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Gene information
General information
TC012326 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4862639..4864656
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012326
    iBeetle screen iB_05214

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 33.33% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Pupal morphology
    wings slightly shortened - Number of animals showing the phenotype: 8

    wings orientation irregular - Number of animals showing the phenotype: 6
    elytra distal thinner - Number of animals showing the phenotype: 6
    Adult morphology
    wings shape irregular - Number of animals showing the phenotype: 5


    elytra distal width decreased - Number of animals showing the phenotype: 5

    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovariole mostly not present - Number of animals showing the phenotype: 1

    tropharium size decreased - Number of animals showing the phenotype: 2

    tropharium shape rounded - Number of animals showing the phenotype: 2
    vitellogenic egg chamber early number increased - Number of animals showing the phenotype: 2
    tropharium mostly not present - Number of animals showing the phenotype: 1

    vitellarium mostly not present - Number of animals showing the phenotype: 1
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 1
    vitellogenic egg chamber early ectopically- Number of animals showing the phenotype: 1
    ovariole shape irregular - Number of animals showing the phenotype: 1
    ovariole sometimes mostlynot present - Number of animals showing the phenotype: 1
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012326 RA
      MALGKETER SLIQKYVTI FLVVSGYWV VSILTVFIN 
      KTLLSDISL DAPMFIALY QTFITAAIC FVKKGLARV 
      FPQHFSFPE TSVWDAQTI KTILPVSLM FTMMIAMNN 
      LCLKYVSVA FYYIGRSLT TIFNVAFTF VILGERTSK 
      QCLFFCGVI IFGFYLGVD QESLSGSLS ISGTIFGVL 
      ASLSLSLYS IFTKKVLTK VDNQVWALS YYNNIYATI 
      LFIPLMLFN NEFFVLADY SGLSQTYFW FIMTVGGVC 
      GFAIGFFTS LQIKYTSAL THNISGTAK ACAQTVLAT 
      YWYQEAKSA LWWCSNFIV LLGSMGYAR VKQLDMEKK 
      HKETMMYQR V
    CDS sequence
    • >TC012326 RA
      atggcacta gggaaggaa actgagagg agtttaatt 
      cagaaatat gttacaata tttcttgta gttagtggc 
      tattgggtt gtctcgatt ttgactgtg tttatcaac 
      aaaaccctc ctcagtgac atcagttta gacgccccg 
      atgttcatt gcgttgtat caaaccttc ataaccgca 
      gccatttgt ttcgttaaa aaaggctta gctcgggta 
      ttcccccaa cattttagt ttccctgaa acgagcgta 
      tgggacgca caaacaatt aaaacaatc ttgcccgtg 
      tctcttatg tttactatg atgatcgca atgaacaat 
      ctctgctta aaatacgtc tcagtcgct ttctactac 
      attggaagg tccttgacc acgattttt aacgtagcg 
      ttcacgttc gtaatcttg ggggagaga acgtccaag 
      cagtgttta tttttttgt ggtgtcatc atatttggg 
      ttttactta ggagtggac caggaaagt ctctctggg 
      agtttatca ataagtggc accattttt ggtgttttg 
      gcttcgctg tctttatcc ctttattca attttcact 
      aaaaaagtt ctgacaaaa gttgataac caagtttgg 
      gcgttatcg tactacaac aatatttat gcaactatt 
      ttattcatt cccttgatg ctttttaat aacgaattc 
      tttgtcttg gccgattac agtgggtta tcacaaaca 
      tatttttgg tttataatg acagttggg ggagtttgt 
      ggattcgca attggattt tttacctct ttacaaatc 
      aagtatacg tcagcttta acgcacaac atatcaggc 
      acagctaag gcttgtgcc caaactgtg ttagccact 
      tactggtat caagaagca aagtcggca ctttggtgg 
      tgttcaaac tttattgtc ttgttggga agcatggga 
      tatgcaaga gttaaacaa cttgatatg gagaaaaaa 
      cataaagag accatgatg tatcaaaga gtgtga
    mRNA sequence
    • >TC012326 RA
      cgcttgaca actgtcaaa cgtgtcact tttcggctc 
      gtttaaaca ctattttct attaaataa acaattttc 
      gacgttcca ggaagcaca aaagcctaa aaactcact 
      gataagata agagcgaaa tgtgaattc ctctgttta 
      caggtttta gcacaatta tgttaacca ccaaaatgg 
      cactaggga aggaaactg agaggagtt taattcaga 
      aatatgtta caatatttc ttgtagtta gtggctatt 
      gggttgtct cgattttga ctgtgttta tcaacaaaa 
      ccctcctca gtgacatca gtttagacg ccccgatgt 
      tcattgcgt tgtatcaaa ccttcataa ccgcagcca 
      tttgtttcg ttaaaaaag gcttagctc gggtattcc 
      cccaacatt ttagtttcc ctgaaacga gcgtatggg 
      acgcacaaa caattaaaa caatcttgc ccgtgtctc 
      ttatgttta ctatgatga tcgcaatga acaatctct 
      gcttaaaat acgtctcag tcgctttct actacattg 
      gaaggtcct tgaccacga tttttaacg tagcgttca 
      cgttcgtaa tcttggggg agagaacgt ccaagcagt 
      gtttatttt tttgtggtg tcatcatat ttgggtttt 
      acttaggag tggaccagg aaagtctct ctgggagtt 
      tatcaataa gtggcacca tttttggtg ttttggctt 
      cgctgtctt tatcccttt attcaattt tcactaaaa 
      aagttctga caaaagttg ataaccaag tttgggcgt 
      tatcgtact acaacaata tttatgcaa ctattttat 
      tcattccct tgatgcttt ttaataacg aattctttg 
      tcttggccg attacagtg ggttatcac aaacatatt 
      tttggttta taatgacag ttgggggag tttgtggat 
      tcgcaattg gatttttta cctctttac aaatcaagt 
      atacgtcag ctttaacgc acaacatat caggcacag 
      ctaaggctt gtgcccaaa ctgtgttag ccacttact 
      ggtatcaag aagcaaagt cggcacttt ggtggtgtt 
      caaacttta ttgtcttgt tgggaagca tgggatatg 
      caagagtta aacaacttg atatggaga aaaaacata 
      aagagacca tgatgtatc aaagagtgt gataattgt 
      gcgactgtt tttaataaa aactgtgat ttgtataag 
      ataattaat caaattgat tatttactc taaaaatat 
      gtaaacaag ttgctggtt caaaaacac ataaaaata 
      ccaaagcgg tctataagt gtatactat gtacatagt 
      aatttaatt acgtgagtg tattttgta gcgataaga 
      aattgccgt tgtgtagag aggatacaa ttaaaactg 
      tattccagt tgaaatata aaattttat ggctaagtt 
      tttaaatat taatgccgt cctattagt ataaaattt 
      catgttcgg cgtgaatta acttgtagt acaaaaatc 
      attggtttt tagatgaca agtctggct ttttgtttg 
      ctatctatg aggattaaa ttttataag gtagatttt 
      ttttattaa gcaggtaac acttttata gttggtaac 
      tataaaaat taagcagtt ttgattgtc taggattta 
      atagccaat gacttctgc tgtgacttc ataacagtt 
      gtttctttg tacaaaaaa taatgtatt taattatgc 
      aagtgaaat aaagttttg cagttaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY