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Gene information
General information
TC012383 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5911466..5936102
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012383
    iBeetle screen iB_05230

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012383 RA
      MEAESPPLH STIATHTET ITEEDPLNR RLNSSDEAD 
      SSITGFNSL ANVTVIEQP VVVCEGSDS GVEVVETND 
      LYQRALSSN SGNSHDFDA LRSCDSSII SCCSNYEDA 
      YNLLVRRNS TLIEDYTRN GDVTSENGS ESSSVTGSS 
      SSRNSKRTN LANKKKVNL QDNKTKERS RSKPPVPRR 
      GENPIRVKS IDRLQGKTT KKLLPLDLS KKDTKRPSS 
      GRKTPSVTP TDDGRWPSI NSKPAPLMA RSLRGTMET 
      PKTKQETKT IEKYATLPR RRKEKSNEE VRKISRDEI 
      TRLATPKTK PGSREVTPS KNFLTKKKQ KVKIYHETA 
      IQTALTMTD VTKALAGLN VSPMSPKDA EKCDKSEQA 
      DLSVKEIEL LGAQLKDLK DKYEALGRE HKSQSEKLL 
      QTEGKLREE IVEKEGLRE ELKTNQERI LAILGENNP 
      SDDDANVSS DSLLVLESR FQNVSQVVI RQEEEISKL 
      NSYCRSLQI DLEKSLASQ KILVQQQQE LEAESIELQ 
      EFMQAEKTT LSDALKDAE TEIAKHQKM LEKKDKELN 
      EKIEECKRV TKLNEQRWQ ENLSLQARM GALEAKSRD 
      LLVHQGSSV SGTAVALSA LIGRLEGLV DELVTSYSI 
      SDQELEDVI FHNEAYSNS SSSPDATPE KSRKFLGDK 
      TPSPKKGSS FVSAVINAI KNAAAQSPF AGHKDISKD 
      NLCTENSSS ELLDSETEP SLMMEHVLE DVVVPDGHT 
      HNMISSCHS ILSTQSDSF VDMSRSNYS TEMPLCEIF 
      PSFSLVDQV IDVDNLVTR LLKVIRIIQ LENEDCTNE 
      LQEQRDRLS EQVDKQKET NKLVSKQLK DWEILGARL 
      KTEVKELMH QLSKKNNEM DSIKCELNR QREEVEKLN 
      QDVCELSTA LAKAELEMK MRDEEISEA LKNWEENNE 
      IPPPEVIAR LNAAQTEVP LLKEKLSQK ERHLSELTQ 
      EFLASRQVL TESLKEAVN EAKKQYEAI DNALEVLHN 
      NQNVVQQCT PLAELQREL ENTNFQSAS AMPLAAPAD 
      CNANAALLQ AIANLQINT TA
    CDS sequence
    • >TC012383 RA
      atggaagct gaatcgcct cctctccat tcaaccatt 
      gcaactcac accgaaacc atcaccgaa gaggatcca 
      ctcaacaga agactgaac agcagcgac gaagccgac 
      agttccatc accggcttc aacagtctg gccaacgtc 
      accgtcatc gagcagccg gtggtggtg tgtgaaggc 
      agtgacagt ggtgtcgaa gttgtcgaa acaaacgat 
      ttataccaa cgtgcttta agttcaaac agcggcaac 
      tcgcacgat tttgacgcc ctgagatcg tgcgactcc 
      tcaatcatc agctgctgc tccaactac gaggacgcc 
      tacaacctc ctagtgcgg aggaactcc accctgatc 
      gaggactac accaggaat ggggacgtg acaagcgag 
      aacggcagc gagagttct tcagtgacc ggaagctcg 
      tcctccaga aactcaaaa cggacgaac cttgccaac 
      aaaaagaaa gtcaacctg caggacaac aagacgaag 
      gagcgctcc aggtccaag cctccggtg cccagacga 
      ggcgaaaac ccgatccgg gtcaaatca atcgacaga 
      ctccaaggg aaaaccacc aagaagctg ctgccgctc 
      gacttgtcc aagaaagac acgaaacgg ccgtcctca 
      ggccgcaaa accccaagt gtcacgccc acagatgac 
      ggcagatgg ccctcgatt aacagcaaa ccggccccc 
      ttaatggcc aggtctctc cggggcacc atggagacc 
      ccaaaaacc aagcaagag accaaaact atcgaaaaa 
      tacgcaact cttccgcga cgaagaaaa gaaaaatca 
      aacgaagaa gtcagaaaa atcagtcgt gacgaaatc 
      acgcgactg gcaaccccg aagaccaag ccagggtct 
      cgtgaagtg acaccgtcc aaaaacttc ctaacaaag 
      aaaaagcaa aaagtcaaa atttaccat gaaacggcc 
      atacaaact gccctgacg atgaccgac gttacgaag 
      gctctcgcc ggcctaaac gtgagccct atgagcccc 
      aaggacgcg gaaaagtgc gataagagc gaacaggcc 
      gacttgagc gtcaaagaa atcgagctg cttggggca 
      caactcaaa gatttgaaa gacaaatat gaagcgctc 
      gggagagag cataaatca caaagtgag aaactctta 
      caaactgag ggtaagtta cgagaggag atcgtggag 
      aaagagggc ttgagagag gagctgaag acgaaccag 
      gagcgcatt ttggcgatt ctcggcgag aacaacccg 
      agtgatgat gatgcaaat gtctctagt gatagcctt 
      ctggtgcta gaatcccgt ttccaaaac gtcagccag 
      gttgtgatc cgccaagag gaagaaatc tccaaactc 
      aactcctac tgtcgctcg cttcaaatc gacctcgag 
      aaaagccta gcctcccag aaaatcctc gtccaacaa 
      caacaagaa ctggaagcc gaaagcata gaactccaa 
      gagttcatg caagccgaa aaaaccacc ctttccgat 
      gcactaaaa gacgcagaa accgaaatt gcaaaacac 
      caaaaaatg ctggaaaag aaggacaag gaactgaac 
      gagaaaatc gaggagtgt aaacgtgtg acgaaactg 
      aacgaacag agatggcag gagaatttg agtttacag 
      gcacgcatg ggcgccctc gaggccaaa agtagggac 
      ttactggtg caccaaggc agttcggtg tcggggact 
      gcggtggct ttgtcagcg ctgatcggg cgcttggag 
      gggcttgtc gatgaactc gttacgtct tacagtatt 
      tcggaccag gagttggag gacgttatc ttccataac 
      gaagcttac agtaacagt agtagcagt ccggacgct 
      accccggag aagagccgg aaattcctc ggggataaa 
      accccgtct ccgaaaaaa ggttcctcg ttcgtttcc 
      gctgtcata aacgccatc aaaaacgcc gcggctcag 
      agtcctttt gcgggccac aaggacata agcaaggac 
      aatctgtgc acggagaat tccagctcc gagctgctg 
      gactctgaa actgagcct tcgctgatg atggagcat 
      gtgctggag gacgtggtg gtccctgac ggtcacacc 
      cataacatg atttcgtca tgtcatagc attttaagc 
      acgcaaagt gatagtttt gttgatatg tctaggagt 
      aattattcg accgaaatg cctttatgt gaaatattc 
      ccgtcgttt tccctggtt gaccaggtt atagacgtc 
      gataatttg gtcacgcgg ctgttgaaa gtcataaga 
      ataatccaa ctggaaaac gaagactgc actaacgaa 
      ctgcaggag cagagagac aggctttcc gaacaagtc 
      gacaagcaa aaagagacg aacaaactc gtaagcaag 
      caactgaaa gactgggaa atactcgga gcgcggcta 
      aagactgaa gttaaagaa ctgatgcac cagttgagc 
      aagaaaaac aacgaaatg gacagcatt aagtgcgaa 
      cttaatagg caaagagag gaagtcgag aaactgaac 
      caagatgtg tgcgaactg tcaacggcc ctagccaag 
      gccgagctt gagatgaag atgcgagac gaagaaata 
      tccgaagcg ctcaaaaac tgggaagaa aacaacgaa 
      ataccacct cctgaagta atcgcgcga ctaaacgcg 
      gctcagact gaagtccct cttttgaaa gagaaacta 
      tcgcaaaaa gaacgacat ttgagtgag ctcactcaa 
      gaattcctc gccagtaga caagtcctg actgaaagc 
      ttgaaagaa gcggtaaac gaggcgaaa aagcagtac 
      gaagcgatt gacaatgct ttagaggtt ctccacaac 
      aatcaaaac gtggtgcaa cagtgcaca ccgttggcc 
      gagctgcag agggaattg gaaaatacg aacttccaa 
      agtgcgtcc gcaatgccg ttggcggca ccagccgat 
      tgcaacgca aatgcggct cttttgcaa gcgattgct 
      aatttacag atcaacacc actgcctaa
    mRNA sequence
    • >TC012383 RA
      tttgtgata caggggtcg tacgacttt cccacccaa 
      aagctctag ctgctgctg gagttggta gcggctgtt 
      ggctccact actcaccaa caacaaacg agtggagtg 
      gaacagttc gttgtagtc gttagtgct cgtggtttg 
      tgttgtcgc gttgtgtta ttatttttg ttttaatta 
      tttttgggc tccaaatac accccaggc aagggcagt 
      gaaggaatg aataactaa accgcctca ccatggaag 
      ctgaatcgc ctcctctcc attcaacca ttgcaactc 
      acaccgaaa ccatcaccg aagaggatc cactcaaca 
      gaagactga acagcagcg acgaagccg acagttcca 
      tcaccggct tcaacagtc tggccaacg tcaccgtca 
      tcgagcagc cggtggtgg tgtgtgaag gcagtgaca 
      gtggtgtcg aagttgtcg aaacaaacg atttatacc 
      aacgtgctt taagttcaa acagcggca actcgcacg 
      attttgacg ccctgagat cgtgcgact cctcaatca 
      tcagctgct gctccaact acgaggacg cctacaacc 
      tcctagtgc ggaggaact ccaccctga tcgaggact 
      acaccagga atggggacg tgacaagcg agaacggca 
      gcgagagtt cttcagtga ccggaagct cgtcctcca 
      gaaactcaa aacggacga accttgcca acaaaaaga 
      aagtcaacc tgcaggaca acaagacga aggagcgct 
      ccaggtcca agcctccgg tgcccagac gaggcgaaa 
      acccgatcc gggtcaaat caatcgaca gactccaag 
      ggaaaacca ccaagaagc tgctgccgc tcgacttgt 
      ccaagaaag acacgaaac ggccgtcct caggccgca 
      aaaccccaa gtgtcacgc ccacagatg acggcagat 
      ggccctcga ttaacagca aaccggccc ccttaatgg 
      ccaggtctc tccggggca ccatggaga ccccaaaaa 
      ccaagcaag agaccaaaa ctatcgaaa aatacgcaa 
      ctcttccgc gacgaagaa aagaaaaat caaacgaag 
      aagtcagaa aaatcagtc gtgacgaaa tcacgcgac 
      tggcaaccc cgaagacca agccagggt ctcgtgaag 
      tgacaccgt ccaaaaact tcctaacaa agaaaaagc 
      aaaaagtca aaatttacc atgaaacgg ccatacaaa 
      ctgccctga cgatgaccg acgttacga aggctctcg 
      ccggcctaa acgtgagcc ctatgagcc ccaaggacg 
      cggaaaagt gcgataaga gcgaacagg ccgacttga 
      gcgtcaaag aaatcgagc tgcttgggg cacaactca 
      aagatttga aagacaaat atgaagcgc tcgggagag 
      agcataaat cacaaagtg agaaactct tacaaactg 
      agggtaagt tacgagagg agatcgtgg agaaagagg 
      gcttgagag aggagctga agacgaacc aggagcgca 
      ttttggcga ttctcggcg agaacaacc cgagtgatg 
      atgatgcaa atgtctcta gtgatagcc ttctggtgc 
      tagaatccc gtttccaaa acgtcagcc aggttgtga 
      tccgccaag aggaagaaa tctccaaac tcaactcct 
      actgtcgct cgcttcaaa tcgacctcg agaaaagcc 
      tagcctccc agaaaatcc tcgtccaac aacaacaag 
      aactggaag ccgaaagca tagaactcc aagagttca 
      tgcaagccg aaaaaacca ccctttccg atgcactaa 
      aagacgcag aaaccgaaa ttgcaaaac accaaaaaa 
      tgctggaaa agaaggaca aggaactga acgagaaaa 
      tcgaggagt gtaaacgtg tgacgaaac tgaacgaac 
      agagatggc aggagaatt tgagtttac aggcacgca 
      tgggcgccc tcgaggcca aaagtaggg acttactgg 
      tgcaccaag gcagttcgg tgtcgggga ctgcggtgg 
      ctttgtcag cgctgatcg ggcgcttgg aggggcttg 
      tcgatgaac tcgttacgt cttacagta tttcggacc 
      aggagttgg aggacgtta tcttccata acgaagctt 
      acagtaaca gtagtagca gtccggacg ctaccccgg 
      agaagagcc ggaaattcc tcggggata aaaccccgt 
      ctccgaaaa aaggttcct cgttcgttt ccgctgtca 
      taaacgcca tcaaaaacg ccgcggctc agagtcctt 
      ttgcgggcc acaaggaca taagcaagg acaatctgt 
      gcacggaga attccagct ccgagctgc tggactctg 
      aaactgagc cttcgctga tgatggagc atgtgctgg 
      aggacgtgg tggtccctg acggtcaca cccataaca 
      tgatttcgt catgtcata gcattttaa gcacgcaaa 
      gtgatagtt ttgttgata tgtctagga gtaattatt 
      cgaccgaaa tgcctttat gtgaaatat tcccgtcgt 
      tttccctgg ttgaccagg ttatagacg tcgataatt 
      tggtcacgc ggctgttga aagtcataa gaataatcc 
      aactggaaa acgaagact gcactaacg aactgcagg 
      agcagagag acaggcttt ccgaacaag tcgacaagc 
      aaaaagaga cgaacaaac tcgtaagca agcaactga 
      aagactggg aaatactcg gagcgcggc taaagactg 
      aagttaaag aactgatgc accagttga gcaagaaaa 
      acaacgaaa tggacagca ttaagtgcg aacttaata 
      ggcaaagag aggaagtcg agaaactga accaagatg 
      tgtgcgaac tgtcaacgg ccctagcca aggccgagc 
      ttgagatga agatgcgag acgaagaaa tatccgaag 
      cgctcaaaa actgggaag aaaacaacg aaataccac 
      ctcctgaag taatcgcgc gactaaacg cggctcaga 
      ctgaagtcc ctcttttga aagagaaac tatcgcaaa 
      aagaacgac atttgagtg agctcactc aagaattcc 
      tcgccagta gacaagtcc tgactgaaa gcttgaaag 
      aagcggtaa acgaggcga aaaagcagt acgaagcga 
      ttgacaatg ctttagagg ttctccaca acaatcaaa 
      acgtggtgc aacagtgca caccgttgg ccgagctgc 
      agagggaat tggaaaata cgaacttcc aaagtgcgt 
      ccgcaatgc cgttggcgg caccagccg attgcaacg 
      caaatgcgg ctcttttgc aagcgattg ctaatttac 
      agatcaaca ccactgcct aagattgtg aaagtcaca 
      atgatagtg atgccattg attttgact atgatttgt 
      acataaatc ggtttttat ttaagtttt tcctagaga 
      tttaaaagg ctgaattat gccattcta cgtccaagt 
      gtacgaatt ttaacagta ggaggagtc gttccagac 
      tgttggacc cgttgcagt taaacagac actaaggct 
      ttcttcact tttacaaaa tttttacgt catttgttt 
      gaaatcggt gcttttgat aaatccata tttttagat 
      tgtactatt atgccattg ttgtaaata aataaatta 
      cgtagacct gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY