Status
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Gene information
General information
TC032528
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012267,TC012268
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 293343..317593
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032528
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05203

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_01958

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032528 RA
      MFIKPQEVL LASPFWETE ETSIYFLLQ HRKGHGNSK 
      GLSSLLVGT IDSIFDTKP APYRILHQT PSSEVYYLI 
      ACSMTRQEI LKDWDWLFN NVSATLHCF DTEDEITDF 
      VTCKIESVI ATRQEVDVE DEDSKSFKV TSDKFIRLF 
      GLSKDEKLV NYYSCCYWK GKLPRQGWM YLSVHYCCF 
      YAFILGLDT KVCIRWSDV IELSKKNSI VFPDSIKIV 
      TREKEYHFS MFLTKNETF KLMEQLVDL AMKRLIDDK 
      RSFSEDKEL LHKLSARVS GDSTRKRSL HKDSFLTVQ 
      QQCPARSEI SPCSCTLKK NGLDILCEF TDQQHITSA 
      MDKLKGREF VIFYLKLRH NNLRKLTGY IFLGLDIHH 
      LTIHNSSLS TMEEMSLSS IGKMLTQLD VSQNSLTAV 
      PSSAFASLH QLLILNMNH NKISVVHSK AFQGLDTLE 
      ILTLYENKI TTIEDGAFV GLEKKLKRL NLGGNSLTE 
      VPQKALSIL DTLKKLEMQ ENRLTEIKE GDFEGLKNL 
      DSLGLAHNK LRQVPSRVF SHLTVLNSL ELDGNNIDT 
      IDPEAFAGL EENLQYLRL GDNNIHTIP TEALKRLHR 
      LRHLDLRSN NISYIAEDA FIGFGDSIT FLNLQKNFI 
      RTLTGLTFE NLNSLETLN LQNNKLMHI GEDVMEPIL 
      DTLRVVDIM DNPLLCNCE LQWYKNWLR SLKDKDDEM 
      MQKKRTVCM MQQEHREYN LQNLPLGKM NCVIKSYDN 
      SPSGSNRIG YGVASMVMS LWACLVRFV
    CDS sequence
    • >TC032528 RA
      atgttcatt aaaccccaa gaagtccta ctagccagc 
      cccttttgg gaaacggaa gaaacgtca atttacttt 
      ttattgcaa catcgaaag ggacatggc aattccaag 
      gggctgtcg tcacttctt gtgggcact atcgatagt 
      atttttgac actaaaccg gcaccctac aggattttg 
      caccaaaca ccgtcgtcc gaagtctat tatttaatt 
      gcatgttct atgacgaga caagaaatc ctaaaagac 
      tgggattgg ctctttaac aatgtttca gctactttg 
      cactgcttt gatactgag gatgagatc accgacttc 
      gttacgtgc aaaattgag tcggttata gccacaaga 
      caagaagtt gatgttgag gatgaggac tcaaaatcg 
      ttcaaagtc acatccgac aaatttatt cggcttttc 
      ggtctctcg aaagacgaa aaacttgtc aactactat 
      tcctgttgc tactggaag ggtaaactg ccgcgccaa 
      ggatggatg tatttgtct gtacactat tgttgcttc 
      tatgctttc atcttaggt ctagacact aaagtttgt 
      ataagatgg tcagacgtg atcgaattg agcaaaaaa 
      aatagtatc gtttttccc gattctatc aaaatcgta 
      acgagggaa aaagaatat cacttcagt atgtttctc 
      accaaaaat gagactttc aaactgatg gaacagttg 
      gtcgatctg gccatgaag agattaatc gacgacaag 
      aggagcttt agcgaagat aaagagctc ttacacaag 
      ctgagtgct cgggtgtct ggggattca actcgcaag 
      cgctcgttg cataaagac agctttcta actgtgcag 
      cagcagtgt ccagcgcgt tcggagatc tcaccatgc 
      tcgtgtacg ttaaagaaa aacggttta gacatcctc 
      tgcgagttc accgaccag caacacata actagtgcc 
      atggataag ttaaaagga agagaattt gtaatattc 
      tatttgaag cttcgacac aacaacttg cgcaagttg 
      accggttac atattcctc ggtttggat atccatcac 
      ttgactatc cacaatagc agtttatca actatggaa 
      gagatgtct ttgagttcg attggcaaa atgctgacc 
      caactcgat gtgtcccaa aatagccta acggccgtt 
      ccatcttcg gcctttgcg agcctgcac cagctcctt 
      atcttgaac atgaaccac aacaaaatc agtgtcgtg 
      cattcaaag gcttttcag ggactagac actctcgaa 
      atactcact ctgtatgaa aacaagatc accaccatc 
      gaagacgga gctttcgtc ggactagaa aagaagtta 
      aaaaggttg aatttgggt ggcaacagc ttaacggaa 
      gttccgcaa aaagccctc tcaattctc gacacgctg 
      aaaaagctg gagatgcaa gaaaatcgc ctgacggaa 
      attaaagaa ggcgatttt gaaggtctg aaaaatctt 
      gattcgtta ggattggcc cacaataag cttaggcaa 
      gttccatcg agagttttc tcgcatctc acagttttg 
      aattctttg gaacttgat gggaacaat attgacacg 
      atcgatcct gaagctttt gccggactt gaagaaaat 
      cttcaatat ttgcgatta ggagacaac aacattcac 
      actattccc actgaagcc ctaaaacgt ctccacagg 
      ctccgtcat ttggacctc cgatcaaac aatatcagt 
      tatattgca gaagatgcc tttattggc ttcggagat 
      tcgatcacc tttcttaat cttcaaaaa aatttcatt 
      cgcaccttg acaggacta actttcgag aacctcaac 
      tctcttgaa accttaaac ttgcaaaac aacaaacta 
      atgcacatc ggggaggac gtgatggag cccattttg 
      gacactttg cgcgtggtc gatattatg gataatccc 
      ttactgtgc aactgtgag cttcaatgg tacaagaac 
      tggctaagg agtttgaag gataaagac gatgaaatg 
      atgcagaag aagcgaacc gtgtgcatg atgcaacaa 
      gaacaccgg gagtataac ttgcaaaat ttgcctttg 
      ggaaaaatg aattgcgtt ataaagtcg tatgataat 
      tcaccgagc ggttctaac cggatcggt tacggtgtt 
      gcgtcgatg gtcatgagc ttgtgggct tgtttagtg 
      agattcgtt taa
    mRNA sequence
    • >TC032528 RA
      actgttgac atttaacag ctgtcacag tcaagtcct 
      gtctgtttt tgtaacaca agttcagtg cgttttatc 
      tatgattta gttcttatc aaagttaaa tacagtgag 
      agattctat tcaaaattg aaatgcgtt cgtgtgaac 
      tgaaacacg tcgtcatcg gcaaaaatc ctctcacat 
      tgtgacact ttcctaaga tgtttagtt tctcaaccc 
      aacaccatg ttcattaaa ccccaagaa gtcctacta 
      gccagcccc ttttgggaa acggaagaa acgtcaatt 
      tacttttta ttgcaacat cgaaaggga catggcaat 
      tccaagggg ctgtcgtca cttcttgtg ggcactatc 
      gatagtatt tttgacact aaaccggca ccctacagg 
      attttgcac caaacaccg tcgtccgaa gtctattat 
      ttaattgca tgttctatg acgagacaa gaaatccta 
      aaagactgg gattggctc tttaacaat gtttcagct 
      actttgcac tgctttgat actgaggat gagatcacc 
      gacttcgtt acgtgcaaa attgagtcg gttatagcc 
      acaagacaa gaagttgat gttgaggat gaggactca 
      aaatcgttc aaagtcaca tccgacaaa tttattcgg 
      cttttcggt ctctcgaaa gacgaaaaa cttgtcaac 
      tactattcc tgttgctac tggaagggt aaactgccg 
      cgccaagga tggatgtat ttgtctgta cactattgt 
      tgcttctat gctttcatc ttaggtcta gacactaaa 
      gtttgtata agatggtca gacgtgatc gaattgagc 
      aaaaaaaat agtatcgtt tttcccgat tctatcaaa 
      atcgtaacg agggaaaaa gaatatcac ttcagtatg 
      tttctcacc aaaaatgag actttcaaa ctgatggaa 
      cagttggtc gatctggcc atgaagaga ttaatcgac 
      gacaagagg agctttagc gaagataaa gagctctta 
      cacaagctg agtgctcgg gtgtctggg gattcaact 
      cgcaagcgc tcgttgcat aaagacagc tttctaact 
      gtgcagcag cagtgtcca gcgcgttcg gagatctca 
      ccatgctcg tgtacgtta aagaaaaac ggtttagac 
      atcctctgc gagttcacc gaccagcaa cacataact 
      agtgccatg gataagtta aaaggaaga gaatttgta 
      atattctat ttgaagctt cgacacaac aacttgcgc 
      aagttgacc ggttacata ttcctcggt ttggatatc 
      catcacttg actatccac aatagcagt ttatcaact 
      atggaagag atgtctttg agttcgatt ggcaaaatg 
      ctgacccaa ctcgatgtg tcccaaaat agcctaacg 
      gccgttcca tcttcggcc tttgcgagc ctgcaccag 
      ctccttatc ttgaacatg aaccacaac aaaatcagt 
      gtcgtgcat tcaaaggct tttcaggga ctagacact 
      ctcgaaata ctcactctg tatgaaaac aagatcacc 
      accatcgaa gacggagct ttcgtcgga ctagaaaag 
      aagttaaaa aggttgaat ttgggtggc aacagctta 
      acggaagtt ccgcaaaaa gccctctca attctcgac 
      acgctgaaa aagctggag atgcaagaa aatcgcctg 
      acggaaatt aaagaaggc gattttgaa ggtctgaaa 
      aatcttgat tcgttagga ttggcccac aataagctt 
      aggcaagtt ccatcgaga gttttctcg catctcaca 
      gttttgaat tctttggaa cttgatggg aacaatatt 
      gacacgatc gatcctgaa gcttttgcc ggacttgaa 
      gaaaatctt caatatttg cgattagga gacaacaac 
      attcacact attcccact gaagcccta aaacgtctc 
      cacaggctc cgtcatttg gacctccga tcaaacaat 
      atcagttat attgcagaa gatgccttt attggcttc 
      ggagattcg atcaccttt cttaatctt caaaaaaat 
      ttcattcgc accttgaca ggactaact ttcgagaac 
      ctcaactct cttgaaacc ttaaacttg caaaacaac 
      aaactaatg cacatcggg gaggacgtg atggagccc 
      attttggac actttgcgc gtggtcgat attatggat 
      aatccctta ctgtgcaac tgtgagctt caatggtac 
      aagaactgg ctaaggagt ttgaaggat aaagacgat 
      gaaatgatg cagaagaag cgaaccgtg tgcatgatg 
      caacaagaa caccgggag tataacttg caaaatttg 
      cctttggga aaaatgaat tgcgttata aagtcgtat 
      gataattca ccgagcggt tctaaccgg atcggttac 
      ggtgttgcg tcgatggtc atgagcttg tgggcttgt 
      ttagtgaga ttcgtttaa tattaatta tacttagca 
      atgtgtttt atttgtaat tatgcaagt tagcgttag 
      ctagaattt taacgttga tattttata tgtaaaatt 
      agagtttaa cctgttttg catcaatca atatgaaat 
      tagagcatg tacgttgct ttactgata gacgagcgt 
      atttaagct tcttgttat caatttctt atagatttg 
      tggagccgg tcggagaca cttcgattt tcattgaag 
      ttgctttat gaacatcat gagtgattg tgtctcttt 
      ctcgatgaa gtccggagt gtctttagg cggattcag 
      cacttatcc atacatatc agaacgttc cttttgaag 
      gaataggac tgagtctga attttacat ccaccacta 
      actcaattg tcccaatta actatgtgt cttttctct 
      ctctatctc ttgatgttt gcgtgatgt ctagctgta 
      agtaccacc gttttattc agtttaaaa ctcttggtt 
      gaacgcaca ctaacacaa atatctcag tttttgatt 
      gggacgaaa atggcctac aaacatatc aaggtggag 
      tagatctat catatcaaa ggtgccgca ttaattgta 
      taaagaaac ttatttttt aacgtgttt tcatgtacg 
      ctgtagcat tttgtgaga caggggaac ctcgtgcga 
      ggttttttc aaaatgtga tcgtcatga aagaaactt 
      ttataagat aagtgggag ccaaaattg catgatagt 
      tcgtaatta taagagata ttttaatct aaaacttgt 
      gagcttgat tatccatat cattattgt tagaaagta 
      aagtaagag aatatatta gtgtatagc tagactagt 
      tgaacattt gtcaatgca ctcatcagt gtgttcagc 
      aaaaaaagg gagaaattt cggaaccgc gatcaaaat 
      ctctgctat tacgtacct actagcaac ttagaacca 
      cacctccac cccgaataa ggaaagttt tggaacata 
      tcatagtac tgtctgcga tattttgta aaatttgta 
      gcagtcgta acaaatcgc gatcgtgca gttgataaa 
      tggcaaata tttttgtgg caatgtttt gtgagcgct 
      gctgaggcg tttccagac catttcgag acatatccg 
      gtcgaggtg ttgcaaaac ttcgcgttc ctatgtaag 
      ctcaaaaca aacatgcaa tatttttct aaaaaaatt 
      tctagtgtc aggtcgtta tcgcataga aacgcgaag 
      agcagaccc ggccccacc attttttac aattttacc 
      gtattcatg tgtaatatc gtattaagc actgtaatg 
      gagtacttg tctattcat cattgaaca tggctgttg 
      tgttttgtg tcataatat attcaaatg attatatta 
      aatgtttcc aaaatgtaa tattctatg tatatgtga 
      aagtcaata aacaagttt atgctaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY