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Gene information
General information
TC012393 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6123660..6132350
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012393
    iBeetle screen iB_05234

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012393 RA
      MTRTKEKIL HDNKPLNST FYHHKSHQT TLLKGLNSL 
      WEKGELLDV TLIIEGQLF KAHKAVLSA CSDYFRAMF 
      TNNMLESRQ DEICLNGIT AVGFHQILE YAYTSRIML 
      NLGNIQDVL EAASHIQME PVIQACSKY LLSQIDIDN 
      CIDIATIAE IYSLEKLRL MVYRFMSEH LLEFSNSKE 
      FYRLTPQQL ENLLALDFP VDCSEADVL RIVLAWFFH 
      VDTSKRDLR ISYAMRIIH YIHFKEISR RKLDSILNK 
      IFQDRNCEW ELYKVILSE AYIQTNSNK ITSNSNLLN 
      SRGMELAVL KIGGFGISG ITNEITYCF SSERKWKHL 
      TTIPHLEQC NFGTAVLNN ELYIVGGCF NQSLQENIH 
      PFGFKYSPR YDKWSTMSP MKIERCRFS LNVLSNMLY 
      AVGGASEVD EYGTSTCEC YDPVLDMWY MIAPLPAHI 
      TQHAGASYE HNLSLKLFV SGGMDRDSI QSAMYCYAV 
      SEDRWQPCA PLLRARADH VMLSIGDFL YVCGGWTEG 
      PEGRSRVLV DTIDVYDIK NDRWEVVTT VPTPRYHAG 
      IVCVEHKIY FIGGFYSDD MFDKSTAAI EFYDIESDS 
      WTTEEKYPQ DIWEHTCAS LFIPKWRDD MQDMQVIAK 
      TESTV
    CDS sequence
    • >TC012393 RA
      atgacacga acgaaggaa aaaatcctc cacgacaac 
      aaacccctg aactcgact ttctaccac cacaagtcg 
      caccaaacc acgctgctc aaagggctg aactcgctg 
      tgggaaaag ggcgagctg ctggacgtc accctgatc 
      atcgaaggg cagcttttc aaggcccac aaggctgtt 
      ctatcggca tgcagcgat tatttccgt gccatgttc 
      acaaataac atgctagag tccagacag gatgagatc 
      tgtctcaat ggaatcacc gctgtcggg ttccatcaa 
      atactcgaa tacgcttac acgtccagg attatgctt 
      aatttaggg aatatacag gatgttctg gaggcggcg 
      tcgcatatt caaatggaa ccggttatc caggcctgc 
      tccaagtac ctactctcg cagattgac atcgacaat 
      tgcattgat attgcgaca atagccgag atttactcg 
      ttggaaaaa ttaagattg atggtttat agatttatg 
      agcgagcat ttgttggag ttttcaaat tctaaagag 
      ttttataga ctgacgccg caacagttg gaaaatctt 
      ctagcgctt gattttccg gttgattgt tccgaagcc 
      gacgttttg aggatcgtt ttggcctgg tttttccac 
      gtcgatacc tccaaaagg gatctacga atttcctat 
      gccatgcga ataattcat tacattcac ttcaaagaa 
      atttcccgt cgaaaattg gatagtatc ttgaataag 
      atctttcag gaccgcaac tgcgaatgg gaactttac 
      aaagtgatt ttatcagaa gcttacatt caaactaac 
      tcaaataaa attacatcc aactcaaat ttactcaac 
      tcgcgtggg atggaatta gcggtatta aaaatcggg 
      ggcttcgga atcagcgga ataaccaac gaaatcact 
      tattgcttc tcaagcgaa agaaaatgg aagcatttg 
      accacaatc ccccacctg gagcaatgc aacttcggc 
      acggcggtc ctcaacaac gagctctac atcgtcggg 
      ggctgcttc aaccagtcc ctccaggag aacatccac 
      cccttcggc ttcaagtac agcccccgg tacgacaag 
      tggtccacc atgtccccc atgaagatc gagcgatgc 
      cgcttctcg ctgaacgtc ctcagcaac atgctgtac 
      gcagtgggg ggcgccagc gaggtggac gagtacggc 
      accagcacg tgcgagtgc tacgacccc gtgctggac 
      atgtggtac atgatagcc cccttgccg gcgcacatc 
      acgcagcac gcgggcgcg agctacgag cacaacctc 
      agtttgaag ctcttcgtc agcgggggc atggaccgc 
      gactccatc cagtccgcc atgtactgc tacgcggtg 
      agcgaggac aggtggcag ccctgcgcc cccctgttg 
      cgggcgcgg gccgaccac gtcatgctc tccatcggg 
      gacttcctg tacgtgtgc gggggctgg accgagggc 
      cccgagggg cggtcgagg gttctggtc gacacgatt 
      gacgtttat gacatcaag aatgatcgc tgggaggtc 
      gtcaccacc gtgccaaca ccccggtat catgcgggg 
      attgtgtgt gtggagcat aagatttac ttcattggg 
      gggttttat agcgacgat atgtttgat aaaagtaca 
      gcggccata gagttttac gatattgag agcgattcc 
      tggacgacg gaggagaag tatccgcag gatatatgg 
      gaacatacg tgtgcctcg ttgtttatc cccaagtgg 
      agggacgat atgcaggac atgcaggtc attgccaaa 
      acggaaagc acggtttaa
    mRNA sequence
    • >TC012393 RA
      tttattgca tcaaccgtg gacgtagaa taatcaatc 
      cgtccattt cttctatct catccgttc attgttaca 
      gaacgttcc aaaacggat acagatggc ctacagatc 
      tcctctata ttttctagt taaattcca tataaagtg 
      tgtccagaa ataaaaatg tgaaaggca aagtgtgct 
      tgatttact tatcgacta ttcctgata tggtagctt 
      gccataatg acacgaacg aaggaaaaa atcctccac 
      gacaacaaa cccctgaac tcgactttc taccaccac 
      aagtcgcac caaaccacg ctgctcaaa gggctgaac 
      tcgctgtgg gaaaagggc gagctgctg gacgtcacc 
      ctgatcatc gaagggcag cttttcaag gcccacaag 
      gctgttcta tcggcatgc agcgattat ttccgtgcc 
      atgttcaca aataacatg ctagagtcc agacaggat 
      gagatctgt ctcaatgga atcaccgct gtcgggttc 
      catcaaata ctcgaatac gcttacacg tccaggatt 
      atgcttaat ttagggaat atacaggat gttctggag 
      gcggcgtcg catattcaa atggaaccg gttatccag 
      gcctgctcc aagtaccta ctctcgcag attgacatc 
      gacaattgc attgatatt gcgacaata gccgagatt 
      tactcgttg gaaaaatta agattgatg gtttataga 
      tttatgagc gagcatttg ttggagttt tcaaattct 
      aaagagttt tatagactg acgccgcaa cagttggaa 
      aatcttcta gcgcttgat tttccggtt gattgttcc 
      gaagccgac gttttgagg atcgttttg gcctggttt 
      ttccacgtc gatacctcc aaaagggat ctacgaatt 
      tcctatgcc atgcgaata attcattac attcacttc 
      aaagaaatt tcccgtcga aaattggat agtatcttg 
      aataagatc tttcaggac cgcaactgc gaatgggaa 
      ctttacaaa gtgatttta tcagaagct tacattcaa 
      actaactca aataaaatt acatccaac tcaaattta 
      ctcaactcg cgtgggatg gaattagcg gtattaaaa 
      atcgggggc ttcggaatc agcggaata accaacgaa 
      atcacttat tgcttctca agcgaaaga aaatggaag 
      catttgacc acaatcccc cacctggag caatgcaac 
      ttcggcacg gcggtcctc aacaacgag ctctacatc 
      gtcgggggc tgcttcaac cagtccctc caggagaac 
      atccacccc ttcggcttc aagtacagc ccccggtac 
      gacaagtgg tccaccatg tcccccatg aagatcgag 
      cgatgccgc ttctcgctg aacgtcctc agcaacatg 
      ctgtacgca gtggggggc gccagcgag gtggacgag 
      tacggcacc agcacgtgc gagtgctac gaccccgtg 
      ctggacatg tggtacatg atagccccc ttgccggcg 
      cacatcacg cagcacgcg ggcgcgagc tacgagcac 
      aacctcagt ttgaagctc ttcgtcagc gggggcatg 
      gaccgcgac tccatccag tccgccatg tactgctac 
      gcggtgagc gaggacagg tggcagccc tgcgccccc 
      ctgttgcgg gcgcgggcc gaccacgtc atgctctcc 
      atcggggac ttcctgtac gtgtgcggg ggctggacc 
      gagggcccc gaggggcgg tcgagggtt ctggtcgac 
      acgattgac gtttatgac atcaagaat gatcgctgg 
      gaggtcgtc accaccgtg ccaacaccc cggtatcat 
      gcggggatt gtgtgtgtg gagcataag atttacttc 
      attgggggg ttttatagc gacgatatg tttgataaa 
      agtacagcg gccatagag ttttacgat attgagagc 
      gattcctgg acgacggag gagaagtat ccgcaggat 
      atatgggaa catacgtgt gcctcgttg tttatcccc 
      aagtggagg gacgatatg caggacatg caggtcatt 
      gccaaaacg gaaagcacg gtttaagtc tttggattt 
      tcttatttt tatatgttt ggattttga gataatata 
      tttattttg taaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY