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Gene information
General information
TC012380 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5870157..5874565
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012380
    iBeetle screen iB_05228

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012380 RA
      MNLSWSNRV IKYVLFVFN LLFVISGII MIAVGVSVK 
      SYYTEYDVF LNDSYFSLP NLLIAIGSL IFFISFFGC 
      CGAVRESWF MIFIFSILL SIIFIMEFS AGIAGYVLR 
      DKTTSFLTE ELSNSMKLY QFNEKPNPT TVMWDTIQY 
      QFECCGATK PDDWAKYVP TPSTISSTV PSSSETPPY 
      SSSSPETTP TSTTNSTSE STPSATTIA EKKRRAVAQ 
      PGTESGDLP ASCCRSNNG ATGTIKCNL NDNSSATYY 
      SRGCVEGFG DYLKDHAVT LGGVGIAIA FIQLFGVIL 
      ACHLTKQLK HGFYST
    CDS sequence
    • >TC012380 RA
      atgaatctc agctggagt aatagagtt attaaatat 
      gtgctcttc gttttcaac ctgctgttt gtgatcagt 
      gggattatt atgattgca gtgggagtt tccgtcaag 
      tcatactat acagaatat gatgtattt ttaaacgac 
      agttacttc tctttaccc aatctgttg atagccatc 
      gggtcctta attttcttc atttcgttc ttcggctgt 
      tgcggggct gtgagggaa agttggttt atgatattc 
      attttttcg attttgttg tcgattatc ttcatcatg 
      gaattttct gccggaatc gctggatat gtcttaaga 
      gacaaaaca acctcattt ttaacagag gagttatca 
      aattctatg aaactgtac caatttaat gagaagcct 
      aatcccacc acagttatg tgggacaca attcaatac 
      caatttgaa tgctgcggt gctaccaaa ccggacgat 
      tgggcaaaa tacgttccc actccttca acaatttca 
      tcaacggtt ccaagttct agtgaaaca ccaccatac 
      tcgtcaagt agtcctgaa acaacacca acatctaca 
      acaaattct acaagcgaa agtactcca tctgctaca 
      acaattgcc gaaaaaaaa cgtcgagct gttgcacaa 
      ccgggcaca gaaagtgga gacctccct gcaagctgc 
      tgccggagc aacaacggg gccacggga acaataaaa 
      tgcaaccta aatgataac tcttctgcg acttactac 
      agtcggggc tgcgtcgag ggcttcgga gactatctg 
      aaagaccat gctgtaacg ttgggaggc gtcgggatt 
      gcgattgca tttatacag ctttttggc gtaatcttg 
      gcctgccat ttgaccaag cagctgaaa catggtttt 
      tacagtacg taa
    mRNA sequence
    • >TC012380 RA
      gcacataaa caaatttcc aaccgaacg aaaatgttg 
      atgtaacgc tcaatcgca ccattcgac cagcgcctg 
      tagtaaaat gtgttccag ttttaataa tagtcgcgt 
      tgtacgtgc tcgcaggtg aaaagatta aagtatgtc 
      cacgtctgc ggattgtgt tgacacgat aatgttatc 
      agttgatcc caaataact ttatcgaaa caccttcac 
      gaaaccagt cctctgtgt gcgagacct gtgtgaaag 
      tgaaatttc gaagaaagt gtccagtta agtgtcacg 
      tgctgagca aataactca aaatgaatc tcagctgga 
      gtaatagag ttattaaat atgtgctct tcgttttca 
      acctgctgt ttgtgatca gtgggatta ttatgattg 
      cagtgggag tttccgtca agtcatact atacagaat 
      atgatgtat ttttaaacg acagttact tctctttac 
      ccaatctgt tgatagcca tcgggtcct taattttct 
      tcatttcgt tcttcggct gttgcgggg ctgtgaggg 
      aaagttggt ttatgatat tcatttttt cgattttgt 
      tgtcgatta tcttcatca tggaatttt ctgccggaa 
      tcgctggat atgtcttaa gagacaaaa caacctcat 
      ttttaacag aggagttat caaattcta tgaaactgt 
      accaattta atgagaagc ctaatccca ccacagtta 
      tgtgggaca caattcaat accaatttg aatgctgcg 
      gtgctacca aaccggacg attgggcaa aatacgttc 
      ccactcctt caacaattt catcaacgg ttccaagtt 
      ctagtgaaa caccaccat actcgtcaa gtagtcctg 
      aaacaacac caacatcta caacaaatt ctacaagcg 
      aaagtactc catctgcta caacaattg ccgaaaaaa 
      aacgtcgag ctgttgcac aaccgggca cagaaagtg 
      gagacctcc ctgcaagct gctgccgga gcaacaacg 
      gggccacgg gaacaataa aatgcaacc taaatgata 
      actcttctg cgacttact acagtcggg gctgcgtcg 
      agggcttcg gagactatc tgaaagacc atgctgtaa 
      cgttgggag gcgtcggga ttgcgattg catttatac 
      agctttttg gcgtaatct tggcctgcc atttgacca 
      agcagctga aacatggtt tttacagta cgtaaattg 
      tgcataatt aacaagata tattttcga aaggaatta 
      atgttttat tgatttatt gttacttat ttgaaatat 
      agctactaa acatagttg tttctattt ttgctaaca 
      atgttggac ttagtattt tttactgag ctagctcat 
      tgtgtgagt atcttattg tttgccatt tgctgaatt 
      ttgggtttt gcaaaattc acaactttg tttattatt 
      gttgcgtca ttgtgaaaa tattagggt tactctaaa 
      cagatcttt tattacttg ttgtttcac aaaaataga 
      atttattgc gtagtattt aaacacatg gctccctag 
      taaaagtgt tgcagcttc tttgagtat taagggcct 
      aacgaacaa attgtcttg tcacaccca tctaaaaaa 
      attgtattg aacttgttc ctaattgtg taagtgtat 
      ctattctat acatattgt tatacgact tttgaataa 
      aaattacgt cactaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY