| General information | |||
| TC012341 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 5074786..5084285 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC012341 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05218 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
ovary without detectable defects - Number of animals showing the phenotype: 2
larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC012341 RA
MPGPPPPPP PGMMPPPPP APTSFPKAG KPDDRAALL KSIQKGTRL KKTVTNDRS APVIGGKTS NSNSSSPSG GTPASTPNG LNLGGLFAG GMPKLKPTG KLPANNVNN GKVGLPVKN SSSLQNELK KQMASDNKN RGPPPPAPV RNTPEENGT RQLSALKVG STNGLHLNQ SSLHSNLQQ QNSTLHRKV KSNANLSTL DSPDSINGF NQTSKPVIS HGKPNLAPK PPVLNAKPV APPKKLVIN GKPVGRAHS MRSPRTPSP QSPDANVAM KFGTVRNLS SVIGQSLAN STGNLSPRQ RPALNGRPN APPPSIPSQ QPPPPPPHG NHLPPPPSK TGSVKPPNH APPPPPSVL PQPPNHTPP LPPHRMVPS RAPPAVPNN FAPPPPPRN SSMTSNRKI VMDLEEKYK HMFNDPSTF PKPPPYRNV FKFYGSKQA ASKQQAPQP PNLQATTRT WDASSAC
- >TC012341 RB
MPGPPPPPP PGMMPPPPP APTSFPKAG KPDDRAALL KSIQKGTRL KKTVTNDRS APVIGGKTS NSNSSSPSG GTPASTPNG LNLGGLFAG GMPKLKPTG KLPANNVNN GKVGLPVKN SSSLQNELK KQMASDNKN RGPPPPAPV RNTPEENGT RQLSALKVG STNGLHLNQ SSLHSNLQQ QNSTLHRKV KSNANLSTL DSPDSINGF NQTSKPVIS HGKPNLAPK PPVLNAKPV APPKKLVIN GKPVGRAHS MRSPRTPSP QSPDANVAM KFGTVRNLS SVIGQSLAN STGNLSPRQ RPALNGRPN APPPSIPSQ QPPPPPPHG NHLPPPPSK TGSVKPPNH APPPPPSVL PQPPNHTPP LPPHRMVPS RAPPAVPNN FAPPPPPRN SSMTSNRKI VMDLEEKYK HMFNDPSTF PKPPPYRNV FKFYGSKQG SSC
- >TC012341 RA
atgccgggg ccgccgcca ccgccgccc cctggcatg atgccgccg ccgccgccg gccccgaca tccttcccc aaggcggga aagccggac gatcgggcg gcgctgcta aagtcgatc cagaaaggc accaggttg aagaaaacg gtgacgaat gaccgaagt gcaccggtg atagggggg aaaacgagc aattcgaac agttcgagt ccgtcaggg ggcacccca gcgtcgacg ccgaatggg ctaaattta ggcggcttg ttcgctgga ggcatgccc aagctgaag ccgacaggg aaactgcca gcgaataat gttaacaat gggaaagtg gggcttccg gtcaagaat tcgagtagt ttgcagaat gagctgaaa aagcaaatg gcaagtgat aacaaaaac cgggggccg ccaccaccg gctccggtc agaaatacg cccgaggaa aacggtaca cgtcaactg tctgcatta aaagtcggc tcaacgaac ggtttacac ctaaatcaa agttctctc cactcaaac ctccaacaa caaaacagc actctccat cgaaaagtc aaaagtaac gccaaccta agcacatta gatagtccc gactcgata aacggtttc aaccaaacc agcaaacct gtgatcagt cacggaaaa ccaaaccta gctccaaaa ccgccagta ctcaacgcc aaaccagtg gccccaccg aagaaactc gtcatcaac ggcaagccc gtcggccgc gcccacagc atgcgcagc ccccggacg ccctcgccc cagagcccc gacgccaac gtcgccatg aagttcggc acagttcgc aacctgtcg tccgtcatc ggccaatct ctggctaac tccaccgga aacctaagt cccaggcaa aggccggcg ctcaacggc cggccgaac gccccccca ccctccata ccgtcgcaa caaccgccc ccacctcct ccccatggc aaccacctg ccgccgcca ccgtcgaaa accggttcg gttaaaccg ccgaaccat gcgccgccc ccacctcct tccgtgtta cctcaaccg cccaaccat acgccgccg ctgccgccc catcggatg gtgccgtcg agggcgccg ccggcggtg ccgaacaat tttgcgccg ccgccacca ccgagaaat tcgtcgatg acgtcaaat aggaagatt gtgatggat ttggaagag aaatataag catatgttt aatgacccc agtactttt cccaaaccg ccgccctat aggaatgtt tttaaattt tacggaagc aagcaagct gctagcaaa caacaggcg ccacaaccg cccaacttg caagcaaca actcgaact tgggacgcc tcatcggcc tgctag
- >TC012341 RB
atgccgggg ccgccgcca ccgccgccc cctggcatg atgccgccg ccgccgccg gccccgaca tccttcccc aaggcggga aagccggac gatcgggcg gcgctgcta aagtcgatc cagaaaggc accaggttg aagaaaacg gtgacgaat gaccgaagt gcaccggtg atagggggg aaaacgagc aattcgaac agttcgagt ccgtcaggg ggcacccca gcgtcgacg ccgaatggg ctaaattta ggcggcttg ttcgctgga ggcatgccc aagctgaag ccgacaggg aaactgcca gcgaataat gttaacaat gggaaagtg gggcttccg gtcaagaat tcgagtagt ttgcagaat gagctgaaa aagcaaatg gcaagtgat aacaaaaac cgggggccg ccaccaccg gctccggtc agaaatacg cccgaggaa aacggtaca cgtcaactg tctgcatta aaagtcggc tcaacgaac ggtttacac ctaaatcaa agttctctc cactcaaac ctccaacaa caaaacagc actctccat cgaaaagtc aaaagtaac gccaaccta agcacatta gatagtccc gactcgata aacggtttc aaccaaacc agcaaacct gtgatcagt cacggaaaa ccaaaccta gctccaaaa ccgccagta ctcaacgcc aaaccagtg gccccaccg aagaaactc gtcatcaac ggcaagccc gtcggccgc gcccacagc atgcgcagc ccccggacg ccctcgccc cagagcccc gacgccaac gtcgccatg aagttcggc acagttcgc aacctgtcg tccgtcatc ggccaatct ctggctaac tccaccgga aacctaagt cccaggcaa aggccggcg ctcaacggc cggccgaac gccccccca ccctccata ccgtcgcaa caaccgccc ccacctcct ccccatggc aaccacctg ccgccgcca ccgtcgaaa accggttcg gttaaaccg ccgaaccat gcgccgccc ccacctcct tccgtgtta cctcaaccg cccaaccat acgccgccg ctgccgccc catcggatg gtgccgtcg agggcgccg ccggcggtg ccgaacaat tttgcgccg ccgccacca ccgagaaat tcgtcgatg acgtcaaat aggaagatt gtgatggat ttggaagag aaatataag catatgttt aatgacccc agtactttt cccaaaccg ccgccctat aggaatgtt tttaaattt tacggaagc aagcaaggc tccagttgc taa
- >TC012341 RA
tttcccaaa aacgtctat cagtggaga aagtttgaa aaaatagaa acattcctc caacttgcc cgaaataat gtgttacaa actgtattt agtgataaa gtgatgtga gatgtggtg aaggccagg ccaccagcc ttattttat ttatttatt gtcagtagt ttgtagttc aagaagcat gccggggcc gccgccacc gccgccccc tggcatgat gccgccgcc gccgccggc cccgacatc cttccccaa ggcgggaaa gccggacga tcgggcggc gctgctaaa gtcgatcca gaaaggcac caggttgaa gaaaacggt gacgaatga ccgaagtgc accggtgat aggggggaa aacgagcaa ttcgaacag ttcgagtcc gtcaggggg caccccagc gtcgacgcc gaatgggct aaatttagg cggcttgtt cgctggagg catgcccaa gctgaagcc gacagggaa actgccagc gaataatgt taacaatgg gaaagtggg gcttccggt caagaattc gagtagttt gcagaatga gctgaaaaa gcaaatggc aagtgataa caaaaaccg ggggccgcc accaccggc tccggtcag aaatacgcc cgaggaaaa cggtacacg tcaactgtc tgcattaaa agtcggctc aacgaacgg tttacacct aaatcaaag ttctctcca ctcaaacct ccaacaaca aaacagcac tctccatcg aaaagtcaa aagtaacgc caacctaag cacattaga tagtcccga ctcgataaa cggtttcaa ccaaaccag caaacctgt gatcagtca cggaaaacc aaacctagc tccaaaacc gccagtact caacgccaa accagtggc cccaccgaa gaaactcgt catcaacgg caagcccgt cggccgcgc ccacagcat gcgcagccc ccggacgcc ctcgcccca gagccccga cgccaacgt cgccatgaa gttcggcac agttcgcaa cctgtcgtc cgtcatcgg ccaatctct ggctaactc caccggaaa cctaagtcc caggcaaag gccggcgct caacggccg gccgaacgc ccccccacc ctccatacc gtcgcaaca accgccccc acctcctcc ccatggcaa ccacctgcc gccgccacc gtcgaaaac cggttcggt taaaccgcc gaaccatgc gccgccccc acctccttc cgtgttacc tcaaccgcc caaccatac gccgccgct gccgcccca tcggatggt gccgtcgag ggcgccgcc ggcggtgcc gaacaattt tgcgccgcc gccaccacc gagaaattc gtcgatgac gtcaaatag gaagattgt gatggattt ggaagagaa atataagca tatgtttaa tgaccccag tacttttcc caaaccgcc gccctatag gaatgtttt taaatttta cggaagcaa gcaagctgc tagcaaaca acaggcgcc acaaccgcc caacttgca agcaacaac tcgaacttg ggacgcctc atcggcctg ctagcacca aatttccta aaaaattat tgttgctca agtgtgtgc aattgtcat atcagtaat tcgtttccg gtggactcc aaaatatag ctttatata ttataataa aattacgac tggattgtt gaacaaagt gctttatgt ttgaacagg caagctgtt gatatgttg cgaaatgat tgaacaata ttgttagtc tcgttggct gtggatttt tctgtaggg tgatttttg tttttcatt ttccattgt cgagagttg tagcaagac tgcctagaa cgaatgaga tatttttct agcagtatt tcgaattgg aagaattac cacgtattg aagacttga ccgtacctc catttacgt tttctaagt ggtcaggga ttcactttg atcgcaaat tcaaataaa ggcaccgta ttagtcaag tgaccggtt taatgtatt ttttattag tcatttatt tgtttaaag taacacttg ttatagttt tcacgtatc tgttaataa tttaaaaaa ccaaatcta atatagtag ggtttaaat ctaataaac actaaatta ggtacttat atccttaag tattgtgct acttttgag gcgaatatg accgacgtt caaacatat ttgctcttt tagcatttc attttttat taatttatt tatcgtttg tattttatt tgtagcctt atcttctta ttagtattt tttattatt tcgtatttt acatacttt tctctagat tttaaaatt gttattgct agtcacaat aatatcttc cattagcaa atatttcag tcagtgcct aaataatct ttaaatgta tatacagtt gtaaatata atgtacaaa attataata aaagagtaa tattcta
- >TC012341 RB
tttcccaaa aacgtctat cagtggaga aagtttgaa aaaatagaa acattcctc caacttgcc cgaaataat gtgttacaa actgtattt agtgataaa gtgatgtga gatgtggtg aaggccagg ccaccagcc ttattttat ttatttatt gtcagtagt ttgtagttc aagaagcat gccggggcc gccgccacc gccgccccc tggcatgat gccgccgcc gccgccggc cccgacatc cttccccaa ggcgggaaa gccggacga tcgggcggc gctgctaaa gtcgatcca gaaaggcac caggttgaa gaaaacggt gacgaatga ccgaagtgc accggtgat aggggggaa aacgagcaa ttcgaacag ttcgagtcc gtcaggggg caccccagc gtcgacgcc gaatgggct aaatttagg cggcttgtt cgctggagg catgcccaa gctgaagcc gacagggaa actgccagc gaataatgt taacaatgg gaaagtggg gcttccggt caagaattc gagtagttt gcagaatga gctgaaaaa gcaaatggc aagtgataa caaaaaccg ggggccgcc accaccggc tccggtcag aaatacgcc cgaggaaaa cggtacacg tcaactgtc tgcattaaa agtcggctc aacgaacgg tttacacct aaatcaaag ttctctcca ctcaaacct ccaacaaca aaacagcac tctccatcg aaaagtcaa aagtaacgc caacctaag cacattaga tagtcccga ctcgataaa cggtttcaa ccaaaccag caaacctgt gatcagtca cggaaaacc aaacctagc tccaaaacc gccagtact caacgccaa accagtggc cccaccgaa gaaactcgt catcaacgg caagcccgt cggccgcgc ccacagcat gcgcagccc ccggacgcc ctcgcccca gagccccga cgccaacgt cgccatgaa gttcggcac agttcgcaa cctgtcgtc cgtcatcgg ccaatctct ggctaactc caccggaaa cctaagtcc caggcaaag gccggcgct caacggccg gccgaacgc ccccccacc ctccatacc gtcgcaaca accgccccc acctcctcc ccatggcaa ccacctgcc gccgccacc gtcgaaaac cggttcggt taaaccgcc gaaccatgc gccgccccc acctccttc cgtgttacc tcaaccgcc caaccatac gccgccgct gccgcccca tcggatggt gccgtcgag ggcgccgcc ggcggtgcc gaacaattt tgcgccgcc gccaccacc gagaaattc gtcgatgac gtcaaatag gaagattgt gatggattt ggaagagaa atataagca tatgtttaa tgaccccag tacttttcc caaaccgcc gccctatag gaatgtttt taaatttta cggaagcaa gcaaggctc cagttgcta aacggactt gcagctgca tgttcacca ttgccattg catgaattc agtcgacta ctaacaaca ttatttact ggtgacttt tgtttaatt cgaacactt ttttatttt tctgtaaat acgtttcat tttcttatt tccataatg ttacttaat gggaatgtt ttgtttgtg ttgttttgt gcaatagtt gtatattgc ttcctacgc taacataag cttattgcg taaaatgtg gcaaatgtg attttgtcg ctttttgcc gaaaaataa cgttgtgaa ttattgtag ctgctagca aacaacagg cgccacaac cgcccaact tgcaagcaa caactcgaa cttgggacg cctcatcgg cctgctagc accaaattt cctaaaaaa ttattgttg ctcaagtgt gtgcaattg tcatatcag taattcgtt tccggtgga ctccaaaat atagcttta tatattata ataaaatta cgactggat tgttgaaca aagtgcttt atgtttgaa caggcaagc tgttgatat gttgcgaaa tgattgaac aatattgtt agtctcgtt ggctgtgga tttttctgt agggtgatt tttgttttt cattttcca ttgtcgaga gttgtagca agactgcct agaacgaat gagatattt ttctagcag tatttcgaa ttggaagaa ttaccacgt attgaagac ttgaccgta cctccattt acgttttct aagtggtca gggattcac tttgatcgc aaattcaaa taaaggcac cgtattagt caagtgacc ggtttaatg tatttttta ttagtcatt tatttgttt aaagtaaca cttgttata gttttcacg tatctgtta ataatttaa aaaaccaaa tctaatata gtagggttt aaatctaat aaacactaa attaggtac ttatatcct taagtattg tgctacttt tgaggcgaa tatgaccga cgttcaaac atatttgct cttttagca tttcatttt ttattaatt tatttatcg tttgtattt tatttgtag ccttatctt cttattagt attttttat tatttcgta ttttacata cttttctct agattttaa aattgttat tgctagtca caataatat cttccatta gcaaatatt tcagtcagt gcctaaata atctttaaa tgtatatac agttgtaaa tataatgta caaaattat aataaaaga gtaatattc ta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
