Status
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Gene information
General information
TC034243
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012292,TC012294,TC012293
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3831283..3839266
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034243
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05210

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034243 RA
      MHLNHENNK HFFTLKPAF LFQIFAAVS ASLVLISDG 
      MQYGWSSPV IPILESNNT PVKINADDS AWLETTFLL 
      SGPLALVVT PILVDRIGR HTTVLLISC ISIIGWVLI 
      GVATRIEML YVARFLLGA LSDIIYTTI PMYISEIAD 
      KEIRGLLNT ILYVMIYSG FIIIYAVAP SSRFYVPSI 
      VSAGISLLQ IILFWFMPE SPYFLAKKQ RYDSALKSL 
      KRLRVKDNC DTEFEEILN STQTSKTQS SIKEAIFKK 
      STRKAFACV TTLVIAQHF CGFSVIVMN LFTILEKAG 
      SIYLDSNTT QILFASLML AATCASCNV IDKFGRRVL 
      LIVSCTLTG INLLGLAVY FHAKFLGFS VAYFSWLPL 
      VCIMLYAVT FNVGLGLVP KILISELYS IRVKAIGMA 
      LAGGMFELF GSISIIFYK YTIRVCDVY VVFYIFTII 
      TFATVVFTV LAIPETKGK SLEEIQIIK TAMARFYTL 
      GNGHILQIL AALSASLLE ISDGMQYGW SSPVIPILE 
      SKNSPVKIT SEDSTWLET IFLLAGPLA LVITPVLVD 
      KIGRHKTIL IISCLSIIG WILIGVGTR VEILYLARF 
      LFGTVSDII FTTVAMYTS EIADKEIRG FLNGLMYVF 
      LYLGFILIY SLAPSTPFY VPSVVAAGI LLLQILTFI 
      PMPESPYFL MKKQRYESA MKSLKRLRS KTNCETEFE 
      KMKNCMEDS EHKSNIKEA IFKKSTLKA FICATILFV 
      AQHFCGLSV ILMNLYTIL EKAGSIYLD SNTTEILFA 
      LLMLVSMIV SCYVVDKFG RKNLLVVSS VFTGANLIG 
      LAVYFHLKC LGFNVDCLS WLPLIFVML YAVTFNIGL 
      GSVPKILVS ELYSIKVKA VGMSIGAGT YELAGSLSI 
      FISIPTTMR SKLTITRLY NSLKPQYPF QIWATLSAS 
      LLVISDGMQ FGWSSPVIP ILESKTSPI RITSEDSTW 
      LETIFLLAG PLILFVTPI LVDKIGRHK SILLVSFIS 
      ITGWILIGV ATRIEILYS ARFLFGMTT NIIFSAVPM 
      YTSEIAAKE IRGLLNGIV YVFFYLGFI LIYSIAPSS 
      PLYVPSIVA AGILLLQMF TFITMPESP YFLVKKQRY 
      ECAMKSLKR LRSKTNCEL EFEKIKNSL KDTDHRSNI 
      KDFSFIPTT MQTKITIIQ LYNSLKPQY PFQIWTILS 
      ASLLVISDG MQFGWSSPV IPILESKTS PIKITSDDA 
      TWLQTIFLL AGPLILFVT PISVDKIGR HKTILIVAC 
      ISILGWILI GVATRIEIL YCARFLFGM TTNIIFTTV 
      PMYTSEIAA KEIRGLLNG IVYVFFYLG FILIDSIAP 
      SSPFYVPSI VAAGILLLH ILTFITMPE SPYFLVRQR 
      RYECAMKCL QRLRSKNCD TELEEIRKC MAAAHRKSS 
      IREYISKKS TLKAFLCTT LLFVEQQTC GLAVILMNL 
      YTILEKAGS IYLDSNTTQ ILFAFLMLV SMFVACLMI 
      DKFGRKNLL VVSGVLTGA NLIGLAVYF HLKSLEFRV 
      DCLSWLPLM FVMFYAVTF NIGFGLVPK ILVSELFSI 
      RVKAVGMSI GAGTSELAA SLSIIFYKY VTQFYEMHF 
      VFYFFGASA FVTVLLTIF VIPETKGKS LEEIQGMLE 
      GATEHA
    CDS sequence
    • >TC034243 RA
      atgcatctg aaccatgaa aataacaaa cattttttc 
      accttaaaa cccgcgttt ctttttcaa attttcgca 
      gctgtaagt gcttcgctt gtcctcatt tcggatggc 
      atgcaatac ggttggtcg tcccccgta atacccatc 
      ttagaatcc aacaatact cccgtcaaa atcaatgca 
      gatgattca gcttggcta gaaacaacg ttcctatta 
      tcaggacct ctggcctta gttgtgaca ccaatttta 
      gtggacaga ataggtcgc cacaccacc gttttatta 
      atctcctgc atttctata atcggatgg gttctcatc 
      ggagttgct actcgcata gaaatgtta tacgtcgcc 
      agatttcta cttggagcc ctctctgac atcatttac 
      acaacaata cccatgtac atatcggaa atcgccgat 
      aaagaaatc aggggcctc ctaaacaca atcctgtac 
      gtcatgatt tactccgga tttatcata atctacgct 
      gtagcgccc tcgagccgc ttctacgtc ccttcgata 
      gtgtctgca ggaatctca cttttgcaa ataattttg 
      ttctggttc atgccagag tctccgtat ttcttggcg 
      aaaaaacaa cgctacgac agtgcactc aaatcgctg 
      aaaagactg cgcgtgaaa gacaactgt gataccgag 
      ttcgaagaa atcctcaac tccacacaa acaagcaaa 
      acccagagc agcataaaa gaagccatt ttcaagaaa 
      agtacgcgc aaagccttc gcctgtgtg accacgctg 
      gtcatagcg cagcatttt tgcggcttt agtgttatt 
      gtaatgaac ttgttcaca attctcgaa aaagctggc 
      tcaatatat ctggactct aacacgaca caaatatta 
      ttcgcctcg ctaatgttg gcagcaacg tgtgcttcc 
      tgcaacgtg attgataaa tttggccga agggttttg 
      ttaattgtg tcctgtacg ttaacaggg ataaatctg 
      ctaggatta gctgtttat ttccatgct aaatttttg 
      ggattcagt gttgcttat ttcagttgg ctgcctttg 
      gtttgtatc atgttgtat gcagtcact tttaatgtt 
      ggactggga ttggtgccg aaaatttta atttcggag 
      ttgtactct atcagagtt aaagcaatt ggcatggcg 
      cttgcgggg ggcatgttt gaattgttc ggctcaatt 
      tctatcatt ttttacaag tatacgata agagtttgc 
      gatgtgtac gtagttttt tacattttt acaataatt 
      actttcgct actgttgtc tttactgtt ctggcaatc 
      ccagaaact aagggtaaa tcgttggaa gaaattcaa 
      attataaaa acggcaatg gcacgcttt tacactttg 
      ggcaatgga cacatttta caaattttg gcagcccta 
      agtgcttct cttttggaa atttcggat ggaatgcaa 
      tacggttgg tcctctcct gtgattccc atcttagaa 
      tcaaagaat tctccagtc aaaatcact tcagaggac 
      tccacatgg ttggaaact attttcctg ctagcagga 
      cctctggcg ttagtcata acaccggtt ctcgtggac 
      aaaatcggc cgccacaag acgatttta atcatctct 
      tgcctttcg ataatcgga tggattctc atcggagtc 
      gggactcgt gtagaaatt ttataccta gccagattt 
      ttatttggt accgtatca gatattatt ttcacaaca 
      gtggcaatg tatacatcg gagatcgcc gacaaagaa 
      attcgcggc ttcttaaac ggtcttatg tacgtcttc 
      ttatacttg ggcttcatt ctgatatac tctctagcg 
      ccatcaact ccattttac gttccttcg gtagtggca 
      gcaggaatt ttactgtta caaatactc acatttatt 
      ccgatgcct gaatcgccc tattttttg atgaaaaaa 
      caacgttac gaaagtgca atgaaatcg ttgaagcga 
      ctgcgatcg aaaaccaac tgtgagacg gaatttgaa 
      aaaatgaaa aactgcatg gaagacagt gaacacaaa 
      agtaacata aaagaagcc attttcaag aaaagcact 
      ctcaaggca tttatttgt gcaactata ctgtttgtg 
      gcgcaacat ttctgcggc ttaagtgtt attttaatg 
      aacttgtac acgattctc gaaaaagct ggctccata 
      tatctcgat tcaaacacg acagaaata ttgtttgct 
      ttgttgatg ttggtttcg atgattgtg tcgtgctac 
      gtggttgat aaatttggg cgaaaaaat ctgttggtg 
      gtctcaagt gtctttaca ggagcaaac ttaattggg 
      ttggccgtt tattttcac ctgaagtgt ttaggattc 
      aatgttgat tgtcttagt tggttacct ttaatattt 
      gttatgttg tatgccgtt acgtttaat attgggttg 
      ggatcagta cccaaaatt ttggtttct gaattatat 
      tccattaaa gttaaagca gtcggaatg tcaatcggt 
      gccggaact tacgaactt gctggatct ctttctatt 
      tttatttca attcctact acaatgcga tctaaatta 
      acaataaca cgtctttac aactcgcta aaacctcaa 
      taccctttt caaatttgg gcaacctta agtgcttcg 
      cttcttgta atctccgat ggaatgcaa ttcggttgg 
      tcctctcct gtgattccc attttagaa tcaaaaact 
      tctcctatt aggatcacg tcagaagac tctacttgg 
      ttggaaacg atattcctt ttagcagga cctctgata 
      ttatttgta acaccgatt ttagttgac aaaatcggc 
      cgtcacaaa agtatttta ttagtctcc ttcatttca 
      ataaccgga tggattctt atcggagtt gcgactcgt 
      atagaaatc ctgtacagt gcaagattt ctgtttggg 
      atgacaaca aatataatt ttttcggca gtaccaatg 
      tacacatca gaaatcgcc gccaaggaa attcgcggt 
      ttactaaac ggaatcgtt tacgttttc ttttacctt 
      ggattcatc ttgatatac tccatagct ccatcaagc 
      ccactatac gtaccttca atagtggct gcgggaatt 
      ttactatta caaatgttc acatttatc accatgcct 
      gagtcccca tattttttg gtgaaaaaa caacgttat 
      gagtgtgca atgaaatct ttgaagagg ctgcgctcg 
      aaaaccaac tgtgagttg gaatttgag aaaatcaaa 
      aacagcttg aaagataca gaccacaga agtaacata 
      aaagatttt tccttcatt ccgactaca atgcaaact 
      aaaataaca ataatacaa ctttacaat tcgctgaaa 
      ccccaatac ccctttcaa atttggaca atcctaagt 
      gcctcgctt cttgtgatc tcagatgga atgcaattc 
      ggttggtca tctcctgtg attcccatt ttggaatca 
      aaaacctct cctattaaa atcacgtca gacgacgct 
      acttggttg caaacgata ttcctatta gcaggacct 
      ctgatatta tttgtaaca ccgatttca gttgacaaa 
      atcggccgc cacaaaacc attttaata gtcgcctgc 
      atttctata ctcggatgg attctcata ggagttgcg 
      actcgtata gaaattctg tactgtgca agatttctg 
      ttcgggatg acaacaaat ataattttt acgacagtg 
      ccaatgtac acatcagaa atcgccgcc aaagaaatt 
      cgcggttta ctaaacgga atcgtttac gttttcttc 
      taccttgga ttcatcttg atagactcc atagctcca 
      tcaagccca ttctacgta ccttcaata gtggctgca 
      ggaatttta cttttacat atactcaca tttatcacc 
      atgcctgag tccccatat tttttggta agacaacga 
      cgttacgaa tgtgcaatg aaatgtttg cagagattg 
      cgatcgaaa aactgtgat actgaactt gaagaaatc 
      agaaaatgc atggcagct gcgcaccgc aaaagtagc 
      atacgagaa tacatttca aaaaaaagt actctcaaa 
      gcatttctt tgtacaacc ttactgttt gtggagcag 
      caaacttgc ggtctagct gttatttta atgaacttg 
      tacacaatt cttgaaaaa gctggttcg atatatctt 
      gattcaaac acgacgcaa atattattt gccttctta 
      atgttggtt tcaatgttt gttgcgtgc ctcatgatt 
      gataaattt ggacgaaaa aatctgcta gttgtctcg 
      ggtgtctta acaggagca aatttaatt gggttggcc 
      gtttatttt cacctgaag agtttagaa ttcagggtt 
      gattgtcta agttggttg cctttaatg tttgttatg 
      ttttatgcc gttacgttt aatattggg ttcggatta 
      gtacccaaa attttagtc tctgaacta ttttccatt 
      agagttaaa gcagtcgga atgtctatt ggtgccggc 
      acatccgaa cttgcggca tctctttcc attatattt 
      tataaatat gtcacccaa ttttatgaa atgcacttc 
      gttttttac ttttttgga gcaagtgct tttgtaact 
      gttttactt acaatcttc gtaattccg gagacaaaa 
      ggcaaatct ttggaagaa atacaagga atgctcgaa 
      ggagcaacc gaacatgct taa
    mRNA sequence
    • >TC034243 RA
      cagtgacgc aaaatcgaa ggtaaacca gaaataaat 
      aaacttgaa aatacgatg agactgaaa agtacagct 
      actagttgt ttaaaataa ttaatttaa aaaagacag 
      aattattac atgagtgtc agcaaagct gaatattaa 
      aattatttc tcctaattc tgatacaaa ttggtaacg 
      cttttttct cttttagtt ttcgaaaca atgcatctg 
      aaccatgaa aataacaaa cattttttc accttaaaa 
      cccgcgttt ctttttcaa attttcgca gctgtaagt 
      gcttcgctt gtcctcatt tcggatggc atgcaatac 
      ggttggtcg tcccccgta atacccatc ttagaatcc 
      aacaatact cccgtcaaa atcaatgca gatgattca 
      gcttggcta gaaacaacg ttcctatta tcaggacct 
      ctggcctta gttgtgaca ccaatttta gtggacaga 
      ataggtcgc cacaccacc gttttatta atctcctgc 
      atttctata atcggatgg gttctcatc ggagttgct 
      actcgcata gaaatgtta tacgtcgcc agatttcta 
      cttggagcc ctctctgac atcatttac acaacaata 
      cccatgtac atatcggaa atcgccgat aaagaaatc 
      aggggcctc ctaaacaca atcctgtac gtcatgatt 
      tactccgga tttatcata atctacgct gtagcgccc 
      tcgagccgc ttctacgtc ccttcgata gtgtctgca 
      ggaatctca cttttgcaa ataattttg ttctggttc 
      atgccagag tctccgtat ttcttggcg aaaaaacaa 
      cgctacgac agtgcactc aaatcgctg aaaagactg 
      cgcgtgaaa gacaactgt gataccgag ttcgaagaa 
      atcctcaac tccacacaa acaagcaaa acccagagc 
      agcataaaa gaagccatt ttcaagaaa agtacgcgc 
      aaagccttc gcctgtgtg accacgctg gtcatagcg 
      cagcatttt tgcggcttt agtgttatt gtaatgaac 
      ttgttcaca attctcgaa aaagctggc tcaatatat 
      ctggactct aacacgaca caaatatta ttcgcctcg 
      ctaatgttg gcagcaacg tgtgcttcc tgcaacgtg 
      attgataaa tttggccga agggttttg ttaattgtg 
      tcctgtacg ttaacaggg ataaatctg ctaggatta 
      gctgtttat ttccatgct aaatttttg ggattcagt 
      gttgcttat ttcagttgg ctgcctttg gtttgtatc 
      atgttgtat gcagtcact tttaatgtt ggactggga 
      ttggtgccg aaaatttta atttcggag ttgtactct 
      atcagagtt aaagcaatt ggcatggcg cttgcgggg 
      ggcatgttt gaattgttc ggctcaatt tctatcatt 
      ttttacaag tatacgata agagtttgc gatgtgtac 
      gtagttttt tacattttt acaataatt actttcgct 
      actgttgtc tttactgtt ctggcaatc ccagaaact 
      aagggtaaa tcgttggaa gaaattcaa attataaaa 
      acggcaatg gcacgcttt tacactttg ggcaatgga 
      cacatttta caaattttg gcagcccta agtgcttct 
      cttttggaa atttcggat ggaatgcaa tacggttgg 
      tcctctcct gtgattccc atcttagaa tcaaagaat 
      tctccagtc aaaatcact tcagaggac tccacatgg 
      ttggaaact attttcctg ctagcagga cctctggcg 
      ttagtcata acaccggtt ctcgtggac aaaatcggc 
      cgccacaag acgatttta atcatctct tgcctttcg 
      ataatcgga tggattctc atcggagtc gggactcgt 
      gtagaaatt ttataccta gccagattt ttatttggt 
      accgtatca gatattatt ttcacaaca gtggcaatg 
      tatacatcg gagatcgcc gacaaagaa attcgcggc 
      ttcttaaac ggtcttatg tacgtcttc ttatacttg 
      ggcttcatt ctgatatac tctctagcg ccatcaact 
      ccattttac gttccttcg gtagtggca gcaggaatt 
      ttactgtta caaatactc acatttatt ccgatgcct 
      gaatcgccc tattttttg atgaaaaaa caacgttac 
      gaaagtgca atgaaatcg ttgaagcga ctgcgatcg 
      aaaaccaac tgtgagacg gaatttgaa aaaatgaaa 
      aactgcatg gaagacagt gaacacaaa agtaacata 
      aaagaagcc attttcaag aaaagcact ctcaaggca 
      tttatttgt gcaactata ctgtttgtg gcgcaacat 
      ttctgcggc ttaagtgtt attttaatg aacttgtac 
      acgattctc gaaaaagct ggctccata tatctcgat 
      tcaaacacg acagaaata ttgtttgct ttgttgatg 
      ttggtttcg atgattgtg tcgtgctac gtggttgat 
      aaatttggg cgaaaaaat ctgttggtg gtctcaagt 
      gtctttaca ggagcaaac ttaattggg ttggccgtt 
      tattttcac ctgaagtgt ttaggattc aatgttgat 
      tgtcttagt tggttacct ttaatattt gttatgttg 
      tatgccgtt acgtttaat attgggttg ggatcagta 
      cccaaaatt ttggtttct gaattatat tccattaaa 
      gttaaagca gtcggaatg tcaatcggt gccggaact 
      tacgaactt gctggatct ctttctatt tttatttca 
      attcctact acaatgcga tctaaatta acaataaca 
      cgtctttac aactcgcta aaacctcaa taccctttt 
      caaatttgg gcaacctta agtgcttcg cttcttgta 
      atctccgat ggaatgcaa ttcggttgg tcctctcct 
      gtgattccc attttagaa tcaaaaact tctcctatt 
      aggatcacg tcagaagac tctacttgg ttggaaacg 
      atattcctt ttagcagga cctctgata ttatttgta 
      acaccgatt ttagttgac aaaatcggc cgtcacaaa 
      agtatttta ttagtctcc ttcatttca ataaccgga 
      tggattctt atcggagtt gcgactcgt atagaaatc 
      ctgtacagt gcaagattt ctgtttggg atgacaaca 
      aatataatt ttttcggca gtaccaatg tacacatca 
      gaaatcgcc gccaaggaa attcgcggt ttactaaac 
      ggaatcgtt tacgttttc ttttacctt ggattcatc 
      ttgatatac tccatagct ccatcaagc ccactatac 
      gtaccttca atagtggct gcgggaatt ttactatta 
      caaatgttc acatttatc accatgcct gagtcccca 
      tattttttg gtgaaaaaa caacgttat gagtgtgca 
      atgaaatct ttgaagagg ctgcgctcg aaaaccaac 
      tgtgagttg gaatttgag aaaatcaaa aacagcttg 
      aaagataca gaccacaga agtaacata aaagatttt 
      tccttcatt ccgactaca atgcaaact aaaataaca 
      ataatacaa ctttacaat tcgctgaaa ccccaatac 
      ccctttcaa atttggaca atcctaagt gcctcgctt 
      cttgtgatc tcagatgga atgcaattc ggttggtca 
      tctcctgtg attcccatt ttggaatca aaaacctct 
      cctattaaa atcacgtca gacgacgct acttggttg 
      caaacgata ttcctatta gcaggacct ctgatatta 
      tttgtaaca ccgatttca gttgacaaa atcggccgc 
      cacaaaacc attttaata gtcgcctgc atttctata 
      ctcggatgg attctcata ggagttgcg actcgtata 
      gaaattctg tactgtgca agatttctg ttcgggatg 
      acaacaaat ataattttt acgacagtg ccaatgtac 
      acatcagaa atcgccgcc aaagaaatt cgcggttta 
      ctaaacgga atcgtttac gttttcttc taccttgga 
      ttcatcttg atagactcc atagctcca tcaagccca 
      ttctacgta ccttcaata gtggctgca ggaatttta 
      cttttacat atactcaca tttatcacc atgcctgag 
      tccccatat tttttggta agacaacga cgttacgaa 
      tgtgcaatg aaatgtttg cagagattg cgatcgaaa 
      aactgtgat actgaactt gaagaaatc agaaaatgc 
      atggcagct gcgcaccgc aaaagtagc atacgagaa 
      tacatttca aaaaaaagt actctcaaa gcatttctt 
      tgtacaacc ttactgttt gtggagcag caaacttgc 
      ggtctagct gttatttta atgaacttg tacacaatt 
      cttgaaaaa gctggttcg atatatctt gattcaaac 
      acgacgcaa atattattt gccttctta atgttggtt 
      tcaatgttt gttgcgtgc ctcatgatt gataaattt 
      ggacgaaaa aatctgcta gttgtctcg ggtgtctta 
      acaggagca aatttaatt gggttggcc gtttatttt 
      cacctgaag agtttagaa ttcagggtt gattgtcta 
      agttggttg cctttaatg tttgttatg ttttatgcc 
      gttacgttt aatattggg ttcggatta gtacccaaa 
      attttagtc tctgaacta ttttccatt agagttaaa 
      gcagtcgga atgtctatt ggtgccggc acatccgaa 
      cttgcggca tctctttcc attatattt tataaatat 
      gtcacccaa ttttatgaa atgcacttc gttttttac 
      ttttttgga gcaagtgct tttgtaact gttttactt 
      acaatcttc gtaattccg gagacaaaa ggcaaatct 
      ttggaagaa atacaagga atgctcgaa ggagcaacc 
      gaacatgct taaaagaat caaactgtt ttatttata 
      ttatatgta ttaaaaatt gtattacat t
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