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Gene information
General information
TC034297
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012340
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5064758..5071056
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034297
    iBeetle screen iB_05217

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    pupal molt not completely molted - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034297 RA
      MSDREGSRS PIDRKAMID RDHSPHARR EESPRNHKA 
      KSYSRSPSR SRKISYRDG DRDRERRRD DRDREDRRY 
      RSRSRSRSD RRYKSSRYS RSHSRSPGD RRSLSHSPM 
      SSRRRHLGT RDNPKPSRC LGVFGLSVY TTEDELYHI 
      FSKYGPLER VQVVIDAKT GRSRGFSFV YFENTDDAK 
      VAKDQCSGM KINGKNIRV DYSITERAH TPTPGIYMG 
      KPTYKYNDR YDRRRDRDD YYGRGSRYS HRRSPSPYY 
      RRRRYDRSR SRSYSPRRY
    • >TC034297 RB
      MSDREGSRS PIDRKAMID RDHSPHARR EESPRNHKA 
      KSYSRSPSR SRKISYRDG DRDRERRRD DRDREDRRY 
      RSRSRSRSD RRYKSSRYS RSHSRSPGD RRSLSHSPM 
      SSRRRHLGT RDNPKPSRC LGVFGLSVY TTEDELYHI 
      FSKYGPLER VQVVIDAKT GRSRGFSFV YFENTDDAK 
      VAKDQCSGM KINGKNIRV DYSITERAH TPTPGIYMG 
      KPTYKYNDR YDRRRDRDD YYGRGSRYS HRRSPSPYY 
      RRRRYDRSR SRSYSPRTQ RI
    CDS sequence
    • >TC034297 RA
      atgagtgat agggagggg agccgctcg cccattgat 
      cgtaaagca atgatcgac cgagaccac tctccacac 
      gcgaggcgg gaagagtct ccaagaaat cacaaggcc 
      aagagttac tcaaggagt ccaagccgg agtagaaaa 
      atatcgtat cgagatggc gacagagac cgagaaagg 
      cgccgcgac gaccgagac cgagaagat cgcagatac 
      aggtcgcgc agtcgatca cgctcagac agacgttac 
      aagtcctcg agatattca cgtagtcac tcgcgttcc 
      cccggcgac cggcggtcg ctctcacat agccctatg 
      tctagccgc aggcgccat ttggggacc agggacaat 
      ccaaaacct tcgcggtgt cttggcgtt ttcggattg 
      agcgtctac acgacagag gacgaacta tatcacatc 
      ttttccaaa tatgggccg cttgaaagg gtgcaagtt 
      gtcattgat gcaaagact ggacgctca cgaggcttc 
      tcgtttgtg tattttgaa aatacggac gatgcgaaa 
      gtagccaaa gatcaatgt tctggtatg aaaataaac 
      ggtaaaaat attcgagtc gattattct atcacagaa 
      agggcgcac acaccgacg cctggaata tacatggga 
      aaacccacc tataaatat aatgatcgt tacgatcga 
      cgccgggat agagatgat tattatggc cgtggttcg 
      cgctacagc catagacgt tctccatcg ccgtattac 
      aggcgacgt cgttacgac cgttcgaga tctcgcagt 
      tactcgcct cgtcgttat taa
    • >TC034297 RB
      atgagtgat agggagggg agccgctcg cccattgat 
      cgtaaagca atgatcgac cgagaccac tctccacac 
      gcgaggcgg gaagagtct ccaagaaat cacaaggcc 
      aagagttac tcaaggagt ccaagccgg agtagaaaa 
      atatcgtat cgagatggc gacagagac cgagaaagg 
      cgccgcgac gaccgagac cgagaagat cgcagatac 
      aggtcgcgc agtcgatca cgctcagac agacgttac 
      aagtcctcg agatattca cgtagtcac tcgcgttcc 
      cccggcgac cggcggtcg ctctcacat agccctatg 
      tctagccgc aggcgccat ttggggacc agggacaat 
      ccaaaacct tcgcggtgt cttggcgtt ttcggattg 
      agcgtctac acgacagag gacgaacta tatcacatc 
      ttttccaaa tatgggccg cttgaaagg gtgcaagtt 
      gtcattgat gcaaagact ggacgctca cgaggcttc 
      tcgtttgtg tattttgaa aatacggac gatgcgaaa 
      gtagccaaa gatcaatgt tctggtatg aaaataaac 
      ggtaaaaat attcgagtc gattattct atcacagaa 
      agggcgcac acaccgacg cctggaata tacatggga 
      aaacccacc tataaatat aatgatcgt tacgatcga 
      cgccgggat agagatgat tattatggc cgtggttcg 
      cgctacagc catagacgt tctccatcg ccgtattac 
      aggcgacgt cgttacgac cgttcgaga tctcgcagt 
      tactcgcct cgtacccag agaatttga
    mRNA sequence
    • >TC034297 RA
      agcgagtaa acagaagaa cttccgtgc gacagcagc 
      gcctctttg ccattcgcc gccatcatt ccacaagtt 
      ttgtttggc gtgtttgtg gtcaagttg cgtttaaga 
      ctaaaaaac tcactagtt gtcctattt tcttccaat 
      ttcctagta tttatttta ttttttact atgagtgat 
      agggagggg agccgctcg cccattgat cgtaaagca 
      atgatcgac cgagaccac tctccacac gcgaggcgg 
      gaagagtct ccaagaaat cacaaggcc aagagttac 
      tcaaggagt ccaagccgg agtagaaaa atatcgtat 
      cgagatggc gacagagac cgagaaagg cgccgcgac 
      gaccgagac cgagaagat cgcagatac aggtcgcgc 
      agtcgatca cgctcagac agacgttac aagtcctcg 
      agatattca cgtagtcac tcgcgttcc cccggcgac 
      cggcggtcg ctctcacat agccctatg tctagccgc 
      aggcgccat ttggggacc agggacaat ccaaaacct 
      tcgcggtgt cttggcgtt ttcggattg agcgtctac 
      acgacagag gacgaacta tatcacatc ttttccaaa 
      tatgggccg cttgaaagg gtgcaagtt gtcattgat 
      gcaaagact ggacgctca cgaggcttc tcgtttgtg 
      tattttgaa aatacggac gatgcgaaa gtagccaaa 
      gatcaatgt tctggtatg aaaataaac ggtaaaaat 
      attcgagtc gattattct atcacagaa agggcgcac 
      acaccgacg cctggaata tacatggga aaacccacc 
      tataaatat aatgatcgt tacgatcga cgccgggat 
      agagatgat tattatggc cgtggttcg cgctacagc 
      catagacgt tctccatcg ccgtattac aggcgacgt 
      cgttacgac cgttcgaga tctcgcagt tactcgcct 
      cgtcgttat taaacttcg tgggggcac tgcatttat 
      tggagagat aaaccgtag ttgtaacat ttcggtgga 
      gtttatgcc agtattgta gatgtatat tttttacat 
      ttggttttt aatgtatga tgttcctgg tgtaatctc 
      catgcgtta ttttattgg tttattttt catattcgc 
      gcgtttgag agaatgtcg gcgggtttt aagcgccat 
      cgtggattt tgtataact gattcgaaa cttgatgcc 
      aatcgaatg gttgaaagt tttgatatg tagtgtagt 
      ttttgtttg ttctttctt ggttttctt gagttcaat 
      ctgtgacac ttagataag ctctaataa atgattgat 
      atcaacact aatatcgtt ttctttgtt taaaaaggg 
      ataagaaca agaggtttg ggatgaatt atgaagttt 
      atataagaa atagaattt tctcaagtt accttaaat 
      cacttctct gaagcttat ttcaatgaa tcagctaaa 
      cacacgaaa tgaattgtg aaaatacat ccatcaaga 
      gaattgaga aatttttaa caactctct tgaagaatg 
      aaaaattgt tttcttttt ttaagtaga agacacctc 
      tcattgaaa atcagctct tagtcccac caaggcgat 
      ttgcattgt tcactcaga ccgaaaatc tgctcccaa 
      ttagatcaa aacttgaaa tttgaaaat gtaggagtt 
      tggtacgta gaacgccaa atatacaaa ctcaagact 
      ttcttatct actctgttc aacagattc acagtaaca 
      gttttcact taatctata tgtattttt tgaatattg 
      ttagacata atgcatgtc actgtattc tttcagatt 
      catgttaaa cagcttata ctaactttt ttagtcatt 
      tactaatgt gcacattca gttgtttta gtataaggt 
      gaattggat ttttttaga gttaagcct tcatctact 
      cgtggagta gaaatacat atagatttt cgctgtaac 
      cctccagtt gcagttaca taaattttt ttttcctca 
      ataggtacc cagagaatt tgaagattt gcagcgagt 
      tgtacaaac gtcttattg taccaatag cagtaatga 
      ataaattgt ccaaacata a
    • >TC034297 RB
      agcgagtaa acagaagaa cttccgtgc gacagcagc 
      gcctctttg ccattcgcc gccatcatt ccacaagtt 
      ttgtttggc gtgtttgtg gtcaagttg cgtttaaga 
      ctaaaaaac tcactagtt gtcctattt tcttccaat 
      ttcctagta tttatttta ttttttact atgagtgat 
      agggagggg agccgctcg cccattgat cgtaaagca 
      atgatcgac cgagaccac tctccacac gcgaggcgg 
      gaagagtct ccaagaaat cacaaggcc aagagttac 
      tcaaggagt ccaagccgg agtagaaaa atatcgtat 
      cgagatggc gacagagac cgagaaagg cgccgcgac 
      gaccgagac cgagaagat cgcagatac aggtcgcgc 
      agtcgatca cgctcagac agacgttac aagtcctcg 
      agatattca cgtagtcac tcgcgttcc cccggcgac 
      cggcggtcg ctctcacat agccctatg tctagccgc 
      aggcgccat ttggggacc agggacaat ccaaaacct 
      tcgcggtgt cttggcgtt ttcggattg agcgtctac 
      acgacagag gacgaacta tatcacatc ttttccaaa 
      tatgggccg cttgaaagg gtgcaagtt gtcattgat 
      gcaaagact ggacgctca cgaggcttc tcgtttgtg 
      tattttgaa aatacggac gatgcgaaa gtagccaaa 
      gatcaatgt tctggtatg aaaataaac ggtaaaaat 
      attcgagtc gattattct atcacagaa agggcgcac 
      acaccgacg cctggaata tacatggga aaacccacc 
      tataaatat aatgatcgt tacgatcga cgccgggat 
      agagatgat tattatggc cgtggttcg cgctacagc 
      catagacgt tctccatcg ccgtattac aggcgacgt 
      cgttacgac cgttcgaga tctcgcagt tactcgcct 
      cgtacccag agaatttga agatttgca gcgagttgt 
      acaaacgtc ttattgtac caatagcag taatgaata 
      aattgtcca aacataa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY