| General information | |||
| TC012383 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 5911466..5936102 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012383 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05230 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC012383 RA
MEAESPPLH STIATHTET ITEEDPLNR RLNSSDEAD SSITGFNSL ANVTVIEQP VVVCEGSDS GVEVVETND LYQRALSSN SGNSHDFDA LRSCDSSII SCCSNYEDA YNLLVRRNS TLIEDYTRN GDVTSENGS ESSSVTGSS SSRNSKRTN LANKKKVNL QDNKTKERS RSKPPVPRR GENPIRVKS IDRLQGKTT KKLLPLDLS KKDTKRPSS GRKTPSVTP TDDGRWPSI NSKPAPLMA RSLRGTMET PKTKQETKT IEKYATLPR RRKEKSNEE VRKISRDEI TRLATPKTK PGSREVTPS KNFLTKKKQ KVKIYHETA IQTALTMTD VTKALAGLN VSPMSPKDA EKCDKSEQA DLSVKEIEL LGAQLKDLK DKYEALGRE HKSQSEKLL QTEGKLREE IVEKEGLRE ELKTNQERI LAILGENNP SDDDANVSS DSLLVLESR FQNVSQVVI RQEEEISKL NSYCRSLQI DLEKSLASQ KILVQQQQE LEAESIELQ EFMQAEKTT LSDALKDAE TEIAKHQKM LEKKDKELN EKIEECKRV TKLNEQRWQ ENLSLQARM GALEAKSRD LLVHQGSSV SGTAVALSA LIGRLEGLV DELVTSYSI SDQELEDVI FHNEAYSNS SSSPDATPE KSRKFLGDK TPSPKKGSS FVSAVINAI KNAAAQSPF AGHKDISKD NLCTENSSS ELLDSETEP SLMMEHVLE DVVVPDGHT HNMISSCHS ILSTQSDSF VDMSRSNYS TEMPLCEIF PSFSLVDQV IDVDNLVTR LLKVIRIIQ LENEDCTNE LQEQRDRLS EQVDKQKET NKLVSKQLK DWEILGARL KTEVKELMH QLSKKNNEM DSIKCELNR QREEVEKLN QDVCELSTA LAKAELEMK MRDEEISEA LKNWEENNE IPPPEVIAR LNAAQTEVP LLKEKLSQK ERHLSELTQ EFLASRQVL TESLKEAVN EAKKQYEAI DNALEVLHN NQNVVQQCT PLAELQREL ENTNFQSAS AMPLAAPAD CNANAALLQ AIANLQINT TA
- >TC012383 RA
atggaagct gaatcgcct cctctccat tcaaccatt gcaactcac accgaaacc atcaccgaa gaggatcca ctcaacaga agactgaac agcagcgac gaagccgac agttccatc accggcttc aacagtctg gccaacgtc accgtcatc gagcagccg gtggtggtg tgtgaaggc agtgacagt ggtgtcgaa gttgtcgaa acaaacgat ttataccaa cgtgcttta agttcaaac agcggcaac tcgcacgat tttgacgcc ctgagatcg tgcgactcc tcaatcatc agctgctgc tccaactac gaggacgcc tacaacctc ctagtgcgg aggaactcc accctgatc gaggactac accaggaat ggggacgtg acaagcgag aacggcagc gagagttct tcagtgacc ggaagctcg tcctccaga aactcaaaa cggacgaac cttgccaac aaaaagaaa gtcaacctg caggacaac aagacgaag gagcgctcc aggtccaag cctccggtg cccagacga ggcgaaaac ccgatccgg gtcaaatca atcgacaga ctccaaggg aaaaccacc aagaagctg ctgccgctc gacttgtcc aagaaagac acgaaacgg ccgtcctca ggccgcaaa accccaagt gtcacgccc acagatgac ggcagatgg ccctcgatt aacagcaaa ccggccccc ttaatggcc aggtctctc cggggcacc atggagacc ccaaaaacc aagcaagag accaaaact atcgaaaaa tacgcaact cttccgcga cgaagaaaa gaaaaatca aacgaagaa gtcagaaaa atcagtcgt gacgaaatc acgcgactg gcaaccccg aagaccaag ccagggtct cgtgaagtg acaccgtcc aaaaacttc ctaacaaag aaaaagcaa aaagtcaaa atttaccat gaaacggcc atacaaact gccctgacg atgaccgac gttacgaag gctctcgcc ggcctaaac gtgagccct atgagcccc aaggacgcg gaaaagtgc gataagagc gaacaggcc gacttgagc gtcaaagaa atcgagctg cttggggca caactcaaa gatttgaaa gacaaatat gaagcgctc gggagagag cataaatca caaagtgag aaactctta caaactgag ggtaagtta cgagaggag atcgtggag aaagagggc ttgagagag gagctgaag acgaaccag gagcgcatt ttggcgatt ctcggcgag aacaacccg agtgatgat gatgcaaat gtctctagt gatagcctt ctggtgcta gaatcccgt ttccaaaac gtcagccag gttgtgatc cgccaagag gaagaaatc tccaaactc aactcctac tgtcgctcg cttcaaatc gacctcgag aaaagccta gcctcccag aaaatcctc gtccaacaa caacaagaa ctggaagcc gaaagcata gaactccaa gagttcatg caagccgaa aaaaccacc ctttccgat gcactaaaa gacgcagaa accgaaatt gcaaaacac caaaaaatg ctggaaaag aaggacaag gaactgaac gagaaaatc gaggagtgt aaacgtgtg acgaaactg aacgaacag agatggcag gagaatttg agtttacag gcacgcatg ggcgccctc gaggccaaa agtagggac ttactggtg caccaaggc agttcggtg tcggggact gcggtggct ttgtcagcg ctgatcggg cgcttggag gggcttgtc gatgaactc gttacgtct tacagtatt tcggaccag gagttggag gacgttatc ttccataac gaagcttac agtaacagt agtagcagt ccggacgct accccggag aagagccgg aaattcctc ggggataaa accccgtct ccgaaaaaa ggttcctcg ttcgtttcc gctgtcata aacgccatc aaaaacgcc gcggctcag agtcctttt gcgggccac aaggacata agcaaggac aatctgtgc acggagaat tccagctcc gagctgctg gactctgaa actgagcct tcgctgatg atggagcat gtgctggag gacgtggtg gtccctgac ggtcacacc cataacatg atttcgtca tgtcatagc attttaagc acgcaaagt gatagtttt gttgatatg tctaggagt aattattcg accgaaatg cctttatgt gaaatattc ccgtcgttt tccctggtt gaccaggtt atagacgtc gataatttg gtcacgcgg ctgttgaaa gtcataaga ataatccaa ctggaaaac gaagactgc actaacgaa ctgcaggag cagagagac aggctttcc gaacaagtc gacaagcaa aaagagacg aacaaactc gtaagcaag caactgaaa gactgggaa atactcgga gcgcggcta aagactgaa gttaaagaa ctgatgcac cagttgagc aagaaaaac aacgaaatg gacagcatt aagtgcgaa cttaatagg caaagagag gaagtcgag aaactgaac caagatgtg tgcgaactg tcaacggcc ctagccaag gccgagctt gagatgaag atgcgagac gaagaaata tccgaagcg ctcaaaaac tgggaagaa aacaacgaa ataccacct cctgaagta atcgcgcga ctaaacgcg gctcagact gaagtccct cttttgaaa gagaaacta tcgcaaaaa gaacgacat ttgagtgag ctcactcaa gaattcctc gccagtaga caagtcctg actgaaagc ttgaaagaa gcggtaaac gaggcgaaa aagcagtac gaagcgatt gacaatgct ttagaggtt ctccacaac aatcaaaac gtggtgcaa cagtgcaca ccgttggcc gagctgcag agggaattg gaaaatacg aacttccaa agtgcgtcc gcaatgccg ttggcggca ccagccgat tgcaacgca aatgcggct cttttgcaa gcgattgct aatttacag atcaacacc actgcctaa
- >TC012383 RA
tttgtgata caggggtcg tacgacttt cccacccaa aagctctag ctgctgctg gagttggta gcggctgtt ggctccact actcaccaa caacaaacg agtggagtg gaacagttc gttgtagtc gttagtgct cgtggtttg tgttgtcgc gttgtgtta ttatttttg ttttaatta tttttgggc tccaaatac accccaggc aagggcagt gaaggaatg aataactaa accgcctca ccatggaag ctgaatcgc ctcctctcc attcaacca ttgcaactc acaccgaaa ccatcaccg aagaggatc cactcaaca gaagactga acagcagcg acgaagccg acagttcca tcaccggct tcaacagtc tggccaacg tcaccgtca tcgagcagc cggtggtgg tgtgtgaag gcagtgaca gtggtgtcg aagttgtcg aaacaaacg atttatacc aacgtgctt taagttcaa acagcggca actcgcacg attttgacg ccctgagat cgtgcgact cctcaatca tcagctgct gctccaact acgaggacg cctacaacc tcctagtgc ggaggaact ccaccctga tcgaggact acaccagga atggggacg tgacaagcg agaacggca gcgagagtt cttcagtga ccggaagct cgtcctcca gaaactcaa aacggacga accttgcca acaaaaaga aagtcaacc tgcaggaca acaagacga aggagcgct ccaggtcca agcctccgg tgcccagac gaggcgaaa acccgatcc gggtcaaat caatcgaca gactccaag ggaaaacca ccaagaagc tgctgccgc tcgacttgt ccaagaaag acacgaaac ggccgtcct caggccgca aaaccccaa gtgtcacgc ccacagatg acggcagat ggccctcga ttaacagca aaccggccc ccttaatgg ccaggtctc tccggggca ccatggaga ccccaaaaa ccaagcaag agaccaaaa ctatcgaaa aatacgcaa ctcttccgc gacgaagaa aagaaaaat caaacgaag aagtcagaa aaatcagtc gtgacgaaa tcacgcgac tggcaaccc cgaagacca agccagggt ctcgtgaag tgacaccgt ccaaaaact tcctaacaa agaaaaagc aaaaagtca aaatttacc atgaaacgg ccatacaaa ctgccctga cgatgaccg acgttacga aggctctcg ccggcctaa acgtgagcc ctatgagcc ccaaggacg cggaaaagt gcgataaga gcgaacagg ccgacttga gcgtcaaag aaatcgagc tgcttgggg cacaactca aagatttga aagacaaat atgaagcgc tcgggagag agcataaat cacaaagtg agaaactct tacaaactg agggtaagt tacgagagg agatcgtgg agaaagagg gcttgagag aggagctga agacgaacc aggagcgca ttttggcga ttctcggcg agaacaacc cgagtgatg atgatgcaa atgtctcta gtgatagcc ttctggtgc tagaatccc gtttccaaa acgtcagcc aggttgtga tccgccaag aggaagaaa tctccaaac tcaactcct actgtcgct cgcttcaaa tcgacctcg agaaaagcc tagcctccc agaaaatcc tcgtccaac aacaacaag aactggaag ccgaaagca tagaactcc aagagttca tgcaagccg aaaaaacca ccctttccg atgcactaa aagacgcag aaaccgaaa ttgcaaaac accaaaaaa tgctggaaa agaaggaca aggaactga acgagaaaa tcgaggagt gtaaacgtg tgacgaaac tgaacgaac agagatggc aggagaatt tgagtttac aggcacgca tgggcgccc tcgaggcca aaagtaggg acttactgg tgcaccaag gcagttcgg tgtcgggga ctgcggtgg ctttgtcag cgctgatcg ggcgcttgg aggggcttg tcgatgaac tcgttacgt cttacagta tttcggacc aggagttgg aggacgtta tcttccata acgaagctt acagtaaca gtagtagca gtccggacg ctaccccgg agaagagcc ggaaattcc tcggggata aaaccccgt ctccgaaaa aaggttcct cgttcgttt ccgctgtca taaacgcca tcaaaaacg ccgcggctc agagtcctt ttgcgggcc acaaggaca taagcaagg acaatctgt gcacggaga attccagct ccgagctgc tggactctg aaactgagc cttcgctga tgatggagc atgtgctgg aggacgtgg tggtccctg acggtcaca cccataaca tgatttcgt catgtcata gcattttaa gcacgcaaa gtgatagtt ttgttgata tgtctagga gtaattatt cgaccgaaa tgcctttat gtgaaatat tcccgtcgt tttccctgg ttgaccagg ttatagacg tcgataatt tggtcacgc ggctgttga aagtcataa gaataatcc aactggaaa acgaagact gcactaacg aactgcagg agcagagag acaggcttt ccgaacaag tcgacaagc aaaaagaga cgaacaaac tcgtaagca agcaactga aagactggg aaatactcg gagcgcggc taaagactg aagttaaag aactgatgc accagttga gcaagaaaa acaacgaaa tggacagca ttaagtgcg aacttaata ggcaaagag aggaagtcg agaaactga accaagatg tgtgcgaac tgtcaacgg ccctagcca aggccgagc ttgagatga agatgcgag acgaagaaa tatccgaag cgctcaaaa actgggaag aaaacaacg aaataccac ctcctgaag taatcgcgc gactaaacg cggctcaga ctgaagtcc ctcttttga aagagaaac tatcgcaaa aagaacgac atttgagtg agctcactc aagaattcc tcgccagta gacaagtcc tgactgaaa gcttgaaag aagcggtaa acgaggcga aaaagcagt acgaagcga ttgacaatg ctttagagg ttctccaca acaatcaaa acgtggtgc aacagtgca caccgttgg ccgagctgc agagggaat tggaaaata cgaacttcc aaagtgcgt ccgcaatgc cgttggcgg caccagccg attgcaacg caaatgcgg ctcttttgc aagcgattg ctaatttac agatcaaca ccactgcct aagattgtg aaagtcaca atgatagtg atgccattg attttgact atgatttgt acataaatc ggtttttat ttaagtttt tcctagaga tttaaaagg ctgaattat gccattcta cgtccaagt gtacgaatt ttaacagta ggaggagtc gttccagac tgttggacc cgttgcagt taaacagac actaaggct ttcttcact tttacaaaa tttttacgt catttgttt gaaatcggt gcttttgat aaatccata tttttagat tgtactatt atgccattg ttgtaaata aataaatta cgtagacct gt
