Status
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Gene information
General information
TC012301 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3925602..3943000
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012301
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05211

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012301 RA
      MHCQYYLKR FPSIIIGHF WRILVDAPG ENPAACCWC 
      GNKLLYGLG GRHPEPYYG RLIGPLQEF AHGIKGTVY 
      AVDESTIFI KGFSYDGTG PDAFFWIGN SPRPSPEGI 
      IIPYPENYA GREPPVLRA YNNTNIILR LPMGKRIRD 
      IRWLSVWCR RFTVDFGEV FIPPNLEVP KPRVLPEFK 
      RLAHGLRSD NISILDAKT FYIPNLHYD GAGPDAYFW 
      VGNGSEPSP FGIKVPNEM GSLDPLRGY QGEDIEIQL 
      PGSITVYDI DWLAVWCVQ YRHNFGHVI IPKDLDVPP 
      ALGQTKIST STSKPSSPA NCQEFLDKR LQVRWEMRG 
      DYVQITLAA KIREDQYVA FGLSGAPTK SQMVGGDVT 
      VAFYDTNSR TFKAVDYYM STTAQCDGK SGVCPDERI 
      GGRNDVTLV SGDRKNGIT TITYMRPLQ TNEAVNDRP 
      IPSQGNISV IAAFGPLNS RKEANAHSI RDKTEGDYM 
      IDFSDVDHN MCSGDLDEL DDEDEIKPW PPAKITGES 
      TFTAKIGPT GGKRGYTAI TGQPSWGIA WYINDLLIP 
      EITVERGQT YTFIVEGGS DSSNPARYH PFYITDSVE 
      GGFGQKSEE EKRQQKVYA GVEYDREGY PYPTASGRY 
      CELAHKSID KSAESETFE EYLDTLRLE CDQGEPGYL 
      NWTVPMDAP DLLYYQCYT HNNLGWKIH VVNPGASQS 
      NGEAPSRSV SIIALAAIC LLTVSAITI HSRGRIF
    CDS sequence
    • >TC012301 RA
      atgcactgc caatattat ttgaagcgt ttccctagc 
      ataattatc ggtcatttc tggagaatt ttggtggat 
      gcgccgggc gaaaatccc gctgcctgt tgctggtgc 
      ggcaataaa ttgttgtat ggattaggt ggaaggcac 
      ccggaacct tactacggc cggctcatt ggacctttg 
      caggagttc gcccatgga ataaaggga actgtttat 
      gccgtggac gaaagcacg attttcata aagggattc 
      tcgtatgat ggaacagga cctgatgcg tttttctgg 
      attggaaac tcgccaaga ccgagtccg gagggcatt 
      attattccg tatccggag aattatgcg ggaagggag 
      cctccggta ttacgagct tacaacaac acgaacata 
      atcctaaga ctgccgatg ggtaaaaga attagagat 
      atccgatgg ctgtccgtg tggtgccgg cgctttacg 
      gtcgatttc ggcgaagtt ttcatccct ccaaactta 
      gaagtaccc aagcctcga gttcttccg gaattcaaa 
      cgactggct cacggcctg cgctcagac aatattagc 
      attttagat gccaaaact ttttacatt ccgaatttg 
      cactacgac ggggctgga cccgacgcc tacttctgg 
      gtgggcaac ggcagcgag ccctcgccc ttcggcatc 
      aaggtgccc aacgagatg ggctcgctg gacccgctg 
      cggggctac cagggcgag gacatcgag atccagctc 
      ccggggtcc atcacggtc tacgacatc gactggctg 
      gccgtctgg tgcgtgcaa taccggcac aacttcgga 
      cacgtcatc atccccaaa gacctggac gtgccgccg 
      gccctcggc cagaccaag atctcgacg agcacttca 
      aagccgtca agtcctgcg aattgtcaa gagttctta 
      gacaaacgg ctacaagta cggtgggaa atgagagga 
      gattacgtt caaatcaca ctggcagcg aaaatccgt 
      gaagatcaa tacgtcgcg tttggactc tcgggggct 
      cccaccaag tcgcaaatg gtcggcgga gacgtgacg 
      gtcgctttc tacgacacg aactctcga actttcaaa 
      gctgtggat tactacatg tccacaacg gcccaatgt 
      gacggcaag tctggcgtt tgtcccgac gaacgcatc 
      ggcggccga aacgacgtc accctggtc tcaggcgac 
      cgcaaaaac ggcatcacc accatcacc tacatgaga 
      ccgctccaa accaacgaa gccgtcaac gacaggccc 
      atacccagc caggggaac atcagcgtg atcgccgcc 
      ttcgggccc ctcaactcc cgcaaggag gccaacgct 
      cattccata agagacaag acggaaggc gattacatg 
      atcgatttc agcgacgtc gaccacaac atgtgcagc 
      ggcgatttg gacgagttg gacgacgag gacgagatc 
      aagccctgg ccgccggcc aagatcaca ggggaaagc 
      accttcacg gcgaaaatc gggccgacg ggcggcaag 
      cggggctac acagccatc acggggcag ccttcctgg 
      ggcatcgcc tggtacatc aatgacctc ctaatcccc 
      gaaataacc gtagaacgc ggccaaacc tacaccttc 
      atagtcgaa gggggtagt gatagcagc aatccggca 
      cgataccac cctttctac atcaccgac tcggtggag 
      ggcggtttt ggccaaaaa agcgaagag gagaagcgc 
      cagcaaaaa gtctatgct ggggtcgaa tacgaccgt 
      gaaggctac ccctatccc acagcctca gggaggtac 
      tgcgagctg gcacacaag agcatagac aagtcggcc 
      gaatcggag accttcgag gagtatttg gacacgctg 
      aggctggag tgcgaccag ggagagccg gggtatctg 
      aactggacg gtgccgatg gacgctccc gatctgctc 
      tactatcaa tgctatacg cataataat ctaggttgg 
      aagatacac gtggtcaat ccaggggcg tcgcagagc 
      aacggggag gcgccgtct aggagcgtc tcgataatt 
      gcacttgct gctatctgt ctactgacg gtttcagcc 
      atcacaata cattctcgt ggccgtatt ttctaa
    mRNA sequence
    • >TC012301 RA
      atcgtccaa acatgcact gccaatatt atttgaagc 
      gtttcccta gcataatta tcggtcatt tctggagaa 
      ttttggtgg atgcgccgg gcgaaaatc ccgctgcct 
      gttgctggt gcggcaata aattgttgt atggattag 
      gtggaaggc acccggaac cttactacg gccggctca 
      ttggacctt tgcaggagt tcgcccatg gaataaagg 
      gaactgttt atgccgtgg acgaaagca cgattttca 
      taaagggat tctcgtatg atggaacag gacctgatg 
      cgtttttct ggattggaa actcgccaa gaccgagtc 
      cggagggca ttattattc cgtatccgg agaattatg 
      cgggaaggg agcctccgg tattacgag cttacaaca 
      acacgaaca taatcctaa gactgccga tgggtaaaa 
      gaattagag atatccgat ggctgtccg tgtggtgcc 
      ggcgcttta cggtcgatt tcggcgaag ttttcatcc 
      ctccaaact tagaagtac ccaagcctc gagttcttc 
      cggaattca aacgactgg ctcacggcc tgcgctcag 
      acaatatta gcattttag atgccaaaa ctttttaca 
      ttccgaatt tgcactacg acggggctg gacccgacg 
      cctacttct gggtgggca acggcagcg agccctcgc 
      ccttcggca tcaaggtgc ccaacgaga tgggctcgc 
      tggacccgc tgcggggct accagggcg aggacatcg 
      agatccagc tcccggggt ccatcacgg tctacgaca 
      tcgactggc tggccgtct ggtgcgtgc aataccggc 
      acaacttcg gacacgtca tcatcccca aagacctgg 
      acgtgccgc cggccctcg gccagacca agatctcga 
      cgagcactt caaagccgt caagtcctg cgaattgtc 
      aagagttct tagacaaac ggctacaag tacggtggg 
      aaatgagag gagattacg ttcaaatca cactggcag 
      cgaaaatcc gtgaagatc aatacgtcg cgtttggac 
      tctcggggg ctcccacca agtcgcaaa tggtcggcg 
      gagacgtga cggtcgctt tctacgaca cgaactctc 
      gaactttca aagctgtgg attactaca tgtccacaa 
      cggcccaat gtgacggca agtctggcg tttgtcccg 
      acgaacgca tcggcggcc gaaacgacg tcaccctgg 
      tctcaggcg accgcaaaa acggcatca ccaccatca 
      cctacatga gaccgctcc aaaccaacg aagccgtca 
      acgacaggc ccataccca gccagggga acatcagcg 
      tgatcgccg ccttcgggc ccctcaact cccgcaagg 
      aggccaacg ctcattcca taagagaca agacggaag 
      gcgattaca tgatcgatt tcagcgacg tcgaccaca 
      acatgtgca gcggcgatt tggacgagt tggacgacg 
      aggacgaga tcaagccct ggccgccgg ccaagatca 
      caggggaaa gcaccttca cggcgaaaa tcgggccga 
      cgggcggca agcggggct acacagcca tcacggggc 
      agccttcct ggggcatcg cctggtaca tcaatgacc 
      tcctaatcc ccgaaataa ccgtagaac gcggccaaa 
      cctacacct tcatagtcg aagggggta gtgatagca 
      gcaatccgg cacgatacc accctttct acatcaccg 
      actcggtgg agggcggtt ttggccaaa aaagcgaag 
      aggagaagc gccagcaaa aagtctatg ctggggtcg 
      aatacgacc gtgaaggct acccctatc ccacagcct 
      cagggaggt actgcgagc tggcacaca agagcatag 
      acaagtcgg ccgaatcgg agaccttcg aggagtatt 
      tggacacgc tgaggctgg agtgcgacc agggagagc 
      cggggtatc tgaactgga cggtgccga tggacgctc 
      ccgatctgc tctactatc aatgctata cgcataata 
      atctaggtt ggaagatac acgtggtca atccagggg 
      cgtcgcaga gcaacgggg aggcgccgt ctaggagcg 
      tctcgataa ttgcacttg ctgctatct gtctactga 
      cggtttcag ccatcacaa tacattctc gtggccgta 
      ttttctaac gttttgatg aaaatcgcg acaataatt 
      ttatatgat gttgactga ttaagtatt tctgtgacg 
      agacaataa ctttatctt ctaataatg cgtccatat 
      cagctaaat atcgtggtg caattgaca gttccaaaa 
      acgtaactc ttctttttt gtttattaa aggttctac 
      aataacata tctggttct gacgccaaa ggacatgga 
      tttccgatt gtttgttgg gtttgttct cgttggtca 
      ttttgagtc tggaaagaa ttttgaagc tcacattct 
      acagagagt ttaaaaaat aaaaaaaga gatcaaaaa 
      aaaacgaag aaaatcaag tacctatta attttttat 
      tttaaaaca acgatgaaa tattgaaaa agaaaaaaa 
      tactcagac tcggagaaa tcagaggta ctggagaga 
      atcttttaa agctcgaaa cccttattt ctactctaa 
      agaaagttt tgagcaaga atattaaac attaatttt 
      aaactctta aggaaaaat aagtaaaaa aatcaaaag 
      agtatgcaa atacagtaa atgttgaaa tttgagaaa 
      taaagatca tagaacaaa cattcaaag acactggag 
      agaatctca atttcttta tttcttttc tacagagag 
      ttttataag aaaagactt tgaggtttg ttgaaatcc 
      aatttttct agaaaaaat tttgtctta aaaagctaa 
      ataaatttc gaataacaa actgcaaat gtgcaaaaa 
      aatataagg aaataataa aattaagac aaagtttaa 
      gggaaaaat aacgccatt ttagtgatt tttcagttt 
      tttgacaga attttgtgt aataatgtt gcgtttctg 
      gtttaaaaa cgtcttaaa gcaatctca aaaagataa 
      tactttttc tgaactttg aaacgtgcc tatacatga 
      tcaaaaaag ttgtcacaa caaataaaa ttcaataag 
      tcagtgtct tactacaat aaacaatac ggaaaaaca 
      aagacttaa ctttggaat aaaatttta aaattatta 
      cattaaaaa tcttatgaa acacctaac aatcttgaa 
      gtctgatct gctagaagg agtaaggat ttgggtgaa 
      atgtcttga gaaaaacgc aaattttaa gaaaaaatc 
      aagattaag gcaaacagc tggagattc cgtgacttt 
      tcgcttgca atttatcaa gtcctcaaa taacccttg 
      acagctgtt gaagtgtta aaactggga tacctgtat 
      gaagcatat cggaaaccc ttaccaaag aattattta 
      atttgtttg ctgatttcg aggcaaggg acacgcatg 
      aaaaaagga accaagaaa attatcgtg caaacatat 
      caaagactt tgtagaatc tttcctttg taggttttt 
      ctcctaatt ttgtgtttt ttgaatcag tggtgaaag 
      gattgtcaa gagaagtac atcactgtt tcgcgtatt 
      ttttgctag agtactagt agattgaaa tttttgttg 
      attatgtag aagagtggt tgcaaagtc ttacccaaa 
      tctaaaaat aattgcccg caattgtca tctcttggg 
      ctttttaat gaaataaaa ttgtgtctg cttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY