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Gene information
General information
TC012346 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5222518..5227750
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012346
    iBeetle screen iB_05219

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012346 RA
      MEGPMVSVY NNDFYPIEK TKAQLANKE LWDQFHDLQ 
      TEMIITKSG RRMFPALNL KITDLDPSA DYCLFLEMT 
      LAHHCRFKF SSSVGWSPA GHEEAQSPH RIYMHPESP 
      SKGSHWMNQ DISFSRVKL TNTCSPPQG QLVLSSMHK 
      YQPRILIVK ASTAQALGW APTNVFTFP ETQFIAVTA 
      YQNEKITKL KINYNPFAK GFRENGKSS AKRKQLVSE 
      HPDSPNSKK SATPSPPPQ SEVNERKEN LTCQPAVPY 
      VPFYRYCYR PYPVYNQWW SGPLPMYYQ YPFGLPEYE 
      YSFYQEVKK PKKITDFSI SAIMKDCK
    CDS sequence
    • >TC012346 RA
      atggaaggc ccaatggtc agtgtgtac aacaatgat 
      ttttacccg attgagaag accaaggcc cagttggcc 
      aataaggaa ctgtgggac cagtttcac gacctgcag 
      acggagatg ataatcacc aaaagtggg aggcgcatg 
      ttcccggcc ttaaacctc aaaatcacc gacttggac 
      ccatccgcc gactactgc ctcttcctg gagatgacc 
      ttagcccac cactgccgc ttcaaattc tcctcttcg 
      gtgggctgg tcccccgca ggccacgag gaggcccag 
      agcccccac cggatctac atgcacccg gagtcgccc 
      tctaaaggc tcgcactgg atgaaccaa gacatctcg 
      ttctcgcga gtcaaactc acaaacaca tgttcgccc 
      cctcaaggc cagttggtt ttatcatcc atgcacaaa 
      taccaaccc cgcatttta atcgtcaag gcttccaca 
      gcccaggct ttaggctgg gcccccacg aacgttttc 
      acctttcct gaaacacag ttcattgca gttacggcc 
      tatcagaat gagaaaatt acaaaacta aaaatcaac 
      tacaaccct tttgcgaag ggttttcgc gaaaatggg 
      aaatcgagt gcgaagcgg aaacagtta gtttccgaa 
      catcccgac tctcccaat agtaagaaa agtgcaact 
      ccttcgcca cccccacaa tcggaagtt aatgagcgc 
      aaggagaat ctgacgtgt cagcctgcg gtgccctac 
      gtgcccttt tataggtat tgttaccgc ccttatcct 
      gtttataat cagtggtgg agtggccct ttacctatg 
      tattaccaa tatccgttt gggcttcct gagtacgaa 
      tactcgttt taccaagaa gtcaaaaag ccgaaaaaa 
      atcaccgat ttctcgatt agtgccatt atgaaagat 
      tgtaagtag
    mRNA sequence
    • >TC012346 RA
      ttgatacga ttttgaaag cgaaaacat aaataaata 
      cgaaattaa attgataac ccactagtg atgacccag 
      agtgccaac caaatccat taataacca aaaaccgtc 
      tttttccac taggaaaaa ggggtaagt tgcaaacaa 
      gattactca ggtagaaaa attaatctg taataattc 
      accccagtg ggggttgtg atgataaca actttgaag 
      aactagtgg taccaccac cacgtgctc atcccctct 
      caagtcaaa aactaaact tttaaatgc gtatttaag 
      gggtgggtg acagtaatt tgctcattt cggacgttg 
      gtgctgtgt gaattttga gtgcgaaat tatggaagg 
      cccaatggt cagtgtgta caacaatga tttttaccc 
      gattgagaa gaccaaggc ccagttggc caataagga 
      actgtggga ccagtttca cgacctgca gacggagat 
      gataatcac caaaagtgg gaggcgcat gttcccggc 
      cttaaacct caaaatcac cgacttgga cccatccgc 
      cgactactg cctcttcct ggagatgac cttagccca 
      ccactgccg cttcaaatt ctcctcttc ggtgggctg 
      gtcccccgc aggccacga ggaggccca gagccccca 
      ccggatcta catgcaccc ggagtcgcc ctctaaagg 
      ctcgcactg gatgaacca agacatctc gttctcgcg 
      agtcaaact cacaaacac atgttcgcc ccctcaagg 
      ccagttggt tttatcatc catgcacaa ataccaacc 
      ccgcatttt aatcgtcaa ggcttccac agcccaggc 
      tttaggctg ggcccccac gaacgtttt cacctttcc 
      tgaaacaca gttcattgc agttacggc ctatcagaa 
      tgagaaaat tacaaaact aaaaatcaa ctacaaccc 
      ttttgcgaa gggttttcg cgaaaatgg gaaatcgag 
      tgcgaagcg gaaacagtt agtttccga acatcccga 
      ctctcccaa tagtaagaa aagtgcaac tccttcgcc 
      acccccaca atcggaagt taatgagcg caaggagaa 
      tctgacgtg tcagcctgc ggtgcccta cgtgccctt 
      ttataggta ttgttaccg cccttatcc tgtttataa 
      tcagtggtg gagtggccc tttacctat gtattacca 
      atatccgtt tgggcttcc tgagtacga atactcgtt 
      ttaccaaga agtcaaaaa gccgaaaaa aatcaccga 
      tttctcgat tagtgccat tatgaaaga ttgtaagta 
      gttctaggt tgcgtattt accaagtct gtgatatta 
      gattgtaat aaatatgaa aatttatct aaacgtttt 
      tccgttcag ttcgtgtgt ttactgatt tctagagct 
      tttattttt atatttaag tgcaataaa taattattc 
      gcaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY