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Gene information
General information
TC012388 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6034225..6044307
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012388
    iBeetle screen iB_05232

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012388 RA
      MSYLQSNPN RPISQSHYQ GGPGDLPMP PAKEQTLMW 
      QQNSYMGDS GIHSGAATQ VPSLSGKEE DMDLFDLDQ 
      GYSQGFTQD QVDEMNNQL NQTRSQRVR AAMFPETLD 
      EGIEIPSTQ FDPSHQTAV QRLSEPSQM LKHAVVNLI 
      NYQDDADLA TRAIPELIK LLNDEDQVV VSQAAMMVH 
      QLSKKEASR HAIMNSPQM VAALVRAIS NSNDLETTK 
      GAVGTLHKL SHHRQGLLA IFKSGGIPA LVKLLSSPV 
      ESVLFYAIT TLHNLLLHQ DGSKMAVRL AGGLQKMVA 
      LLQRNNVKF LAIVTDCLQ ILAYGNQES KLIILASQG 
      PIELVRIMR SYDYEKLLW TTSRVLKVL SVCPSNKPA 
      IVEAGGMQA LAMHLGNPS GRLVQNCLW TLRNLSDAA 
      TKVDGLEGL LSSLVQVLN SQDVHVVTC ASGILSNLT 
      CNNQRNKVT VCQVGGVDA LVRTIVSAG DREEITEPA 
      VCALRHLTS RHVESEMAQ NAVRLNYGI QVIVKLLNP 
      PSRWPLVKA VIGLIRNLA LCPANHAPL REHGAIHHL 
      VQLLMRAFQ DTQRRSSVA SGGSQQASS YADGVRMEE 
      IVEGTVGAL HILARESHN RAIIRQQMV IPIFVQLLF 
      NDIENIQRV AAGVLCELA ADKEGAEMI EQEGATSPL 
      TELLHSRNE GVATYAAAV LFRMSEDKP QDYKKRLSM 
      ELTNSLFRE ENLWNPDLG IDPDLQDIL SPDQAYDTM 
      YAQGPPSVH SNHGGRPYQ QQGYDQIPI DMQGLEIGS 
      HAGGNGSTY SQIEAMDVA NGDPDINFD PINQLPAPP 
      QDNNQVAAW YDTDL
    CDS sequence
    • >TC012388 RA
      atgagttac ttacagtcc aacccgaat agacccatc 
      tcgcagagc cactatcag gggggcccc ggtgattta 
      cccatgccg cccgcgaaa gaacagacc ctcatgtgg 
      caacaaaac tcgtacatg ggcgactcc ggcatccac 
      tccggagcc gccacccag gtaccatcc ctgagcggg 
      aaggaagaa gacatggac ctctttgac ttggatcaa 
      ggatactcc caaggcttc acccaggat caggtcgac 
      gaaatgaac aaccagctc aaccagacg cgctcgcag 
      agggtcaga gctgccatg tttccggag actttggac 
      gaagggatc gaaatcccc tcgactcag ttcgatccc 
      agccaccag acggccgtt caaaggctc tccgagccg 
      agtcaaatg ctcaaacat gcagtcgtc aatctaatc 
      aattatcaa gacgacgcg gatttggcg actcgtgcc 
      atccccgag ctgatcaaa ctattgaac gacgaagac 
      caagtggtt gtttcgcaa gccgcgatg atggtccac 
      cagctctcc aagaaagag gcttcgagg cacgcgatc 
      atgaacagt ccgcaaatg gtggcagcg ctagtgagg 
      gccatttcc aactcgaat gacctggaa acgaccaaa 
      ggtgcagtc ggcaccttg cacaaatta tcgcaccat 
      cggcaaggt ctcctggcc atcttcaaa agcggggga 
      attccagca ttggtcaaa ctgctgagc tccccggtt 
      gaaagtgtg ctcttttac gccatcacc accttgcac 
      aatttattg cttcaccaa gacggctcc aaaatggcc 
      gtccgctta gccggaggc ttgcaaaaa atggtcgcg 
      ttgttgcaa cgaaacaac gtcaagttc ttggcaatt 
      gtgaccgac tgtttgcaa atcctggcg tacggcaac 
      caggagagc aaactgatt attctggca tcgcaaggt 
      cccatcgaa ctcgtgaga atcatgcgg tcgtacgac 
      tacgagaag ttgctctgg acgacgtcc cgagtcttg 
      aaagtccta tccgtgtgt ccgagcaac aagccggcc 
      atcgtcgaa gccggcggc atgcaagcc ctggccatg 
      cacctgggg aaccccagc ggccgcctc gtgcagaac 
      tgcctctgg accttgcgt aacctctcc gatgccgcg 
      accaaagtc gacggtctc gaaggcttg ctcagttcc 
      ctggtgcag gttttgaac tcgcaagac gtccacgtc 
      gttacttgc gcttctggt attttgtct aacctgacg 
      tgtaacaac caacgcaac aaagtcacc gtttgccaa 
      gtcggtggt gttgatgcc cttgtgcgc acaattgtg 
      agcgctggg gatcgtgag gagatcaca gaaccggcc 
      gtttgcgct ttgcgacat ctcacgtcg agacatgtg 
      gagtcggaa atggcgcaa aatgcagtc aggttgaat 
      tacggtatt caagttatc gtgaagttg ttgaatccg 
      ccatcgcgt tggccgcta gtcaaggcc gttatcggg 
      cttatcagg aatttagcg ctgtgtccg gccaatcat 
      gcaccacta agggagcat ggggcgata catcatttg 
      gtgcagctg ctgatgagg gccttccag gacacacag 
      aggaggtcg agtgtggcg agcggcggc agccagcaa 
      gcctcgagt tacgccgat ggcgtacga atggaggag 
      atcgtcgaa gggacagtg ggggcgttg cacatattg 
      gctcgggaa tcccacaac agggccatt atacgccaa 
      caaatggtt ataccaata tttgttcag ttgcttttt 
      aacgatatt gaaaatatc caacgtgtg gcagctggg 
      gtgttgtgc gaattggcg gctgataaa gaaggggcg 
      gaaatgatc gagcaagag ggcgccaca tcgccacta 
      accgaattg cttcattcc cgaaacgaa ggggtggct 
      acgtacgct gccgcagta ttatttagg atgagcgag 
      gacaagcca caagactac aaaaaacgg ctcagtatg 
      gaactgaca aattcgctg ttccgggag gagaatttg 
      tggaatccg gatctgggc atcgatcct gatttacag 
      gacattttg agtcctgac caagcatat gatacaatg 
      tatgcccaa ggaccccct agtgtgcac agcaatcac 
      ggtggacgg ccgtaccaa cagcaaggg tatgaccag 
      ataccaata gatatgcaa gggctagag attgggtcg 
      cacgctggc gggaacggg tcgacgtac agccagatt 
      gaagccatg gatgtagcc aatggcgac cccgacatc 
      aactttgac ccaattaat caattacct gcacctccc 
      caagacaac aatcaagtt gcagcatgg tacgatacc 
      gatttataa
    mRNA sequence
    • >TC012388 RA
      ccaatctga tatttgtga ttgggtgcg gtgttgttg 
      tgttcgtta tcgtatatt atcgtaatt taaagggaa 
      aaagtgatt ttgtggcgc aaggtaagt tatttcggt 
      gtaaaatta tcaatttca ttcatttgt aagtgtgag 
      agtgagttt tttgtgtgg tttggccaa gttttaaaa 
      atgtcgatg aactgtgta tgacaacat ggatctctt 
      tgtgagtgg gtgattctg attctgatt tagtccgat 
      gtctgatcg gtttgtgat tgcaatctt tggaaattg 
      tgatcgtta cacaaacga agcgtctgg tgtttaatt 
      aaatgtttc ggtttgaaa gcacgtaac gattcctca 
      tccggcaat tccttgtca ctgctcgaa attccaatg 
      agattggtc atgagattc agagaattc catgtttca 
      ggtgtggtg tggtaattc gccatgagt tacttacag 
      tccaacccg aatagaccc atctcgcag agccactat 
      caggggggc cccggtgat ttacccatg ccgcccgcg 
      aaagaacag accctcatg tggcaacaa aactcgtac 
      atgggcgac tccggcatc cactccgga gccgccacc 
      caggtacca tccctgagc gggaaggaa gaagacatg 
      gacctcttt gacttggat caaggatac tcccaaggc 
      ttcacccag gatcaggtc gacgaaatg aacaaccag 
      ctcaaccag acgcgctcg cagagggtc agagctgcc 
      atgtttccg gagactttg gacgaaggg atcgaaatc 
      ccctcgact cagttcgat cccagccac cagacggcc 
      gttcaaagg ctctccgag ccgagtcaa atgctcaaa 
      catgcagtc gtcaatcta atcaattat caagacgac 
      gcggatttg gcgactcgt gccatcccc gagctgatc 
      aaactattg aacgacgaa gaccaagtg gttgtttcg 
      caagccgcg atgatggtc caccagctc tccaagaaa 
      gaggcttcg aggcacgcg atcatgaac agtccgcaa 
      atggtggca gcgctagtg agggccatt tccaactcg 
      aatgacctg gaaacgacc aaaggtgca gtcggcacc 
      ttgcacaaa ttatcgcac catcggcaa ggtctcctg 
      gccatcttc aaaagcggg ggaattcca gcattggtc 
      aaactgctg agctccccg gttgaaagt gtgctcttt 
      tacgccatc accaccttg cacaattta ttgcttcac 
      caagacggc tccaaaatg gccgtccgc ttagccgga 
      ggcttgcaa aaaatggtc gcgttgttg caacgaaac 
      aacgtcaag ttcttggca attgtgacc gactgtttg 
      caaatcctg gcgtacggc aaccaggag agcaaactg 
      attattctg gcatcgcaa ggtcccatc gaactcgtg 
      agaatcatg cggtcgtac gactacgag aagttgctc 
      tggacgacg tcccgagtc ttgaaagtc ctatccgtg 
      tgtccgagc aacaagccg gccatcgtc gaagccggc 
      ggcatgcaa gccctggcc atgcacctg gggaacccc 
      agcggccgc ctcgtgcag aactgcctc tggaccttg 
      cgtaacctc tccgatgcc gcgaccaaa gtcgacggt 
      ctcgaaggc ttgctcagt tccctggtg caggttttg 
      aactcgcaa gacgtccac gtcgttact tgcgcttct 
      ggtattttg tctaacctg acgtgtaac aaccaacgc 
      aacaaagtc accgtttgc caagtcggt ggtgttgat 
      gcccttgtg cgcacaatt gtgagcgct ggggatcgt 
      gaggagatc acagaaccg gccgtttgc gctttgcga 
      catctcacg tcgagacat gtggagtcg gaaatggcg 
      caaaatgca gtcaggttg aattacggt attcaagtt 
      atcgtgaag ttgttgaat ccgccatcg cgttggccg 
      ctagtcaag gccgttatc gggcttatc aggaattta 
      gcgctgtgt ccggccaat catgcacca ctaagggag 
      catggggcg atacatcat ttggtgcag ctgctgatg 
      agggccttc caggacaca cagaggagg tcgagtgtg 
      gcgagcggc ggcagccag caagcctcg agttacgcc 
      gatggcgta cgaatggag gagatcgtc gaagggaca 
      gtgggggcg ttgcacata ttggctcgg gaatcccac 
      aacagggcc attatacgc caacaaatg gttatacca 
      atatttgtt cagttgctt tttaacgat attgaaaat 
      atccaacgt gtggcagct ggggtgttg tgcgaattg 
      gcggctgat aaagaaggg gcggaaatg atcgagcaa 
      gagggcgcc acatcgcca ctaaccgaa ttgcttcat 
      tcccgaaac gaaggggtg gctacgtac gctgccgca 
      gtattattt aggatgagc gaggacaag ccacaagac 
      tacaaaaaa cggctcagt atggaactg acaaattcg 
      ctgttccgg gaggagaat ttgtggaat ccggatctg 
      ggcatcgat cctgattta caggacatt ttgagtcct 
      gaccaagca tatgataca atgtatgcc caaggaccc 
      cctagtgtg cacagcaat cacggtgga cggccgtac 
      caacagcaa gggtatgac cagatacca atagatatg 
      caagggcta gagattggg tcgcacgct ggcgggaac 
      gggtcgacg tacagccag attgaagcc atggatgta 
      gccaatggc gaccccgac atcaacttt gacccaatt 
      aatcaatta cctgcacct ccccaagac aacaatcaa 
      gttgcagca tggtacgat accgattta taatacctt 
      cctcgatgt cttttgttt gatgaaatt agacatttt 
      cattttatt tttcttgat aatcccaaa tgcgacact 
      aggaagttt cgttgttaa aatactata gtgtaccta 
      tttataatt tattagcaa gtatgtata aaatatcat 
      atacgtgta taagtttcg gttgtataa tattgagtc 
      tgatttgtt taaaataaa aaaatacat tttatgctg 
      tctgattat tatcaaaga gctaaaaca caatgctga 
      tttttgact gatgtgtta tggttgtcg tttacaaaa 
      atatctaaa tgtgattgt acaaaataa atcgtcatg 
      taccgtttt cttttttat taataaaag tgttggtat 
      attatttgt tttttattt tgttgtgag tgctgctgc 
      tactattgc cgttttatg taacgtcaa aggtgtagt 
      gttaacatt gtgttttaa ttcttatca tatttaaac 
      aatttcatg aacagggta gaacttttt aaggcactg 
      cctttgaaa tgtatgcat gtctttgta ggaaggaat 
      aaactttct cattgtat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY