Status
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Gene information
General information
TC012311 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4008632..4010130
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012311
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05213

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Comment: animals cannibalized and not fully analyzable

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012311 RA
      MLNHLKRVI YLEKCPRGR FMSVKRKYP IDRDEAKSL 
      PVFDYGTSK KSYKRVFVW GNLMSGALG IPYLRRNEN 
      VMHRETVDI PKRLGFAEK FPVTTAACG FGFTAFGVD 
      TESEDKLYG TGLNTDSQI GRHEVRQGH PLEIIYFPQ 
      AIKLPLKNP TKTRIRKLA AGRAHLVVL TDEGLFLLG 
      NNAYGQCGR EIIQDENYF LSNYINHIP DIDSKKVTD 
      IECGQDHTL AVTDDGAVY SCGWGADGQ TGLGTFDNT 
      SAFTRITGE IESEKIVKI SSRSDFALA LNDRGEVFG 
      WGNAEYDQI PTINNEQQV SNPVHLKHL KVGKVVDVA 
      AGGSFCLAL NEHGEVFVW GYGLVLGGP KAEQTSRPR 
      KLPEVLFGR NDFQPNNRV VQVACGLSY AMAVTSLGD 
      VYAWGRNMR GCLGLGFAN DQNFPFKVA LGGYASKVF 
      CGYDHTVAL CKPFI
    • >TC012311 RB
      MAVTSLGDV YAWGRNMRG CLGLGGLRV EGVLRV
    CDS sequence
    • >TC012311 RA
      atgttaaat cacttgaaa cgggtgatt tatctcgaa 
      aaatgcccc cgtggacgc ttcatgtct gtaaaacgc 
      aaatacccc atcgatcgc gacgaggca aaatcactg 
      cctgtcttt gactacggt actagtaaa aagtcgtac 
      aaacgagtg tttgtttgg ggcaacctt atgtcgggg 
      gccttaggt attccgtat ttgagacgc aacgaaaat 
      gtcatgcat cgggagaca gtagacatc ccaaagcgg 
      ctaggtttt gctgaaaaa ttcccagtg actactgcc 
      gcttgtggc ttcggtttc accgcgttt ggtgttgat 
      accgaatct gaagataaa ttgtacgga acgggcctt 
      aataccgac tcacaaatt ggaagacat gaagtgcgt 
      caagggcac cctttggag attatttac ttcccgcag 
      gccattaaa ctaccttta aaaaaccca accaaaact 
      cggatacga aagttagcg gcggggcgt gcacatctc 
      gtggtgtta acagacgag ggactcttc ctcctaggc 
      aataacgcc tacggccag tgcgggcgc gaaatcatc 
      caagacgaa aattatttt ttaagtaat tacataaac 
      cacattcct gatatagac agtaagaaa gtaaccgac 
      attgaatgt ggtcaagac cacactttg gccgtaact 
      gacgacggg gctgtgtat tcctgcggg tggggggct 
      gacggccaa acgggcctc ggcacgttt gacaacaca 
      agtgccttc acgagaatc acgggcgag attgagtcc 
      gaaaaaata gtcaaaata tcgtcgcga tccgacttc 
      gcgttggcc cttaatgac aggggcgaa gtcttcggc 
      tggggcaac gctgagtac gatcagatc cccacaata 
      aacaacgag caacaagtg agcaaccca gtccacctc 
      aagcacctg aaagtgggc aaagtcgtg gatgtggca 
      gccgggggc tcattttgc ctcgctttg aacgaacac 
      ggggaggtt ttcgtgtgg ggctacggt ctagtcctc 
      ggggggccc aaggccgag caaacctcc cgcccgagg 
      aaactcccc gaggttttg ttcggcagg aacgacttt 
      caacctaac aacagggtg gtacaagtg gcttgtggg 
      ttgagctac gccatggct gtgacgagt ctgggggat 
      gtttacgcg tgggggcgc aacatgagg ggctgtctg 
      gggctgggg ttcgccaac gaccagaac ttcccgttt 
      aaagtggcg ctagggggc tacgcgtcg aaggtgttt 
      tgcgggtat gaccacacg gtggcactt tgtaaacct 
      tttatttaa
    • >TC012311 RB
      atggctgtg acgagtctg ggggatgtt tacgcgtgg 
      gggcgcaac atgaggggc tgtctgggg ctggggggg 
      ctacgcgtc gaaggtgtt ttgcgggta tga
    mRNA sequence
    • >TC012311 RA
      tagaatcca ccaacattg cgcgatctt cagctgact 
      tcacgcaag taaaaaaaa gtggggtta ataacaaca 
      aaaacatgt taaatcact tgaaacggg tgatttatc 
      tcgaaaaat gcccccgtg gacgcttca tgtctgtaa 
      aacgcaaat accccatcg atcgcgacg aggcaaaat 
      cactgcctg tctttgact acggtacta gtaaaaagt 
      cgtacaaac gagtgtttg tttggggca accttatgt 
      cgggggcct taggtattc cgtatttga gacgcaacg 
      aaaatgtca tgcatcggg agacagtag acatcccaa 
      agcggctag gttttgctg aaaaattcc cagtgacta 
      ctgccgctt gtggcttcg gtttcaccg cgtttggtg 
      ttgataccg aatctgaag ataaattgt acggaacgg 
      gccttaata ccgactcac aaattggaa gacatgaag 
      tgcgtcaag ggcaccctt tggagatta tttacttcc 
      cgcaggcca ttaaactac ctttaaaaa acccaacca 
      aaactcgga tacgaaagt tagcggcgg ggcgtgcac 
      atctcgtgg tgttaacag acgagggac tcttcctcc 
      taggcaata acgcctacg gccagtgcg ggcgcgaaa 
      tcatccaag acgaaaatt attttttaa gtaattaca 
      taaaccaca ttcctgata tagacagta agaaagtaa 
      ccgacattg aatgtggtc aagaccaca ctttggccg 
      taactgacg acggggctg tgtattcct gcgggtggg 
      gggctgacg gccaaacgg gcctcggca cgtttgaca 
      acacaagtg ccttcacga gaatcacgg gcgagattg 
      agtccgaaa aaatagtca aaatatcgt cgcgatccg 
      acttcgcgt tggccctta atgacaggg gcgaagtct 
      tcggctggg gcaacgctg agtacgatc agatcccca 
      caataaaca acgagcaac aagtgagca acccagtcc 
      acctcaagc acctgaaag tgggcaaag tcgtggatg 
      tggcagccg ggggctcat tttgcctcg ctttgaacg 
      aacacgggg aggttttcg tgtggggct acggtctag 
      tcctcgggg ggcccaagg ccgagcaaa cctcccgcc 
      cgaggaaac tccccgagg ttttgttcg gcaggaacg 
      actttcaac ctaacaaca gggtggtac aagtggctt 
      gtgggttga gctacgcca tggctgtga cgagtctgg 
      gggatgttt acgcgtggg ggcgcaaca tgaggggct 
      gtctggggc tggggttcg ccaacgacc agaacttcc 
      cgtttaaag tggcgctag ggggctacg cgtcgaagg 
      tgttttgcg ggtatgacc acacggtgg cactttgta 
      aacctttta tttaataaa tttaagagt aaaaaataa 
      tttcaattt tatgcc
    • >TC012311 RB
      tagaatcca ccaacattg cgcgatctt cagctgact 
      tcacgcaag taaaaaaaa gtggggtta ataacaaca 
      aaaacatgt taaatcact tgaaacggg tgatttatc 
      tcgaaaaat gcccccgtg gacgcttca tgtctgtaa 
      aacgcaaat accccatcg atcgcgacg aggcaaaat 
      cactgcctg tctttgact acggtacta gtaaaaagt 
      cgtacaaac gagtgtttg tttggggca accttatgt 
      cgggggcct taggtattc cgtatttga gacgcaacg 
      aaaatgtca tgcatcggg agacagtag acatcccaa 
      agcggctag gttttgctg aaaaattcc cagtgacta 
      ctgccgctt gtggcttcg gtttcaccg cgtttggtg 
      ttgataccg aatctgaag ataaattgt acggaacgg 
      gccttaata ccgactcac aaattggaa gacatgaag 
      tgcgtcaag ggcaccctt tggagatta tttacttcc 
      cgcaggcca ttaaactac ctttaaaaa acccaacca 
      aaactcgga tacgaaagt tagcggcgg ggcgtgcac 
      atctcgtgg tgttaacag acgagggac tcttcctcc 
      taggcaata acgcctacg gccagtgcg ggcgcgaaa 
      tcatccaag acgaaaatt attttttaa gtaattaca 
      taaaccaca ttcctgata tagacagta agaaagtaa 
      ccgacattg aatgtggtc aagaccaca ctttggccg 
      taactgacg acggggctg tgtattcct gcgggtggg 
      gggctgacg gccaaacgg gcctcggca cgtttgaca 
      acacaagtg ccttcacga gaatcacgg gcgagattg 
      agtccgaaa aaatagtca aaatatcgt cgcgatccg 
      acttcgcgt tggccctta atgacaggg gcgaagtct 
      tcggctggg gcaacgctg agtacgatc agatcccca 
      caataaaca acgagcaac aagtgagca acccagtcc 
      acctcaagc acctgaaag tgggcaaag tcgtggatg 
      tggcagccg ggggctcat tttgcctcg ctttgaacg 
      aacacgggg aggttttcg tgtggggct acggtctag 
      tcctcgggg ggcccaagg ccgagcaaa cctcccgcc 
      cgaggaaac tccccgagg ttttgttcg gcaggaacg 
      actttcaac ctaacaaca gggtggtac aagtggctt 
      gtgggttga gctacgcca tggctgtga cgagtctgg 
      gggatgttt acgcgtggg ggcgcaaca tgaggggct 
      gtctggggc tgggggggc tacgcgtcg aaggtgttt 
      tgcgggtat gaccacacg gtggcactt tgtaaacct 
      tttatttaa taa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY