| General information | |||
| TC012366 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 5758572..5765810 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC012366 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05226 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC012366 RA
MIAMENNNT MTFVCAELS STMFQKLYD QKKSNRFCD LTLHVNNKI VKAHRNVLA CSSPYFDSI LKHHKIIRE QLIIKCLDS EIFNTILNY MYTGEITIE HSNVEELLK LADHFIITK VIEYCIEFL GTKLNLDNC LFTYFLTQR FKLKHLGNI VENWIMSHI DDVCDGEEI IGLNVTELQ EFFKNKSFV LSTARALNV LSQWVLHNV EKRESHFDK LVRCFHTNS LEPAEVFKH LDTCILYSK SELCMYRFL DYLLQNNWM LSNFKSRYD NLHVKYGQM ISCNKNEEE DSKSDKIVV SEKTDSSSL QNSFSSVLV KAKLRNKKQ LILKRLMLF GLKPSLRMA ALKMLTSKK KVLPLADSG QEEEEDNDE KMDIKCPIC FTTINDSLL LEQHLALSH AKDVTYKCG ICSFVCQYH GDYLNHMKS HFSGPPYKC DYCDTTADQ ISKLISHRA QHLEESVYQ CTFCSFKCR LKQNFVSHL KIHTPEKNF KCDHCTKSF RFKQNLETH MLTHTTDKN LTCESCGFH TKFLSHMIA HKRIHAVFS PGDIYRCSY PHCKYSTAK KNQLASHAK SHNGVRPHS CGICGRGFM EKSHLVRHE RIHLEEKPF KCSNCDYAS SRRDKLKEH FTRHHGENA SAKVPYKAR PMRNNSTRP KSQASQEAT TTTNNNNNN NNVNFTQTN SSSTSSTGT GVTQPIGNE IQDLIMHHQ NHSATSSAT NLSAINSNY HHFGDPTDF HHHNHAHNI HNQILASGH NQRSATQHH NNNIVNHHM LARSNHSTA TSTAAAVAA AMMLDPRFH HNTSVPYHP TTTPVSMAM AAVQSSQQI QASGQHSEY PPSLQNCMT LF
- >TC012366 RA
atgattgcg atggagaac aacaacacg atgaccttt gtttgtgcg gaattatca tcgaccatg ttccagaag ctgtacgac caaaagaaa tcgaatcga ttttgcgat ttgactctg cacgtaaac aataagatc gtaaaagcg caccgtaac gtactagca tgcagttcc ccgtatttc gattcgatc ctcaagcat cacaagata attagagaa cagttgatt ataaaatgt ttagatagc gaaattttt aacacgatt ttaaactac atgtacact ggtgagatc accatagaa cactcgaac gtagaggag ctcctcaag ctagccgac catttcatc ataactaaa gtaattgaa tactgcata gagtttctg gggactaag ctgaacctg gacaactgt ctgttcacc tattttcta acgcagcgt ttcaagttg aaacacttg gggaacatc gttgagaac tggataatg agccacatt gacgacgtt tgcgacggc gaggaaata atcgggctg aacgtcacc gaactgcag gaatttttc aaaaacaaa agctttgtc ctttccact gcaagagct ttaaatgtg ctttctcaa tgggttttg cacaacgtt gaaaaacgc gaatcacac tttgataag ttggtcaga tgtttccac acgaactcg ctcgaacct gccgaggtt ttcaaacat ttggacacc tgtattctg tacagcaaa agcgagctg tgcatgtac cggtttctg gactatttg ctgcaaaat aattggatg ctgtccaat ttcaagtcg agatatgac aatctccat gttaaatac ggccagatg atttcgtgt aataaaaac gaggaagaa gactcaaag tcggacaaa atcgtcgtg tccgagaaa accgactca agctcgctg cagaacagc ttcagttca gtgttagtc aaggcgaaa ttgcgcaac aagaagcaa cttatcttg aaaagactg atgttattt ggtttaaaa cctagtcta agaatggca gctttaaaa atgttgact tcgaaaaag aaggttttg cctttggcc gattcggga caggaagaa gaagaagac aatgatgaa aagatggac atcaaatgc ccgatttgt ttcacaaca atcaatgat agtctgctg ctagagcag catttggcg ttaagtcat gccaaagat gtgacctac aagtgcggg atttgttct tttgtgtgt cagtaccac ggcgattat ttgaaccac atgaagagc cacttttcg gggccgccc tataagtgc gactactgt gacaccaca gctgatcaa atttccaag ctaatctcc catcgtgcc caacacttg gaagagtcc gtttaccag tgcaccttt tgctctttc aaatgccgc ctcaagcaa aacttcgtc tcccattta aaaatccac acccccgag aagaacttc aaatgcgac cactgcaca aaatccttc cgtttcaag caaaacctc gaaacgcac atgctgaca cacaccacc gacaaaaac ctaacgtgc gaatcgtgc ggtttccac acgaaattc ctcagccac atgatcgcc cacaaacgc atccacgca gttttttct ccaggggac atctaccgc tgttcgtac ccccattgt aagtactca acggcgaaa aagaaccaa ttggcaagt catgccaag agccacaat ggggtcagg ccgcattcg tgcgggatt tgcgggagg ggcttcatg gagaagtcg cacttggtg cgacacgag cgcatccac ttggaggag aagcccttc aagtgctcg aactgcgat tacgcgagc tcgaggcgg gataaactg aaggagcat tttacaaga caccatgga gagaacgcc tcggcgaag gtgccctac aaggcccga ccaatgcgc aacaactcc acacggccc aagtcacaa gcttcgcag gaagccacc accactacg aataacaac aataataat aacaacgta aatttcacg caaactaat agtagtagc actagtagt acagggacc ggagtaacc caaccaata ggcaacgaa atacaagac ttgatcatg catcaccag aatcattca gccacgtct tctgctacg aatttatcg gccattaat tcgaattat caccatttc ggcgatccc accgatttt catcatcat aaccacgca cataatata cataatcag attttggcg tctggacat aatcaaaga tcggccaca caacatcat aataacaat attgttaat catcatatg ttggcaagg tctaatcat tccacggcc acgtccacc gctgctgct gtcgctgct gctatgatg cttgaccca aggtttcac cacaatact tccgtgccc taccaccca accacaacc ccggtctca atggcaatg gctgctgtt caatcatca caacagatc caagcttcg ggccaacac tccgaatat cctccttct ctccagaac tgcatgaca ctattctag
- >TC012366 RA
atcagtctg tcaatcaac tcaaaatgg tttggtgca gtgttccta attattgca atttttttg caacaataa gacgataat agtgagata aaatgtgaa aacgggcac tacattggg ggttcgttg cagcatgat tgcgatgga gaacaacaa cacgatgac ctttgtttg tgcggaatt atcatcgac catgttcca gaagctgta cgaccaaaa gaaatcgaa tcgattttg cgatttgac tctgcacgt aaacaataa gatcgtaaa agcgcaccg taacgtact agcatgcag ttccccgta tttcgattc gatcctcaa gcatcacaa gataattag agaacagtt gattataaa atgtttaga tagcgaaat ttttaacac gattttaaa ctacatgta cactggtga gatcaccat agaacactc gaacgtaga ggagctcct caagctagc cgaccattt catcataac taaagtaat tgaatactg catagagtt tctggggac taagctgaa cctggacaa ctgtctgtt cacctattt tctaacgca gcgtttcaa gttgaaaca cttggggaa catcgttga gaactggat aatgagcca cattgacga cgtttgcga cggcgagga aataatcgg gctgaacgt caccgaact gcaggaatt tttcaaaaa caaaagctt tgtcctttc cactgcaag agctttaaa tgtgctttc tcaatgggt tttgcacaa cgttgaaaa acgcgaatc acactttga taagttggt cagatgttt ccacacgaa ctcgctcga acctgccga ggttttcaa acatttgga cacctgtat tctgtacag caaaagcga gctgtgcat gtaccggtt tctggacta tttgctgca aaataattg gatgctgtc caatttcaa gtcgagata tgacaatct ccatgttaa atacggcca gatgatttc gtgtaataa aaacgagga agaagactc aaagtcgga caaaatcgt cgtgtccga gaaaaccga ctcaagctc gctgcagaa cagcttcag ttcagtgtt agtcaaggc gaaattgcg caacaagaa gcaacttat cttgaaaag actgatgtt atttggttt aaaacctag tctaagaat ggcagcttt aaaaatgtt gacttcgaa aaagaaggt tttgccttt ggccgattc gggacagga agaagaaga agacaatga tgaaaagat ggacatcaa atgcccgat ttgtttcac aacaatcaa tgatagtct gctgctaga gcagcattt ggcgttaag tcatgccaa agatgtgac ctacaagtg cgggatttg ttcttttgt gtgtcagta ccacggcga ttatttgaa ccacatgaa gagccactt ttcggggcc gccctataa gtgcgacta ctgtgacac cacagctga tcaaatttc caagctaat ctcccatcg tgcccaaca cttggaaga gtccgttta ccagtgcac cttttgctc tttcaaatg ccgcctcaa gcaaaactt cgtctccca tttaaaaat ccacacccc cgagaagaa cttcaaatg cgaccactg cacaaaatc cttccgttt caagcaaaa cctcgaaac gcacatgct gacacacac caccgacaa aaacctaac gtgcgaatc gtgcggttt ccacacgaa attcctcag ccacatgat cgcccacaa acgcatcca cgcagtttt ttctccagg ggacatcta ccgctgttc gtaccccca ttgtaagta ctcaacggc gaaaaagaa ccaattggc aagtcatgc caagagcca caatggggt caggccgca ttcgtgcgg gatttgcgg gaggggctt catggagaa gtcgcactt ggtgcgaca cgagcgcat ccacttgga ggagaagcc cttcaagtg ctcgaactg cgattacgc gagctcgag gcgggataa actgaagga gcattttac aagacacca tggagagaa cgcctcggc gaaggtgcc ctacaaggc ccgaccaat gcgcaacaa ctccacacg gcccaagtc acaagcttc gcaggaagc caccaccac tacgaataa caacaataa taataacaa cgtaaattt cacgcaaac taatagtag tagcactag tagtacagg gaccggagt aacccaacc aataggcaa cgaaataca agacttgat catgcatca ccagaatca ttcagccac gtcttctgc tacgaattt atcggccat taattcgaa ttatcacca tttcggcga tcccaccga ttttcatca tcataacca cgcacataa tatacataa tcagatttt ggcgtctgg acataatca aagatcggc cacacaaca tcataataa caatattgt taatcatca tatgttggc aaggtctaa tcattccac ggccacgtc caccgctgc tgctgtcgc tgctgctat gatgcttga cccaaggtt tcaccacaa tacttccgt gccctacca cccaaccac aaccccggt ctcaatggc aatggctgc tgttcaatc atcacaaca gatccaagc ttcgggcca acactccga atatcctcc ttctctcca gaactgcat gacactatt ctagtctga ataattgtg attcctatt ttgttatct agtatttaa tcggcattt gttgatgtt gtaactttt ttgttgact tattagcga atttctgtt gatttgttt tcatagttt ttatattat tataatgca ctaaaaggt aataaacag taatgtgta tc
