Status
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Gene information
General information
TC012348 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5267630..5279183
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012348
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05221

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012348 RA
      MLQNGRRLG ALCTAVQNL SLNRNLLAR HNLKLSQRR 
      FKKNDSSIS SLFIPVPVK PNPDDINVG AELTGNLNK 
      ADLLKVLNK FYQKKEIKQ LLTENGLDN YLQHQAYVS 
      FRRYCLEAQ TLPVDLHVV VSDVLQGAG NITDIFPYF 
      LRHAKQMFP HLECMDDLR KISDLRTPA NWYPEARAL 
      NRKIIFHAG PTNSGKTFH ALESFITAK SGVYCGPLK 
      LLAAEVYKK SNSRGTPCD LVTGEERKY ADSSGQPST 
      HVSCTVEMA SLTAPYEVA VIDEIQMVR DHQRGWAWT 
      RAFLGLVAE EIHLCGEAG AIDLISQLC LTTNEDIEV 
      RHYKRLTDL KVEDTALGS LDNVMPGDC IVCFSKNDI 
      YSVSRGIEA TGKEVAVIY GGLPPGTKL AQAAKFNDP 
      ENSCKILVA TDAIGMGLN LSIRRVIFY SLIKPTMNE 
      KGEKEMDTI SVSAALQIA GRAGRYGTQ WEQGFVTTF 
      KPEDLKTLK TLLESLPEP ITQAGLHPT AEQIELYAY 
      HLPNSTLSN LMDIFVSLS TVDDSLYFM CNVEDFKFL 
      ADMIQHVPL PLRARYVFC CAPISKKMP FVCTMFLKF 
      TRQYSKNEP ITFDWLCRN IGWPLQPPK TIIDLVHLE 
      AVFDVLDLY LWLSYRFMD LFNEPDLVR DMQKELDVI 
      IQQGVVQLT RLLRNSETG ARGADEEQF ALNRQKQSY 
      LRESKALLG RGRLTERLL AQGILTPHM LHELKKEWK 
      GGDEEGDGD DEPKKPTRR KRKK
    CDS sequence
    • >TC012348 RA
      atgttgcaa aacgggcga cgcctcgga gccctctgt 
      accgcggtc caaaattta tcgttaaat cggaacctt 
      ttggcccga cataattta aaattatcg caacgacgg 
      tttaaaaaa aacgatagt tccatatca tccctgttc 
      attcctgtg cctgtgaaa ccaaaccct gacgatata 
      aatgtgggg gccgaactg actggcaat ttaaacaaa 
      gccgatttg ctcaaggtc ctcaataag ttctaccaa 
      aagaaggaa atcaagcaa ttacttacg gaaaatggg 
      cttgataac tatctgcaa caccaagcg tacgttagc 
      ttccggcgg tactgtctc gaagcgcag actttacct 
      gtcgatttg cacgttgtt gtgagcgat gttttacaa 
      ggagctggg aacattacc gatattttc ccgtatttt 
      ttacgccac gccaagcaa atgttccca catttggaa 
      tgcatggac gatttaaga aaaatctcg gatctgcga 
      accccagct aactggtac cccgaggcc agggctttg 
      aaccgcaaa attattttt catgcgggc cccactaac 
      tcgggcaag acatttcac gcgttagag agtttcatt 
      actgccaaa tcgggggtt tactgtggc ccccttaaa 
      ctactagcc gccgaagtg tacaaaaaa agtaattca 
      aggggcact ccgtgtgat ttggtaacg ggcgaagaa 
      cggaaatac gctgactcg tccggacag ccctcgact 
      catgtttct tgcactgtg gaaatggct tcgctaact 
      gcaccttat gaggttgcg gttattgat gaaattcag 
      atggtgagg gatcatcag agggggtgg gcgtggact 
      agggccttt ttggggctg gtcgctgag gagatacac 
      ctgtgtggg gaggctggt gccattgat ttgataagc 
      cagttgtgt ttgacgaca aatgaggat attgaagtg 
      aggcattat aaaagactg actgattta aaagtagaa 
      gacacagct ctaggcagt ttggacaac gtgatgccg 
      ggcgactgc atcgtctgc ttcagcaaa aacgacatt 
      tactcagtc tcacgcggc atcgaagcg accggtaaa 
      gaagtcgcc gtgatatac gggggcctc cctcccggc 
      accaagctg gcccaagcc gccaaattc aacgacccg 
      gaaaacagt tgcaaaatt ttggtcgcc accgacgcc 
      atcggaatg ggcctcaac ttgagcatc cgccgcgtt 
      atcttctac tcactcatc aaacccacg atgaacgaa 
      aagggcgag aaggaaatg gacacgatt tcagtctca 
      gccgccctg caaatcgcc ggaagggct ggcagatac 
      ggaacgcaa tgggagcaa ggtttcgtc acaaccttc 
      aagcctgaa gacttgaaa acattgaag acgttgctg 
      gagtcgctc cccgagccc ataacccag gcggggctc 
      caccccacg gccgagcaa atcgagctc tacgcctac 
      cacttgccc aactcaacc cttagcaat ttgatggac 
      attttcgtt agtttatca acggttgat gattcccta 
      tattttatg tgtaacgtg gaggacttt aaatttttg 
      gccgatatg atccagcac gtccccttg ccactgcgg 
      gcccgttat gtcttttgt tgcgccccc attagcaag 
      aaaatgccg tttgtgtgc acaatgttt ctgaaattc 
      actcggcag tacagcaag aatgaaccg attactttc 
      gactggctt tgtcgtaat attgggtgg cccttgcag 
      cccccgaag acaatcatt gatctcgtg catctggag 
      gctgtgttc gatgtgctg gatttgtat ctgtggttg 
      agttaccgc ttcatggat ttgtttaac gagcctgat 
      ttggtgcga gatatgcag aaggagttg gacgtgatt 
      attcaacag ggggtggtc cagttgacg aggttgctg 
      cggaattcc gagacgggg gcccggggc gccgatgag 
      gagcagttt gcgttgaat aggcaaaag cagtcgtat 
      ttgagagag tcgaaggcg ctgcttggg aggggtagg 
      ttgactgag aggctgctg gcgcagggg attttgacg 
      ccgcacatg ttgcacgag ctgaagaag gagtggaag 
      ggcggggat gaagagggc gatggtgat gacgagccg 
      aagaagccg acgaggagg aaacggaag aagtag
    mRNA sequence
    • >TC012348 RA
      acagttatc taaccttaa attttttca gttaacctt 
      gcttttata cgcaacatg ttgcaaaac gggcgacgc 
      ctcggagcc ctctgtacc gcggtccaa aatttatcg 
      ttaaatcgg aaccttttg gcccgacat aatttaaaa 
      ttatcgcaa cgacggttt aaaaaaaac gatagttcc 
      atatcatcc ctgttcatt cctgtgcct gtgaaacca 
      aaccctgac gatataaat gtgggggcc gaactgact 
      ggcaattta aacaaagcc gatttgctc aaggtcctc 
      aataagttc taccaaaag aaggaaatc aagcaatta 
      cttacggaa aatgggctt gataactat ctgcaacac 
      caagcgtac gttagcttc cggcggtac tgtctcgaa 
      gcgcagact ttacctgtc gatttgcac gttgttgtg 
      agcgatgtt ttacaagga gctgggaac attaccgat 
      attttcccg tatttttta cgccacgcc aagcaaatg 
      ttcccacat ttggaatgc atggacgat ttaagaaaa 
      atctcggat ctgcgaacc ccagctaac tggtacccc 
      gaggccagg gctttgaac cgcaaaatt atttttcat 
      gcgggcccc actaactcg ggcaagaca tttcacgcg 
      ttagagagt ttcattact gccaaatcg ggggtttac 
      tgtggcccc cttaaacta ctagccgcc gaagtgtac 
      aaaaaaagt aattcaagg ggcactccg tgtgatttg 
      gtaacgggc gaagaacgg aaatacgct gactcgtcc 
      ggacagccc tcgactcat gtttcttgc actgtggaa 
      atggcttcg ctaactgca ccttatgag gttgcggtt 
      attgatgaa attcagatg gtgagggat catcagagg 
      gggtgggcg tggactagg gcctttttg gggctggtc 
      gctgaggag atacacctg tgtggggag gctggtgcc 
      attgatttg ataagccag ttgtgtttg acgacaaat 
      gaggatatt gaagtgagg cattataaa agactgact 
      gatttaaaa gtagaagac acagctcta ggcagtttg 
      gacaacgtg atgccgggc gactgcatc gtctgcttc 
      agcaaaaac gacatttac tcagtctca cgcggcatc 
      gaagcgacc ggtaaagaa gtcgccgtg atatacggg 
      ggcctccct cccggcacc aagctggcc caagccgcc 
      aaattcaac gacccggaa aacagttgc aaaattttg 
      gtcgccacc gacgccatc ggaatgggc ctcaacttg 
      agcatccgc cgcgttatc ttctactca ctcatcaaa 
      cccacgatg aacgaaaag ggcgagaag gaaatggac 
      acgatttca gtctcagcc gccctgcaa atcgccgga 
      agggctggc agatacgga acgcaatgg gagcaaggt 
      ttcgtcaca accttcaag cctgaagac ttgaaaaca 
      ttgaagacg ttgctggag tcgctcccc gagcccata 
      acccaggcg gggctccac cccacggcc gagcaaatc 
      gagctctac gcctaccac ttgcccaac tcaaccctt 
      agcaatttg atggacatt ttcgttagt ttatcaacg 
      gttgatgat tccctatat tttatgtgt aacgtggag 
      gactttaaa tttttggcc gatatgatc cagcacgtc 
      cccttgcca ctgcgggcc cgttatgtc ttttgttgc 
      gcccccatt agcaagaaa atgccgttt gtgtgcaca 
      atgtttctg aaattcact cggcagtac agcaagaat 
      gaaccgatt actttcgac tggctttgt cgtaatatt 
      gggtggccc ttgcagccc ccgaagaca atcattgat 
      ctcgtgcat ctggaggct gtgttcgat gtgctggat 
      ttgtatctg tggttgagt taccgcttc atggatttg 
      tttaacgag cctgatttg gtgcgagat atgcagaag 
      gagttggac gtgattatt caacagggg gtggtccag 
      ttgacgagg ttgctgcgg aattccgag acgggggcc 
      cggggcgcc gatgaggag cagtttgcg ttgaatagg 
      caaaagcag tcgtatttg agagagtcg aaggcgctg 
      cttgggagg ggtaggttg actgagagg ctgctggcg 
      caggggatt ttgacgccg cacatgttg cacgagctg 
      aagaaggag tggaagggc ggggatgaa gagggcgat 
      ggtgatgac gagccgaag aagccgacg aggaggaaa 
      cggaagaag tagggtccg aaatataca aaatctatt 
      tttacaaac atctattta tgctagaga gaaaattgt 
      acattgatt tgtgcttaa ttacgttcg gaacattga 
      atttgaacg ggttgttaa gccactgtt acaataaaa 
      tataaaaat agatacaaa aacatggtt tattttttc 
      agttattta cagggctaa gcgatgggt ctacaaagg 
      taaaaaact caatatcga ctatgtaca aaaatatta 
      atatcttag gtttacatt caaatttgt actaaatct 
      tcaatcata ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY