Status
Please wait ...
Gene information
General information
TC012366 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5758572..5765810
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC012366
    iBeetle screen iB_05226

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012366 RA
      MIAMENNNT MTFVCAELS STMFQKLYD QKKSNRFCD 
LTLHVNNKI VKAHRNVLA CSSPYFDSI LKHHKIIRE 
QLIIKCLDS EIFNTILNY MYTGEITIE HSNVEELLK 
LADHFIITK VIEYCIEFL GTKLNLDNC LFTYFLTQR 
FKLKHLGNI VENWIMSHI DDVCDGEEI IGLNVTELQ 
EFFKNKSFV LSTARALNV LSQWVLHNV EKRESHFDK 
LVRCFHTNS LEPAEVFKH LDTCILYSK SELCMYRFL 
DYLLQNNWM LSNFKSRYD NLHVKYGQM ISCNKNEEE 
DSKSDKIVV SEKTDSSSL QNSFSSVLV KAKLRNKKQ 
LILKRLMLF GLKPSLRMA ALKMLTSKK KVLPLADSG 
QEEEEDNDE KMDIKCPIC FTTINDSLL LEQHLALSH 
AKDVTYKCG ICSFVCQYH GDYLNHMKS HFSGPPYKC 
DYCDTTADQ ISKLISHRA QHLEESVYQ CTFCSFKCR 
LKQNFVSHL KIHTPEKNF KCDHCTKSF RFKQNLETH 
MLTHTTDKN LTCESCGFH TKFLSHMIA HKRIHAVFS 
PGDIYRCSY PHCKYSTAK KNQLASHAK SHNGVRPHS 
CGICGRGFM EKSHLVRHE RIHLEEKPF KCSNCDYAS 
SRRDKLKEH FTRHHGENA SAKVPYKAR PMRNNSTRP 
KSQASQEAT TTTNNNNNN NNVNFTQTN SSSTSSTGT 
GVTQPIGNE IQDLIMHHQ NHSATSSAT NLSAINSNY 
HHFGDPTDF HHHNHAHNI HNQILASGH NQRSATQHH 
NNNIVNHHM LARSNHSTA TSTAAAVAA AMMLDPRFH 
HNTSVPYHP TTTPVSMAM AAVQSSQQI QASGQHSEY 
PPSLQNCMT LF
    CDS sequence
    • >TC012366 RA
      atgattgcg atggagaac aacaacacg atgaccttt 
gtttgtgcg gaattatca tcgaccatg ttccagaag 
ctgtacgac caaaagaaa tcgaatcga ttttgcgat 
ttgactctg cacgtaaac aataagatc gtaaaagcg 
caccgtaac gtactagca tgcagttcc ccgtatttc 
gattcgatc ctcaagcat cacaagata attagagaa 
cagttgatt ataaaatgt ttagatagc gaaattttt 
aacacgatt ttaaactac atgtacact ggtgagatc 
accatagaa cactcgaac gtagaggag ctcctcaag 
ctagccgac catttcatc ataactaaa gtaattgaa 
tactgcata gagtttctg gggactaag ctgaacctg 
gacaactgt ctgttcacc tattttcta acgcagcgt 
ttcaagttg aaacacttg gggaacatc gttgagaac 
tggataatg agccacatt gacgacgtt tgcgacggc 
gaggaaata atcgggctg aacgtcacc gaactgcag 
gaatttttc aaaaacaaa agctttgtc ctttccact 
gcaagagct ttaaatgtg ctttctcaa tgggttttg 
cacaacgtt gaaaaacgc gaatcacac tttgataag 
ttggtcaga tgtttccac acgaactcg ctcgaacct 
gccgaggtt ttcaaacat ttggacacc tgtattctg 
tacagcaaa agcgagctg tgcatgtac cggtttctg 
gactatttg ctgcaaaat aattggatg ctgtccaat 
ttcaagtcg agatatgac aatctccat gttaaatac 
ggccagatg atttcgtgt aataaaaac gaggaagaa 
gactcaaag tcggacaaa atcgtcgtg tccgagaaa 
accgactca agctcgctg cagaacagc ttcagttca 
gtgttagtc aaggcgaaa ttgcgcaac aagaagcaa 
cttatcttg aaaagactg atgttattt ggtttaaaa 
cctagtcta agaatggca gctttaaaa atgttgact 
tcgaaaaag aaggttttg cctttggcc gattcggga 
caggaagaa gaagaagac aatgatgaa aagatggac 
atcaaatgc ccgatttgt ttcacaaca atcaatgat 
agtctgctg ctagagcag catttggcg ttaagtcat 
gccaaagat gtgacctac aagtgcggg atttgttct 
tttgtgtgt cagtaccac ggcgattat ttgaaccac 
atgaagagc cacttttcg gggccgccc tataagtgc 
gactactgt gacaccaca gctgatcaa atttccaag 
ctaatctcc catcgtgcc caacacttg gaagagtcc 
gtttaccag tgcaccttt tgctctttc aaatgccgc 
ctcaagcaa aacttcgtc tcccattta aaaatccac 
acccccgag aagaacttc aaatgcgac cactgcaca 
aaatccttc cgtttcaag caaaacctc gaaacgcac 
atgctgaca cacaccacc gacaaaaac ctaacgtgc 
gaatcgtgc ggtttccac acgaaattc ctcagccac 
atgatcgcc cacaaacgc atccacgca gttttttct 
ccaggggac atctaccgc tgttcgtac ccccattgt 
aagtactca acggcgaaa aagaaccaa ttggcaagt 
catgccaag agccacaat ggggtcagg ccgcattcg 
tgcgggatt tgcgggagg ggcttcatg gagaagtcg 
cacttggtg cgacacgag cgcatccac ttggaggag 
aagcccttc aagtgctcg aactgcgat tacgcgagc 
tcgaggcgg gataaactg aaggagcat tttacaaga 
caccatgga gagaacgcc tcggcgaag gtgccctac 
aaggcccga ccaatgcgc aacaactcc acacggccc 
aagtcacaa gcttcgcag gaagccacc accactacg 
aataacaac aataataat aacaacgta aatttcacg 
caaactaat agtagtagc actagtagt acagggacc 
ggagtaacc caaccaata ggcaacgaa atacaagac 
ttgatcatg catcaccag aatcattca gccacgtct 
tctgctacg aatttatcg gccattaat tcgaattat 
caccatttc ggcgatccc accgatttt catcatcat 
aaccacgca cataatata cataatcag attttggcg 
tctggacat aatcaaaga tcggccaca caacatcat 
aataacaat attgttaat catcatatg ttggcaagg 
tctaatcat tccacggcc acgtccacc gctgctgct 
gtcgctgct gctatgatg cttgaccca aggtttcac 
cacaatact tccgtgccc taccaccca accacaacc 
ccggtctca atggcaatg gctgctgtt caatcatca 
caacagatc caagcttcg ggccaacac tccgaatat 
cctccttct ctccagaac tgcatgaca ctattctag
    mRNA sequence
    • >TC012366 RA
      atcagtctg tcaatcaac tcaaaatgg tttggtgca 
gtgttccta attattgca atttttttg caacaataa 
gacgataat agtgagata aaatgtgaa aacgggcac 
tacattggg ggttcgttg cagcatgat tgcgatgga 
gaacaacaa cacgatgac ctttgtttg tgcggaatt 
atcatcgac catgttcca gaagctgta cgaccaaaa 
gaaatcgaa tcgattttg cgatttgac tctgcacgt 
aaacaataa gatcgtaaa agcgcaccg taacgtact 
agcatgcag ttccccgta tttcgattc gatcctcaa 
gcatcacaa gataattag agaacagtt gattataaa 
atgtttaga tagcgaaat ttttaacac gattttaaa 
ctacatgta cactggtga gatcaccat agaacactc 
gaacgtaga ggagctcct caagctagc cgaccattt 
catcataac taaagtaat tgaatactg catagagtt 
tctggggac taagctgaa cctggacaa ctgtctgtt 
cacctattt tctaacgca gcgtttcaa gttgaaaca 
cttggggaa catcgttga gaactggat aatgagcca 
cattgacga cgtttgcga cggcgagga aataatcgg 
gctgaacgt caccgaact gcaggaatt tttcaaaaa 
caaaagctt tgtcctttc cactgcaag agctttaaa 
tgtgctttc tcaatgggt tttgcacaa cgttgaaaa 
acgcgaatc acactttga taagttggt cagatgttt 
ccacacgaa ctcgctcga acctgccga ggttttcaa 
acatttgga cacctgtat tctgtacag caaaagcga 
gctgtgcat gtaccggtt tctggacta tttgctgca 
aaataattg gatgctgtc caatttcaa gtcgagata 
tgacaatct ccatgttaa atacggcca gatgatttc 
gtgtaataa aaacgagga agaagactc aaagtcgga 
caaaatcgt cgtgtccga gaaaaccga ctcaagctc 
gctgcagaa cagcttcag ttcagtgtt agtcaaggc 
gaaattgcg caacaagaa gcaacttat cttgaaaag 
actgatgtt atttggttt aaaacctag tctaagaat 
ggcagcttt aaaaatgtt gacttcgaa aaagaaggt 
tttgccttt ggccgattc gggacagga agaagaaga 
agacaatga tgaaaagat ggacatcaa atgcccgat 
ttgtttcac aacaatcaa tgatagtct gctgctaga 
gcagcattt ggcgttaag tcatgccaa agatgtgac 
ctacaagtg cgggatttg ttcttttgt gtgtcagta 
ccacggcga ttatttgaa ccacatgaa gagccactt 
ttcggggcc gccctataa gtgcgacta ctgtgacac 
cacagctga tcaaatttc caagctaat ctcccatcg 
tgcccaaca cttggaaga gtccgttta ccagtgcac 
cttttgctc tttcaaatg ccgcctcaa gcaaaactt 
cgtctccca tttaaaaat ccacacccc cgagaagaa 
cttcaaatg cgaccactg cacaaaatc cttccgttt 
caagcaaaa cctcgaaac gcacatgct gacacacac 
caccgacaa aaacctaac gtgcgaatc gtgcggttt 
ccacacgaa attcctcag ccacatgat cgcccacaa 
acgcatcca cgcagtttt ttctccagg ggacatcta 
ccgctgttc gtaccccca ttgtaagta ctcaacggc 
gaaaaagaa ccaattggc aagtcatgc caagagcca 
caatggggt caggccgca ttcgtgcgg gatttgcgg 
gaggggctt catggagaa gtcgcactt ggtgcgaca 
cgagcgcat ccacttgga ggagaagcc cttcaagtg 
ctcgaactg cgattacgc gagctcgag gcgggataa 
actgaagga gcattttac aagacacca tggagagaa 
cgcctcggc gaaggtgcc ctacaaggc ccgaccaat 
gcgcaacaa ctccacacg gcccaagtc acaagcttc 
gcaggaagc caccaccac tacgaataa caacaataa 
taataacaa cgtaaattt cacgcaaac taatagtag 
tagcactag tagtacagg gaccggagt aacccaacc 
aataggcaa cgaaataca agacttgat catgcatca 
ccagaatca ttcagccac gtcttctgc tacgaattt 
atcggccat taattcgaa ttatcacca tttcggcga 
tcccaccga ttttcatca tcataacca cgcacataa 
tatacataa tcagatttt ggcgtctgg acataatca 
aagatcggc cacacaaca tcataataa caatattgt 
taatcatca tatgttggc aaggtctaa tcattccac 
ggccacgtc caccgctgc tgctgtcgc tgctgctat 
gatgcttga cccaaggtt tcaccacaa tacttccgt 
gccctacca cccaaccac aaccccggt ctcaatggc 
aatggctgc tgttcaatc atcacaaca gatccaagc 
ttcgggcca acactccga atatcctcc ttctctcca 
gaactgcat gacactatt ctagtctga ataattgtg 
attcctatt ttgttatct agtatttaa tcggcattt 
gttgatgtt gtaactttt ttgttgact tattagcga 
atttctgtt gatttgttt tcatagttt ttatattat 
tataatgca ctaaaaggt aataaacag taatgtgta 
tc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY