Status
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Gene information
General information
TC012366 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5758572..5765810
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012366
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05226

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012366 RA
      MIAMENNNT MTFVCAELS STMFQKLYD QKKSNRFCD 
      LTLHVNNKI VKAHRNVLA CSSPYFDSI LKHHKIIRE 
      QLIIKCLDS EIFNTILNY MYTGEITIE HSNVEELLK 
      LADHFIITK VIEYCIEFL GTKLNLDNC LFTYFLTQR 
      FKLKHLGNI VENWIMSHI DDVCDGEEI IGLNVTELQ 
      EFFKNKSFV LSTARALNV LSQWVLHNV EKRESHFDK 
      LVRCFHTNS LEPAEVFKH LDTCILYSK SELCMYRFL 
      DYLLQNNWM LSNFKSRYD NLHVKYGQM ISCNKNEEE 
      DSKSDKIVV SEKTDSSSL QNSFSSVLV KAKLRNKKQ 
      LILKRLMLF GLKPSLRMA ALKMLTSKK KVLPLADSG 
      QEEEEDNDE KMDIKCPIC FTTINDSLL LEQHLALSH 
      AKDVTYKCG ICSFVCQYH GDYLNHMKS HFSGPPYKC 
      DYCDTTADQ ISKLISHRA QHLEESVYQ CTFCSFKCR 
      LKQNFVSHL KIHTPEKNF KCDHCTKSF RFKQNLETH 
      MLTHTTDKN LTCESCGFH TKFLSHMIA HKRIHAVFS 
      PGDIYRCSY PHCKYSTAK KNQLASHAK SHNGVRPHS 
      CGICGRGFM EKSHLVRHE RIHLEEKPF KCSNCDYAS 
      SRRDKLKEH FTRHHGENA SAKVPYKAR PMRNNSTRP 
      KSQASQEAT TTTNNNNNN NNVNFTQTN SSSTSSTGT 
      GVTQPIGNE IQDLIMHHQ NHSATSSAT NLSAINSNY 
      HHFGDPTDF HHHNHAHNI HNQILASGH NQRSATQHH 
      NNNIVNHHM LARSNHSTA TSTAAAVAA AMMLDPRFH 
      HNTSVPYHP TTTPVSMAM AAVQSSQQI QASGQHSEY 
      PPSLQNCMT LF
    CDS sequence
    • >TC012366 RA
      atgattgcg atggagaac aacaacacg atgaccttt 
      gtttgtgcg gaattatca tcgaccatg ttccagaag 
      ctgtacgac caaaagaaa tcgaatcga ttttgcgat 
      ttgactctg cacgtaaac aataagatc gtaaaagcg 
      caccgtaac gtactagca tgcagttcc ccgtatttc 
      gattcgatc ctcaagcat cacaagata attagagaa 
      cagttgatt ataaaatgt ttagatagc gaaattttt 
      aacacgatt ttaaactac atgtacact ggtgagatc 
      accatagaa cactcgaac gtagaggag ctcctcaag 
      ctagccgac catttcatc ataactaaa gtaattgaa 
      tactgcata gagtttctg gggactaag ctgaacctg 
      gacaactgt ctgttcacc tattttcta acgcagcgt 
      ttcaagttg aaacacttg gggaacatc gttgagaac 
      tggataatg agccacatt gacgacgtt tgcgacggc 
      gaggaaata atcgggctg aacgtcacc gaactgcag 
      gaatttttc aaaaacaaa agctttgtc ctttccact 
      gcaagagct ttaaatgtg ctttctcaa tgggttttg 
      cacaacgtt gaaaaacgc gaatcacac tttgataag 
      ttggtcaga tgtttccac acgaactcg ctcgaacct 
      gccgaggtt ttcaaacat ttggacacc tgtattctg 
      tacagcaaa agcgagctg tgcatgtac cggtttctg 
      gactatttg ctgcaaaat aattggatg ctgtccaat 
      ttcaagtcg agatatgac aatctccat gttaaatac 
      ggccagatg atttcgtgt aataaaaac gaggaagaa 
      gactcaaag tcggacaaa atcgtcgtg tccgagaaa 
      accgactca agctcgctg cagaacagc ttcagttca 
      gtgttagtc aaggcgaaa ttgcgcaac aagaagcaa 
      cttatcttg aaaagactg atgttattt ggtttaaaa 
      cctagtcta agaatggca gctttaaaa atgttgact 
      tcgaaaaag aaggttttg cctttggcc gattcggga 
      caggaagaa gaagaagac aatgatgaa aagatggac 
      atcaaatgc ccgatttgt ttcacaaca atcaatgat 
      agtctgctg ctagagcag catttggcg ttaagtcat 
      gccaaagat gtgacctac aagtgcggg atttgttct 
      tttgtgtgt cagtaccac ggcgattat ttgaaccac 
      atgaagagc cacttttcg gggccgccc tataagtgc 
      gactactgt gacaccaca gctgatcaa atttccaag 
      ctaatctcc catcgtgcc caacacttg gaagagtcc 
      gtttaccag tgcaccttt tgctctttc aaatgccgc 
      ctcaagcaa aacttcgtc tcccattta aaaatccac 
      acccccgag aagaacttc aaatgcgac cactgcaca 
      aaatccttc cgtttcaag caaaacctc gaaacgcac 
      atgctgaca cacaccacc gacaaaaac ctaacgtgc 
      gaatcgtgc ggtttccac acgaaattc ctcagccac 
      atgatcgcc cacaaacgc atccacgca gttttttct 
      ccaggggac atctaccgc tgttcgtac ccccattgt 
      aagtactca acggcgaaa aagaaccaa ttggcaagt 
      catgccaag agccacaat ggggtcagg ccgcattcg 
      tgcgggatt tgcgggagg ggcttcatg gagaagtcg 
      cacttggtg cgacacgag cgcatccac ttggaggag 
      aagcccttc aagtgctcg aactgcgat tacgcgagc 
      tcgaggcgg gataaactg aaggagcat tttacaaga 
      caccatgga gagaacgcc tcggcgaag gtgccctac 
      aaggcccga ccaatgcgc aacaactcc acacggccc 
      aagtcacaa gcttcgcag gaagccacc accactacg 
      aataacaac aataataat aacaacgta aatttcacg 
      caaactaat agtagtagc actagtagt acagggacc 
      ggagtaacc caaccaata ggcaacgaa atacaagac 
      ttgatcatg catcaccag aatcattca gccacgtct 
      tctgctacg aatttatcg gccattaat tcgaattat 
      caccatttc ggcgatccc accgatttt catcatcat 
      aaccacgca cataatata cataatcag attttggcg 
      tctggacat aatcaaaga tcggccaca caacatcat 
      aataacaat attgttaat catcatatg ttggcaagg 
      tctaatcat tccacggcc acgtccacc gctgctgct 
      gtcgctgct gctatgatg cttgaccca aggtttcac 
      cacaatact tccgtgccc taccaccca accacaacc 
      ccggtctca atggcaatg gctgctgtt caatcatca 
      caacagatc caagcttcg ggccaacac tccgaatat 
      cctccttct ctccagaac tgcatgaca ctattctag
    mRNA sequence
    • >TC012366 RA
      atcagtctg tcaatcaac tcaaaatgg tttggtgca 
      gtgttccta attattgca atttttttg caacaataa 
      gacgataat agtgagata aaatgtgaa aacgggcac 
      tacattggg ggttcgttg cagcatgat tgcgatgga 
      gaacaacaa cacgatgac ctttgtttg tgcggaatt 
      atcatcgac catgttcca gaagctgta cgaccaaaa 
      gaaatcgaa tcgattttg cgatttgac tctgcacgt 
      aaacaataa gatcgtaaa agcgcaccg taacgtact 
      agcatgcag ttccccgta tttcgattc gatcctcaa 
      gcatcacaa gataattag agaacagtt gattataaa 
      atgtttaga tagcgaaat ttttaacac gattttaaa 
      ctacatgta cactggtga gatcaccat agaacactc 
      gaacgtaga ggagctcct caagctagc cgaccattt 
      catcataac taaagtaat tgaatactg catagagtt 
      tctggggac taagctgaa cctggacaa ctgtctgtt 
      cacctattt tctaacgca gcgtttcaa gttgaaaca 
      cttggggaa catcgttga gaactggat aatgagcca 
      cattgacga cgtttgcga cggcgagga aataatcgg 
      gctgaacgt caccgaact gcaggaatt tttcaaaaa 
      caaaagctt tgtcctttc cactgcaag agctttaaa 
      tgtgctttc tcaatgggt tttgcacaa cgttgaaaa 
      acgcgaatc acactttga taagttggt cagatgttt 
      ccacacgaa ctcgctcga acctgccga ggttttcaa 
      acatttgga cacctgtat tctgtacag caaaagcga 
      gctgtgcat gtaccggtt tctggacta tttgctgca 
      aaataattg gatgctgtc caatttcaa gtcgagata 
      tgacaatct ccatgttaa atacggcca gatgatttc 
      gtgtaataa aaacgagga agaagactc aaagtcgga 
      caaaatcgt cgtgtccga gaaaaccga ctcaagctc 
      gctgcagaa cagcttcag ttcagtgtt agtcaaggc 
      gaaattgcg caacaagaa gcaacttat cttgaaaag 
      actgatgtt atttggttt aaaacctag tctaagaat 
      ggcagcttt aaaaatgtt gacttcgaa aaagaaggt 
      tttgccttt ggccgattc gggacagga agaagaaga 
      agacaatga tgaaaagat ggacatcaa atgcccgat 
      ttgtttcac aacaatcaa tgatagtct gctgctaga 
      gcagcattt ggcgttaag tcatgccaa agatgtgac 
      ctacaagtg cgggatttg ttcttttgt gtgtcagta 
      ccacggcga ttatttgaa ccacatgaa gagccactt 
      ttcggggcc gccctataa gtgcgacta ctgtgacac 
      cacagctga tcaaatttc caagctaat ctcccatcg 
      tgcccaaca cttggaaga gtccgttta ccagtgcac 
      cttttgctc tttcaaatg ccgcctcaa gcaaaactt 
      cgtctccca tttaaaaat ccacacccc cgagaagaa 
      cttcaaatg cgaccactg cacaaaatc cttccgttt 
      caagcaaaa cctcgaaac gcacatgct gacacacac 
      caccgacaa aaacctaac gtgcgaatc gtgcggttt 
      ccacacgaa attcctcag ccacatgat cgcccacaa 
      acgcatcca cgcagtttt ttctccagg ggacatcta 
      ccgctgttc gtaccccca ttgtaagta ctcaacggc 
      gaaaaagaa ccaattggc aagtcatgc caagagcca 
      caatggggt caggccgca ttcgtgcgg gatttgcgg 
      gaggggctt catggagaa gtcgcactt ggtgcgaca 
      cgagcgcat ccacttgga ggagaagcc cttcaagtg 
      ctcgaactg cgattacgc gagctcgag gcgggataa 
      actgaagga gcattttac aagacacca tggagagaa 
      cgcctcggc gaaggtgcc ctacaaggc ccgaccaat 
      gcgcaacaa ctccacacg gcccaagtc acaagcttc 
      gcaggaagc caccaccac tacgaataa caacaataa 
      taataacaa cgtaaattt cacgcaaac taatagtag 
      tagcactag tagtacagg gaccggagt aacccaacc 
      aataggcaa cgaaataca agacttgat catgcatca 
      ccagaatca ttcagccac gtcttctgc tacgaattt 
      atcggccat taattcgaa ttatcacca tttcggcga 
      tcccaccga ttttcatca tcataacca cgcacataa 
      tatacataa tcagatttt ggcgtctgg acataatca 
      aagatcggc cacacaaca tcataataa caatattgt 
      taatcatca tatgttggc aaggtctaa tcattccac 
      ggccacgtc caccgctgc tgctgtcgc tgctgctat 
      gatgcttga cccaaggtt tcaccacaa tacttccgt 
      gccctacca cccaaccac aaccccggt ctcaatggc 
      aatggctgc tgttcaatc atcacaaca gatccaagc 
      ttcgggcca acactccga atatcctcc ttctctcca 
      gaactgcat gacactatt ctagtctga ataattgtg 
      attcctatt ttgttatct agtatttaa tcggcattt 
      gttgatgtt gtaactttt ttgttgact tattagcga 
      atttctgtt gatttgttt tcatagttt ttatattat 
      tataatgca ctaaaaggt aataaacag taatgtgta 
      tc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY