEICCWV FYGHGKKVS 
      EVCCTSIVY ATDKKHTKV ECPEARIYE ETLNILLYE 
      NRNAPDQEL MQVTSTRGA VSGMSSYTS DEEEERSGL 
      DRLRSNKSN NQA
    CDS sequence
    • >TC012411 RA
      atgttgaaa tcggaaccc agcaccatg tcgggcaag 
      actgtgatc aaaggcaac ccctcgcag tacgtgaag 
      ttgaacgtg gggggctgt cttcactac accacaatt 
      gggacgctg accaagcac gacacgatg ctgcgggcg 
      atgttcagc ggcaggatg gaagtattg tcggattcg 
      gacggttgg atcctgatt gaccgctgt gggaagcac 
      tttggggcc attttgaac ttcctgagg gacggcagc 
      gtgtccctc cctgagagc agcaaagaa atcgccgaa 
      ctgttcgcc gaggccaag tattactgc atccaagag 
      ctggccgag tcgtgcact caggccctg gccaagaag 
      gaacggaac gaacacgac cccctgtgc aaggtcccg 
      ctcattacc tcacaaaag gaggaggag ctcctgata 
      agctcaaca acaaagccg gcgattaag ctgctgata 
      aaccgccac aacaacaaa tattcctac accacgact 
      tccgacgac aatttgttg aaaaacatc gaactgttc 
      gataagctg tcgctgtgg tacagcaag cgcgttctg 
      ttcctcaaa gacgtgata agcaccagc gagatctgc 
      tgctgggtc ttctacggc cacggcaag aaggtctcg 
      gaagtgtgc tgcacgtcg attgtgtac gcgactgat 
      aagaagcac acgaaagtc gagtgtccc gaggctagg 
      atttacgaa gaaacgctt aatattttg ctttacgag 
      aacaggaac gcccccgac caggagttg atgcaggtt 
      acgtcgact cggggggct gtctctggc atgtcttcc 
      tacacgagc gacgaggag gaggagcgc tccgggttg 
      gaccggctc aggtcgaat aagtccaat aatcaagcc 
      taa
    mRNA sequence
    • >TC012411 RA
      aaacatggc acccatcag taattaaag tggttttgt 
      taattaaat aacgtgcaa attgatgaa aataacggt 
      ttaatgttg aaatcggaa cccagcacc atgtcgggc 
      aagactgtg atcaaaggc aacccctcg cagtacgtg 
      aagttgaac gtggggggc tgtcttcac tacaccaca 
      attgggacg ctgaccaag cacgacacg atgctgcgg 
      gcgatgttc agcggcagg atggaagta ttgtcggat 
      tcggacggt tggatcctg attgaccgc tgtgggaag 
      cactttggg gccattttg aacttcctg agggacggc 
      agcgtgtcc ctccctgag agcagcaaa gaaatcgcc 
      gaactgttc gccgaggcc aagtattac tgcatccaa 
      gagctggcc gagtcgtgc actcaggcc ctggccaag 
      aaggaacgg aacgaacac gaccccctg tgcaaggtc 
      ccgctcatt acctcacaa aaggaggag gagctcctg 
      ataagctca acaacaaag ccggcgatt aagctgctg 
      ataaaccgc cacaacaac aaatattcc tacaccacg 
      acttccgac gacaatttg ttgaaaaac atcgaactg 
      ttcgataag ctgtcgctg tggtacagc aagcgcgtt 
      ctgttcctc aaagacgtg ataagcacc agcgagatc 
      tgctgctgg gtcttctac ggccacggc aagaaggtc 
      tcggaagtg tgctgcacg tcgattgtg tacgcgact 
      gataagaag cacacgaaa gtcgagtgt cccgaggct 
      aggatttac gaagaaacg cttaatatt ttgctttac 
      gagaacagg aacgccccc gaccaggag ttgatgcag 
      gttacgtcg actcggggg gctgtctct ggcatgtct 
      tcctacacg agcgacgag gaggaggag cgctccggg 
      ttggaccgg ctcaggtcg aataagtcc aataatcaa 
      gcctaacta ttgtcttat ttgtttaaa ttggtcggg 
      tttgggcca cacctgtgc taggtttta cgttggtag 
      gactgtacc aacctgatc tcgagattc caaagcggt 
      gactgattt tttcgtaaa actgtggcc taaatgcgg 
      tgactaatt taactcaag ttcaagctt gaacagtat 
      ttgcgtaat agagttatt ttcataatt gatacactt 
      tttaaggct tgaattgtg gagaacttt tagttcttc 
      gtaacccga acgtacttt tatttttca agcttaaaa 
      acggtgtcg attttgaag tatttttgc gattacccg 
      agattttag cagctgttg agcccctag atgaagagt 
      tgtgatatt ttagagcag attaggagg caattcagt 
      gttttaaat taaagaatc aagtatgta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY