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Gene information
General information
TC012341 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5074786..5084285
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012341
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05218

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 4 - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012341 RA
      MPGPPPPPP PGMMPPPPP APTSFPKAG KPDDRAALL 
      KSIQKGTRL KKTVTNDRS APVIGGKTS NSNSSSPSG 
      GTPASTPNG LNLGGLFAG GMPKLKPTG KLPANNVNN 
      GKVGLPVKN SSSLQNELK KQMASDNKN RGPPPPAPV 
      RNTPEENGT RQLSALKVG STNGLHLNQ SSLHSNLQQ 
      QNSTLHRKV KSNANLSTL DSPDSINGF NQTSKPVIS 
      HGKPNLAPK PPVLNAKPV APPKKLVIN GKPVGRAHS 
      MRSPRTPSP QSPDANVAM KFGTVRNLS SVIGQSLAN 
      STGNLSPRQ RPALNGRPN APPPSIPSQ QPPPPPPHG 
      NHLPPPPSK TGSVKPPNH APPPPPSVL PQPPNHTPP 
      LPPHRMVPS RAPPAVPNN FAPPPPPRN SSMTSNRKI 
      VMDLEEKYK HMFNDPSTF PKPPPYRNV FKFYGSKQA 
      ASKQQAPQP PNLQATTRT WDASSAC
    • >TC012341 RB
      MPGPPPPPP PGMMPPPPP APTSFPKAG KPDDRAALL 
      KSIQKGTRL KKTVTNDRS APVIGGKTS NSNSSSPSG 
      GTPASTPNG LNLGGLFAG GMPKLKPTG KLPANNVNN 
      GKVGLPVKN SSSLQNELK KQMASDNKN RGPPPPAPV 
      RNTPEENGT RQLSALKVG STNGLHLNQ SSLHSNLQQ 
      QNSTLHRKV KSNANLSTL DSPDSINGF NQTSKPVIS 
      HGKPNLAPK PPVLNAKPV APPKKLVIN GKPVGRAHS 
      MRSPRTPSP QSPDANVAM KFGTVRNLS SVIGQSLAN 
      STGNLSPRQ RPALNGRPN APPPSIPSQ QPPPPPPHG 
      NHLPPPPSK TGSVKPPNH APPPPPSVL PQPPNHTPP 
      LPPHRMVPS RAPPAVPNN FAPPPPPRN SSMTSNRKI 
      VMDLEEKYK HMFNDPSTF PKPPPYRNV FKFYGSKQG 
      SSC
    CDS sequence
    • >TC012341 RA
      atgccgggg ccgccgcca ccgccgccc cctggcatg 
      atgccgccg ccgccgccg gccccgaca tccttcccc 
      aaggcggga aagccggac gatcgggcg gcgctgcta 
      aagtcgatc cagaaaggc accaggttg aagaaaacg 
      gtgacgaat gaccgaagt gcaccggtg atagggggg 
      aaaacgagc aattcgaac agttcgagt ccgtcaggg 
      ggcacccca gcgtcgacg ccgaatggg ctaaattta 
      ggcggcttg ttcgctgga ggcatgccc aagctgaag 
      ccgacaggg aaactgcca gcgaataat gttaacaat 
      gggaaagtg gggcttccg gtcaagaat tcgagtagt 
      ttgcagaat gagctgaaa aagcaaatg gcaagtgat 
      aacaaaaac cgggggccg ccaccaccg gctccggtc 
      agaaatacg cccgaggaa aacggtaca cgtcaactg 
      tctgcatta aaagtcggc tcaacgaac ggtttacac 
      ctaaatcaa agttctctc cactcaaac ctccaacaa 
      caaaacagc actctccat cgaaaagtc aaaagtaac 
      gccaaccta agcacatta gatagtccc gactcgata 
      aacggtttc aaccaaacc agcaaacct gtgatcagt 
      cacggaaaa ccaaaccta gctccaaaa ccgccagta 
      ctcaacgcc aaaccagtg gccccaccg aagaaactc 
      gtcatcaac ggcaagccc gtcggccgc gcccacagc 
      atgcgcagc ccccggacg ccctcgccc cagagcccc 
      gacgccaac gtcgccatg aagttcggc acagttcgc 
      aacctgtcg tccgtcatc ggccaatct ctggctaac 
      tccaccgga aacctaagt cccaggcaa aggccggcg 
      ctcaacggc cggccgaac gccccccca ccctccata 
      ccgtcgcaa caaccgccc ccacctcct ccccatggc 
      aaccacctg ccgccgcca ccgtcgaaa accggttcg 
      gttaaaccg ccgaaccat gcgccgccc ccacctcct 
      tccgtgtta cctcaaccg cccaaccat acgccgccg 
      ctgccgccc catcggatg gtgccgtcg agggcgccg 
      ccggcggtg ccgaacaat tttgcgccg ccgccacca 
      ccgagaaat tcgtcgatg acgtcaaat aggaagatt 
      gtgatggat ttggaagag aaatataag catatgttt 
      aatgacccc agtactttt cccaaaccg ccgccctat 
      aggaatgtt tttaaattt tacggaagc aagcaagct 
      gctagcaaa caacaggcg ccacaaccg cccaacttg 
      caagcaaca actcgaact tgggacgcc tcatcggcc 
      tgctag
    • >TC012341 RB
      atgccgggg ccgccgcca ccgccgccc cctggcatg 
      atgccgccg ccgccgccg gccccgaca tccttcccc 
      aaggcggga aagccggac gatcgggcg gcgctgcta 
      aagtcgatc cagaaaggc accaggttg aagaaaacg 
      gtgacgaat gaccgaagt gcaccggtg atagggggg 
      aaaacgagc aattcgaac agttcgagt ccgtcaggg 
      ggcacccca gcgtcgacg ccgaatggg ctaaattta 
      ggcggcttg ttcgctgga ggcatgccc aagctgaag 
      ccgacaggg aaactgcca gcgaataat gttaacaat 
      gggaaagtg gggcttccg gtcaagaat tcgagtagt 
      ttgcagaat gagctgaaa aagcaaatg gcaagtgat 
      aacaaaaac cgggggccg ccaccaccg gctccggtc 
      agaaatacg cccgaggaa aacggtaca cgtcaactg 
      tctgcatta aaagtcggc tcaacgaac ggtttacac 
      ctaaatcaa agttctctc cactcaaac ctccaacaa 
      caaaacagc actctccat cgaaaagtc aaaagtaac 
      gccaaccta agcacatta gatagtccc gactcgata 
      aacggtttc aaccaaacc agcaaacct gtgatcagt 
      cacggaaaa ccaaaccta gctccaaaa ccgccagta 
      ctcaacgcc aaaccagtg gccccaccg aagaaactc 
      gtcatcaac ggcaagccc gtcggccgc gcccacagc 
      atgcgcagc ccccggacg ccctcgccc cagagcccc 
      gacgccaac gtcgccatg aagttcggc acagttcgc 
      aacctgtcg tccgtcatc ggccaatct ctggctaac 
      tccaccgga aacctaagt cccaggcaa aggccggcg 
      ctcaacggc cggccgaac gccccccca ccctccata 
      ccgtcgcaa caaccgccc ccacctcct ccccatggc 
      aaccacctg ccgccgcca ccgtcgaaa accggttcg 
      gttaaaccg ccgaaccat gcgccgccc ccacctcct 
      tccgtgtta cctcaaccg cccaaccat acgccgccg 
      ctgccgccc catcggatg gtgccgtcg agggcgccg 
      ccggcggtg ccgaacaat tttgcgccg ccgccacca 
      ccgagaaat tcgtcgatg acgtcaaat aggaagatt 
      gtgatggat ttggaagag aaatataag catatgttt 
      aatgacccc agtactttt cccaaaccg ccgccctat 
      aggaatgtt tttaaattt tacggaagc aagcaaggc 
      tccagttgc taa
    mRNA sequence
    • >TC012341 RA
      tttcccaaa aacgtctat cagtggaga aagtttgaa 
      aaaatagaa acattcctc caacttgcc cgaaataat 
      gtgttacaa actgtattt agtgataaa gtgatgtga 
      gatgtggtg aaggccagg ccaccagcc ttattttat 
      ttatttatt gtcagtagt ttgtagttc aagaagcat 
      gccggggcc gccgccacc gccgccccc tggcatgat 
      gccgccgcc gccgccggc cccgacatc cttccccaa 
      ggcgggaaa gccggacga tcgggcggc gctgctaaa 
      gtcgatcca gaaaggcac caggttgaa gaaaacggt 
      gacgaatga ccgaagtgc accggtgat aggggggaa 
      aacgagcaa ttcgaacag ttcgagtcc gtcaggggg 
      caccccagc gtcgacgcc gaatgggct aaatttagg 
      cggcttgtt cgctggagg catgcccaa gctgaagcc 
      gacagggaa actgccagc gaataatgt taacaatgg 
      gaaagtggg gcttccggt caagaattc gagtagttt 
      gcagaatga gctgaaaaa gcaaatggc aagtgataa 
      caaaaaccg ggggccgcc accaccggc tccggtcag 
      aaatacgcc cgaggaaaa cggtacacg tcaactgtc 
      tgcattaaa agtcggctc aacgaacgg tttacacct 
      aaatcaaag ttctctcca ctcaaacct ccaacaaca 
      aaacagcac tctccatcg aaaagtcaa aagtaacgc 
      caacctaag cacattaga tagtcccga ctcgataaa 
      cggtttcaa ccaaaccag caaacctgt gatcagtca 
      cggaaaacc aaacctagc tccaaaacc gccagtact 
      caacgccaa accagtggc cccaccgaa gaaactcgt 
      catcaacgg caagcccgt cggccgcgc ccacagcat 
      gcgcagccc ccggacgcc ctcgcccca gagccccga 
      cgccaacgt cgccatgaa gttcggcac agttcgcaa 
      cctgtcgtc cgtcatcgg ccaatctct ggctaactc 
      caccggaaa cctaagtcc caggcaaag gccggcgct 
      caacggccg gccgaacgc ccccccacc ctccatacc 
      gtcgcaaca accgccccc acctcctcc ccatggcaa 
      ccacctgcc gccgccacc gtcgaaaac cggttcggt 
      taaaccgcc gaaccatgc gccgccccc acctccttc 
      cgtgttacc tcaaccgcc caaccatac gccgccgct 
      gccgcccca tcggatggt gccgtcgag ggcgccgcc 
      ggcggtgcc gaacaattt tgcgccgcc gccaccacc 
      gagaaattc gtcgatgac gtcaaatag gaagattgt 
      gatggattt ggaagagaa atataagca tatgtttaa 
      tgaccccag tacttttcc caaaccgcc gccctatag 
      gaatgtttt taaatttta cggaagcaa gcaagctgc 
      tagcaaaca acaggcgcc acaaccgcc caacttgca 
      agcaacaac tcgaacttg ggacgcctc atcggcctg 
      ctagcacca aatttccta aaaaattat tgttgctca 
      agtgtgtgc aattgtcat atcagtaat tcgtttccg 
      gtggactcc aaaatatag ctttatata ttataataa 
      aattacgac tggattgtt gaacaaagt gctttatgt 
      ttgaacagg caagctgtt gatatgttg cgaaatgat 
      tgaacaata ttgttagtc tcgttggct gtggatttt 
      tctgtaggg tgatttttg tttttcatt ttccattgt 
      cgagagttg tagcaagac tgcctagaa cgaatgaga 
      tatttttct agcagtatt tcgaattgg aagaattac 
      cacgtattg aagacttga ccgtacctc catttacgt 
      tttctaagt ggtcaggga ttcactttg atcgcaaat 
      tcaaataaa ggcaccgta ttagtcaag tgaccggtt 
      taatgtatt ttttattag tcatttatt tgtttaaag 
      taacacttg ttatagttt tcacgtatc tgttaataa 
      tttaaaaaa ccaaatcta atatagtag ggtttaaat 
      ctaataaac actaaatta ggtacttat atccttaag 
      tattgtgct acttttgag gcgaatatg accgacgtt 
      caaacatat ttgctcttt tagcatttc attttttat 
      taatttatt tatcgtttg tattttatt tgtagcctt 
      atcttctta ttagtattt tttattatt tcgtatttt 
      acatacttt tctctagat tttaaaatt gttattgct 
      agtcacaat aatatcttc cattagcaa atatttcag 
      tcagtgcct aaataatct ttaaatgta tatacagtt 
      gtaaatata atgtacaaa attataata aaagagtaa 
      tattcta
    • >TC012341 RB
      tttcccaaa aacgtctat cagtggaga aagtttgaa 
      aaaatagaa acattcctc caacttgcc cgaaataat 
      gtgttacaa actgtattt agtgataaa gtgatgtga 
      gatgtggtg aaggccagg ccaccagcc ttattttat 
      ttatttatt gtcagtagt ttgtagttc aagaagcat 
      gccggggcc gccgccacc gccgccccc tggcatgat 
      gccgccgcc gccgccggc cccgacatc cttccccaa 
      ggcgggaaa gccggacga tcgggcggc gctgctaaa 
      gtcgatcca gaaaggcac caggttgaa gaaaacggt 
      gacgaatga ccgaagtgc accggtgat aggggggaa 
      aacgagcaa ttcgaacag ttcgagtcc gtcaggggg 
      caccccagc gtcgacgcc gaatgggct aaatttagg 
      cggcttgtt cgctggagg catgcccaa gctgaagcc 
      gacagggaa actgccagc gaataatgt taacaatgg 
      gaaagtggg gcttccggt caagaattc gagtagttt 
      gcagaatga gctgaaaaa gcaaatggc aagtgataa 
      caaaaaccg ggggccgcc accaccggc tccggtcag 
      aaatacgcc cgaggaaaa cggtacacg tcaactgtc 
      tgcattaaa agtcggctc aacgaacgg tttacacct 
      aaatcaaag ttctctcca ctcaaacct ccaacaaca 
      aaacagcac tctccatcg aaaagtcaa aagtaacgc 
      caacctaag cacattaga tagtcccga ctcgataaa 
      cggtttcaa ccaaaccag caaacctgt gatcagtca 
      cggaaaacc aaacctagc tccaaaacc gccagtact 
      caacgccaa accagtggc cccaccgaa gaaactcgt 
      catcaacgg caagcccgt cggccgcgc ccacagcat 
      gcgcagccc ccggacgcc ctcgcccca gagccccga 
      cgccaacgt cgccatgaa gttcggcac agttcgcaa 
      cctgtcgtc cgtcatcgg ccaatctct ggctaactc 
      caccggaaa cctaagtcc caggcaaag gccggcgct 
      caacggccg gccgaacgc ccccccacc ctccatacc 
      gtcgcaaca accgccccc acctcctcc ccatggcaa 
      ccacctgcc gccgccacc gtcgaaaac cggttcggt 
      taaaccgcc gaaccatgc gccgccccc acctccttc 
      cgtgttacc tcaaccgcc caaccatac gccgccgct 
      gccgcccca tcggatggt gccgtcgag ggcgccgcc 
      ggcggtgcc gaacaattt tgcgccgcc gccaccacc 
      gagaaattc gtcgatgac gtcaaatag gaagattgt 
      gatggattt ggaagagaa atataagca tatgtttaa 
      tgaccccag tacttttcc caaaccgcc gccctatag 
      gaatgtttt taaatttta cggaagcaa gcaaggctc 
      cagttgcta aacggactt gcagctgca tgttcacca 
      ttgccattg catgaattc agtcgacta ctaacaaca 
      ttatttact ggtgacttt tgtttaatt cgaacactt 
      ttttatttt tctgtaaat acgtttcat tttcttatt 
      tccataatg ttacttaat gggaatgtt ttgtttgtg 
      ttgttttgt gcaatagtt gtatattgc ttcctacgc 
      taacataag cttattgcg taaaatgtg gcaaatgtg 
      attttgtcg ctttttgcc gaaaaataa cgttgtgaa 
      ttattgtag ctgctagca aacaacagg cgccacaac 
      cgcccaact tgcaagcaa caactcgaa cttgggacg 
      cctcatcgg cctgctagc accaaattt cctaaaaaa 
      ttattgttg ctcaagtgt gtgcaattg tcatatcag 
      taattcgtt tccggtgga ctccaaaat atagcttta 
      tatattata ataaaatta cgactggat tgttgaaca 
      aagtgcttt atgtttgaa caggcaagc tgttgatat 
      gttgcgaaa tgattgaac aatattgtt agtctcgtt 
      ggctgtgga tttttctgt agggtgatt tttgttttt 
      cattttcca ttgtcgaga gttgtagca agactgcct 
      agaacgaat gagatattt ttctagcag tatttcgaa 
      ttggaagaa ttaccacgt attgaagac ttgaccgta 
      cctccattt acgttttct aagtggtca gggattcac 
      tttgatcgc aaattcaaa taaaggcac cgtattagt 
      caagtgacc ggtttaatg tatttttta ttagtcatt 
      tatttgttt aaagtaaca cttgttata gttttcacg 
      tatctgtta ataatttaa aaaaccaaa tctaatata 
      gtagggttt aaatctaat aaacactaa attaggtac 
      ttatatcct taagtattg tgctacttt tgaggcgaa 
      tatgaccga cgttcaaac atatttgct cttttagca 
      tttcatttt ttattaatt tatttatcg tttgtattt 
      tatttgtag ccttatctt cttattagt attttttat 
      tatttcgta ttttacata cttttctct agattttaa 
      aattgttat tgctagtca caataatat cttccatta 
      gcaaatatt tcagtcagt gcctaaata atctttaaa 
      tgtatatac agttgtaaa tataatgta caaaattat 
      aataaaaga gtaatattc ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY