| General information | |||
| TC012257 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 4478379..4523835 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012257 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05202 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)
| Comment: wing veins are less prominent, fewer sensillae and their pattern is disturbed |
prosternal process slightly size decreased - Number of animals showing the phenotype: 5
prosternal process slightly shape thinner - Number of animals showing the phenotype: 5
- >TC012257 RA
MEVAKTVFL LLVGFQITL TDEIRDTRE GQDVTLECR FSPHNSRET LTYYWAKNN KQTHDNVAI GNVPLDNNY KLNFHPEKG IYDLLISNA SYDRDNGKF ECKVKAGGS GANLHVQRY TLTVLTAPQ EPAIAPGSH VTVTEGKRQ ELTCSSVGG SPDPQVRWY REGSTYPLE AALKNGGSR DQPTTATLS IVPSKEDDE AVFRCEVWN RALPEKRKL VSTVSLSVN YFPRVEVGP ENPLRVERD SQATLQCSV DAKPKVSNI RWTRNGRFI SSNNNHVIH RVSIQDAGK YSCSAENGL GKVGEKEIV LDVLYAPIV ILEAKTKEA EEGESVYIK CNVTANPTP SVIEWVKDG KPDFRQSGD TLRLSHVTA ENSGTYICR AVNMISPSS PPVRRSEKI GNASIALLI RHRPGKARI MPDRPVATE GSPVTLTCT ATPPGWPAP QYRWFRIGS DGQPTILAT GTKYTIPSA NLGTEGVYN CQATNELGP GEMASVNLE VHQPPSFKY QLKPLETKR VGDPNFSMT CSAKGKPKP MVKWLKDND ELTADVNMY EVKTEYSEG HNGAVNVQS VLRFNGKAR PNGNELLPN DRGIYTCSF ENEVKKSES SMHLKIEHE PIVLHQYNK VAYDISETA EVVCKVLAY PKPEFKWFY GSHPTPLQS SSEGHYVIH TSADEDTFT SVLRISDIK KSDYGDYNC QIVNNLGKI DTKIRLQPK GPPEKPTKL VALYTGPNF VTLGWEPGF NGGIANTKY FVSYKKVPG DEDVSIEGC GTVSKSSDW SEVDCQQNV PCNVSHLDQ HQSYLFKVK ALNTKGNSE HSHEVKLTT RVDRIPLPQ RVAYDPASH TVSINVPMT CLPLVAVIE TISTDNHPM PIWQVVETL PLQVSGVSP TYKEANLDQ MGRGYKGVG RSLVDEPIG VNDNYTPRV RVKLCLRSR QEHCGDFVE AELGSSYIR EASALATPT LIAIVVSCI VFVLFVGLL LIFCRCKRN QSKKSQAKD YEMDSVRPT IIPQQNQAP PPYYPSTGM ENKALEHSL DLALAMEDQ KNVYATQNG YGYHVANPD IQGRQNMTN NDWANMGYL ENSYSNSNN GGSVNSQDS LWQMKMAAA STNSTNQLQ PHHINVDRQ NSYGYDPIT HGGYGAVDD YAPYPHITT QSNHGDDYM RNSNNPSRQ DYCSDPYAA VHKPKKRID QHIDSPYHD VSGLPDPYL EQMEDEKPP QHMSLSYEE SLESGYSTP NSRTRRVIR EIIV
- >TC012257 RA
atggaagtt gcaaagacg gttttcctg ctcctcgta ggcttccaa ataaccttg acggacgaa attagggac accagggaa ggccaagac gtcacgctg gaatgccga ttttcaccc cacaattcc agggagacc ctgacgtat tattgggcg aaaaacaac aagcagacg cacgataat gtggccata ggaaacgtt cctctggat aacaactac aaattgaat tttcatcca gagaagggc atctacgat ctcctaatt tccaacgcc agttacgac agggacaac ggtaaattc gaatgcaaa gttaaagcc ggcggttcc ggggcgaat ttgcatgta cagagatac acccttact gtgttaacg gcccctcaa gaaccagcc attgccccc ggatcccac gttaccgtc actgaagga aagcgccag gagctgacc tgcagcagt gtggggggc tcgcccgac ccccaagtc agatggtac agggaaggc tccacgtac ccgctggaa gccgccctc aagaacggg gggtcgcgc gaccagccc acgaccgcc accctctcc atcgtccct tccaaggag gacgacgag gccgtgttc cggtgcgag gtctggaac agggccttg ccggagaag aggaaactg gtctcgacc gtgtctctc agcgtcaac tatttccct cgcgtagaa gtcggtcca gagaacccc ctgagagtg gagcgcgac tctcaagcc accttacaa tgctcagtt gacgccaag ccgaaagtt tccaacatc cgctggacg cgcaacggc cgcttcatc agcagcaac aacaaccac gtgatccac cgagtgagc atccaggac gctggcaag tactcctgc agcgccgag aacggcctc ggcaaggtg ggcgagaag gagatcgtc ctggacgtg ctctacgcc ccgatcgtc atcctcgag gccaagacg aaggaggcc gaggagggc gagtccgtc tacatcaaa tgcaacgtc accgccaac cccacgccc agcgtcatc gagtgggtc aaagacggc aagccggac ttccggcaa tccggcgac acgctccgg ctgagccac gtgacggcg gaaaactcc gggacgtac atctgccgt gccgtcaac atgatcagc cccagctcg ccgcccgtc cgccggagc gagaagatc ggcaacgcg tccatcgcg ctgctgatc cggcaccgg cccggaaag gcccggatc atgccggac cggcccgtg gccaccgag ggctcgccg gtgacgctg acctgcacg gcgacgccc cccggctgg cccgcgccc cagtaccgc tggttccgc atcggcagc gacgggcag cccaccatc ctcgccacc ggcacgaag tacaccatc cccagcgcc aatctgggc acggagggg gtgtataat tgccaggct accaacgaa ttaggcccc ggcgagatg gcgtcagtc aatttggaa gtccatcag ccccccagc ttcaagtat caattaaaa cctttggag actaaacgt gtcggtgat cccaatttc tccatgacc tgcagcgcg aaaggtaaa ccgaagccg atggttaag tggctgaaa gacaacgac gaactgact gccgacgtg aatatgtat gaagtcaag acggagtat tccgagggc cacaacggc gccgtcaat gtacagagc gtcttgaga ttcaacgga aaggccagg ccgaacggg aacgaattg ttgccgaat gatcgaggg atttacact tgctccttc gagaatgag gtcaaaaag tccgagtcc agcatgcat ctcaagatt gaacatgaa ccgatcgtc ttacatcag tacaacaaa gtcgcctat gacatttct gaaactgcc gaagttgtg tgcaaagtc ctcgcgtat ccgaagccg gaattcaaa tggttttat ggttcacat cctacacct cttcaatca tcttcggaa ggccattac gtgatccac accagcgcc gacgaggac acttttaca agcgttctg cgtatctca gacatcaaa aaatccgat tacggcgat tacaattgc caaatcgtg aacaattta gggaaaatc gatacgaaa attcggctc caacccaaa ggcccgccg gaaaagccc accaaatta gtcgctttg tacaccggc cccaacttc gtgacgctc ggctgggag cccggcttc aacggcggc atcgccaat accaaatat ttcgtctcc tacaaaaag gttcccggc gacgaggac gtgtcgatc gagggctgc ggcaccgtc agcaaatcc agcgattgg tcggaggtc gactgccag cagaacgtc ccctgcaac gtctcgcat ttggaccag caccagagc tatctgttc aaggtcaag gcgctgaac accaaaggc aacagcgag cactcgcac gaggttaag ctaacgacg agagtcgac cggatcccg ctgccccag agggtggcc tacgacccg gcctcgcac accgtctcc atcaacgtc cccatgacc tgcctgccg ctggtggcc gtcatcgag accatcagc accgacaac caccccatg cccatctgg caggtggtg gagacgctc cccttgcag gtctccggg gtctccccc acgtacaag gaggccaac ttggaccag atgggccgg gggtacaag ggggtggga cgctcgctt gtcgacgag ccgatcggc gttaatgac aactacaca ccccgagtg agggttaaa ttgtgtcta aggagccgg caggagcac tgcggggac ttcgtcgag gccgaactc ggatcgtcg tacatccgc gaagcgtct gctctcgcg accccgacc ctaatcgcc atcgtcgtc tcctgcatc gtgttcgtg ctgttcgtc ggcctcctc ctgatcttc tgccgctgc aagcgcaac cagtcgaag aaatcgcaa gccaaggac tacgagatg gactcggtg cggcccacg atcatcccg cagcagaac caagcgccc ccgccttac tacccctcg accgggatg gagaacaag gcgcttgag cactccctg gacctggct ttggcgatg gaggaccag aagaacgtc tacgccacg cagaacggc tacggctac cacgtggcc aaccccgac atccagggg cggcagaac atgaccaac aacgactgg gccaatatg ggctatttg gaaaacagt tattcaaat tcgaataac ggcggcagc gttaattcc caggactcg ttgtggcag atgaaaatg gccgcagct tcgaccaac tcgaccaac cagctccag cctcatcat atcaatgtt gataggcag aattcctac ggatatgat cccataacc catggaggt tacggggca gttgatgac tatgctccc tatccgcac ataacgacg caaagtaat cacggggat gactatatg agaaatagc aacaatccg tccaggcaa gactattgc agtgatccc tatgccgcc gtgcacaag cccaagaag aggatcgac caacatatt gactcccca tatcatgat gtcagcggc cttccagat ccatacttg gagcagatg gaggacgag aagccgccc caacacatg tcactaagt tatgaggaa agtttagaa agtggatat tccactccg aattcgcga acaagaaga gtaatccga gagataatt gtctaa
- >TC012257 RA
ccgtttgga agcgagcgc acgcagcgc tgccaacgg tcgcaactt caaatccgt tggcgcgct cattcctcc gcacaacct cgcgcaaca aggatatag tgttgccgg ccagcgagc gcatttttt cgaacgctg taaagttgc gagatctgt gtttgtgtc gttggtgct tttgttctt tctgatgtt cgattatcg gaaaaaggt gccaggatg gattaagtg gtggcgggt tttgtctcg ttgtgactg cgatcagcc ctccggatg agggtttaa agtgcgcgt ggtgtgtat tggattttt tctcttatc ttcgctcga caacgaact tttaacgaa tcatggaag ttgcaaaga cggttttcc tgctcctcg taggcttcc aaataacct tgacggacg aaattaggg acaccaggg aaggccaag acgtcacgc tggaatgcc gattttcac cccacaatt ccagggaga ccctgacgt attattggg cgaaaaaca acaagcaga cgcacgata atgtggcca taggaaacg ttcctctgg ataacaact acaaattga attttcatc cagagaagg gcatctacg atctcctaa tttccaacg ccagttacg acagggaca acggtaaat tcgaatgca aagttaaag ccggcggtt ccggggcga atttgcatg tacagagat acaccctta ctgtgttaa cggcccctc aagaaccag ccattgccc ccggatccc acgttaccg tcactgaag gaaagcgcc aggagctga cctgcagca gtgtggggg gctcgcccg acccccaag tcagatggt acagggaag gctccacgt acccgctgg aagccgccc tcaagaacg gggggtcgc gcgaccagc ccacgaccg ccaccctct ccatcgtcc cttccaagg aggacgacg aggccgtgt tccggtgcg aggtctgga acagggcct tgccggaga agaggaaac tggtctcga ccgtgtctc tcagcgtca actatttcc ctcgcgtag aagtcggtc cagagaacc ccctgagag tggagcgcg actctcaag ccaccttac aatgctcag ttgacgcca agccgaaag tttccaaca tccgctgga cgcgcaacg gccgcttca tcagcagca acaacaacc acgtgatcc accgagtga gcatccagg acgctggca agtactcct gcagcgccg agaacggcc tcggcaagg tgggcgaga aggagatcg tcctggacg tgctctacg ccccgatcg tcatcctcg aggccaaga cgaaggagg ccgaggagg gcgagtccg tctacatca aatgcaacg tcaccgcca accccacgc ccagcgtca tcgagtggg tcaaagacg gcaagccgg acttccggc aatccggcg acacgctcc ggctgagcc acgtgacgg cggaaaact ccgggacgt acatctgcc gtgccgtca acatgatca gccccagct cgccgcccg tccgccgga gcgagaaga tcggcaacg cgtccatcg cgctgctga tccggcacc ggcccggaa aggcccgga tcatgccgg accggcccg tggccaccg agggctcgc cggtgacgc tgacctgca cggcgacgc cccccggct ggcccgcgc cccagtacc gctggttcc gcatcggca gcgacgggc agcccacca tcctcgcca ccggcacga agtacacca tccccagcg ccaatctgg gcacggagg gggtgtata attgccagg ctaccaacg aattaggcc ccggcgaga tggcgtcag tcaatttgg aagtccatc agcccccca gcttcaagt atcaattaa aacctttgg agactaaac gtgtcggtg atcccaatt tctccatga cctgcagcg cgaaaggta aaccgaagc cgatggtta agtggctga aagacaacg acgaactga ctgccgacg tgaatatgt atgaagtca agacggagt attccgagg gccacaacg gcgccgtca atgtacaga gcgtcttga gattcaacg gaaaggcca ggccgaacg ggaacgaat tgttgccga atgatcgag ggatttaca cttgctcct tcgagaatg aggtcaaaa agtccgagt ccagcatgc atctcaaga ttgaacatg aaccgatcg tcttacatc agtacaaca aagtcgcct atgacattt ctgaaactg ccgaagttg tgtgcaaag tcctcgcgt atccgaagc cggaattca aatggtttt atggttcac atcctacac ctcttcaat catcttcgg aaggccatt acgtgatcc acaccagcg ccgacgagg acactttta caagcgttc tgcgtatct cagacatca aaaaatccg attacggcg attacaatt gccaaatcg tgaacaatt tagggaaaa tcgatacga aaattcggc tccaaccca aaggcccgc cggaaaagc ccaccaaat tagtcgctt tgtacaccg gccccaact tcgtgacgc tcggctggg agcccggct tcaacggcg gcatcgcca ataccaaat atttcgtct cctacaaaa aggttcccg gcgacgagg acgtgtcga tcgagggct gcggcaccg tcagcaaat ccagcgatt ggtcggagg tcgactgcc agcagaacg tcccctgca acgtctcgc atttggacc agcaccaga gctatctgt tcaaggtca aggcgctga acaccaaag gcaacagcg agcactcgc acgaggtta agctaacga cgagagtcg accggatcc cgctgcccc agagggtgg cctacgacc cggcctcgc acaccgtct ccatcaacg tccccatga cctgcctgc cgctggtgg ccgtcatcg agaccatca gcaccgaca accacccca tgcccatct ggcaggtgg tggagacgc tccccttgc aggtctccg gggtctccc ccacgtaca aggaggcca acttggacc agatgggcc gggggtaca agggggtgg gacgctcgc ttgtcgacg agccgatcg gcgttaatg acaactaca caccccgag tgagggtta aattgtgtc taaggagcc ggcaggagc actgcgggg acttcgtcg aggccgaac tcggatcgt cgtacatcc gcgaagcgt ctgctctcg cgaccccga ccctaatcg ccatcgtcg tctcctgca tcgtgttcg tgctgttcg tcggcctcc tcctgatct tctgccgct gcaagcgca accagtcga agaaatcgc aagccaagg actacgaga tggactcgg tgcggccca cgatcatcc cgcagcaga accaagcgc ccccgcctt actacccct cgaccggga tggagaaca aggcgcttg agcactccc tggacctgg ctttggcga tggaggacc agaagaacg tctacgcca cgcagaacg gctacggct accacgtgg ccaaccccg acatccagg ggcggcaga acatgacca acaacgact gggccaata tgggctatt tggaaaaca gttattcaa attcgaata acggcggca gcgttaatt cccaggact cgttgtggc agatgaaaa tggccgcag cttcgacca actcgacca accagctcc agcctcatc atatcaatg ttgataggc agaattcct acggatatg atcccataa cccatggag gttacgggg cagttgatg actatgctc cctatccgc acataacga cgcaaagta atcacgggg atgactata tgagaaata gcaacaatc cgtccaggc aagactatt gcagtgatc cctatgccg ccgtgcaca agcccaaga agaggatcg accaacata ttgactccc catatcatg atgtcagcg gccttccag atccatact tggagcaga tggaggacg agaagccgc cccaacaca tgtcactaa gttatgagg aaagtttag aaagtggat attccactc cgaattcgc gaacaagaa gagtaatcc gagagataa ttgtctaag tgttatttt tgatcgatt tttgaaaag gacttagtt tcccgtgat cgtgcaaat tgttttctt aattttcga aattatctt cttgagtac ctgaagcca cgttattat ttttcactt tagtgtttt aatgtttgt gtgtgtatg tggactgtt ttaactgcc attttgtgt cacaaattt tgccattcg aaagccaaa tgtggacta acaaacttt ttaaaagac gaatttttc aaagaatgt gattatgat tgcaataag tatttgttg tgtaatgtt attgtattg tttttgata ttgtgagta ttacacatt ttattgctt ttcgcatac tgtgcctta aagactggt aaataagcg catttgcca ctttttaaa gtgcgtgat attttttta tcgctgagc tttattttg tagaaggaa atgtgccaa aggaaagtg atacaatgt gtaatatgg ttttatttt tgtgttgtc gtgttgcaa ttttactta tgtaggaac tcctatttt caaaattct caagctcaa atatttttt attttattt ttcacaagt gatctcaca agatttata ttgaaatgc cgatttatg aaaaaatat attgactcg atttttgtt gtatattat tttgtacat atattaaga aaagactat tattttgta aatgtaaga ctagacgct aagattatt attattata ctcgaattt taatgttgt aaataatac cccataata agtttttta tttgtatca ttcattttt gtaaataaa tgtgtatta aaaac
