Status
Please wait ...
Gene information
General information
TC012381 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5897747..5899869
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012381
    iBeetle screen iB_05229

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012381 RA
      MGKGFNNYM CKKFFHPAS RDNLKRVWM AEQKAEAYK 
      KKQEELRQQ YEKEQDLHD NKAMLSKES KDKLSINFM 
      YEPPPGARK EREREDNEP EYKFEWQRK YNAPRESYC 
      KGDQEIRDQ PFGIQVRNV RCIKCHKWG HINTDKECP 
      MYSLSTSVA RSLESASIL DQKSLVEQM REDGLAIKK 
      QYLSNTGTA NYDLIINED ADENSVVTF IKSLTKKQK 
      KALLKKLEK IEKGGRDKK NKKRRDSDE ASSDEDRKK 
      AGKRKFRRR NQSTSSEEE SEDDKKKHK KKEKSSEFK 
      LMSKEEKKA LIKKLEIVE KAISKRNEK NGRRSSDED 
      RSRRDKKHS RHNNDDYRR DRKRKSDDS YYENSKRRR 
      S
    CDS sequence
    • >TC012381 RA
      atgggcaaa ggcttcaac aactacatg tgtaagaag 
      ttcttccac cccgcttcg cgggacaac ctgaagcgg 
      gtgtggatg gcggagcag aaggccgag gcctacaag 
      aagaagcag gaggagctg cgccagcag tacgagaag 
      gagcaggac ttgcacgac aataaggcg atgctgagc 
      aaggagagc aaagacaag ttgagcatc aacttcatg 
      tacgagccc ccgcccggg gcgcgcaag gagcgcgag 
      cgcgaggac aacgagccg gagtacaag ttcgagtgg 
      cagcgtaaa tacaacgcc ccccgtgag agctactgt 
      aagggcgat caagagatt agggaccag ccgttcggg 
      atacaagtg cggaacgtc aggtgcatc aagtgccac 
      aaatggggg cacattaac acggataag gagtgcccc 
      atgtacagt ttgtcgacc agtgtggcc aggtcgctc 
      gagtcggcc tcgattttg gaccagaag tcgttggtt 
      gagcaaatg agggaggat gggcttgcg attaaaaag 
      cagtatttg agcaatacg gggactgct aattatgat 
      ttgattatt aacgaggat gctgatgag aattcggtt 
      gtgactttt attaagtcg ttgacgaag aagcagaag 
      aaggcgttg ttgaagaag ttggagaag attgaaaag 
      ggggggaga gataagaag aacaagaaa cgaagggat 
      tcagatgag gcttcctca gatgaagac aggaagaaa 
      gcagggaag aggaagttc aggaggagg aatcagagt 
      acgagctct gaggaggaa tcggaagat gataagaag 
      aaacacaag aagaaggaa aagtcgtca gagtttaaa 
      ctaatgtcc aaagaggag aaaaaggca ttgattaag 
      aaactggaa attgtggag aaagcaatc agtaaaagg 
      aacgaaaag aatgggcga agaagttcc gatgaggac 
      aggtcaagg agggacaaa aagcactca cggcataac 
      aatgatgac tacagacgg gacaggaag cgaaaatct 
      gatgattcc tattatgaa aatagcaaa agaagacgc 
      tcttaa
    mRNA sequence
    • >TC012381 RA
      tcagtgtgg tgtcagtgt gagtttgtt tggcgttat 
      taatcgttt atttgtgca atagtttag caaaaacag 
      tcaggatgg gcaaaggct tcaacaact acatgtgta 
      agaagttct tccaccccg cttcgcggg acaacctga 
      agcgggtgt ggatggcgg agcagaagg ccgaggcct 
      acaagaaga agcaggagg agctgcgcc agcagtacg 
      agaaggagc aggacttgc acgacaata aggcgatgc 
      tgagcaagg agagcaaag acaagttga gcatcaact 
      tcatgtacg agcccccgc ccggggcgc gcaaggagc 
      gcgagcgcg aggacaacg agccggagt acaagttcg 
      agtggcagc gtaaataca acgcccccc gtgagagct 
      actgtaagg gcgatcaag agattaggg accagccgt 
      tcgggatac aagtgcgga acgtcaggt gcatcaagt 
      gccacaaat gggggcaca ttaacacgg ataaggagt 
      gccccatgt acagtttgt cgaccagtg tggccaggt 
      cgctcgagt cggcctcga ttttggacc agaagtcgt 
      tggttgagc aaatgaggg aggatgggc ttgcgatta 
      aaaagcagt atttgagca atacgggga ctgctaatt 
      atgatttga ttattaacg aggatgctg atgagaatt 
      cggttgtga cttttatta agtcgttga cgaagaagc 
      agaagaagg cgttgttga agaagttgg agaagattg 
      aaaaggggg ggagagata agaagaaca agaaacgaa 
      gggattcag atgaggctt cctcagatg aagacagga 
      agaaagcag ggaagagga agttcagga ggaggaatc 
      agagtacga gctctgagg aggaatcgg aagatgata 
      agaagaaac acaagaaga aggaaaagt cgtcagagt 
      ttaaactaa tgtccaaag aggagaaaa aggcattga 
      ttaagaaac tggaaattg tggagaaag caatcagta 
      aaaggaacg aaaagaatg ggcgaagaa gttccgatg 
      aggacaggt caaggaggg acaaaaagc actcacggc 
      ataacaatg atgactaca gacgggaca ggaagcgaa 
      aatctgatg attcctatt atgaaaata gcaaaagaa 
      gacgctctt aagtttttg ttgtacatt tcttagaat 
      tgttgtgat tatagggat tatcatgat acttgtaat 
      aatttagaa aacgtaata tatttttaa acgtatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY