Status
Please wait ...
Gene information
General information
TC034317
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012394,TC012395
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6135130..6171975
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034317
    iBeetle screen iB_05235

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 4

    tropharium structure irregular - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    Pupal morphology
    wings potentially blistered - Number of animals showing the phenotype: 2
    Comment: animals are dead, dried out and therefore not fully analyzable
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034317 RA
      MSGSHMNSE QEHKSPRES GEDSEDESE ILEESPCGR 
      WLKRREEVE QRDVPGIDC AYLAMDTEE GVEVVWNEV 
      QFSERKNFK NQEEKIQLI FENLTQLEH PNIVKFHRY 
      WTDTHNDKP RVIFITEYM SSGSLKQFL KRTKRNVRR 
      LPLPAWRRW CTQILSALS YLHSCSPPI IHGNLTCDT 
      IFIQHNGLV KIGSVAPDS INHHVKTCQ DDFKNMHFI 
      APEHPSTMG PPMDIYSFG MCALEMAAL EIVGNGDSG 
      NKVTEENIK KTIDSLEDE QQKDFIKKC LQKSPNDRP 
      TAKELLFHP LLFEVHSLK LLAAHCLVK RSANISETI 
      TDELMQRWY GPDMIVAEI RRKNGPPVQ MKMSDVPVT 
      EKLEKFVED VKNGIYPLT AFGAKQPPP ARSRAISPE 
      MAESVKSVT PEPMDVENR KIVNMMCDI KPKEESCDL 
      LLTIVLRMD DKMNRHLTS CITQTDSPK ALAQELVHY 
      GFINEDDRD KIATLIGET LKDCCSGRS SPSRIFQPL 
      SHLPPPTQP AAALVNATL PLAATAVVN STPTQVPMH 
      STS
    CDS sequence
    • >TC034317 RA
      atgtcgggg agtcatatg aatagcgag caggagcac 
      aagtcgcca agggaaagc ggcgaggac tcggaagac 
      gaaagcgaa atcctggag gagagcccc tgcggcaga 
      tggcttaag cggcgagag gaggtggaa caaagagat 
      gtgcctgga atcgactgc gcctatctg gccatggac 
      acagaagaa ggagtcgaa gtcgtatgg aacgaagtc 
      cagttttcg gaacgtaaa aattttaag aaccaagag 
      gaaaaaatc caactcatt tttgaaaat ctcacgcaa 
      ctcgagcac cctaacatt gtcaaattc catcggtat 
      tggaccgat acacacaac gacaagcct cgagtcata 
      ttcattacg gaatacatg tcctcaggt tccttgaaa 
      cagttcctc aagcgtacg aaacgcaac gtgagaagg 
      ctgccactc cctgcgtgg aggagatgg tgtacacag 
      atcctctcc gctttaagt tacttgcat tcctgttct 
      ccgccaatc atccacggc aatctgacc tgtgatacc 
      atatttata cagcacaat ggcctcgtc aagatcgga 
      tcagtggct cctgattcc ataaaccat cacgttaaa 
      acatgccag gacgatttc aaaaatatg catttcatt 
      gcaccagaa catcctagc acaatgggc cccccgatg 
      gacatctac tcgttcggc atgtgcgcg ctggaaatg 
      gccgctctc gaaatcgtg ggcaacggc gactccggc 
      aacaaagta acggaggag aacatcaag aaaacgatc 
      gattcgctc gaggacgag cagcagaag gatttcatc 
      aagaagtgt ctccagaag agccccaac gaccggccg 
      acagccaag gagctcctc ttccacccc ctcctgttc 
      gaagtgcac agtcttaag ctcctggcg gcccattgt 
      ttggtcaag agatctgca aacatatct gaaacgata 
      acagatgag ttgatgcag aggtggtac gggcctgat 
      atgatcgtg gccgagatc cggcgcaag aatggaccc 
      ccggttcag atgaaaatg tccgacgtt cccgtgacg 
      gagaagttg gagaagttc gtcgaggac gtgaaaaac 
      ggcatttat cctttaacg gccttcggt gctaagcaa 
      ccccctcca gcccgatcg agggcgata tcaccggaa 
      atggcggaa tcggtcaag tccgtcacg ccggaaccg 
      atggatgta gaaaatagg aaaattgtc aatatgatg 
      tgcgacatc aaacccaaa gaagagagc tgtgattta 
      ttgttgacg attgtactc cgtatggac gataaaatg 
      aatcgccat ttgactagt tgtattacg caaacggat 
      tctccgaaa gctttagct caagagttg gtacattat 
      ggtttcata aatgaagac gatcgagac aagattgcc 
      acccttata ggagagacg ttaaaggac tgttgttcc 
      ggccgtagt agtccgagt cgaattttt cagccctta 
      tcgcatctg ccccctcct acccaaccg gcggcggcc 
      ttggtgaat gcaacgtta ccgttggcg gcgacggcg 
      gtggtcaat tccacacct actcaagtg cccatgcac 
      tccactagt tag
    mRNA sequence
    • >TC034317 RA
      agccaagtc aaaatacgc gaaccccag aagtgggga 
      agtggcgca gtctcagtg cggctccca ataagcggt 
      caaaatcag atgagtctc cgtcggtgt aattcggta 
      tcggtaaag ctctgagcc agttgaaaa ttcactctg 
      aacaaactg tcccgtgcc cgtgattgt cgcgtcgcc 
      ccggtggaa gccccactg tcgatcccg cctccgttc 
      ggtggttgt tgtcaccgc acgctccag ctccagttc 
      aataacact gcagacgag ctctttgtg ccgttaggt 
      gtcttcgag tgtgtgtcg gttgtaaaa tgctaggtt 
      agggtctgg cgaagtcgg ccagagtgt ggtgtctca 
      gacgatttg ttcgatggt gaaatctgt ggattatgc 
      gctgctgat ggaatacga ctgttggtg cattgccct 
      gtcgacaaa cgcacaggt tgtgcggtt tcgggatac 
      ttcacctca actataatg tcggggagt catatgaat 
      agcgagcag gagcacaag tcgccaagg gaaagcggc 
      gaggactcg gaagacgaa agcgaaatc ctggaggag 
      agcccctgc ggcagatgg cttaagcgg cgagaggag 
      gtggaacaa agagatgtg cctggaatc gactgcgcc 
      tatctggcc atggacaca gaagaagga gtcgaagtc 
      gtatggaac gaagtccag ttttcggaa cgtaaaaat 
      tttaagaac caagaggaa aaaatccaa ctcattttt 
      gaaaatctc acgcaactc gagcaccct aacattgtc 
      aaattccat cggtattgg accgataca cacaacgac 
      aagcctcga gtcatattc attacggaa tacatgtcc 
      tcaggttcc ttgaaacag ttcctcaag cgtacgaaa 
      cgcaacgtg agaaggctg ccactccct gcgtggagg 
      agatggtgt acacagatc ctctccgct ttaagttac 
      ttgcattcc tgttctccg ccaatcatc cacggcaat 
      ctgacctgt gataccata tttatacag cacaatggc 
      ctcgtcaag atcggatca gtggctcct gattccata 
      aaccatcac gttaaaaca tgccaggac gatttcaaa 
      aatatgcat ttcattgca ccagaacat cctagcaca 
      atgggcccc ccgatggac atctactcg ttcggcatg 
      tgcgcgctg gaaatggcc gctctcgaa atcgtgggc 
      aacggcgac tccggcaac aaagtaacg gaggagaac 
      atcaagaaa acgatcgat tcgctcgag gacgagcag 
      cagaaggat ttcatcaag aagtgtctc cagaagagc 
      cccaacgac cggccgaca gccaaggag ctcctcttc 
      caccccctc ctgttcgaa gtgcacagt cttaagctc 
      ctggcggcc cattgtttg gtcaagaga tctgcaaac 
      atatctgaa acgataaca gatgagttg atgcagagg 
      tggtacggg cctgatatg atcgtggcc gagatccgg 
      cgcaagaat ggacccccg gttcagatg aaaatgtcc 
      gacgttccc gtgacggag aagttggag aagttcgtc 
      gaggacgtg aaaaacggc atttatcct ttaacggcc 
      ttcggtgct aagcaaccc cctccagcc cgatcgagg 
      gcgatatca ccggaaatg gcggaatcg gtcaagtcc 
      gtcacgccg gaaccgatg gatgtagaa aataggaaa 
      attgtcaat atgatgtgc gacatcaaa cccaaagaa 
      gagagctgt gatttattg ttgacgatt gtactccgt 
      atggacgat aaaatgaat cgccatttg actagttgt 
      attacgcaa acggattct ccgaaagct ttagctcaa 
      gagttggta cattatggt ttcataaat gaagacgat 
      cgagacaag attgccacc cttatagga gagacgtta 
      aaggactgt tgttccggc cgtagtagt ccgagtcga 
      atttttcag cccttatcg catctgccc cctcctacc 
      caaccggcg gcggccttg gtgaatgca acgttaccg 
      ttggcggcg acggcggtg gtcaattcc acacctact 
      caagtgccc atgcactcc actagttag taaaacata 
      gatactgcc aatgcactt tcgtatttc taagatgtc 
      aaagtaaac gttatttat cataaacac agtgtctgg 
      actaactag actatcgtg gaaaaaatg cagggagtt 
      aataataaa aatcgggat tccgatcag tcgttatcg 
      gcgtccaga tgcgtgata aaccgtaga taagcttac 
      taggccgcc tgcattttt ccgagctag gttcatgcc 
      ttatttggg ttagtctac gtcatggta gagttagaa 
      agaagaaga ctgtaaaat gctggacat tacactagt 
      catgtgacc acgtgttaa tctgtgatc agagcttca 
      atgacataa acgctggac tgtttaatc atagatttc 
      atattgtat gtgatctgt ttatgataa ttttgtaac 
      tcctaggcc gcgtcgcaa gaatctgtt gaatcgttt 
      cgtttcgtt tctgtcaat actcgtcgc aaatgcatt 
      attgtttgc cccttgaga ctgatgatg tactaatta 
      aggagaaat tgtgaatag tggtagtcg tgcaggaca 
      ggaccaatg tggaaggtt agtgtcgtt gggaactag 
      catttacgt cagcttttg tttcgtcaa cgaattttt 
      tcgggccta aattctgtt gtttgcgac tgacgtgaa 
      tggcgagtt gggtcagtg gagggattg taaataggg 
      taattctaa caagtgtat gaaattgta gatattgga 
      ttttttatt gaggaaatt agagtaagg attatttat 
      ttctgttag aggtcccgt gtgataatt cgctcaaag 
      ttctggatg atggctaac ctgtgtagg ttgatagtc 
      ggttcgctt gcccatccg attccagta cagccgcag 
      cagtctttc cgttaacta aaccaatgt tcacactca 
      cagcttctt caccgtaac ctcttgaga aacacattc 
      agattactg ttcttccct agagaccga acacacgtg 
      cagagtttt tatttgtgg acatccata cgtatttcg 
      ctgttagtg atattatag actatgatt ttttatact 
      tatttattt ctcatttgt tacccatac ccatcggtt 
      cgcattgcc atcatgaat tagcttgtt tatgattta 
      ggttagtca tgcctctgc atgttgttc attattatt 
      gctcacatt gagcaagtg tatatcaaa ttctatatt 
      gaatgctac ggagacttt gcatttcaa gctcccgcc 
      aaacacctc agcgctggc tctcgagtg ctcgccact 
      ccctcaatt acataattt catttaagt tgtgtttat 
      ttgtattgt aatatcacc attctttat tgtatacct 
      tatttaaat aaagatgca atacggac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY