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Gene information
General information
TC012384 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5942936..5950279
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012384
    iBeetle screen iB_05231

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012384 RA
      MADSRLVNF ITCCRLCLS DSVDDLKSV FDESVDDRG 
      LQQKISACL GVEITSDDK LSTMVCLQC IEKINNWQS 
      YKLTCCENQ NKLEQWINT SNPELSLSD MNIQIKEEP 
      IDNDILTPE VVMNGEIPV KNEPMEEEY NELPPPLTP 
      QADDMIDVE LVTNSPSSL TTGTDEPGN AYDPKKCSF 
      CGKLFSSSG NRKKHERKM HGPRKTHSV TQSDNGSLS 
      QDEERSQSS VDSQPPSEP PPKEPTSEE KKILFAAGL 
      KLLQRNSTP PIAFENLSK IENSYIEKC KAMVTMHNT 
      LKCACHNVT HPNLKGLLS HLRSLRIWF PVFTCYHCM 
      ITFTDRSTG TKHYLRCPK SNLETLIKL SNLKKRSEV 
      KTRLYQNYK CTICRFMYT FHEDFCTHV DEDHSHISL 
      PYTCSCGRV FTSLEDYKD HAYISCLVE FYCDVCFVT 
      VKTLDAFQK HAAEVHDNS EGFVLLQED NYKRRKSSP 
      GATQTRQSV KREHTDERN IISGKRRTS YKPPIIDPE 
      DDEDTKVLN PLFKHKLGG TVCPICNKE YSTNNNMWR 
      HYKTHVQDK KEEEFDDSI YCCPDCGGM FSTAEWEKH 
      KGMHKKKTC KECGKIFQF QSELDQHRS VHLNLKLYR 
      DSKTQNYKS TMASPTNEL NNIVMMCEI CDRMFTSKE 
      QLKEHKLTH ETMPVLESS VPTNGEENK TKKYRCKIC 
      DKQYAGYGG LWDHNRKKH PDHKSPQSH EFPKKCEYC 
      DKIFYTGGS YFMHRQMHE RNNQKKLEA PQLQPATND 
      EEPNEEDPE ESYHTCKRC FKVFSNRCN LKNHMKSHG 
      ISTSPSTKS PKKGAKKVW CDICHCAFE NTASLERHK 
      SEDHADDGM PALSDDQSE VDLKTPFVF TCDVCVQTF 
      TTKIALKKH KEKHAREVR PVMKNKPLV YCKYCKISF 
      ANPFQLTNH MNEEHMEDC KPPPKKEKT KQFVCKICG 
      KNFTTASAL YAHQGWHKR GKFESEDKP ESPVPRIKE 
      EPKTETHFE CRTCNAVLP NDTALQIHI LEKHRNVNA 
      TVLPARCTM CNLDFNSQD EYDQHKRFH AMVEKQPKA 
      KTWACTQCP AGFSRADML KMHVKQFHK EVKEYRCHH 
      CDRVFEKGN SLSIHLKVH EKQKLVSGG KASPVPVSK 
      STKALYSCS ICNKGFDLP KDLRMHTIQ AHPF
    CDS sequence
    • >TC012384 RA
      atggcggac tcgcggctg gttaatttc attacgtgc 
      tgcagactg tgcttgtcg gacagtgtg gacgatcta 
      aagtcggtt tttgatgaa agtgtagac gatcggggg 
      ttacagcag aagatttcg gcctgtttg ggagtggag 
      ataacatcc gacgataaa ctctccacg atggtctgc 
      ctccagtgt atcgaaaaa atcaacaat tggcagtcc 
      tacaaactc acatgttgc gagaatcaa aacaaacta 
      gaacaatgg ataaacacg tccaatccc gagctatcc 
      ctctcagac atgaacatc caaatcaag gaagaaccc 
      atcgataac gacatcctc acaccggaa gtcgtcatg 
      aacggcgag atccccgtc aaaaacgag cccatggaa 
      gaagaatac aacgaactc ccgccaccc ctaaccccc 
      caagccgat gacatgatc gacgtcgaa ctcgtcaca 
      aactcccca tcttcccta accaccggc accgatgag 
      ccaggtaac gcatacgat ccaaaaaaa tgtagcttt 
      tgcggcaag ttgttcagc tcgtcgggc aaccgcaag 
      aaacacgaa cggaaaatg catggccct agaaaaacc 
      cactcggtt acccaatcc gacaacggt tcgttgagt 
      caggacgag gagaggtcg caaagctcg gtcgattcc 
      cagcccccg agcgaaccg ccaccgaag gaacccaca 
      agcgaggag aaaaaaatc ctgtttgct gcaggtctt 
      aaactgctg cagcgcaac tccaccccg cccatagcc 
      ttcgaaaat ttgtcgaaa attgaaaat agctacatc 
      gagaaatgc aaggctatg gtgaccatg cacaacacc 
      ctcaagtgt gcgtgccac aacgtcacg catccgaac 
      ctgaagggg ctgctgtcc cacttgcgt tcgctgcgg 
      atttggttc ccggttttc acttgttac cactgtatg 
      atcacgttc acggacaga tcgacgggc acaaagcac 
      tatctccgg tgtccaaag tcgaattta gaaactctg 
      attaaatta tccaatttg aagaagcgg agcgaagtc 
      aagacgcga ctttaccag aactacaaa tgcacgatt 
      tgtcgcttc atgtacact ttccacgag gatttctgc 
      actcatgtt gacgaagac cacagccat atcagccta 
      ccgtacact tgctcctgc gggcgcgtg ttcacctcc 
      ctggaggac tacaaggac catgcttac atcagctgt 
      ctggtcgag ttctactgt gatgtttgt tttgttact 
      gttaagact ttggacgcg ttccaaaaa cacgccgct 
      gaggtgcat gacaattcg gagggtttt gttctactc 
      caggaggat aattacaag aggaggaag agctctccc 
      ggagcgacg cagacgagg cagagtgtt aagcgggag 
      catacggac gagcgtaat attatttcg gggaagagg 
      aggacgtcc tacaagccc ccgattatc gaccctgag 
      gacgatgag gacactaag gtacttaac ccgttgttc 
      aagcacaag ttgggaggg acggtttgt ccgatctgc 
      aacaaggaa tactcgact aataacaac atgtggcgg 
      cactacaag acccacgtt caggataag aaggaagag 
      gagtttgat gactcgatt tattgttgt cccgactgt 
      ggcgggatg ttcagtacc gccgaatgg gagaagcac 
      aaggggatg cacaagaag aaaacgtgc aaagagtgc 
      gggaagatc ttccagttc cagtcggag ctggaccag 
      cacaggagc gtccacctc aatttgaaa ctgtacaga 
      gactcgaaa acgcaaaac tacaagagc acgatggct 
      agccccacc aacgagctc aataacatt gttatgatg 
      tgcgagatt tgtgataga atgttcacc tcgaaggag 
      cagttgaaa gaacacaaa ctgactcac gagaccatg 
      cccgtgctg gaatcgagc gtgcccaca aacggcgaa 
      gagaacaaa acgaagaag taccgttgc aagatctgt 
      gataagcaa tatgcaggt tatggcggt ctgtgggac 
      cacaacagg aagaagcat ccggaccac aagtcgccc 
      caaagtcac gagttccca aagaaatgc gaatactgc 
      gataagatt ttctacacg ggtggttcg tacttcatg 
      catcgacag atgcatgag cggaacaac cagaagaaa 
      ttggaagcg ccgcagctc caaccggcc accaatgac 
      gaggagccg aacgaagaa gaccccgaa gagtcctac 
      catacgtgc aaacgctgc tttaaggtg ttctcaaac 
      cggtgcaac ctcaagaac catatgaag agtcacggc 
      attagcacc agccccagc acgaagagc cccaaaaag 
      ggggcgaag aaagtctgg tgtgacatt tgccactgt 
      gctttcgaa aacacagca agcttggag agacacaag 
      tctgaggac cacgccgat gatggaatg ccggcgctt 
      tcggacgac caaagcgaa gtcgacttg aagaccccc 
      ttcgtcttc acgtgcgac gtttgcgtg caaaccttc 
      accacgaaa atcgccctg aagaagcac aaggagaag 
      cacgcacgt gaggtgcga cccgtgatg aagaacaaa 
      ccgctggtg tattgcaag tactgcaaa atatccttc 
      gccaatccc ttccagctg accaaccac atgaacgag 
      gagcacatg gaggactgc aagccgccc ccgaagaag 
      gagaagacc aagcagttc gtgtgcaag atctgcggc 
      aagaacttc acgacggcc tccgccttg tacgcgcac 
      caagggtgg cacaagcgc ggcaaattc gagagtgag 
      gacaagccg gagtcgccg gtgcctcgg attaaggag 
      gaaccaaag acagagacg cacttcgag tgccggacg 
      tgcaacgca gtgctgccg aatgacacg gcgctgcag 
      atacatatc ctggagaag caccggaat gtgaacgcg 
      acggtgctg ccggccagg tgtacgatg tgcaacctg 
      gatttcaac tcgcaggat gagtacgac cagcataag 
      cggttccat gccatggtt gagaagcaa cccaaggcg 
      aaaacctgg gcttgtacg cagtgcccg gccgggttc 
      agtcgggcg gatatgttg aagatgcat gttaagcag 
      tttcataag gaggtgaag gagtaccgg tgccatcac 
      tgtgaccgg gtgttcgaa aaggggaat tcgttgagt 
      attcatttg aaggtgcat gagaagcag aagttggtg 
      tcagggggg aaggcgtct ccggtgccg gttagtaag 
      agcacgaag gctttgtac tcgtgttcg atttgtaat 
      aaagggttt gatttgccc aaagatctg aggatgcac 
      accatacag gcgcatccg ttctga
    mRNA sequence
    • >TC012384 RA
      aaggacgtc actgggttt tcaactgaa cttttgcaa 
      aatggggat gcccatgaa gtattttga gttacgatg 
      atatttgat accggcttt tgaagtatc aaagccctg 
      actaacttc gttatttgc atttcaatc cgggctaaa 
      agtgctaaa gtcggctca attttgccg ggaactgct 
      caaatgacg cgtcctgga ggcagttcc ggggcttgg 
      ggacggtat tcggttttt gcggaaggt gcatttaga 
      tcagttcag ttttacagg gggttgcat ttcgcaaga 
      agtgtttga aggagtttc gtggtgctg atgaatcga 
      taaaaacga gtttaatcg gtgcgtagt ctcgggtga 
      gttaaaggt gaaagcgga gtaccagag gagtgtttg 
      tgggtttga atctaggaa caagggcgg gtcaagccc 
      catgtgtgc tttccttgg tgctggaag aggaatttt 
      ccttgggtg aagagtact gagtgcggc gaaaaggca 
      cacgctgct gttcttccc accattggc agctgagcg 
      accagcgtt ccgttgaat atcccgtca tgagtgggg 
      agttgatag tcatggcgt acccgttcc aggcagtca 
      gcgttcgta gaaacatgt ccccgtttt agcatgtgt 
      gccgaatcc ggggtgaaa agttggtga aaatcgcgg 
      ggaaagggt cgaggtcct gtttgagag tgcggtttt 
      gaggggatc acgatggcg gactcgcgg ctggttaat 
      ttcattacg tgctgcaga ctgtgcttg tcggacagt 
      gtggacgat ctaaagtcg gtttttgat gaaagtgta 
      gacgatcgg gggttacag cagaagatt tcggcctgt 
      ttgggagtg gagataaca tccgacgat aaactctcc 
      acgatggtc tgcctccag tgtatcgaa aaaatcaac 
      aattggcag tcctacaaa ctcacatgt tgcgagaat 
      caaaacaaa ctagaacaa tggataaac acgtccaat 
      cccgagcta tccctctca gacatgaac atccaaatc 
      aaggaagaa cccatcgat aacgacatc ctcacaccg 
      gaagtcgtc atgaacggc gagatcccc gtcaaaaac 
      gagcccatg gaagaagaa tacaacgaa ctcccgcca 
      cccctaacc ccccaagcc gatgacatg atcgacgtc 
      gaactcgtc acaaactcc ccatcttcc ctaaccacc 
      ggcaccgat gagccaggt aacgcatac gatccaaaa 
      aaatgtagc ttttgcggc aagttgttc agctcgtcg 
      ggcaaccgc aagaaacac gaacggaaa atgcatggc 
      cctagaaaa acccactcg gttacccaa tccgacaac 
      ggttcgttg agtcaggac gaggagagg tcgcaaagc 
      tcggtcgat tcccagccc ccgagcgaa ccgccaccg 
      aaggaaccc acaagcgag gagaaaaaa atcctgttt 
      gctgcaggt cttaaactg ctgcagcgc aactccacc 
      ccgcccata gccttcgaa aatttgtcg aaaattgaa 
      aatagctac atcgagaaa tgcaaggct atggtgacc 
      atgcacaac accctcaag tgtgcgtgc cacaacgtc 
      acgcatccg aacctgaag gggctgctg tcccacttg 
      cgttcgctg cggatttgg ttcccggtt ttcacttgt 
      taccactgt atgatcacg ttcacggac agatcgacg 
      ggcacaaag cactatctc cggtgtcca aagtcgaat 
      ttagaaact ctgattaaa ttatccaat ttgaagaag 
      cggagcgaa gtcaagacg cgactttac cagaactac 
      aaatgcacg atttgtcgc ttcatgtac actttccac 
      gaggatttc tgcactcat gttgacgaa gaccacagc 
      catatcagc ctaccgtac acttgctcc tgcgggcgc 
      gtgttcacc tccctggag gactacaag gaccatgct 
      tacatcagc tgtctggtc gagttctac tgtgatgtt 
      tgttttgtt actgttaag actttggac gcgttccaa 
      aaacacgcc gctgaggtg catgacaat tcggagggt 
      tttgttcta ctccaggag gataattac aagaggagg 
      aagagctct cccggagcg acgcagacg aggcagagt 
      gttaagcgg gagcatacg gacgagcgt aatattatt 
      tcggggaag aggaggacg tcctacaag cccccgatt 
      atcgaccct gaggacgat gaggacact aaggtactt 
      aacccgttg ttcaagcac aagttggga gggacggtt 
      tgtccgatc tgcaacaag gaatactcg actaataac 
      aacatgtgg cggcactac aagacccac gttcaggat 
      aagaaggaa gaggagttt gatgactcg atttattgt 
      tgtcccgac tgtggcggg atgttcagt accgccgaa 
      tgggagaag cacaagggg atgcacaag aagaaaacg 
      tgcaaagag tgcgggaag atcttccag ttccagtcg 
      gagctggac cagcacagg agcgtccac ctcaatttg 
      aaactgtac agagactcg aaaacgcaa aactacaag 
      agcacgatg gctagcccc accaacgag ctcaataac 
      attgttatg atgtgcgag atttgtgat agaatgttc 
      acctcgaag gagcagttg aaagaacac aaactgact 
      cacgagacc atgcccgtg ctggaatcg agcgtgccc 
      acaaacggc gaagagaac aaaacgaag aagtaccgt 
      tgcaagatc tgtgataag caatatgca ggttatggc 
      ggtctgtgg gaccacaac aggaagaag catccggac 
      cacaagtcg ccccaaagt cacgagttc ccaaagaaa 
      tgcgaatac tgcgataag attttctac acgggtggt 
      tcgtacttc atgcatcga cagatgcat gagcggaac 
      aaccagaag aaattggaa gcgccgcag ctccaaccg 
      gccaccaat gacgaggag ccgaacgaa gaagacccc 
      gaagagtcc taccatacg tgcaaacgc tgctttaag 
      gtgttctca aaccggtgc aacctcaag aaccatatg 
      aagagtcac ggcattagc accagcccc agcacgaag 
      agccccaaa aagggggcg aagaaagtc tggtgtgac 
      atttgccac tgtgctttc gaaaacaca gcaagcttg 
      gagagacac aagtctgag gaccacgcc gatgatgga 
      atgccggcg ctttcggac gaccaaagc gaagtcgac 
      ttgaagacc cccttcgtc ttcacgtgc gacgtttgc 
      gtgcaaacc ttcaccacg aaaatcgcc ctgaagaag 
      cacaaggag aagcacgca cgtgaggtg cgacccgtg 
      atgaagaac aaaccgctg gtgtattgc aagtactgc 
      aaaatatcc ttcgccaat cccttccag ctgaccaac 
      cacatgaac gaggagcac atggaggac tgcaagccg 
      cccccgaag aaggagaag accaagcag ttcgtgtgc 
      aagatctgc ggcaagaac ttcacgacg gcctccgcc 
      ttgtacgcg caccaaggg tggcacaag cgcggcaaa 
      ttcgagagt gaggacaag ccggagtcg ccggtgcct 
      cggattaag gaggaacca aagacagag acgcacttc 
      gagtgccgg acgtgcaac gcagtgctg ccgaatgac 
      acggcgctg cagatacat atcctggag aagcaccgg 
      aatgtgaac gcgacggtg ctgccggcc aggtgtacg 
      atgtgcaac ctggatttc aactcgcag gatgagtac 
      gaccagcat aagcggttc catgccatg gttgagaag 
      caacccaag gcgaaaacc tgggcttgt acgcagtgc 
      ccggccggg ttcagtcgg gcggatatg ttgaagatg 
      catgttaag cagtttcat aaggaggtg aaggagtac 
      cggtgccat cactgtgac cgggtgttc gaaaagggg 
      aattcgttg agtattcat ttgaaggtg catgagaag 
      cagaagttg gtgtcaggg gggaaggcg tctccggtg 
      ccggttagt aagagcacg aaggctttg tactcgtgt 
      tcgatttgt aataaaggg tttgatttg cccaaagat 
      ctgaggatg cacaccata caggcgcat ccgttctga 
      cgtatattg tataagtag gattttata taagtttat 
      gatcagttg ttgatttaa gataattat tttttgcac 
      tttatttaa cttcaaact gttattggt aacgattgt 
      ttctcattg tctgtgcct aagaactgt aaatatatt 
      tagattaaa acatgatgt aggatgtag gtatttttt 
      acaataaat atgtctgtt taca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY