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Gene information
General information
TC012351 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5399345..5428756
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012351
      iBeetle screen iB_05222

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012351 RA
        MSYGYVPQY VTEEPESES ECPSDDQIM QHVTSDVTE 
        LYRRKHQTV NHHRNSAGT YYHTHVPNC YEQQTRIPA 
        TCACNLPPL DPRYCYYKA VPQAQEGIF RSSTTVSPN 
        LRTCQDTKA ASTATFKKY PVTARTPRL GRYSGEGVR 
        PRAVKVVLN NEDEVKAVV FNAKELKNH ANYRNISLS 
        MDKTPRQIA YYKQLQQEL NDRTSKGET APTPTEIQH 
        YRGQEAAYA INDYVMRTT AVKRTDTRP VDSEGDVHS 
        RLNDLENFL TRFDDQAED DGDVEIAGV SEISKILKP 
        KVPASVKAK HQDKIIKLR AVKTNVTET ITTPEKVSK 
        LEVTEAPEE PLNVLLQKL NKDSPKPKE AKSENEVKR 
        ESKFKTVMG KNRQFCIEQ KPPLDDNSA LELHKSKSY 
        IVSLIDKAL SRELGTVPG DKCTRKEFM TMNPKKAID 
        LVNRHRSAS QGAEKSINL DVTQASSSK KDDQCVCKN 
        EEPLYLKQL KQLRWSHLR HIQREAKRL ADLERFLDS 
        FSEGESPP
      CDS sequence
      • >TC012351 RA
        atgtcttac ggttacgtc ccccagtac gtaacggag 
        gagccggag tccgagagc gagtgtccc tctgacgac 
        caaatcatg caacacgtt acatccgac gttaccgaa 
        ctgtaccgg aggaaacat caaaccgta aaccatcac 
        cggaactct gccggaaca tattatcac acgcacgtg 
        cccaactgt tacgaacaa caaacccga atcccggcg 
        acttgtgcg tgcaacctg ccgccgctt gatcccaga 
        tactgctat tataaagcg gttcctcaa gcccaggaa 
        gggatattc agaagttct acaactgtc tcaccgaat 
        ttgcggact tgtcaggac acgaaagcc gcctcaacg 
        gccacgttc aagaaatac ccggtcacg gcaaggaca 
        ccaagatta ggacgttat tctggagaa ggtgtgcgt 
        ccacgagca gttaaagtg gttcttaat aatgaggat 
        gaagttaaa gccgtagtt ttcaatgct aaagaactg 
        aaaaatcac gctaattat cgcaatatt tcactatct 
        atggacaaa acacctaga caaattgct tactacaaa 
        caacttcaa caggaatta aacgaccgc actagcaaa 
        ggcgaaacg gctccaacg cccacagaa attcagcat 
        tatcgcggt caagaagct gcctacgca attaacgac 
        tacgtcatg agaacgacg gcggtcaag agaacagac 
        acccgtcct gtcgattcc gaaggagac gttcattcc 
        agattgaac gacctggaa aatttcctc accagattc 
        gacgaccag gccgaagac gacggcgac gtcgagatc 
        gccggcgtc agcgaaata tcgaagatt ttgaagccg 
        aaggtcccg gcatcggtc aaggccaaa caccaggac 
        aaaattatc aaactaagg gctgttaaa acgaacgta 
        actgaaaca atcacaacc cccgagaaa gtaagtaaa 
        ttagaagta acggaagcg cccgaagaa cccctaaac 
        gtactgtta caaaaactg aataaagac agtcctaag 
        ccgaaagaa gcgaaaagc gagaacgaa gtgaaacgc 
        gagtcgaag tttaaaacg gtaatgggg aaaaatcgg 
        cagttttgc atcgaacag aaaccgcca ctggacgat 
        aactcggcc ctggagttg cacaaatcg aagagttac 
        atcgtgagt ctgattgac aaagctttg agtcgggag 
        ctgggcact gttcctggc gacaagtgt acgagaaaa 
        gagtttatg acaatgaat ccgaagaag gcgatcgat 
        ttggtcaat cgacacagg tccgcgagt caaggtgca 
        gaaaaatcg atcaatctc gacgtcaca caggcgagt 
        tcttccaag aaggacgat cagtgtgtt tgcaaaaac 
        gaggagccg ttgtacttg aagcaactg aaacaactc 
        cgctggagt cacctgaga cacattcag cgcgaagcc 
        aaacgtctc gccgacctg gaacgcttc ctcgactcg 
        ttcagcgaa ggggagagt ccgccgtaa
      mRNA sequence
      • >TC012351 RA
        aggattaat ataatcacg tcggtgggt gtcacgtct 
        ctacttgtc attattttt gttgtcgca cattttttt 
        ctctcttga atgttaata aatgaagca gtgcccgtc 
        aatatccag ctccagctc ttgagtggt ctcattaag 
        gtccaagat gtcttacgg ttacgtccc ccagtacgt 
        aacggagga gccggagtc cgagagcga gtgtccctc 
        tgacgacca aatcatgca acacgttac atccgacgt 
        taccgaact gtaccggag gaaacatca aaccgtaaa 
        ccatcaccg gaactctgc cggaacata ttatcacac 
        gcacgtgcc caactgtta cgaacaaca aacccgaat 
        cccggcgac ttgtgcgtg caacctgcc gccgcttga 
        tcccagata ctgctatta taaagcggt tcctcaagc 
        ccaggaagg gatattcag aagttctac aactgtctc 
        accgaattt gcggacttg tcaggacac gaaagccgc 
        ctcaacggc cacgttcaa gaaataccc ggtcacggc 
        aaggacacc aagattagg acgttattc tggagaagg 
        tgtgcgtcc acgagcagt taaagtggt tcttaataa 
        tgaggatga agttaaagc cgtagtttt caatgctaa 
        agaactgaa aaatcacgc taattatcg caatatttc 
        actatctat ggacaaaac acctagaca aattgctta 
        ctacaaaca acttcaaca ggaattaaa cgaccgcac 
        tagcaaagg cgaaacggc tccaacgcc cacagaaat 
        tcagcatta tcgcggtca agaagctgc ctacgcaat 
        taacgacta cgtcatgag aacgacggc ggtcaagag 
        aacagacac ccgtcctgt cgattccga aggagacgt 
        tcattccag attgaacga cctggaaaa tttcctcac 
        cagattcga cgaccaggc cgaagacga cggcgacgt 
        cgagatcgc cggcgtcag cgaaatatc gaagatttt 
        gaagccgaa ggtcccggc atcggtcaa ggccaaaca 
        ccaggacaa aattatcaa actaagggc tgttaaaac 
        gaacgtaac tgaaacaat cacaacccc cgagaaagt 
        aagtaaatt agaagtaac ggaagcgcc cgaagaacc 
        cctaaacgt actgttaca aaaactgaa taaagacag 
        tcctaagcc gaaagaagc gaaaagcga gaacgaagt 
        gaaacgcga gtcgaagtt taaaacggt aatggggaa 
        aaatcggca gttttgcat cgaacagaa accgccact 
        ggacgataa ctcggccct ggagttgca caaatcgaa 
        gagttacat cgtgagtct gattgacaa agctttgag 
        tcgggagct gggcactgt tcctggcga caagtgtac 
        gagaaaaga gtttatgac aatgaatcc gaagaaggc 
        gatcgattt ggtcaatcg acacaggtc cgcgagtca 
        aggtgcaga aaaatcgat caatctcga cgtcacaca 
        ggcgagttc ttccaagaa ggacgatca gtgtgtttg 
        caaaaacga ggagccgtt gtacttgaa gcaactgaa 
        acaactccg ctggagtca cctgagaca cattcagcg 
        cgaagccaa acgtctcgc cgacctgga acgcttcct 
        cgactcgtt cagcgaagg ggagagtcc gccgtaaaa 
        gtttgatat aatttttat ttacaaaat ttatacaat 
        aaacagcag tcgtgtaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY