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Gene information
General information
TC012353 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5482490..5490940
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012353
    iBeetle screen iB_05223

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva dorsal ventralpattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    prothoracic leg partially fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Femur and Tibiatarus are mainly affected.  Specifically, Only one leg of prothorax is affected.
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012353 RA
      MNEIVSQVQ SGSFDLIEL FNERTNEIA EIKAAVKRL 
      TSTLPRDKC IDVFVTKLV PAMFAKNVD DWIRDELIE 
      LGSHFVQFV TVSTLDTKD QEEVFAAID NTYIKVVGQ 
      LRDSNYPHW DQLWEFLIK LSNEKLHSR QGTGNKLLK 
      EVEVAFRNS SSTEQRLKA YDCWKELIN NSSLNMEYM 
      CSAKQLKLL VTPMKAKFS RTPTVIIKR CDTYIYLLE 
      KLQDKATLC LKEFLEFCF GPFDEKNDS ERTRQGRSV 
      PEVCCKSTK VLLEIIGHS HDPAAKCLK SDEFQLFKP 
      LITNENFPS FAEILINSV MECCTLLHH CQQNKIINR 
      VIIQCLWQS LLRLIHQSS DEVKKKCCA QINSLLNKV 
      LRHSGQSEF LYLVLGNIV ELLVRDKLV DEFKDIVFA 
      IAVHLLWMI NFQTCAFLE FYILKFVNS CLDSDNSSR 
      FHKLVSNYF KNNSECQNP NQLSSVWCK CNISVTPEN 
      SRSLTWPVR YLHELKDSK TVTSKWIKI FVGCKSEIM 
      KNFVIQGLV TLFLDNPTL GSNVVQFLE HISAAETNP 
      NKDFVTKFM RLVELIVTS PSLSKSDVK KLSSTILCY 
      CHQNLYFYK SDGNDEAID KLCSCVENI VTIHKEYSV 
      LAELATHLK NGHTKNLEK FAKRLIPFL EALMLNDDN 
      SIANNTRKL IGLLELKEN GREPMISAT RSLRIMNMT 
      NASPKLGTK AGNFRTSPL ASKGKGASP KVKKEPVLS 
      INEDKSSDY VPIDTKVSL NVDNLNKHQ KEMLRRRRD 
      DIPALYEDL TRSQTPDMF RSNDETETQ TFDALEEIK 
      EEKSLNSLA SELSSLFDR EKKTEPTPP DTVDKKIES 
      RRKTTPKKL RAEIYNAVQ KQLEQSQTT EVNQNVVRS 
      IEDNFKVVD ESVQNTEDT PVRKSQRVN RRELDFLPT 
      TSSTQNEEE SVKAEVSLE DLLARVDKR VEDSRKNEE 
      VADDNQKKV PVITIQKFV QSSARTRKN PKPMNKPSF 
      KIKLKKRTA LVEKKQQTK TENTESVYD FKADEDTPS 
      DKAKNKVKR GKRKIYLDG SDYSTKKRR RVAKKQVSH 
      VTAETSPDQ NSNTPTPGP TARCPTPVS TSRCPTPTP 
      VTNNRSSRR CPTPLPTNS ALESENSVP TSPSPDSTI 
      KSSVSRRRC PTPVSMISS VPTTCPAPE PKNTSPTPV 
      STSAEPTPV STGKRRRCP TPVQTTSST PEPATVLTN 
      KSSGLRRRP TTPVPDNKN SDPSISPTP ETIITVPTP 
      VSTSKSTGI RKRSTTPVP NSENAIPSS DSTPELEKT 
      TSINTDPTP VLTSKSSVS KRRPTTPVP SSSSLPEPK 
      NTSPTNSNP TPVATSKSS VLRKRPPTP VPPISSAPE 
      PENTSPTNS NPTPVASSK STGLKKRST TPVPNSENA 
      IPSSDSTPE LEKTTSINS DATPALTSK SSVSRRRPS 
      TPVPSSSSL PEPKNTSPA PGSTASNPT PVSTKKRSI 
      LKRRYTTSV PSTSSTPEP KNTSPTNSN PTPVVTSKG 
      PVLRKRPPT PVPQISSAP DPDNTSPTN SNPTPVASS 
      KSTGLRKRS TTPVPNSEN AITSSDSTP ELEKTTSIN 
      SDATPALTS KSSVSRRRP TTPVPSSSS LPEPKNTSP 
      FPGSTSSNP TPVSTKKSS ILKRRFTTS VPTTSLTPE 
      PENTSPTIS NPTPVATSK GSVLRKRPT TPVPNSKNS 
      VSSNSSTPE PKNTSPTPA PTNSSPSPV SNSKRRCTT 
      PVPNDKNSV SPTSLTPDS TSSVPTSSS PSPLGPDDV 
      KTLSVIITP LDQKKESKE NTETGKTQA PLHRRSRKQ 
      SFETLRCSE DVIESSQAT QNNHFTRFQ SRSRQNRTL 
      KQQTIDEIL ATPNKPKQL VTISVILPK ETPKKARQS 
      LPEVITQNT DPVVRLERS QSLDNSEQK TTEMASDTE 
      TLTLESVTT DEKIQDTDT LTLTPVKPP TPDDLPGSP 
      VTCDTPDRT SELLNNTLD ISPISSEQE APPTPAPIP 
      NRRTARLLR MANSSLQIA NSDRKWFGK KPPSPSVCK 
      MKLRKEETL DLLTFSREV PSPMTVPAS GILKRKSED 
      DSGAPVAKR KRVNFSDPA ITERKIFIR DSEEFEDQN 
      QTENNTDEE ESEEKPETS VLEIEELIL NCKEFCDSN 
      FEDSAKGKQ ALKLLMQNL KVPDVVDVI SSPLVDSLV 
      NAVDDRLAT PIKEAQNNA VVKQEITQE QIEEHVVKL 
      DCDNMKNLI KNYLTNLED QERNRFLID LMSLASNEL 
      PVNKEFVNS HAAILQNFV KRMTE
    CDS sequence
    • >TC012353 RA
      atgaacgaa atagtgtcc caagtgcaa agcgggagc 
      tttgatctc attgaatta tttaacgaa agaacaaat 
      gagattgct gagataaag gctgcggtt aagaggcta 
      acaagtact ctacctaga gataagtgc attgatgtt 
      ttcgtaacg aaacttgta ccggctatg tttgccaaa 
      aatgtagat gactggatt agagacgag ctgattgag 
      cttggctct catttcgtc cagtttgtt actgtttcc 
      acgttagat accaaagac caagaggaa gtcttcgct 
      gcgattgac aacacgtac attaaagtt gtcggccag 
      ttgcgagac agtaactac ccgcattgg gaccaactg 
      tgggagttt ttgattaag ttaagcaac gagaagctc 
      cactcgagg caaggcacc ggtaacaaa ctgctgaaa 
      gaggtggag gttgcgttc cgaaactcc tcctcaacc 
      gagcagaga ctgaaagcg tacgactgt tggaaggaa 
      ttgataaac aacagtagc ctaaacatg gagtacatg 
      tgcagcgcc aaacagctc aaattactg gtgaccccc 
      atgaaggcc aaattttcg agaacccca accgttatc 
      ataaagcgt tgcgacacc tacatctat ttgttggag 
      aagttgcaa gacaaggcc actttatgt ctgaaggag 
      tttctagag ttttgcttt ggaccgttt gatgagaaa 
      aatgactcg gaacggacg aggcaaggg aggagcgtc 
      ccggaagtt tgctgcaaa agtaccaaa gttttgctt 
      gagattatt ggccactcg cacgatccc gcggcaaag 
      tgcctgaaa agtgacgag ttccagctg ttcaagcct 
      ttaatcacg aatgagaat tttcccagt tttgccgaa 
      attttaata aattctgtc atggaatgc tgtactttg 
      cttcatcac tgtcagcag aacaagatc atcaatcgt 
      gttattatt cagtgcctt tggcagtca cttcttcgg 
      ctgattcac cagagtagc gacgaagtt aagaagaaa 
      tgttgcgcc caaatcaat agtctatta aacaaagtg 
      ctgaggcac tccggtcag agcgaattc ttatacctg 
      gtgctaggg aacatagtg gagttgcta gtccgtgac 
      aagctcgtg gacgagttc aaagacatt gtgttcgca 
      attgcggtg catctgctc tggatgatc aattttcag 
      acttgcgct tttttggaa ttctacatt ttgaaattc 
      gtcaactcc tgcctggac tcggacaac tccagcaga 
      tttcacaaa ctcgtctcg aattatttc aaaaacaac 
      tccgaatgc caaaacccg aaccaactg agcagtgtt 
      tggtgcaag tgtaatatt tcagtcaca ccggagaat 
      tcccgttcg ttaacgtgg cccgtccgt tatctccac 
      gagctgaaa gactcaaaa accgtcaca tccaagtgg 
      atcaaaata ttcgtcggt tgcaagtcg gaaattatg 
      aaaaatttc gtgatccaa ggacttgtg acgcttttc 
      ttggataat ccgactctt ggctcgaat gtagtgcaa 
      tttttagaa cacatttcg gcggccgaa accaacccc 
      aacaaagat ttcgtgact aaatttatg agacttgtt 
      gagttgatc gtcacttcg cccagcttg tccaagagc 
      gatgtgaaa aagcttagc tcgactatt ctgtgttac 
      tgccaccaa aacttgtac ttttataag tctgatggt 
      aacgatgaa gcaattgac aagttgtgt agttgcgtg 
      gaaaacatt gttacaatt cacaaggaa tattcggtt 
      ctggccgaa ttggccacg catttgaaa aacggccat 
      acgaaaaat ttggaaaag ttcgccaag cgtttgatt 
      ccattccta gaagcgctc atgttaaat gacgacaat 
      tccattgct aataatacg agaaaatta attgggctg 
      ttggaacta aaagaaaac ggacgggaa cctatgata 
      tctgcaaca agatctttg cggataatg aacatgacg 
      aatgcgagc ccaaaactg gggaccaaa gccggcaat 
      tttaggact tcgcccctg gcgtccaaa ggtaaaggg 
      gcttcgccc aaggtcaag aaagaacca gttttgtcc 
      ataaacgaa gacaagtct tccgattat gtcccaatt 
      gacacaaaa gtcagcctc aatgtggac aatctcaac 
      aagcatcag aaagagatg ttgaggaga agacgagat 
      gacatacct gcattgtat gaagacttg actcgaagt 
      caaactcca gacatgttc aggtcgaat gatgaaacc 
      gaaacacag acttttgat gctttggaa gaaattaag 
      gaagaaaag tccttgaat tcgctcgct tctgaatta 
      agcagtctg tttgataga gagaagaag actgaacca 
      acgcctcca gacacagtg gataaaaaa atagaatct 
      cgtcgtaaa acgacaccg aaaaaacta cgggccgag 
      atttacaac gctgtacaa aaacaactg gagcaatcg 
      caaacaacg gaagtaaat caaaacgtc gttcgcagt 
      attgaagat aatttcaag gtggtggac gaaagcgtc 
      caaaacacc gaagataca cccgttcgt aaaagccag 
      cgtgtcaac agacgcgaa cttgatttc ttaccaact 
      acatccagc acacaaaat gaggaagag agcgtgaaa 
      gctgaagta agtttggaa gatctgcta gcacgagtg 
      gacaaaaga gtcgaagat tcgcgaaaa aatgaagaa 
      gtcgccgat gacaatcaa aagaaagtc ccggttata 
      acgattcag aaattcgtt cagagttct gctaggact 
      cggaaaaat cctaaaccg atgaataaa cccagtttt 
      aagatcaag ctgaagaaa cggaccgct ctcgtcgaa 
      aaaaaacaa caaacaaaa accgaaaat actgaaagc 
      gtttacgac tttaaagcc gacgaggac actccgtca 
      gataaggcg aaaaataag gtgaagaga gggaaaagg 
      aagatctac ctagacggt agcgattat agtaccaag 
      aaacgtaga agagtagcg aaaaaacaa gtaagtcac 
      gtcacggcg gaaacatcc ccggaccag aacagtaac 
      actccgact ccgggcccg acagctaga tgcccaact 
      ccggtttcg acaagtaga tgtccaact ccaactccg 
      gttacgaac aataggagt tcgagaaga tgtccgaca 
      ccacttccg acaaacagt gctttagaa agtgaaaat 
      tccgttccg acaagcccc agtccggat tcaaccatt 
      aaaagttcg gtttcgagg agaagatgt ccgactccg 
      gtttcaatg ataagttcc gttccaaca acttgtccg 
      gccccggag ccgaaaaac acgagtccg actccggtt 
      tcgacaagt gctgaaccc actccggtt tcaaccggt 
      aaaagaaga agatgcccg actccggtt cagacaact 
      agttccacc ccggagccg gctactgtt ttgaccaat 
      aaaagttcc ggtctgaga agaagacct acgactccg 
      gttccggat aataaaaat tctgatcca tcaattagt 
      ccgactccg gagacgata attaccgtt ccgactccg 
      gtttcgacc agtaaaagt accggtata agaaagaga 
      tctacgact ccggttccg aatagtgaa aacgcaatt 
      ccgtcaagt gattcgact ccggagctg gaaaaaacc 
      acttcgata aatactgat cccactccg gttttgacc 
      agtaaaagt tccgtttca aaaagaaga cctacgact 
      ccggtgccg tcaagtagt tcgcttccg gagccgaaa 
      aataccagt ccgacaaat tctaatccc actccggtt 
      gcgaccagt aaaagttct gttttaaga aaaaggccc 
      ccgactccg gttccgcca attagttcg gctccggag 
      ccggaaaat accagtccg acaaattct aatcctact 
      ccggttgcg agcagtaaa agtaccggt ttaaaaaag 
      agatctacg actccggtt ccgaatagt gaaaacgca 
      attccgtca agtgattcg actccggag ctggaaaaa 
      accacttcg ataaattct gatgccact ccggcttta 
      accagtaaa agttccgtt tcaagaaga agaccttcg 
      actccggtg ccgtcaagt agttcgctt ccggagccg 
      aaaaatacc agtccggct ccgggttct acagcttct 
      aaccccact ccagtttcg accaaaaaa aggtccatt 
      ttaaaaaga agatatacg acttcggtt ccgtcaact 
      agttcgacc ccggagcca aaaaacacc agtccgaca 
      aattctaat cccactccg gttgtgacc agtaaaggt 
      cccgtttta agaaaaagg cccccgact ccggttccg 
      caaataagt tcggctccg gatccagat aatactagt 
      ccgacaaat tctaatcct actccggtt gcgagcagt 
      aaaagtacc ggtttaaga aagagatct acgactccg 
      gttccgaat agtgaaaac gcaattacg tcaagtgat 
      tcgactccg gagctggaa aaaaccact tcgataaat 
      tctgatgcc actccggct ttgaccagt aaaagttcc 
      gtttcaaga agaagacct acgactccg gtgccgtca 
      agtagttcg cttccggaa ccgaaaaat accagtccg 
      tttccgggt tctacaagt tctaacccc actccagtt 
      tcgaccaaa aaaagttcc attttaaaa agaagattt 
      acgacttcg gttccgaca actagtttg actccggag 
      ccggaaaat accagtccg acaatctct aatcccact 
      ccggttgcg accagtaaa ggttccgtt ttaagaaaa 
      agacctacg actccggtt ccgaatagt aaaaactcc 
      gtttcgtca aatagttcg actccggag ccgaaaaat 
      accagtccg actccagct ccgacaaat tctagtccg 
      agtccggtg tcgaacagt aaaagaagg tgtacgact 
      ccggttccg aatgataaa aattctgtt tcgccaact 
      agtctgacc ccagattcg accagttca gttccgaca 
      agtagtagt ccgtctccg cttgggcca gatgatgtc 
      aaaactctt tcagtgata ataacacca ttggatcaa 
      aagaaagaa tcaaaggaa aatactgaa accggtaaa 
      acccaggcc ccattgcac agacgtagt cgtaagcaa 
      tctttcgaa actctcaga tgttccgaa gacgtgatt 
      gagagttcg caagcgact cagaataac catttcacg 
      agattccag tctcgatct cgccaaaat cgaactttg 
      aaacaacaa acgattgac gaaatcctc gcaactccc 
      aacaaaccc aagcaactg gtcaccata tcggtgata 
      ttaccgaag gaaacgccc aaaaaagct cgccaatcg 
      ctgcctgaa gtcattacg caaaacacc gacccggtc 
      gtgcgcctc gagcgcagc caatcgctg gacaactcc 
      gagcaaaaa accaccgaa atggccagt gacactgaa 
      acactgacc ctagagtcg gttacaacc gacgagaaa 
      atccaagac actgacacg ttgacttta accccggtt 
      aaaccgcca acccctgac gacttgccc ggatcgccg 
      gtcacgtgt gacactccg gaccgcacc agcgaactg 
      ctgaacaac actttggac atctccccc attagttcc 
      gaacaggag gctccgcca acgcccgcc ccgatcccg 
      aacagacgg acggcccgc ttgctacgc atggccaac 
      tcgagcctg cagatcgcg aactcggac cggaaatgg 
      ttcggtaaa aagcctccg tcgcccagc gtgtgcaag 
      atgaagctg cgcaaagag gagactttg gacctcctg 
      acgttttcg cgggaagta ccgagtccg atgacggtc 
      ccagccagc gggattttg aagcgcaag agcgaggac 
      gatagcggg gcccccgtt gcgaagcgc aaacgggtg 
      aatttctcc gacccggcg atcaccgaa aggaagatt 
      tttatccgg gattccgaa gagtttgag gatcagaat 
      cagactgag aataatact gatgaagaa gagtcggaa 
      gagaagccg gaaacgagt gttcttgag attgaagaa 
      ttgatttta aattgtaaa gagttctgt gattctaat 
      ttcgaagat agtgctaaa ggcaagcaa gcacttaag 
      cttcttatg caaaatttg aaggttcct gatgttgtg 
      gacgtgatt tcgtccccg ttggtggac tctctggtt 
      aatgcggtt gatgaccgg ttggctact ccgataaag 
      gaggcgcaa aacaacgca gttgttaaa caggaaata 
      actcaagag caaattgaa gagcacgtt gttaaactt 
      gattgtgat aatatgaaa aatcttatt aagaattat 
      ttgacgaat ttggaggac caggagagg aataggttt 
      ttaattgat ttgatgagt ttagccagc aatgagctt 
      cctgtcaac aaggagttt gtcaattct cacgcggct 
      attttacag aattttgtt aagagaatg actgagtaa
    mRNA sequence
    • >TC012353 RA
      atgacacag aattcccgc gttgacatt gagacgcta 
      agtttgtga tcgatgtgt tttaaataa gttttcggg 
      ctctctatg ttagtaagg ctttaaaat gaacgaaat 
      agtgtccca agtgcaaag cgggagctt tgatctcat 
      tgaattatt taacgaaag aacaaatga gattgctga 
      gataaaggc tgcggttaa gaggctaac aagtactct 
      acctagaga taagtgcat tgatgtttt cgtaacgaa 
      acttgtacc ggctatgtt tgccaaaaa tgtagatga 
      ctggattag agacgagct gattgagct tggctctca 
      tttcgtcca gtttgttac tgtttccac gttagatac 
      caaagacca agaggaagt cttcgctgc gattgacaa 
      cacgtacat taaagttgt cggccagtt gcgagacag 
      taactaccc gcattggga ccaactgtg ggagttttt 
      gattaagtt aagcaacga gaagctcca ctcgaggca 
      aggcaccgg taacaaact gctgaaaga ggtggaggt 
      tgcgttccg aaactcctc ctcaaccga gcagagact 
      gaaagcgta cgactgttg gaaggaatt gataaacaa 
      cagtagcct aaacatgga gtacatgtg cagcgccaa 
      acagctcaa attactggt gacccccat gaaggccaa 
      attttcgag aaccccaac cgttatcat aaagcgttg 
      cgacaccta catctattt gttggagaa gttgcaaga 
      caaggccac tttatgtct gaaggagtt tctagagtt 
      ttgctttgg accgtttga tgagaaaaa tgactcgga 
      acggacgag gcaagggag gagcgtccc ggaagtttg 
      ctgcaaaag taccaaagt tttgcttga gattattgg 
      ccactcgca cgatcccgc ggcaaagtg cctgaaaag 
      tgacgagtt ccagctgtt caagccttt aatcacgaa 
      tgagaattt tcccagttt tgccgaaat tttaataaa 
      ttctgtcat ggaatgctg tactttgct tcatcactg 
      tcagcagaa caagatcat caatcgtgt tattattca 
      gtgcctttg gcagtcact tcttcggct gattcacca 
      gagtagcga cgaagttaa gaagaaatg ttgcgccca 
      aatcaatag tctattaaa caaagtgct gaggcactc 
      cggtcagag cgaattctt atacctggt gctagggaa 
      catagtgga gttgctagt ccgtgacaa gctcgtgga 
      cgagttcaa agacattgt gttcgcaat tgcggtgca 
      tctgctctg gatgatcaa ttttcagac ttgcgcttt 
      tttggaatt ctacatttt gaaattcgt caactcctg 
      cctggactc ggacaactc cagcagatt tcacaaact 
      cgtctcgaa ttatttcaa aaacaactc cgaatgcca 
      aaacccgaa ccaactgag cagtgtttg gtgcaagtg 
      taatatttc agtcacacc ggagaattc ccgttcgtt 
      aacgtggcc cgtccgtta tctccacga gctgaaaga 
      ctcaaaaac cgtcacatc caagtggat caaaatatt 
      cgtcggttg caagtcgga aattatgaa aaatttcgt 
      gatccaagg acttgtgac gcttttctt ggataatcc 
      gactcttgg ctcgaatgt agtgcaatt tttagaaca 
      catttcggc ggccgaaac caaccccaa caaagattt 
      cgtgactaa atttatgag acttgttga gttgatcgt 
      cacttcgcc cagcttgtc caagagcga tgtgaaaaa 
      gcttagctc gactattct gtgttactg ccaccaaaa 
      cttgtactt ttataagtc tgatggtaa cgatgaagc 
      aattgacaa gttgtgtag ttgcgtgga aaacattgt 
      tacaattca caaggaata ttcggttct ggccgaatt 
      ggccacgca tttgaaaaa cggccatac gaaaaattt 
      ggaaaagtt cgccaagcg tttgattcc attcctaga 
      agcgctcat gttaaatga cgacaattc cattgctaa 
      taatacgag aaaattaat tgggctgtt ggaactaaa 
      agaaaacgg acgggaacc tatgatatc tgcaacaag 
      atctttgcg gataatgaa catgacgaa tgcgagccc 
      aaaactggg gaccaaagc cggcaattt taggacttc 
      gcccctggc gtccaaagg taaaggggc ttcgcccaa 
      ggtcaagaa agaaccagt tttgtccat aaacgaaga 
      caagtcttc cgattatgt cccaattga cacaaaagt 
      cagcctcaa tgtggacaa tctcaacaa gcatcagaa 
      agagatgtt gaggagaag acgagatga catacctgc 
      attgtatga agacttgac tcgaagtca aactccaga 
      catgttcag gtcgaatga tgaaaccga aacacagac 
      ttttgatgc tttggaaga aattaagga agaaaagtc 
      cttgaattc gctcgcttc tgaattaag cagtctgtt 
      tgatagaga gaagaagac tgaaccaac gcctccaga 
      cacagtgga taaaaaaat agaatctcg tcgtaaaac 
      gacaccgaa aaaactacg ggccgagat ttacaacgc 
      tgtacaaaa acaactgga gcaatcgca aacaacgga 
      agtaaatca aaacgtcgt tcgcagtat tgaagataa 
      tttcaaggt ggtggacga aagcgtcca aaacaccga 
      agatacacc cgttcgtaa aagccagcg tgtcaacag 
      acgcgaact tgatttctt accaactac atccagcac 
      acaaaatga ggaagagag cgtgaaagc tgaagtaag 
      tttggaaga tctgctagc acgagtgga caaaagagt 
      cgaagattc gcgaaaaaa tgaagaagt cgccgatga 
      caatcaaaa gaaagtccc ggttataac gattcagaa 
      attcgttca gagttctgc taggactcg gaaaaatcc 
      taaaccgat gaataaacc cagttttaa gatcaagct 
      gaagaaacg gaccgctct cgtcgaaaa aaaacaaca 
      aacaaaaac cgaaaatac tgaaagcgt ttacgactt 
      taaagccga cgaggacac tccgtcaga taaggcgaa 
      aaataaggt gaagagagg gaaaaggaa gatctacct 
      agacggtag cgattatag taccaagaa acgtagaag 
      agtagcgaa aaaacaagt aagtcacgt cacggcgga 
      aacatcccc ggaccagaa cagtaacac tccgactcc 
      gggcccgac agctagatg cccaactcc ggtttcgac 
      aagtagatg tccaactcc aactccggt tacgaacaa 
      taggagttc gagaagatg tccgacacc acttccgac 
      aaacagtgc tttagaaag tgaaaattc cgttccgac 
      aagccccag tccggattc aaccattaa aagttcggt 
      ttcgaggag aagatgtcc gactccggt ttcaatgat 
      aagttccgt tccaacaac ttgtccggc cccggagcc 
      gaaaaacac gagtccgac tccggtttc gacaagtgc 
      tgaacccac tccggtttc aaccggtaa aagaagaag 
      atgcccgac tccggttca gacaactag ttccacccc 
      ggagccggc tactgtttt gaccaataa aagttccgg 
      tctgagaag aagacctac gactccggt tccggataa 
      taaaaattc tgatccatc aattagtcc gactccgga 
      gacgataat taccgttcc gactccggt ttcgaccag 
      taaaagtac cggtataag aaagagatc tacgactcc 
      ggttccgaa tagtgaaaa cgcaattcc gtcaagtga 
      ttcgactcc ggagctgga aaaaaccac ttcgataaa 
      tactgatcc cactccggt tttgaccag taaaagttc 
      cgtttcaaa aagaagacc tacgactcc ggtgccgtc 
      aagtagttc gcttccgga gccgaaaaa taccagtcc 
      gacaaattc taatcccac tccggttgc gaccagtaa 
      aagttctgt tttaagaaa aaggccccc gactccggt 
      tccgccaat tagttcggc tccggagcc ggaaaatac 
      cagtccgac aaattctaa tcctactcc ggttgcgag 
      cagtaaaag taccggttt aaaaaagag atctacgac 
      tccggttcc gaatagtga aaacgcaat tccgtcaag 
      tgattcgac tccggagct ggaaaaaac cacttcgat 
      aaattctga tgccactcc ggctttaac cagtaaaag 
      ttccgtttc aagaagaag accttcgac tccggtgcc 
      gtcaagtag ttcgcttcc ggagccgaa aaataccag 
      tccggctcc gggttctac agcttctaa ccccactcc 
      agtttcgac caaaaaaag gtccatttt aaaaagaag 
      atatacgac ttcggttcc gtcaactag ttcgacccc 
      ggagccaaa aaacaccag tccgacaaa ttctaatcc 
      cactccggt tgtgaccag taaaggtcc cgttttaag 
      aaaaaggcc cccgactcc ggttccgca aataagttc 
      ggctccgga tccagataa tactagtcc gacaaattc 
      taatcctac tccggttgc gagcagtaa aagtaccgg 
      tttaagaaa gagatctac gactccggt tccgaatag 
      tgaaaacgc aattacgtc aagtgattc gactccgga 
      gctggaaaa aaccacttc gataaattc tgatgccac 
      tccggcttt gaccagtaa aagttccgt ttcaagaag 
      aagacctac gactccggt gccgtcaag tagttcgct 
      tccggaacc gaaaaatac cagtccgtt tccgggttc 
      tacaagttc taaccccac tccagtttc gaccaaaaa 
      aagttccat tttaaaaag aagatttac gacttcggt 
      tccgacaac tagtttgac tccggagcc ggaaaatac 
      cagtccgac aatctctaa tcccactcc ggttgcgac 
      cagtaaagg ttccgtttt aagaaaaag acctacgac 
      tccggttcc gaatagtaa aaactccgt ttcgtcaaa 
      tagttcgac tccggagcc gaaaaatac cagtccgac 
      tccagctcc gacaaattc tagtccgag tccggtgtc 
      gaacagtaa aagaaggtg tacgactcc ggttccgaa 
      tgataaaaa ttctgtttc gccaactag tctgacccc 
      agattcgac cagttcagt tccgacaag tagtagtcc 
      gtctccgct tgggccaga tgatgtcaa aactctttc 
      agtgataat aacaccatt ggatcaaaa gaaagaatc 
      aaaggaaaa tactgaaac cggtaaaac ccaggcccc 
      attgcacag acgtagtcg taagcaatc tttcgaaac 
      tctcagatg ttccgaaga cgtgattga gagttcgca 
      agcgactca gaataacca tttcacgag attccagtc 
      tcgatctcg ccaaaatcg aactttgaa acaacaaac 
      gattgacga aatcctcgc aactcccaa caaacccaa 
      gcaactggt caccatatc ggtgatatt accgaagga 
      aacgcccaa aaaagctcg ccaatcgct gcctgaagt 
      cattacgca aaacaccga cccggtcgt gcgcctcga 
      gcgcagcca atcgctgga caactccga gcaaaaaac 
      caccgaaat ggccagtga cactgaaac actgaccct 
      agagtcggt tacaaccga cgagaaaat ccaagacac 
      tgacacgtt gactttaac cccggttaa accgccaac 
      ccctgacga cttgcccgg atcgccggt cacgtgtga 
      cactccgga ccgcaccag cgaactgct gaacaacac 
      tttggacat ctcccccat tagttccga acaggaggc 
      tccgccaac gcccgcccc gatcccgaa cagacggac 
      ggcccgctt gctacgcat ggccaactc gagcctgca 
      gatcgcgaa ctcggaccg gaaatggtt cggtaaaaa 
      gcctccgtc gcccagcgt gtgcaagat gaagctgcg 
      caaagagga gactttgga cctcctgac gttttcgcg 
      ggaagtacc gagtccgat gacggtccc agccagcgg 
      gattttgaa gcgcaagag cgaggacga tagcggggc 
      ccccgttgc gaagcgcaa acgggtgaa tttctccga 
      cccggcgat caccgaaag gaagatttt tatccggga 
      ttccgaaga gtttgagga tcagaatca gactgagaa 
      taatactga tgaagaaga gtcggaaga gaagccgga 
      aacgagtgt tcttgagat tgaagaatt gattttaaa 
      ttgtaaaga gttctgtga ttctaattt cgaagatag 
      tgctaaagg caagcaagc acttaagct tcttatgca 
      aaatttgaa ggttcctga tgttgtgga cgtgatttc 
      gtccccgtt ggtggactc tctggttaa tgcggttga 
      tgaccggtt ggctactcc gataaagga ggcgcaaaa 
      caacgcagt tgttaaaca ggaaataac tcaagagca 
      aattgaaga gcacgttgt taaacttga ttgtgataa 
      tatgaaaaa tcttattaa gaattattt gacgaattt 
      ggaggacca ggagaggaa taggttttt aattgattt 
      gatgagttt agccagcaa tgagcttcc tgtcaacaa 
      ggagtttgt caattctca cgcggctat tttacagaa 
      ttttgttaa gagaatgac tgagtaatt gttagttat 
      tagtattat cacagttta cgttgtatt atcaatatc 
      tgtacctta ttaaaattt tgtatatct a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY