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Gene information
General information
TC012285 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3786153..3790012
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012285
    iBeetle screen iB_05208

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012285 RA
      MFIRNLFLF CILLCASES YKILAIFPT PSHSHHTLA 
      SKLVKELAK RKHEVTFVS PFPQKETVE NLKEISVEE 
      IIPIFNQQK KHLFKLNEL TLFENVIFV NNIGLLVTE 
      ELLRNEKIQ KLLESNEHF DLIILPQFI NEGLLAIGH 
      HFKAPVVLL SSMPLFSWS SFLLGHPTS SSYVPNIQT 
      HYTGHMNFW QRLCNSIYD AYSILYHQW VVLPKHRQL 
      VKKYVPGQP DLYDFLNNA SLVLVNSHV SSYDATIQV 
      PNVVEIGGF HLEEAKKLP EDLQKYLDG SKEGVVIFS 
      MGTLMKSSS MPKEKLQAL LQAFSKLKQ QVLWKWDSE 
      EMEGKPGNV KLVKWLPQS DVLAHPNVK VFITHGGLL 
      STMESIYHG VPTLGIPVF TDQKANIEF AVEAGSGIA 
      VPYPELSEE KLSQALDQI INNASYKTN ALKRSKIMK 
      DRPIHPLDY AIYWIEYVA RHEGAPHLR YPGMGLAWY 
      QRYLIDVAF FVGCECYKF LALFQTSTK SHYILASKL 
      FKEIAKKGH EVTFITPFE EQTPTKNLN VVPVKTLQT 
      FFCTRRNSM FEFESANPL EKAKFLFEI SYNFTEHLI 
      QSTEVQTLL KSDEKFDGV IIYQYLNEA LLGVAHHFK 
      APVILFSSM PLYAPESFL LSHPNPPSY VPNILVEYT 
      GRMNFWQRL RNTFYDTSM IAYYLWNYL PKHRELVRK 
      YVPGGPDLY DFVNNASLI LINSHVSAN EAVPLVPNA 
      VEIGGYHIE EPKALPQDL QKFLDDSKN GVILFSMGS 
      IVQSTHFPE EKRRELFKT FAKLKENVL WKWEGDDFP 
      GLPKNVKVM KWIPQSDVL AHPNVRAFI SHGGLLSTM 
      ESVYHAVPI VGIPVMADQ KMNIELAVS YGYAVAVPY 
      PELREETLT KALDKVLND PTYRNNIKK RSLIMKDRP 
      IKPLDNALY WIEYVIRHE GAPHLRYPG MDLTWYQRN 
      LLDVAGFVL VVIFSLVFV IVKICKLLF REKSKAKVV 
      SKKKKNN
    CDS sequence
    • >TC012285 RA
      atgttcata cggaatttg tttctattt tgcattctt 
      ttgtgtgct tccgagagt tacaaaatc ttagcaatc 
      ttccctaca ccgtcccat agtcaccac accttagcc 
      tccaaactc gtaaaggaa ttggcgaaa aggaaacat 
      gaagtgact tttgttagt ccattccct caaaaggaa 
      accgtggaa aatttgaag gaaattagt gtagaggaa 
      ataatacca atatttaac caacaaaag aagcattta 
      tttaaactc aacgagctg acacttttc gagaatgtg 
      atctttgtg aacaacatt gggcttctt gtaacggaa 
      gagttactc cgaaatgag aagatacaa aaattgtta 
      gagtcaaac gaacatttc gatctaata attctgccc 
      cagtttata aatgagggc ttgttggcc atcggccac 
      cacttcaaa gcaccggtg gtacttctg tcatccatg 
      ccccttttc tcatggagc agttttctc ctcggacac 
      ccaacttcc tcatcttat gttccaaac atccagaca 
      cattacaca gggcatatg aacttttgg caacgactt 
      tgcaacagc atttacgac gcttattcc atcctttac 
      caccaatgg gtcgtacta ccaaaacac agacagctg 
      gtaaagaaa tatgtacca ggacagccc gacttgtac 
      gattttctc aataacgcc agtttagtg ctagttaac 
      tcgcacgtg agttcgtat gatgccaca attcaagta 
      ccaaatgtc gtggaaatt ggtggtttt cacctcgaa 
      gaggcgaaa aaactaccg gaggatctc caaaaatat 
      ttggatggt tccaaggag ggagttgtg atattttca 
      atggggaca ctaatgaaa agttcgagc atgccaaag 
      gaaaaattg caagcactg ctccaggca ttttccaaa 
      ctgaagcaa caagttttg tggaaatgg gacagtgaa 
      gaaatggaa ggaaagccg ggaaatgtc aaattggtc 
      aagtggttg ccacagtcc gatgtttta gcccatcca 
      aacgtaaaa gtgtttatc acccatggt ggcctttta 
      agtaccatg gaaagcata taccacggg gtaccaaca 
      ttaggaatt ccggttttt accgaccag aaagcaaat 
      attgaattt gcagtggag gcagggtct ggcattgcc 
      gttccctat ccagaatta tcggaagaa aaattatct 
      caagctctg gaccaaatt ataaataat gcaagctac 
      aagacaaac gccttgaag cggtcgaaa ataatgaag 
      gaccgaccc atacaccca ttggattat gccatttac 
      tggatagag tatgtcgcc cgacatgaa ggggcacca 
      catcttagg taccctgga atgggcttg gcttggtac 
      caacgatat ttaatcgat gtcgcattt ttcgtgggt 
      tgtgagtgt tacaaattt ttagctctc ttccaaacg 
      tccactaaa agtcactac atactcgcc tcaaaactg 
      ttcaaagag attgcaaag aagggacac gaagttacg 
      tttattacc ccgtttgag gagcaaacg cctacaaaa 
      aatctaaat gttgtgccg gtcaaaacc ttgcaaaca 
      tttttctgc acacgtcga aactcgatg tttgaattc 
      gagagtgca aatccgctg gagaaagcc aaatttctt 
      ttcgagata agttacaac ttcaccgag cacttaatc 
      cagagcacg gaggtccag actttgcta aaatcggac 
      gaaaaattc gacggcgtg attatttat caatacctg 
      aatgaggcc cttctcgga gtcgctcac catttcaaa 
      gcgccggtg attctattc tcttcaatg ccactctac 
      gcccctgaa agtttcctt ctgtcacac cccaatcct 
      ccctcttat gtccccaac atcctggtt gagtacacc 
      ggtcgtatg aatttctgg caacggctg aggaacact 
      ttctacgac acgtcgatg atcgcgtat tacctgtgg 
      aattacctg ccgaaacac cgggaactc gtccgaaaa 
      tatgtgcca ggagggcca gacctctac gatttcgtt 
      aacaacgct agtcttatt ttaatcaat tcgcacgtc 
      agtgccaac gaggccgtt cctctggtc ccaaacgcg 
      gtcgaaatc gggggctac cacatcgaa gaacccaaa 
      gcgctacct caggacttg caaaaattc ttagacgac 
      tccaaaaac ggtgtcata cttttttcg atgggctcg 
      attgtgcaa agtacgcac tttcccgaa gagaaacgc 
      agagagctt tttaaaact ttcgccaaa ttgaaggaa 
      aatgttttg tggaaatgg gaaggagac gattttccc 
      ggtttgcca aaaaatgtt aaagtgatg aagtggata 
      ccacaatcg gacgtcctc gcccaccct aacgtccga 
      gcttttatc agccatgga ggtttgttg agtaccatg 
      gaaagtgtg taccatgct gtcccaatt gtgggaatt 
      cccgtaatg gcagaccag aaaatgaac atagagctg 
      gccgtttcg tacggttat gctgtcgca gtcccttat 
      ccggaatta agagaggaa actttgacg aaagctctg 
      gataaagta ttaaacgat ccgacgtac cgcaataac 
      attaaaaaa agatcactt ataatgaaa gatcggccg 
      attaaacct ttggataac gctctttac tggattgaa 
      tacgttatc cggcacgag ggcgcgcca catctcaga 
      taccccgga atggattta acgtggtat caaagaaat 
      ctgctcgat gttgcggga tttgttctt gttgtgata 
      ttttctcta gtctttgtt attgtaaag atatgcaaa 
      ttattgttt agggaaaaa tccaaagct aaagtcgtt 
      agcaaaaag aagaaaaat aactaa
    mRNA sequence
    • >TC012285 RA
      cttttcaat agaacacgt ggccttgat gccacttca 
      taaatgcag tcgatgatg taatcagct gcagtctat 
      tattaacta tgtaaacat gttcatacg gaatttgtt 
      tctattttg cattctttt gtgtgcttc cgagagtta 
      caaaatctt agcaatctt ccctacacc gtcccatag 
      tcaccacac cttagcctc caaactcgt aaaggaatt 
      ggcgaaaag gaaacatga agtgacttt tgttagtcc 
      attccctca aaaggaaac cgtggaaaa tttgaagga 
      aattagtgt agaggaaat aataccaat atttaacca 
      acaaaagaa gcatttatt taaactcaa cgagctgac 
      acttttcga gaatgtgat ctttgtgaa caacattgg 
      gcttcttgt aacggaaga gttactccg aaatgagaa 
      gatacaaaa attgttaga gtcaaacga acatttcga 
      tctaataat tctgcccca gtttataaa tgagggctt 
      gttggccat cggccacca cttcaaagc accggtggt 
      acttctgtc atccatgcc ccttttctc atggagcag 
      ttttctcct cggacaccc aacttcctc atcttatgt 
      tccaaacat ccagacaca ttacacagg gcatatgaa 
      cttttggca acgactttg caacagcat ttacgacgc 
      ttattccat cctttacca ccaatgggt cgtactacc 
      aaaacacag acagctggt aaagaaata tgtaccagg 
      acagcccga cttgtacga ttttctcaa taacgccag 
      tttagtgct agttaactc gcacgtgag ttcgtatga 
      tgccacaat tcaagtacc aaatgtcgt ggaaattgg 
      tggttttca cctcgaaga ggcgaaaaa actaccgga 
      ggatctcca aaaatattt ggatggttc caaggaggg 
      agttgtgat attttcaat ggggacact aatgaaaag 
      ttcgagcat gccaaagga aaaattgca agcactgct 
      ccaggcatt ttccaaact gaagcaaca agttttgtg 
      gaaatggga cagtgaaga aatggaagg aaagccggg 
      aaatgtcaa attggtcaa gtggttgcc acagtccga 
      tgttttagc ccatccaaa cgtaaaagt gtttatcac 
      ccatggtgg ccttttaag taccatgga aagcatata 
      ccacggggt accaacatt aggaattcc ggtttttac 
      cgaccagaa agcaaatat tgaatttgc agtggaggc 
      agggtctgg cattgccgt tccctatcc agaattatc 
      ggaagaaaa attatctca agctctgga ccaaattat 
      aaataatgc aagctacaa gacaaacgc cttgaagcg 
      gtcgaaaat aatgaagga ccgacccat acacccatt 
      ggattatgc catttactg gatagagta tgtcgcccg 
      acatgaagg ggcaccaca tcttaggta ccctggaat 
      gggcttggc ttggtacca acgatattt aatcgatgt 
      cgcattttt cgtgggttg tgagtgtta caaattttt 
      agctctctt ccaaacgtc cactaaaag tcactacat 
      actcgcctc aaaactgtt caaagagat tgcaaagaa 
      gggacacga agttacgtt tattacccc gtttgagga 
      gcaaacgcc tacaaaaaa tctaaatgt tgtgccggt 
      caaaacctt gcaaacatt tttctgcac acgtcgaaa 
      ctcgatgtt tgaattcga gagtgcaaa tccgctgga 
      gaaagccaa atttctttt cgagataag ttacaactt 
      caccgagca cttaatcca gagcacgga ggtccagac 
      tttgctaaa atcggacga aaaattcga cggcgtgat 
      tatttatca atacctgaa tgaggccct tctcggagt 
      cgctcacca tttcaaagc gccggtgat tctattctc 
      ttcaatgcc actctacgc ccctgaaag tttccttct 
      gtcacaccc caatcctcc ctcttatgt ccccaacat 
      cctggttga gtacaccgg tcgtatgaa tttctggca 
      acggctgag gaacacttt ctacgacac gtcgatgat 
      cgcgtatta cctgtggaa ttacctgcc gaaacaccg 
      ggaactcgt ccgaaaata tgtgccagg agggccaga 
      cctctacga tttcgttaa caacgctag tcttatttt 
      aatcaattc gcacgtcag tgccaacga ggccgttcc 
      tctggtccc aaacgcggt cgaaatcgg gggctacca 
      catcgaaga acccaaagc gctacctca ggacttgca 
      aaaattctt agacgactc caaaaacgg tgtcatact 
      tttttcgat gggctcgat tgtgcaaag tacgcactt 
      tcccgaaga gaaacgcag agagctttt taaaacttt 
      cgccaaatt gaaggaaaa tgttttgtg gaaatggga 
      aggagacga ttttcccgg tttgccaaa aaatgttaa 
      agtgatgaa gtggatacc acaatcgga cgtcctcgc 
      ccaccctaa cgtccgagc ttttatcag ccatggagg 
      tttgttgag taccatgga aagtgtgta ccatgctgt 
      cccaattgt gggaattcc cgtaatggc agaccagaa 
      aatgaacat agagctggc cgtttcgta cggttatgc 
      tgtcgcagt cccttatcc ggaattaag agaggaaac 
      tttgacgaa agctctgga taaagtatt aaacgatcc 
      gacgtaccg caataacat taaaaaaag atcacttat 
      aatgaaaga tcggccgat taaaccttt ggataacgc 
      tctttactg gattgaata cgttatccg gcacgaggg 
      cgcgccaca tctcagata ccccggaat ggatttaac 
      gtggtatca aagaaatct gctcgatgt tgcgggatt 
      tgttcttgt tgtgatatt ttctctagt ctttgttat 
      tgtaaagat atgcaaatt attgtttag ggaaaaatc 
      caaagctaa agtcgttag caaaaagaa gaaaaataa 
      ctaattaca aatatcaat aaagaattg gaaacgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY