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Gene information
General information
TC012282 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3768513..3774182
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012282
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05207

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012282 RA
      MVYQMPAHS HYSLGLRIA KELAERGHQ VTIINPYPQ 
      KTPIKNFRD VSVEDHIEF IQAFKKSLF SRNSMSLFG 
      NINFLIGLG AELTNRTLG HVNVQKLLR SGETFDLLI 
      AEYFFNDAI IGLGHYFKI PVVLTAPNA VSGVNNNLF 
      VNPTPASYV PHVLSPYNK HMNFWQRLH NWYIGLLSN 
      LMKEFILMP RHCAIFKQH VSQDVELDE TLGNVSLVF 
      TNSHASVTD AVPHQPNMV EIGGYHVDP PKKLPGDLQ 
      QFLDGAKEG AILFSMGSN LKSRDLKPE VRSGILAAF 
      SKIKQKVLW KFEVEFDDC PENVKIVNW VPQQDALAH 
      PNIVAFISH GGLLSTIET VYHGVPIIG IPVFGDQPS 
      NIAAAVANG YAVSIDLFE LNEAKLSWA LDEILNNPK 
      YRENAKQRS KIMQDQPLK PIDAAIYWV EHVIRHQGA 
      PHLRSAALD LAWYQREMM DIFAFLGLV AATHFSLGF 
      RLAKELADR GHQMTVINP YPQKTPIKN YKDVSVEEN 
      VEFIEELKK QLFKFNTLG PYDNLHAIY GMGIDLTEN 
      TFQNKRVQD LLKSGETFD LVIMEHFLN DAEVGIAHH 
      FKAPFVLIS PFGLSRLNN HVLGNPLPS SYIPNLIGT 
      FSKHMTFWE RLQNFLLNI LTDLVRELS FMPRQRQMF 
      KKYIKTDLE LDDVLYNAS LMMTNSHVS VNDAVPRVP 
      GVIEIGGFH VNPPKKLPE DLQKFLDES KDGVVLFSM 
      GSNLKSKDL QPEVRDAIL QSFSKIKQK VLWKFETDL 
      PNAPKNVKI MKWLPQQDI LAHPNVVAF ITHGGLLST 
      LETVTRGVP IIGIPVFGD QKANIAAAV TDGYGVSVP 
      LPELSEEKL SWALNEILN NPKYRQNVK QRSKLMNDQ 
      PLKPLDSAV YWVEHVLRH GGAPHLRSA ALDLKWYQR 
      EMVDIFLFL ALVAIITLV VICKIIKIC IVWSAQSAK 
      ILVNAPMPA YSHFSLSFR LAKELADRG HQVTVINPY 
      PQKTPIKNY RDISVEEHV EYIEELKKN LFGMNNMGF 
      VANINFLLT MCSDLTERT LINKNVQQL LNSGETFDL 
      VITAHFLNE AHFGLAHHF KAPLVVLSP GAANSFTNH 
      FVANPSPSS YVPNVMAGF TNHMSFWQR TTNLIANAV 
      FDGTREAIM MPIQRKIFK KYIKSDVEL DDVLFNVSL 
      ILTNSHVSV QDAIPHVPN MIEIGGFHV KPPKKLPDD 
      LQKFLDESK EGVILFSMG SNLKSKDLK QDVRDGILK 
      AFSKIKQKV LWKFETELE GAPKNVKIM KWLPQQDIL 
      AHQNVVAFI SHGGLLSTI EAVYHGVPI IGIPVFGDQ 
      ESNIAAAAS KGYAVPVPL KELSEEKLS WALNEILNN 
      PKYRENIKQ RSKLMQDQP LKPIDSAIY WIEHVIRHK 
      GAPHLKSAG LDLKWYQRE MIDIFLFLT LVTVVLFVI 
      FYLIIKKIL GLCCGKKPV KVSKTKKNK
    CDS sequence
    • >TC012282 RA
      atggtatac caaatgcct gcacacagt cactactcg 
      ttagggctg cgaattgca aaagaatta gctgagaga 
      ggacaccaa gtcactata attaaccca tacccccaa 
      aaaacgcca attaaaaat tttagagat gtgtcagtt 
      gaggaccat atagaattt atccaagcg tttaagaaa 
      agtctattt tcgagaaac tcgatgagc ctctttgga 
      aatataaat ttcctgata ggacttggt gccgagtta 
      actaaccga acacttgga cacgtaaac gtccaaaaa 
      ttgctacgt tcgggcgag acgtttgat ctcctaatt 
      gcggaatat tttttcaac gacgctatt atcggcctc 
      ggccattat ttcaaaatc ccggtcgtt ctaacggcc 
      cctaatgcc gtcagcggt gttaacaac aatctcttc 
      gttaacccg acccccgct tcgtatgtg cctcacgtc 
      ctgagccct tataacaaa cacatgaac ttctggcaa 
      cgactccac aactggtac atcggactt ttgagcaat 
      ctcatgaag gaatttatc ttaatgcct cgccattgc 
      gctattttc aaacaacac gtgtctcaa gacgttgaa 
      ctggacgag acgctgggc aatgtgagt ttagtgttc 
      actaattca cacgctagc gtgaccgat gccgttccc 
      caccaacca aacatggtt gaaattggg ggatatcac 
      gtggaccca cctaaaaaa ttacccggg gatttacaa 
      caatttctc gatggggcc aaggagggc gcaatcttg 
      ttctcgatg gggtcaaat ctcaaaagc cgggatttg 
      aaaccggag gtgaggtcc gggattttg gccgccttt 
      tcgaaaatc aagcaaaaa gttttgtgg aagtttgag 
      gtcgagttc gatgattgt ccagagaat gttaaaatt 
      gtgaactgg gtgccacag caagacgcg cttgcacat 
      ccgaatatt gtggcgttt atttcgcac ggtggtctg 
      ctaagtacc atcgaaact gtctaccat ggagtccca 
      attatcgga attccagtt tttggggac caaccaagt 
      aacattgcg gcggccgta gccaacggt tatgctgtc 
      agtatcgac ttgttcgaa ctaaacgag gctaaatta 
      tcatgggct ttggacgaa atattgaac aacccaaaa 
      tatcgggaa aatgccaaa cagcggtcg aaaataatg 
      caagatcag cccttgaaa cccatcgat gctgcaatt 
      tattgggtg gaacacgtg ataaggcat caaggagcc 
      ccccattta cggtcagct gcccttgat ttagcatgg 
      taccaaagg gagatgatg gatattttt gcgtttctt 
      ggccttgtt gcagcaacc cacttttct ctgggcttc 
      cgtctggcg aaagaacta gctgatcga gggcatcaa 
      atgactgtg ataaatccc tacccccaa aaaacaccc 
      atcaaaaac tacaaagat gtttcagtt gaagaaaac 
      gtggaattt atcgaagaa ttgaagaaa caattattt 
      aagtttaac accttggga ccgtacgat aacttgcat 
      gccatctat ggtatgggt attgatttg accgaaaac 
      accttccag aacaaacgc gtccaagat ctcttaaaa 
      tcaggcgaa actttcgat ctcgtcata atggagcat 
      ttcctgaac gacgctgag gtcgggatc gcccaccac 
      ttcaaagcc cctttcgtc ctaatctca ccctttggc 
      ctcagcaga ctaaacaac cacgtctta ggaaacccg 
      ttaccatcc tcttacatc ccaaatctc ataggaacg 
      ttctccaaa cacatgact ttctgggaa cgcctccaa 
      aatttcctc ctcaacatt ttaactgat ctagtcaga 
      gagctgagt ttcatgcca cgacagcgc caaatgttc 
      aaaaagtac ataaaaacg gatctcgaa ctggacgac 
      gttttgtac aacgcgagt ctgatgatg accaactcg 
      cacgttagc gtgaacgac gctgttcca cgcgtccct 
      ggcgtgatc gaaattggg ggctttcat gtaaacccc 
      cccaaaaaa ttacccgag gatttgcaa aaatttctc 
      gacgagtcg aaggacgga gtcgtcctg ttttccatg 
      ggctccaat ttgaaaagc aaggacttg caaccggaa 
      gttcgtgac gctattctg caatcgttt tcgaaaatc 
      aagcaaaaa gtcttgtgg aaatttgaa acggacctt 
      cccaacgcc cccaagaac gtgaaaatc atgaaatgg 
      ttgccgcag caggacatt ttagcccac ccaaatgtt 
      gtggcgttt ataacgcac gggggtttg ctaagtaca 
      ctggagacg gtgactcga ggggtcccc ataatcggg 
      attcctgtt tttggggac cagaaggcc aacattgcg 
      gctgccgtg actgatggt tatggtgtc agtgttcct 
      ttgccggaa ctatccgag gaaaaacta tcatgggct 
      ttgaacgaa atcctgaac aatcctaag taccgccaa 
      aatgtgaaa caacggtcg aaactgatg aacgaccaa 
      ccactgaaa ccgcttgat tcggcggtt tattgggtc 
      gaacacgtg ctcaggcat ggaggtgcc ccacatctt 
      aggtctgca gcacttgat ctcaaatgg taccagcgc 
      gaaatggtc gatatattc ctgttcctg gcgcttgtc 
      gcaattatc acacttgtt gtaatttgc aaaatcatc 
      aaaatttgt attgtatgg agtgcccaa agtgccaaa 
      attttggtc aatgctcca atgccggcc tatagtcat 
      ttttctttg agctttcgc ctggctaag gaattggcg 
      gatcgtggc caccaggtc actgttata aacccctac 
      ccccagaaa accccaatt aaaaattat agagacata 
      tctgtggag gaacatgtg gaatatatc gaagaattg 
      aagaaaaat ttattcgga atgaataac atgggattc 
      gtcgcgaat ataaatttt cttctaacg atgtgttcg 
      gacttaacc gagcgcacg ttaattaac aaaaatgtc 
      caacaactt ttaaactcc ggtgaaact ttcgatttg 
      gtcataacg gcgcatttc ctaaacgaa gcccacttc 
      ggcctagcc catcacttc aaagcacct cttgttgtg 
      ctatcccct ggggctgct aattcgttc actaatcac 
      ttcgtcgct aatccatcc ccttcctct tacgtcccc 
      aacgtcatg gctggcttc accaaccac atgagtttc 
      tggcaacgg accacgaac ttgatcgca aacgccgta 
      tttgacggc acgagggag gccatcatg atgccaatc 
      cagcgaaaa attttcaaa aaatacata aaatccgac 
      gtggagctg gacgacgtg ttgttcaat gtcagcctt 
      attttgacc aattctcac gttagcgtg caagatgcc 
      atcccccac gtgcccaac atgatcgaa attgggggc 
      tttcacgtc aaacccccg aaaaaacta cccgatgat 
      ttgcaaaaa ttcctcgac gagtccaag gaaggcgtt 
      atcttattc tcgatgggt tcaaacttg aaaagcaag 
      gacttgaaa caagacgtt agggacggc attctgaag 
      gcgttttcg aagattaag caaaaggtg ttgtggaaa 
      tttgagacc gaattggaa ggcgccccc aaaaatgtg 
      aaaataatg aaatggctg ccgcagcaa gatattcta 
      gcgcaccag aacgttgtc gcgtttatt tcacacggt 
      ggtctattg agtaccatc gaagcggtc taccatggt 
      gtcccaatc attggtatt cccgttttc ggggaccag 
      gagtccaac attgctgct gctgcaagc aagggttat 
      gctgttcct gtgccgttg aaagaacta tcagaagag 
      aagctttcc tgggctttg aacgaaatc ctcaataat 
      ccaaagtac cgggaaaat atcaaacag cgttcgaaa 
      ttaatgcaa gatcagcca ctgaaaccc attgattcc 
      gcaatctac tggattgaa cacgtcata cgtcataaa 
      ggagcacca cacctgaag tccgcgggc cttgatctc 
      aagtggtac cagagggaa atgatcgac atattcctg 
      ttcttgact ctagtcact gtagtattg tttgttatt 
      ttttatcta attattaag aaaattctt ggactgtgt 
      tgcggaaaa aaaccggtc aaagtgtcc aagacgaag 
      aaaaacaaa taa
    mRNA sequence
    • >TC012282 RA
      tattgataa aaataaata cccacagtc aatttttgt 
      gaggcaatg aagcacttt atcacattt ctagtcatt 
      tttgtaggt ttattatgc aaaatccaa tgtgcaaaa 
      atactaatg gtataccaa atgcctgca cacagtcac 
      tactcgtta gggctgcga attgcaaaa gaattagct 
      gagagagga caccaagtc actataatt aacccatac 
      ccccaaaaa acgccaatt aaaaatttt agagatgtg 
      tcagttgag gaccatata gaatttatc caagcgttt 
      aagaaaagt ctattttcg agaaactcg atgagcctc 
      tttggaaat ataaatttc ctgatagga cttggtgcc 
      gagttaact aaccgaaca cttggacac gtaaacgtc 
      caaaaattg ctacgttcg ggcgagacg tttgatctc 
      ctaattgcg gaatatttt ttcaacgac gctattatc 
      ggcctcggc cattatttc aaaatcccg gtcgttcta 
      acggcccct aatgccgtc agcggtgtt aacaacaat 
      ctcttcgtt aacccgacc cccgcttcg tatgtgcct 
      cacgtcctg agcccttat aacaaacac atgaacttc 
      tggcaacga ctccacaac tggtacatc ggacttttg 
      agcaatctc atgaaggaa tttatctta atgcctcgc 
      cattgcgct attttcaaa caacacgtg tctcaagac 
      gttgaactg gacgagacg ctgggcaat gtgagttta 
      gtgttcact aattcacac gctagcgtg accgatgcc 
      gttccccac caaccaaac atggttgaa attggggga 
      tatcacgtg gacccacct aaaaaatta cccggggat 
      ttacaacaa tttctcgat ggggccaag gagggcgca 
      atcttgttc tcgatgggg tcaaatctc aaaagccgg 
      gatttgaaa ccggaggtg aggtccggg attttggcc 
      gccttttcg aaaatcaag caaaaagtt ttgtggaag 
      tttgaggtc gagttcgat gattgtcca gagaatgtt 
      aaaattgtg aactgggtg ccacagcaa gacgcgctt 
      gcacatccg aatattgtg gcgtttatt tcgcacggt 
      ggtctgcta agtaccatc gaaactgtc taccatgga 
      gtcccaatt atcggaatt ccagttttt ggggaccaa 
      ccaagtaac attgcggcg gccgtagcc aacggttat 
      gctgtcagt atcgacttg ttcgaacta aacgaggct 
      aaattatca tgggctttg gacgaaata ttgaacaac 
      ccaaaatat cgggaaaat gccaaacag cggtcgaaa 
      ataatgcaa gatcagccc ttgaaaccc atcgatgct 
      gcaatttat tgggtggaa cacgtgata aggcatcaa 
      ggagccccc catttacgg tcagctgcc cttgattta 
      gcatggtac caaagggag atgatggat atttttgcg 
      tttcttggc cttgttgca gcaacccac ttttctctg 
      ggcttccgt ctggcgaaa gaactagct gatcgaggg 
      catcaaatg actgtgata aatccctac ccccaaaaa 
      acacccatc aaaaactac aaagatgtt tcagttgaa 
      gaaaacgtg gaatttatc gaagaattg aagaaacaa 
      ttatttaag tttaacacc ttgggaccg tacgataac 
      ttgcatgcc atctatggt atgggtatt gatttgacc 
      gaaaacacc ttccagaac aaacgcgtc caagatctc 
      ttaaaatca ggcgaaact ttcgatctc gtcataatg 
      gagcatttc ctgaacgac gctgaggtc gggatcgcc 
      caccacttc aaagcccct ttcgtccta atctcaccc 
      tttggcctc agcagacta aacaaccac gtcttagga 
      aacccgtta ccatcctct tacatccca aatctcata 
      ggaacgttc tccaaacac atgactttc tgggaacgc 
      ctccaaaat ttcctcctc aacatttta actgatcta 
      gtcagagag ctgagtttc atgccacga cagcgccaa 
      atgttcaaa aagtacata aaaacggat ctcgaactg 
      gacgacgtt ttgtacaac gcgagtctg atgatgacc 
      aactcgcac gttagcgtg aacgacgct gttccacgc 
      gtccctggc gtgatcgaa attgggggc tttcatgta 
      aaccccccc aaaaaatta cccgaggat ttgcaaaaa 
      tttctcgac gagtcgaag gacggagtc gtcctgttt 
      tccatgggc tccaatttg aaaagcaag gacttgcaa 
      ccggaagtt cgtgacgct attctgcaa tcgttttcg 
      aaaatcaag caaaaagtc ttgtggaaa tttgaaacg 
      gaccttccc aacgccccc aagaacgtg aaaatcatg 
      aaatggttg ccgcagcag gacatttta gcccaccca 
      aatgttgtg gcgtttata acgcacggg ggtttgcta 
      agtacactg gagacggtg actcgaggg gtccccata 
      atcgggatt cctgttttt ggggaccag aaggccaac 
      attgcggct gccgtgact gatggttat ggtgtcagt 
      gttcctttg ccggaacta tccgaggaa aaactatca 
      tgggctttg aacgaaatc ctgaacaat cctaagtac 
      cgccaaaat gtgaaacaa cggtcgaaa ctgatgaac 
      gaccaacca ctgaaaccg cttgattcg gcggtttat 
      tgggtcgaa cacgtgctc aggcatgga ggtgcccca 
      catcttagg tctgcagca cttgatctc aaatggtac 
      cagcgcgaa atggtcgat atattcctg ttcctggcg 
      cttgtcgca attatcaca cttgttgta atttgcaaa 
      atcatcaaa atttgtatt gtatggagt gcccaaagt 
      gccaaaatt ttggtcaat gctccaatg ccggcctat 
      agtcatttt tctttgagc tttcgcctg gctaaggaa 
      ttggcggat cgtggccac caggtcact gttataaac 
      ccctacccc cagaaaacc ccaattaaa aattataga 
      gacatatct gtggaggaa catgtggaa tatatcgaa 
      gaattgaag aaaaattta ttcggaatg aataacatg 
      ggattcgtc gcgaatata aattttctt ctaacgatg 
      tgttcggac ttaaccgag cgcacgtta attaacaaa 
      aatgtccaa caactttta aactccggt gaaactttc 
      gatttggtc ataacggcg catttccta aacgaagcc 
      cacttcggc ctagcccat cacttcaaa gcacctctt 
      gttgtgcta tcccctggg gctgctaat tcgttcact 
      aatcacttc gtcgctaat ccatcccct tcctcttac 
      gtccccaac gtcatggct ggcttcacc aaccacatg 
      agtttctgg caacggacc acgaacttg atcgcaaac 
      gccgtattt gacggcacg agggaggcc atcatgatg 
      ccaatccag cgaaaaatt ttcaaaaaa tacataaaa 
      tccgacgtg gagctggac gacgtgttg ttcaatgtc 
      agccttatt ttgaccaat tctcacgtt agcgtgcaa 
      gatgccatc ccccacgtg cccaacatg atcgaaatt 
      gggggcttt cacgtcaaa cccccgaaa aaactaccc 
      gatgatttg caaaaattc ctcgacgag tccaaggaa 
      ggcgttatc ttattctcg atgggttca aacttgaaa 
      agcaaggac ttgaaacaa gacgttagg gacggcatt 
      ctgaaggcg ttttcgaag attaagcaa aaggtgttg 
      tggaaattt gagaccgaa ttggaaggc gcccccaaa 
      aatgtgaaa ataatgaaa tggctgccg cagcaagat 
      attctagcg caccagaac gttgtcgcg tttatttca 
      cacggtggt ctattgagt accatcgaa gcggtctac 
      catggtgtc ccaatcatt ggtattccc gttttcggg 
      gaccaggag tccaacatt gctgctgct gcaagcaag 
      ggttatgct gttcctgtg ccgttgaaa gaactatca 
      gaagagaag ctttcctgg gctttgaac gaaatcctc 
      aataatcca aagtaccgg gaaaatatc aaacagcgt 
      tcgaaatta atgcaagat cagccactg aaacccatt 
      gattccgca atctactgg attgaacac gtcatacgt 
      cataaagga gcaccacac ctgaagtcc gcgggcctt 
      gatctcaag tggtaccag agggaaatg atcgacata 
      ttcctgttc ttgactcta gtcactgta gtattgttt 
      gttattttt tatctaatt attaagaaa attcttgga 
      ctgtgttgc ggaaaaaaa ccggtcaaa gtgtccaag 
      acgaagaaa aacaaataa aagattcca cctttgatt 
      tatgtgttt cattctttc ccaatgaac aataaaaat 
      gcacagcaa tc
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