Status
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Gene information
General information
TC012392 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6070717..6088081
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012392
    iBeetle screen iB_05233

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    egg chamber slightly number decreased - Number of animals showing the phenotype: 3

    Comment: Very late vitellogenic egg chambers are mostly not present. But in general the ovaries look quite normal.
    egg chamber mostly not present - Number of animals showing the phenotype: 1

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    Comment: below the larval cuticle pupal (gin traps) and adult (abdominal sternites) structures are visible

    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012392 RA
      MTKSDERHT CFLLLEDGT VLPGESFGA RNQIDGEVV 
      FQTGMVGYP ESLTDPSYH AQILVLTYP LVGNYGVPK 
      AVEDPHGLP YFFESHRIW AAGLVVGEH CDNPSHWHN 
      FRTLSDWMK SENVPGIQG IDTRALTKK IREKGTILG 
      RIVYSLPIV TENLKIVDP NLRNLVEEV SVKKIVTYN 
      SGGSPRICA VDCGLKYNQ IRCLVNRGA RVDLVPWDH 
      KLNINDYDG LFLSNGPGD PTMCQVTIN NIKDVIKGP 
      TIKPIFGIC LGHQLLSVA IGCKSYKMK YGNRGHNQP 
      CTHHGTNRC YMTSQNHGF AINTETLPN DWEILFTNA 
      NDKTNEGII HSKLPFFSV QFHPEHTAG PQDLECLFD 
      VFLDLVKNP NQTAKTLLT QKLTYSGTP QLPERPKKV 
      LIIGSGGLS IGQAGEFDY SGSQAIKAL REENIQTVL 
      INPNIATVQ TSKGLADKV YFLPLVPDY VEQVIRVER 
      PGGVLLTFG GQTGLNCGV ELQKAGIFD KYNIQILGT 
      PIQAIIDTE DRKIFTERI AAIGEKVAP SMAAHSVEE 
      ALEAAEQLG YPVMARAAF SLGGLGSGF ANSKDELKT 
      LAVQALAHS SQLIIDKSL KGWKEVEYE VVRDAFDNC 
      ITVCNMENV DPLGIHTGE SIVVAPSQT LSNREYNML 
      RTTAIKVIR HFGVIGECN IQYAVNPNS EEFYIIEVN 
      ARLSRSSAL ASKATGYPL AYIAAKLAL GVPLPEINN 
      SVTGVTTAC FEPSLDYCV VKIPRWDLH KFSRVSTKI 
      GSSMKSVGE VMAIGRKFE EAFQKALRM VDENVMGFD 
      PYLKDVNDE ELKEPTDKR MFVVAAALK KGYSVDRLY 
      DLTKIDRWF LEKMKKIVD WTTFLETID QLHLSHDIL 
      LKAKQIGFS DKQIATAVK STELAIRKQ REEFGIKPY 
      VKQIDTVAA EWPATTNYL YLTYNAFEH DLTFEEKHT 
      MVIGSGVYR IGSSVEFDW CAVGCLREL RRLGRKTIM 
      INYNPETVS TDYDMSDRL YFEEISFEV VMDIYNIED 
      PVGIILSMG GQLPNNIAI DLHRQQARI LGTSPDSID 
      GAENRFKFS RMLDRIGIM QPRWKELTN LKSAIEFCE 
      EVGYPCLVR PSYVLSGAA MNVAHSNQD LETYLNAAS 
      DVSKEHPVV ISKFILESK EIDVDAVAC DGVILCMAV 
      SEHVENAGV HSGDATLVT PPQDINDET LGKITQICK 
      AIAASLEVT GPFNMQLIA KDNDLKVIE CNVRVSRSF 
      PFVSKTLDH DFVAMATRV MVGELVQPV DVLSGCGKV 
      GVKVPQFSF SRLAGADFM LGVEMASTG EVACFGDNR 
      YEAYLKAMM STGFSVPEK SILLSIGSF KHKMELLPS 
      MRVLQKMGY KLYASLGTA DFYNEHGVK VESVQWTFE 
      NIDAITSQG ELKHLAEFL SKKQFDLVI NLPMRNGGA 
      RRVSSFMTH GYRTRRLAI DYSIPLVTD VKCTKLLVE 
      ALRRIGTAP PMKTHTDCM SSRRLVKLP GLIDTHVHV 
      REPGATHKE DYASCTAAA LAGGVTMIC AMPNTNPAV 
      TNQETLTLV ENLARKNAR CDYAIYMGA SSDNYSAIP 
      ELAPQVAGL KMYLNETFT TLRLNDLNT WTKHLANWP 
      KKAPICVHA EGQTTAAVI LMASLQNRP IHICHVARK 
      EEILIIKAA KEKGLNITC EVCPHHLFL STEDVKTLG 
      VCKSQVRPV LMSPEDQQA LWDNLHVID CFATDHAPH 
      TLEEKTSSS PPPGFPGLE TILPLLLNA VNENKLTID 
      DIVTKFYRN PKKIFNLPD QPNTYVEID LDEEWTIPE 
      ATTYSKAKW TPFAGRKVK GAVHRVVLR GEVAFVDGQ 
      ILVQPGFGQ NVRTWDKKT FDEVIAPPT VTTGEYNDG 
      QTNEIFKKL LSGGGKVHF SATNELHVD SRLGVTSPT 
      PVGPRIRTG SFSQMEQMH HSLANKHIL SVDMFTKDQ 
      LNDIFNLAQ TFRVNVSKE RAVDHILKG KVMASIFYE 
      VSTRTSCSF AAAMQRLGG GVIYMDETS SSVKKGETL 
      EDSVAVMAG YADVVVLRH PTPGSVMRA SHHCRKPLI 
      NAGDGVGEH PSQALLDIF TIREEIGTV NGLTITLVG 
      DLKNGRTVH SLARLLTLY NVQLRYVSP PSLKMPSHI 
      TSFVASKGI PQNEHSTLE EVLPDTDVL YMTRIQRER 
      FSSQEEYDK ACGHFIVTP QLMTRAKRK MVVLHPLPR 
      VFEISPEFD TDPRAAYFR QAECGMYVR MALLAMVLG 
      KC
    CDS sequence
    • >TC012392 RA
      atgactaaa agtgacgaa agacacact tgttttctt 
      ctcctcgag gatgggact gtactaccg ggtgaatcg 
      tttggggct cgaaaccaa atcgatggg gaagttgtt 
      ttccagact gggatggtc ggttacccc gaatcccta 
      accgacccc agctaccat gcccagatc ctggtcctc 
      acatacccc ctcgtgggt aattacggt gtccccaaa 
      gcggtcgaa gacccgcac gggttgccc tattttttc 
      gaatcgcac cggatttgg gccgctggt ctcgtcgtg 
      ggtgaacac tgcgacaat ccgagccat tggcacaac 
      ttcaggaca ctttccgac tggatgaag tcggaaaat 
      gtccccggg atccaaggg attgacact agggccttg 
      accaagaaa attcgcgaa aaggggacg attttgggc 
      cggattgtt tatagtttg cccattgtg accgaaaat 
      ttgaaaatt gttgacccc aatttgagg aatttggtc 
      gaggaagtg tcagttaag aaaattgtg acgtataat 
      tccgggggg agtcccagg atttgtgcc gttgattgc 
      ggcttgaaa tacaatcaa attcgctgt ttggttaat 
      agaggggct cgggttgat ttggtacca tgggaccac 
      aaactcaat atcaacgac tatgatggt ctttttctc 
      agcaatggt cctggggac ccaaccatg tgccaggtc 
      actatcaat aacatcaaa gacgtgatt aagggacca 
      acaattaaa ccgattttt ggtatttgt ctcggtcac 
      caactcctt tcagtggcc attgggtgc aaaagctac 
      aaaatgaag tatggtaat cgtggtcac aaccaacca 
      tgcacccat catggtacc aaccggtgt tacatgacc 
      tcgcaaaac cacggtttt gccatcaat acggagaca 
      cttcccaac gactgggaa atcctcttc acgaatgct 
      aacgacaag accaacgaa ggaataatt cactcaaaa 
      ctgccgttt ttcagcgta cagttccac cccgaacac 
      acagctggg ccccaggac cttgaatgc ctcttcgac 
      gtgttcctg gacctggtt aagaacccc aaccagaca 
      gctaagact ctccttacc caaaaacta acctattcg 
      ggcacccct cagttgccc gaacgcccc aaaaaagtg 
      ctaataata ggttccggg ggcctttcc atcggccaa 
      gcgggcgag tttgactat tccggctcc caggccatt 
      aaagccctt cgtgaggaa aacatccaa actgttttg 
      atcaacccc aacatcgcc acagtccaa acctccaaa 
      ggtttagcc gacaaagtc tacttcttg cctctagta 
      cccgactac gtggagcaa gtcattcga gtcgagcgc 
      cccggaggc gtgctgtta accttcggg gggcaaacg 
      ggcctcaac tgtggggtc gagctccaa aaagccggc 
      atcttcgac aagtacaac atccaaatt ctggggacc 
      cccatccag gctataatc gatacggaa gaccggaag 
      atcttcacc gaacgtatt gccgccatt ggggagaag 
      gtggccccc agcatggcg gcgcactcc gtggaggag 
      gctctggag gcggccgag caactgggg tacccggtc 
      atggcccgc gctgccttt tccctaggc ggacttggg 
      tcgggcttc gccaatagt aaggacgaa cttaagact 
      ttggccgtc caggctttg gcccactcg agtcaattg 
      atcatcgac aagtcgctc aaaggctgg aaggaggtg 
      gagtacgag gtggtgcgg gacgccttc gacaactgc 
      atcacggtg tgcaacatg gagaacgtc gaccctttg 
      gggatacac accggggag tcgattgtg gtcgccccc 
      agccagacc ctttcgaac cgggagtac aacatgctg 
      cgcaccacc gccattaag gtgattcgg cacttcggg 
      gtcatcggc gagtgcaac atccagtac gccgtcaat 
      cccaactcc gaggagttc tacatcatc gaggttaat 
      gctaggtta tcgcgaagc tcggccctg gccagtaaa 
      gccacaggg tacccccta gcctacatc gcggccaag 
      ctggccttg ggggtaccc ctccctgag atcaataat 
      tccgtgact ggggtcaca actgcgtgt tttgagcca 
      agtcttgac tactgcgtc gtcaaaatc ccccgctgg 
      gacttgcac aagttctcc cgcgtcagt accaaaatc 
      ggaagttcg atgaaaagt gtgggggag gtgatggca 
      atcgggcgc aagttcgaa gaagcgttc cagaaggcc 
      ttgcgcatg gttgacgag aacgtgatg gggtttgac 
      ccctatctc aaggacgtt aacgacgag gagttgaag 
      gaacccacc gacaagagg atgtttgtg gtggcggcg 
      gccctcaag aaaggctat tcggtcgat aggctgtac 
      gacttgacc aaaatcgac cgctggttt ttggagaag 
      atgaagaag atcgtcgac tggaccacg ttcctcgaa 
      accatagac cagctgcac ttgtcgcat gatatttta 
      ctgaaggcc aaacaaatc gggttctcc gataaacag 
      attgcgaca gcagtcaag agtaccgaa ctggccatc 
      aggaagcaa cgcgaggag ttcgggatc aagccatac 
      gtcaagcag atagacaca gtggcggcg gaatggccc 
      gccaccacc aactacctc tacctaacc tacaacgcc 
      tttgagcac gacttgacc ttcgaggaa aaacacacc 
      atggtcata ggatcgggg gtttaccgc atcgggagc 
      tccgtggag ttcgactgg tgcgcagtc gggtgcctc 
      cgggagttg cgccgcttg ggccgcaag acaatcatg 
      atcaactac aaccccgaa accgtcagc accgactac 
      gacatgtcg gaccgcctc tactttgaa gaaatctcc 
      tttgaagtg gtcatggac atctacaac atcgaggac 
      cctgtcggg atcatactg tcaatgggc ggccaactc 
      cccaacaac atcgccatc gacctccac cggcagcaa 
      gcccgcatc ttgggcacg tcccccgac tccatcgac 
      ggcgccgag aaccgcttc aaattctcg cggatgttg 
      gaccggatc gggatcatg cagccgcgt tggaaggaa 
      ctcactaac ctcaaatcg gcgatcgag ttctgcgaa 
      gaagtgggg tacccgtgc ctggtgcgc ccctcctac 
      gtgctcagc ggcgccgcc atgaacgtt gcccactcc 
      aaccaggac ttggagacg tacctgaac gccgctagc 
      gacgtctcc aaggagcac ccagtcgtc atctcgaag 
      ttcatcctc gagtcgaag gagatcgac gtcgatgcg 
      gtggcctgc gacggcgtt atcctctgc atggcggtg 
      tcggagcac gtggagaac gccggggtg cactccggg 
      gacgccaca ctcgttacc ccgccgcag gacatcaac 
      gacgagact ctgggcaag atcacgcag atctgcaag 
      gccattgcg gcctcgctg gaggtgact gggcccttc 
      aacatgcaa ctgatcgcc aaggacaat gatctgaaa 
      gtgatcgag tgtaacgtg cgcgtttcg cgttccttc 
      ccctttgtt tccaaaacc ctagaccat gattttgtg 
      gcgatggcc actcgggtg atggttggg gagctggtc 
      cagcccgtg gatgtgctc tcggggtgc ggcaaagtg 
      ggggttaag gtgccccag ttttcgttt tcgcggctc 
      gcgggcgcc gattttatg ctgggggtg gagatggcg 
      tccactggg gaggtggcc tgttttggg gataatcgg 
      tacgaggcg tacttgaag gccatgatg agtactggg 
      tttagcgtc cccgaaaaa tcgattctg ctgtcaatt 
      gggagtttc aagcataaa atggagctg ctgcccagc 
      atgagagtg ttgcaaaag atggggtac aagttgtat 
      gcaagtttg ggcaccgct gacttttac aacgagcat 
      ggagttaag gtcgagtcg gtacaatgg actttcgag 
      aacattgat gccataacg agccaaggg gagctcaag 
      cacttggcg gagtttttg tcaaagaaa cagtttgac 
      ttggttatc aacctcccc atgcgcaat ggcggcgct 
      cggagagtg tcctccttc atgacccac gggtacagg 
      acccggcgc ctggccatc gactactcc atcccgctc 
      gtgaccgac gtcaaatgc accaaactc ctggtggag 
      gctctgaga cgcattggg accgcgccc cccatgaaa 
      acccacact gactgcatg tcttcgcgc cgtttggtc 
      aaactaccg ggccttatt gacacccac gtccacgtg 
      cgtgaaccc ggggccacc cacaaggag gactacgcc 
      tcctgcacg gccgcagcc ctcgctggc ggcgtcacc 
      atgatttgc gcgatgccc aacactaac ccggccgtc 
      accaaccag gaaacccta acccttgtg gagaacctg 
      gcgcggaaa aacgcccgg tgtgactat gccatctac 
      atgggggcc tcctcagac aactactcc gcgatcccg 
      gagctcgcc ccccaggtc gcaggcctc aaaatgtac 
      ctcaatgag accttcacc acgttgaga cttaacgat 
      ctcaacacc tggaccaag cacttggct aattggccg 
      aaaaaagcc ccgatttgt gtgcatgcc gaggggcaa 
      accacggcc gctgttatc ctgatggcg agtttgcaa 
      aaccggcca atccacatc tgtcacgtg gcccggaaa 
      gaggaaatc ttgatcatc aaagcggcc aaggaaaaa 
      ggcttgaac ataacctgt gaggtttgc ccccaccac 
      ctttttctc tcgactgaa gatgtcaaa accttgggg 
      gtgtgtaaa agtcaggtt cggcctgtt ctaatgtca 
      ccagaggac caacaagcc ctttgggat aacttacac 
      gtgattgac tgctttgcc accgaccat gccccccac 
      actttggag gagaagacc agttcgagc cctccccca 
      ggcttcccc gggctggag acaatcctc ccgttgctc 
      ctgaacgcc gttaacgag aacaaactg actattgac 
      gacatcgtc accaagttc taccgcaac cccaagaag 
      attttcaat ttgccggac caaccaaac acttacgtt 
      gaaatcgac ctggacgag gagtggacg attccagag 
      gctaccact tacagtaag gctaagtgg accccgttc 
      gctggccgc aaggtcaag ggagccgtc caccgcgtg 
      gtgctccgt ggagaggtg gctttcgtc gacggccag 
      atcctggtg cagcccggg tttgggcaa aacgtgcgc 
      acgtgggac aaaaagacc ttcgacgag gtcatagcc 
      cctcccacc gtcaccact ggggagtac aacgacggg 
      cagaccaac gagattttc aagaagttg ctgtcaggg 
      ggtggcaaa gtgcacttc tctgccacc aacgagctg 
      cacgtggac agccggctg ggggtgacc tcgcccacg 
      cccgtgggg ccccggatc cgcactggt agtttctcc 
      caaatggag cagatgcat cacagtctt gccaacaag 
      catattctt tcggtggat atgtttacg aaagaccag 
      ctcaatgat attttcaac ttggcgcaa acttttcgc 
      gttaatgtc agtaaggag cgggctgtt gatcatatc 
      ttgaagggg aaagtcatg gcgtcgatt ttctacgag 
      gttagcact cggaccagt tgcagtttc gcggctgcc 
      atgcagagg ttgggcggt ggggtgatt tatatggac 
      gagacgagt tcgtcggtg aagaaaggg gagactttg 
      gaggactcg gttgctgtg atggcgggg tatgccgat 
      gtggtggtg ttgaggcat ccgacgcct ggctcggtt 
      atgcgggcg tcccaccac tgccgcaag cccctcatc 
      aacgccggc gacggagtc ggcgagcac cccagccaa 
      gccctcctc gatattttc acaattcga gaggagatc 
      ggcactgtg aacggcctc accattacc ctagttggt 
      gatttgaaa aacgggcgc actgttcat tcccttgca 
      cgtttgctg acactgtac aacgtgcag ctgcgttac 
      gtaagcccc ccgagcttg aaaatgccg agtcatatc 
      accagcttc gtggcctcc aaaggcata ccccagaac 
      gagcacagt acccttgag gaggtactg ccggacacg 
      gacgtactg tacatgact cgcatccag agagaacgg 
      tttagctca caagaggag tacgacaag gcctgtggc 
      cactttatc gtaactccg caactgatg acgagggcg 
      aagaggaaa atggtggtg cttcacccc ctgcccagg 
      gtgttcgaa atcagcccg gagtttgac acggacccc 
      cgggcggcc tatttcagg caggcggag tgcgggatg 
      tatgtcaga atggcgctc ctagccatg gttttaggg 
      aagtgttaa
    mRNA sequence
    • >TC012392 RA
      atgactaaa agtgacgaa agacacact tgttttctt 
      ctcctcgag gatgggact gtactaccg ggtgaatcg 
      tttggggct cgaaaccaa atcgatggg gaagttgtt 
      ttccagact gggatggtc ggttacccc gaatcccta 
      accgacccc agctaccat gcccagatc ctggtcctc 
      acatacccc ctcgtgggt aattacggt gtccccaaa 
      gcggtcgaa gacccgcac gggttgccc tattttttc 
      gaatcgcac cggatttgg gccgctggt ctcgtcgtg 
      ggtgaacac tgcgacaat ccgagccat tggcacaac 
      ttcaggaca ctttccgac tggatgaag tcggaaaat 
      gtccccggg atccaaggg attgacact agggccttg 
      accaagaaa attcgcgaa aaggggacg attttgggc 
      cggattgtt tatagtttg cccattgtg accgaaaat 
      ttgaaaatt gttgacccc aatttgagg aatttggtc 
      gaggaagtg tcagttaag aaaattgtg acgtataat 
      tccgggggg agtcccagg atttgtgcc gttgattgc 
      ggcttgaaa tacaatcaa attcgctgt ttggttaat 
      agaggggct cgggttgat ttggtacca tgggaccac 
      aaactcaat atcaacgac tatgatggt ctttttctc 
      agcaatggt cctggggac ccaaccatg tgccaggtc 
      actatcaat aacatcaaa gacgtgatt aagggacca 
      acaattaaa ccgattttt ggtatttgt ctcggtcac 
      caactcctt tcagtggcc attgggtgc aaaagctac 
      aaaatgaag tatggtaat cgtggtcac aaccaacca 
      tgcacccat catggtacc aaccggtgt tacatgacc 
      tcgcaaaac cacggtttt gccatcaat acggagaca 
      cttcccaac gactgggaa atcctcttc acgaatgct 
      aacgacaag accaacgaa ggaataatt cactcaaaa 
      ctgccgttt ttcagcgta cagttccac cccgaacac 
      acagctggg ccccaggac cttgaatgc ctcttcgac 
      gtgttcctg gacctggtt aagaacccc aaccagaca 
      gctaagact ctccttacc caaaaacta acctattcg 
      ggcacccct cagttgccc gaacgcccc aaaaaagtg 
      ctaataata ggttccggg ggcctttcc atcggccaa 
      gcgggcgag tttgactat tccggctcc caggccatt 
      aaagccctt cgtgaggaa aacatccaa actgttttg 
      atcaacccc aacatcgcc acagtccaa acctccaaa 
      ggtttagcc gacaaagtc tacttcttg cctctagta 
      cccgactac gtggagcaa gtcattcga gtcgagcgc 
      cccggaggc gtgctgtta accttcggg gggcaaacg 
      ggcctcaac tgtggggtc gagctccaa aaagccggc 
      atcttcgac aagtacaac atccaaatt ctggggacc 
      cccatccag gctataatc gatacggaa gaccggaag 
      atcttcacc gaacgtatt gccgccatt ggggagaag 
      gtggccccc agcatggcg gcgcactcc gtggaggag 
      gctctggag gcggccgag caactgggg tacccggtc 
      atggcccgc gctgccttt tccctaggc ggacttggg 
      tcgggcttc gccaatagt aaggacgaa cttaagact 
      ttggccgtc caggctttg gcccactcg agtcaattg 
      atcatcgac aagtcgctc aaaggctgg aaggaggtg 
      gagtacgag gtggtgcgg gacgccttc gacaactgc 
      atcacggtg tgcaacatg gagaacgtc gaccctttg 
      gggatacac accggggag tcgattgtg gtcgccccc 
      agccagacc ctttcgaac cgggagtac aacatgctg 
      cgcaccacc gccattaag gtgattcgg cacttcggg 
      gtcatcggc gagtgcaac atccagtac gccgtcaat 
      cccaactcc gaggagttc tacatcatc gaggttaat 
      gctaggtta tcgcgaagc tcggccctg gccagtaaa 
      gccacaggg tacccccta gcctacatc gcggccaag 
      ctggccttg ggggtaccc ctccctgag atcaataat 
      tccgtgact ggggtcaca actgcgtgt tttgagcca 
      agtcttgac tactgcgtc gtcaaaatc ccccgctgg 
      gacttgcac aagttctcc cgcgtcagt accaaaatc 
      ggaagttcg atgaaaagt gtgggggag gtgatggca 
      atcgggcgc aagttcgaa gaagcgttc cagaaggcc 
      ttgcgcatg gttgacgag aacgtgatg gggtttgac 
      ccctatctc aaggacgtt aacgacgag gagttgaag 
      gaacccacc gacaagagg atgtttgtg gtggcggcg 
      gccctcaag aaaggctat tcggtcgat aggctgtac 
      gacttgacc aaaatcgac cgctggttt ttggagaag 
      atgaagaag atcgtcgac tggaccacg ttcctcgaa 
      accatagac cagctgcac ttgtcgcat gatatttta 
      ctgaaggcc aaacaaatc gggttctcc gataaacag 
      attgcgaca gcagtcaag agtaccgaa ctggccatc 
      aggaagcaa cgcgaggag ttcgggatc aagccatac 
      gtcaagcag atagacaca gtggcggcg gaatggccc 
      gccaccacc aactacctc tacctaacc tacaacgcc 
      tttgagcac gacttgacc ttcgaggaa aaacacacc 
      atggtcata ggatcgggg gtttaccgc atcgggagc 
      tccgtggag ttcgactgg tgcgcagtc gggtgcctc 
      cgggagttg cgccgcttg ggccgcaag acaatcatg 
      atcaactac aaccccgaa accgtcagc accgactac 
      gacatgtcg gaccgcctc tactttgaa gaaatctcc 
      tttgaagtg gtcatggac atctacaac atcgaggac 
      cctgtcggg atcatactg tcaatgggc ggccaactc 
      cccaacaac atcgccatc gacctccac cggcagcaa 
      gcccgcatc ttgggcacg tcccccgac tccatcgac 
      ggcgccgag aaccgcttc aaattctcg cggatgttg 
      gaccggatc gggatcatg cagccgcgt tggaaggaa 
      ctcactaac ctcaaatcg gcgatcgag ttctgcgaa 
      gaagtgggg tacccgtgc ctggtgcgc ccctcctac 
      gtgctcagc ggcgccgcc atgaacgtt gcccactcc 
      aaccaggac ttggagacg tacctgaac gccgctagc 
      gacgtctcc aaggagcac ccagtcgtc atctcgaag 
      ttcatcctc gagtcgaag gagatcgac gtcgatgcg 
      gtggcctgc gacggcgtt atcctctgc atggcggtg 
      tcggagcac gtggagaac gccggggtg cactccggg 
      gacgccaca ctcgttacc ccgccgcag gacatcaac 
      gacgagact ctgggcaag atcacgcag atctgcaag 
      gccattgcg gcctcgctg gaggtgact gggcccttc 
      aacatgcaa ctgatcgcc aaggacaat gatctgaaa 
      gtgatcgag tgtaacgtg cgcgtttcg cgttccttc 
      ccctttgtt tccaaaacc ctagaccat gattttgtg 
      gcgatggcc actcgggtg atggttggg gagctggtc 
      cagcccgtg gatgtgctc tcggggtgc ggcaaagtg 
      ggggttaag gtgccccag ttttcgttt tcgcggctc 
      gcgggcgcc gattttatg ctgggggtg gagatggcg 
      tccactggg gaggtggcc tgttttggg gataatcgg 
      tacgaggcg tacttgaag gccatgatg agtactggg 
      tttagcgtc cccgaaaaa tcgattctg ctgtcaatt 
      gggagtttc aagcataaa atggagctg ctgcccagc 
      atgagagtg ttgcaaaag atggggtac aagttgtat 
      gcaagtttg ggcaccgct gacttttac aacgagcat 
      ggagttaag gtcgagtcg gtacaatgg actttcgag 
      aacattgat gccataacg agccaaggg gagctcaag 
      cacttggcg gagtttttg tcaaagaaa cagtttgac 
      ttggttatc aacctcccc atgcgcaat ggcggcgct 
      cggagagtg tcctccttc atgacccac gggtacagg 
      acccggcgc ctggccatc gactactcc atcccgctc 
      gtgaccgac gtcaaatgc accaaactc ctggtggag 
      gctctgaga cgcattggg accgcgccc cccatgaaa 
      acccacact gactgcatg tcttcgcgc cgtttggtc 
      aaactaccg ggccttatt gacacccac gtccacgtg 
      cgtgaaccc ggggccacc cacaaggag gactacgcc 
      tcctgcacg gccgcagcc ctcgctggc ggcgtcacc 
      atgatttgc gcgatgccc aacactaac ccggccgtc 
      accaaccag gaaacccta acccttgtg gagaacctg 
      gcgcggaaa aacgcccgg tgtgactat gccatctac 
      atgggggcc tcctcagac aactactcc gcgatcccg 
      gagctcgcc ccccaggtc gcaggcctc aaaatgtac 
      ctcaatgag accttcacc acgttgaga cttaacgat 
      ctcaacacc tggaccaag cacttggct aattggccg 
      aaaaaagcc ccgatttgt gtgcatgcc gaggggcaa 
      accacggcc gctgttatc ctgatggcg agtttgcaa 
      aaccggcca atccacatc tgtcacgtg gcccggaaa 
      gaggaaatc ttgatcatc aaagcggcc aaggaaaaa 
      ggcttgaac ataacctgt gaggtttgc ccccaccac 
      ctttttctc tcgactgaa gatgtcaaa accttgggg 
      gtgtgtaaa agtcaggtt cggcctgtt ctaatgtca 
      ccagaggac caacaagcc ctttgggat aacttacac 
      gtgattgac tgctttgcc accgaccat gccccccac 
      actttggag gagaagacc agttcgagc cctccccca 
      ggcttcccc gggctggag acaatcctc ccgttgctc 
      ctgaacgcc gttaacgag aacaaactg actattgac 
      gacatcgtc accaagttc taccgcaac cccaagaag 
      attttcaat ttgccggac caaccaaac acttacgtt 
      gaaatcgac ctggacgag gagtggacg attccagag 
      gctaccact tacagtaag gctaagtgg accccgttc 
      gctggccgc aaggtcaag ggagccgtc caccgcgtg 
      gtgctccgt ggagaggtg gctttcgtc gacggccag 
      atcctggtg cagcccggg tttgggcaa aacgtgcgc 
      acgtgggac aaaaagacc ttcgacgag gtcatagcc 
      cctcccacc gtcaccact ggggagtac aacgacggg 
      cagaccaac gagattttc aagaagttg ctgtcaggg 
      ggtggcaaa gtgcacttc tctgccacc aacgagctg 
      cacgtggac agccggctg ggggtgacc tcgcccacg 
      cccgtgggg ccccggatc cgcactggt agtttctcc 
      caaatggag cagatgcat cacagtctt gccaacaag 
      catattctt tcggtggat atgtttacg aaagaccag 
      ctcaatgat attttcaac ttggcgcaa acttttcgc 
      gttaatgtc agtaaggag cgggctgtt gatcatatc 
      ttgaagggg aaagtcatg gcgtcgatt ttctacgag 
      gttagcact cggaccagt tgcagtttc gcggctgcc 
      atgcagagg ttgggcggt ggggtgatt tatatggac 
      gagacgagt tcgtcggtg aagaaaggg gagactttg 
      gaggactcg gttgctgtg atggcgggg tatgccgat 
      gtggtggtg ttgaggcat ccgacgcct ggctcggtt 
      atgcgggcg tcccaccac tgccgcaag cccctcatc 
      aacgccggc gacggagtc ggcgagcac cccagccaa 
      gccctcctc gatattttc acaattcga gaggagatc 
      ggcactgtg aacggcctc accattacc ctagttggt 
      gatttgaaa aacgggcgc actgttcat tcccttgca 
      cgtttgctg acactgtac aacgtgcag ctgcgttac 
      gtaagcccc ccgagcttg aaaatgccg agtcatatc 
      accagcttc gtggcctcc aaaggcata ccccagaac 
      gagcacagt acccttgag gaggtactg ccggacacg 
      gacgtactg tacatgact cgcatccag agagaacgg 
      tttagctca caagaggag tacgacaag gcctgtggc 
      cactttatc gtaactccg caactgatg acgagggcg 
      aagaggaaa atggtggtg cttcacccc ctgcccagg 
      gtgttcgaa atcagcccg gagtttgac acggacccc 
      cgggcggcc tatttcagg caggcggag tgcgggatg 
      tatgtcaga atggcgctc ctagccatg gttttaggg 
      aagtgttaa
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