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Gene information
General information
TC012257 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4478379..4523835
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012257
    iBeetle screen iB_05202

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    elytra wing veins decreased - Number of animals showing the phenotype: 6

    Comment: wing veins are less prominent, fewer sensillae and their pattern is disturbed

    prosternal process slightly size decreased - Number of animals showing the phenotype: 5


    prosternal process slightly shape thinner - Number of animals showing the phenotype: 5

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012257 RA
      MEVAKTVFL LLVGFQITL TDEIRDTRE GQDVTLECR 
      FSPHNSRET LTYYWAKNN KQTHDNVAI GNVPLDNNY 
      KLNFHPEKG IYDLLISNA SYDRDNGKF ECKVKAGGS 
      GANLHVQRY TLTVLTAPQ EPAIAPGSH VTVTEGKRQ 
      ELTCSSVGG SPDPQVRWY REGSTYPLE AALKNGGSR 
      DQPTTATLS IVPSKEDDE AVFRCEVWN RALPEKRKL 
      VSTVSLSVN YFPRVEVGP ENPLRVERD SQATLQCSV 
      DAKPKVSNI RWTRNGRFI SSNNNHVIH RVSIQDAGK 
      YSCSAENGL GKVGEKEIV LDVLYAPIV ILEAKTKEA 
      EEGESVYIK CNVTANPTP SVIEWVKDG KPDFRQSGD 
      TLRLSHVTA ENSGTYICR AVNMISPSS PPVRRSEKI 
      GNASIALLI RHRPGKARI MPDRPVATE GSPVTLTCT 
      ATPPGWPAP QYRWFRIGS DGQPTILAT GTKYTIPSA 
      NLGTEGVYN CQATNELGP GEMASVNLE VHQPPSFKY 
      QLKPLETKR VGDPNFSMT CSAKGKPKP MVKWLKDND 
      ELTADVNMY EVKTEYSEG HNGAVNVQS VLRFNGKAR 
      PNGNELLPN DRGIYTCSF ENEVKKSES SMHLKIEHE 
      PIVLHQYNK VAYDISETA EVVCKVLAY PKPEFKWFY 
      GSHPTPLQS SSEGHYVIH TSADEDTFT SVLRISDIK 
      KSDYGDYNC QIVNNLGKI DTKIRLQPK GPPEKPTKL 
      VALYTGPNF VTLGWEPGF NGGIANTKY FVSYKKVPG 
      DEDVSIEGC GTVSKSSDW SEVDCQQNV PCNVSHLDQ 
      HQSYLFKVK ALNTKGNSE HSHEVKLTT RVDRIPLPQ 
      RVAYDPASH TVSINVPMT CLPLVAVIE TISTDNHPM 
      PIWQVVETL PLQVSGVSP TYKEANLDQ MGRGYKGVG 
      RSLVDEPIG VNDNYTPRV RVKLCLRSR QEHCGDFVE 
      AELGSSYIR EASALATPT LIAIVVSCI VFVLFVGLL 
      LIFCRCKRN QSKKSQAKD YEMDSVRPT IIPQQNQAP 
      PPYYPSTGM ENKALEHSL DLALAMEDQ KNVYATQNG 
      YGYHVANPD IQGRQNMTN NDWANMGYL ENSYSNSNN 
      GGSVNSQDS LWQMKMAAA STNSTNQLQ PHHINVDRQ 
      NSYGYDPIT HGGYGAVDD YAPYPHITT QSNHGDDYM 
      RNSNNPSRQ DYCSDPYAA VHKPKKRID QHIDSPYHD 
      VSGLPDPYL EQMEDEKPP QHMSLSYEE SLESGYSTP 
      NSRTRRVIR EIIV
    CDS sequence
    • >TC012257 RA
      atggaagtt gcaaagacg gttttcctg ctcctcgta 
      ggcttccaa ataaccttg acggacgaa attagggac 
      accagggaa ggccaagac gtcacgctg gaatgccga 
      ttttcaccc cacaattcc agggagacc ctgacgtat 
      tattgggcg aaaaacaac aagcagacg cacgataat 
      gtggccata ggaaacgtt cctctggat aacaactac 
      aaattgaat tttcatcca gagaagggc atctacgat 
      ctcctaatt tccaacgcc agttacgac agggacaac 
      ggtaaattc gaatgcaaa gttaaagcc ggcggttcc 
      ggggcgaat ttgcatgta cagagatac acccttact 
      gtgttaacg gcccctcaa gaaccagcc attgccccc 
      ggatcccac gttaccgtc actgaagga aagcgccag 
      gagctgacc tgcagcagt gtggggggc tcgcccgac 
      ccccaagtc agatggtac agggaaggc tccacgtac 
      ccgctggaa gccgccctc aagaacggg gggtcgcgc 
      gaccagccc acgaccgcc accctctcc atcgtccct 
      tccaaggag gacgacgag gccgtgttc cggtgcgag 
      gtctggaac agggccttg ccggagaag aggaaactg 
      gtctcgacc gtgtctctc agcgtcaac tatttccct 
      cgcgtagaa gtcggtcca gagaacccc ctgagagtg 
      gagcgcgac tctcaagcc accttacaa tgctcagtt 
      gacgccaag ccgaaagtt tccaacatc cgctggacg 
      cgcaacggc cgcttcatc agcagcaac aacaaccac 
      gtgatccac cgagtgagc atccaggac gctggcaag 
      tactcctgc agcgccgag aacggcctc ggcaaggtg 
      ggcgagaag gagatcgtc ctggacgtg ctctacgcc 
      ccgatcgtc atcctcgag gccaagacg aaggaggcc 
      gaggagggc gagtccgtc tacatcaaa tgcaacgtc 
      accgccaac cccacgccc agcgtcatc gagtgggtc 
      aaagacggc aagccggac ttccggcaa tccggcgac 
      acgctccgg ctgagccac gtgacggcg gaaaactcc 
      gggacgtac atctgccgt gccgtcaac atgatcagc 
      cccagctcg ccgcccgtc cgccggagc gagaagatc 
      ggcaacgcg tccatcgcg ctgctgatc cggcaccgg 
      cccggaaag gcccggatc atgccggac cggcccgtg 
      gccaccgag ggctcgccg gtgacgctg acctgcacg 
      gcgacgccc cccggctgg cccgcgccc cagtaccgc 
      tggttccgc atcggcagc gacgggcag cccaccatc 
      ctcgccacc ggcacgaag tacaccatc cccagcgcc 
      aatctgggc acggagggg gtgtataat tgccaggct 
      accaacgaa ttaggcccc ggcgagatg gcgtcagtc 
      aatttggaa gtccatcag ccccccagc ttcaagtat 
      caattaaaa cctttggag actaaacgt gtcggtgat 
      cccaatttc tccatgacc tgcagcgcg aaaggtaaa 
      ccgaagccg atggttaag tggctgaaa gacaacgac 
      gaactgact gccgacgtg aatatgtat gaagtcaag 
      acggagtat tccgagggc cacaacggc gccgtcaat 
      gtacagagc gtcttgaga ttcaacgga aaggccagg 
      ccgaacggg aacgaattg ttgccgaat gatcgaggg 
      atttacact tgctccttc gagaatgag gtcaaaaag 
      tccgagtcc agcatgcat ctcaagatt gaacatgaa 
      ccgatcgtc ttacatcag tacaacaaa gtcgcctat 
      gacatttct gaaactgcc gaagttgtg tgcaaagtc 
      ctcgcgtat ccgaagccg gaattcaaa tggttttat 
      ggttcacat cctacacct cttcaatca tcttcggaa 
      ggccattac gtgatccac accagcgcc gacgaggac 
      acttttaca agcgttctg cgtatctca gacatcaaa 
      aaatccgat tacggcgat tacaattgc caaatcgtg 
      aacaattta gggaaaatc gatacgaaa attcggctc 
      caacccaaa ggcccgccg gaaaagccc accaaatta 
      gtcgctttg tacaccggc cccaacttc gtgacgctc 
      ggctgggag cccggcttc aacggcggc atcgccaat 
      accaaatat ttcgtctcc tacaaaaag gttcccggc 
      gacgaggac gtgtcgatc gagggctgc ggcaccgtc 
      agcaaatcc agcgattgg tcggaggtc gactgccag 
      cagaacgtc ccctgcaac gtctcgcat ttggaccag 
      caccagagc tatctgttc aaggtcaag gcgctgaac 
      accaaaggc aacagcgag cactcgcac gaggttaag 
      ctaacgacg agagtcgac cggatcccg ctgccccag 
      agggtggcc tacgacccg gcctcgcac accgtctcc 
      atcaacgtc cccatgacc tgcctgccg ctggtggcc 
      gtcatcgag accatcagc accgacaac caccccatg 
      cccatctgg caggtggtg gagacgctc cccttgcag 
      gtctccggg gtctccccc acgtacaag gaggccaac 
      ttggaccag atgggccgg gggtacaag ggggtggga 
      cgctcgctt gtcgacgag ccgatcggc gttaatgac 
      aactacaca ccccgagtg agggttaaa ttgtgtcta 
      aggagccgg caggagcac tgcggggac ttcgtcgag 
      gccgaactc ggatcgtcg tacatccgc gaagcgtct 
      gctctcgcg accccgacc ctaatcgcc atcgtcgtc 
      tcctgcatc gtgttcgtg ctgttcgtc ggcctcctc 
      ctgatcttc tgccgctgc aagcgcaac cagtcgaag 
      aaatcgcaa gccaaggac tacgagatg gactcggtg 
      cggcccacg atcatcccg cagcagaac caagcgccc 
      ccgccttac tacccctcg accgggatg gagaacaag 
      gcgcttgag cactccctg gacctggct ttggcgatg 
      gaggaccag aagaacgtc tacgccacg cagaacggc 
      tacggctac cacgtggcc aaccccgac atccagggg 
      cggcagaac atgaccaac aacgactgg gccaatatg 
      ggctatttg gaaaacagt tattcaaat tcgaataac 
      ggcggcagc gttaattcc caggactcg ttgtggcag 
      atgaaaatg gccgcagct tcgaccaac tcgaccaac 
      cagctccag cctcatcat atcaatgtt gataggcag 
      aattcctac ggatatgat cccataacc catggaggt 
      tacggggca gttgatgac tatgctccc tatccgcac 
      ataacgacg caaagtaat cacggggat gactatatg 
      agaaatagc aacaatccg tccaggcaa gactattgc 
      agtgatccc tatgccgcc gtgcacaag cccaagaag 
      aggatcgac caacatatt gactcccca tatcatgat 
      gtcagcggc cttccagat ccatacttg gagcagatg 
      gaggacgag aagccgccc caacacatg tcactaagt 
      tatgaggaa agtttagaa agtggatat tccactccg 
      aattcgcga acaagaaga gtaatccga gagataatt 
      gtctaa
    mRNA sequence
    • >TC012257 RA
      ccgtttgga agcgagcgc acgcagcgc tgccaacgg 
      tcgcaactt caaatccgt tggcgcgct cattcctcc 
      gcacaacct cgcgcaaca aggatatag tgttgccgg 
      ccagcgagc gcatttttt cgaacgctg taaagttgc 
      gagatctgt gtttgtgtc gttggtgct tttgttctt 
      tctgatgtt cgattatcg gaaaaaggt gccaggatg 
      gattaagtg gtggcgggt tttgtctcg ttgtgactg 
      cgatcagcc ctccggatg agggtttaa agtgcgcgt 
      ggtgtgtat tggattttt tctcttatc ttcgctcga 
      caacgaact tttaacgaa tcatggaag ttgcaaaga 
      cggttttcc tgctcctcg taggcttcc aaataacct 
      tgacggacg aaattaggg acaccaggg aaggccaag 
      acgtcacgc tggaatgcc gattttcac cccacaatt 
      ccagggaga ccctgacgt attattggg cgaaaaaca 
      acaagcaga cgcacgata atgtggcca taggaaacg 
      ttcctctgg ataacaact acaaattga attttcatc 
      cagagaagg gcatctacg atctcctaa tttccaacg 
      ccagttacg acagggaca acggtaaat tcgaatgca 
      aagttaaag ccggcggtt ccggggcga atttgcatg 
      tacagagat acaccctta ctgtgttaa cggcccctc 
      aagaaccag ccattgccc ccggatccc acgttaccg 
      tcactgaag gaaagcgcc aggagctga cctgcagca 
      gtgtggggg gctcgcccg acccccaag tcagatggt 
      acagggaag gctccacgt acccgctgg aagccgccc 
      tcaagaacg gggggtcgc gcgaccagc ccacgaccg 
      ccaccctct ccatcgtcc cttccaagg aggacgacg 
      aggccgtgt tccggtgcg aggtctgga acagggcct 
      tgccggaga agaggaaac tggtctcga ccgtgtctc 
      tcagcgtca actatttcc ctcgcgtag aagtcggtc 
      cagagaacc ccctgagag tggagcgcg actctcaag 
      ccaccttac aatgctcag ttgacgcca agccgaaag 
      tttccaaca tccgctgga cgcgcaacg gccgcttca 
      tcagcagca acaacaacc acgtgatcc accgagtga 
      gcatccagg acgctggca agtactcct gcagcgccg 
      agaacggcc tcggcaagg tgggcgaga aggagatcg 
      tcctggacg tgctctacg ccccgatcg tcatcctcg 
      aggccaaga cgaaggagg ccgaggagg gcgagtccg 
      tctacatca aatgcaacg tcaccgcca accccacgc 
      ccagcgtca tcgagtggg tcaaagacg gcaagccgg 
      acttccggc aatccggcg acacgctcc ggctgagcc 
      acgtgacgg cggaaaact ccgggacgt acatctgcc 
      gtgccgtca acatgatca gccccagct cgccgcccg 
      tccgccgga gcgagaaga tcggcaacg cgtccatcg 
      cgctgctga tccggcacc ggcccggaa aggcccgga 
      tcatgccgg accggcccg tggccaccg agggctcgc 
      cggtgacgc tgacctgca cggcgacgc cccccggct 
      ggcccgcgc cccagtacc gctggttcc gcatcggca 
      gcgacgggc agcccacca tcctcgcca ccggcacga 
      agtacacca tccccagcg ccaatctgg gcacggagg 
      gggtgtata attgccagg ctaccaacg aattaggcc 
      ccggcgaga tggcgtcag tcaatttgg aagtccatc 
      agcccccca gcttcaagt atcaattaa aacctttgg 
      agactaaac gtgtcggtg atcccaatt tctccatga 
      cctgcagcg cgaaaggta aaccgaagc cgatggtta 
      agtggctga aagacaacg acgaactga ctgccgacg 
      tgaatatgt atgaagtca agacggagt attccgagg 
      gccacaacg gcgccgtca atgtacaga gcgtcttga 
      gattcaacg gaaaggcca ggccgaacg ggaacgaat 
      tgttgccga atgatcgag ggatttaca cttgctcct 
      tcgagaatg aggtcaaaa agtccgagt ccagcatgc 
      atctcaaga ttgaacatg aaccgatcg tcttacatc 
      agtacaaca aagtcgcct atgacattt ctgaaactg 
      ccgaagttg tgtgcaaag tcctcgcgt atccgaagc 
      cggaattca aatggtttt atggttcac atcctacac 
      ctcttcaat catcttcgg aaggccatt acgtgatcc 
      acaccagcg ccgacgagg acactttta caagcgttc 
      tgcgtatct cagacatca aaaaatccg attacggcg 
      attacaatt gccaaatcg tgaacaatt tagggaaaa 
      tcgatacga aaattcggc tccaaccca aaggcccgc 
      cggaaaagc ccaccaaat tagtcgctt tgtacaccg 
      gccccaact tcgtgacgc tcggctggg agcccggct 
      tcaacggcg gcatcgcca ataccaaat atttcgtct 
      cctacaaaa aggttcccg gcgacgagg acgtgtcga 
      tcgagggct gcggcaccg tcagcaaat ccagcgatt 
      ggtcggagg tcgactgcc agcagaacg tcccctgca 
      acgtctcgc atttggacc agcaccaga gctatctgt 
      tcaaggtca aggcgctga acaccaaag gcaacagcg 
      agcactcgc acgaggtta agctaacga cgagagtcg 
      accggatcc cgctgcccc agagggtgg cctacgacc 
      cggcctcgc acaccgtct ccatcaacg tccccatga 
      cctgcctgc cgctggtgg ccgtcatcg agaccatca 
      gcaccgaca accacccca tgcccatct ggcaggtgg 
      tggagacgc tccccttgc aggtctccg gggtctccc 
      ccacgtaca aggaggcca acttggacc agatgggcc 
      gggggtaca agggggtgg gacgctcgc ttgtcgacg 
      agccgatcg gcgttaatg acaactaca caccccgag 
      tgagggtta aattgtgtc taaggagcc ggcaggagc 
      actgcgggg acttcgtcg aggccgaac tcggatcgt 
      cgtacatcc gcgaagcgt ctgctctcg cgaccccga 
      ccctaatcg ccatcgtcg tctcctgca tcgtgttcg 
      tgctgttcg tcggcctcc tcctgatct tctgccgct 
      gcaagcgca accagtcga agaaatcgc aagccaagg 
      actacgaga tggactcgg tgcggccca cgatcatcc 
      cgcagcaga accaagcgc ccccgcctt actacccct 
      cgaccggga tggagaaca aggcgcttg agcactccc 
      tggacctgg ctttggcga tggaggacc agaagaacg 
      tctacgcca cgcagaacg gctacggct accacgtgg 
      ccaaccccg acatccagg ggcggcaga acatgacca 
      acaacgact gggccaata tgggctatt tggaaaaca 
      gttattcaa attcgaata acggcggca gcgttaatt 
      cccaggact cgttgtggc agatgaaaa tggccgcag 
      cttcgacca actcgacca accagctcc agcctcatc 
      atatcaatg ttgataggc agaattcct acggatatg 
      atcccataa cccatggag gttacgggg cagttgatg 
      actatgctc cctatccgc acataacga cgcaaagta 
      atcacgggg atgactata tgagaaata gcaacaatc 
      cgtccaggc aagactatt gcagtgatc cctatgccg 
      ccgtgcaca agcccaaga agaggatcg accaacata 
      ttgactccc catatcatg atgtcagcg gccttccag 
      atccatact tggagcaga tggaggacg agaagccgc 
      cccaacaca tgtcactaa gttatgagg aaagtttag 
      aaagtggat attccactc cgaattcgc gaacaagaa 
      gagtaatcc gagagataa ttgtctaag tgttatttt 
      tgatcgatt tttgaaaag gacttagtt tcccgtgat 
      cgtgcaaat tgttttctt aattttcga aattatctt 
      cttgagtac ctgaagcca cgttattat ttttcactt 
      tagtgtttt aatgtttgt gtgtgtatg tggactgtt 
      ttaactgcc attttgtgt cacaaattt tgccattcg 
      aaagccaaa tgtggacta acaaacttt ttaaaagac 
      gaatttttc aaagaatgt gattatgat tgcaataag 
      tatttgttg tgtaatgtt attgtattg tttttgata 
      ttgtgagta ttacacatt ttattgctt ttcgcatac 
      tgtgcctta aagactggt aaataagcg catttgcca 
      ctttttaaa gtgcgtgat attttttta tcgctgagc 
      tttattttg tagaaggaa atgtgccaa aggaaagtg 
      atacaatgt gtaatatgg ttttatttt tgtgttgtc 
      gtgttgcaa ttttactta tgtaggaac tcctatttt 
      caaaattct caagctcaa atatttttt attttattt 
      ttcacaagt gatctcaca agatttata ttgaaatgc 
      cgatttatg aaaaaatat attgactcg atttttgtt 
      gtatattat tttgtacat atattaaga aaagactat 
      tattttgta aatgtaaga ctagacgct aagattatt 
      attattata ctcgaattt taatgttgt aaataatac 
      cccataata agtttttta tttgtatca ttcattttt 
      gtaaataaa tgtgtatta aaaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY