Status
Please wait ...
Gene information
General information
TC012250 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4648049..4652757
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012250
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05199

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012250 RA
      MSSKLEIQW TPITNKFIT WGSEICLYE VHQDRDNVL 
      QNIKLSSTT SANLLATNT NHHYVKCLD VYPKSDADL 
      LLAVGLSNG RISLSTFGP TEYDALGLT GKELVPRHA 
      RQCNAVCWN TIETSWVAA GLDKYRADN SVLIWDIMK 
      SSQNNESNS GRMAVNTMA PGVELAKPI AEFGVSETA 
      HSMAWFSSN SKVIAVGMN LKNIKFVDI RDPNKITNS 
      TLTKAVFGV CMDPNDDRH LASFFENNI YVWDTRNFE 
      KPILTLHQN KPVIKIAWC PTKYNLLGS LQKESSVIN 
      LYDIQHTVI GNEEVEPSV LERDVVPGS PHNIMSFSW 
      HNSDENRFL TIALSGTIT DYTVFDRIT LNWAPTSNV 
      VWTYGRKTM KYINDCPLQ DISTKIRQR ALSGYGLKA 
      EIYKNGEVV DNEMLANVW NWLFLSGKL VEDGVLTGT 
      QCKHPGVQA VLKLDPSNN KSDHYSLLW SDLGNKNCQ 
      GVVRIFKHE DRDKALHLC SWHFERDSN QLTAFLEQL 
      EKEGAHTRA AAIAVFSLK IRLAIEILS RAPETLAFG 
      LGLNVVAMA LAGFTDDKN SVWKQFCST AKQKLSDPY 
      LKVIFSFLT AENYNFESV LNESGVTVD DRVAFACMF 
      LPDNRLHDY LKKLSDKLI EEGNLDGLL LTGTNQDGI 
      KLLQRYLDA TGDIQSTAL IAVRAFPVE QFSEVIKVW 
      ISSYRNLLD TWQLWNERA HFDIMLSTY CPADKPPQQ 
      VYVSCNYCG KTISAYMYG LTRGRGPFT RIGGTANKL 
      KMSSCPGCR KPLPRCAIC LMHMGTTTG NFSNEVDSN 
      KIIEFSNWF TWCQTCRHG GHVSHMTHW FDEHSECPV 
      TACTCKCFS VDSLSNAII K
    CDS sequence
    • >TC012250 RA
      atgtcctcg aaactggaa atccagtgg acccccatc 
      accaacaaa tttatcacg tggggttcc gaaatttgc 
      ttgtacgag gtgcaccaa gaccgcgat aacgttttg 
      caaaatatt aagctatca agcacgact tccgccaat 
      ttactggcg acaaacacc aaccaccac tacgttaaa 
      tgcctggat gtttaccca aaatccgat gccgatttg 
      ttgctagct gtgggttta tcaaatgga cgaatatca 
      ctgtcgaca ttcggcccc acggagtat gacgccttg 
      gggttgact gggaaagaa ctggtgccg agacatgca 
      aggcagtgt aatgccgtg tgctggaac acaattgag 
      actagttgg gtggctgcc gggttagac aagtacaga 
      gcggacaat tccgttctc atttgggac attatgaag 
      tcgtcccaa aacaacgaa agcaattcg ggaaggatg 
      gcggttaac accatggcc ccaggggtg gagctcgca 
      aaacccata gccgaattt ggggtttcc gaaaccgcc 
      cacagcatg gcctggttc agcagcaac agcaaggtg 
      attgctgtc gggatgaac ttgaaaaat atcaagttt 
      gtcgacatc agagatcct aacaaaatc acaaattct 
      acgctaacc aaggccgtt tttggcgtt tgcatggac 
      ccgaacgac gaccggcat ttggcgtca tttttcgag 
      aataatatt tacgtttgg gatacgaga aatttcgaa 
      aaacccatc ctgacgctg caccagaac aagcctgtg 
      atcaaaatt gcctggtgc cccacaaag tacaatctt 
      ttggggtct ttgcaaaaa gagagctcc gtgattaac 
      ttatacgac atacagcac actgtaatc gggaacgag 
      gaagtggaa ccgtccgtt ttggagcgg gacgtggtc 
      ccgggatcc ccccacaac ataatgtca ttttcgtgg 
      cacaactcc gacgagaac cgatttctg acgatagca 
      ttgtcgggg accataaca gactacaca gttttcgac 
      agaataacc ctcaactgg gcccccacg tccaacgtg 
      gtgtggact tacggacgc aaaaccatg aagtacatc 
      aacgactgt cccctgcag gacatctcg accaaaatc 
      cgacaaagg gcgctgtcc ggctacggc ttaaaagca 
      gagatttac aaaaacggc gaagttgtg gacaacgaa 
      atgctggca aacgtgtgg aactggctg tttttgagc 
      gggaagttg gtggaagac ggggttttg accggaaca 
      cagtgcaaa cacccgggg gtgcaagcg gttttaaaa 
      cttgacccc agtaacaac aagtctgat cactattcc 
      ttgctgtgg tccgatctg gggaacaaa aactgccaa 
      ggagttgtc cgaattttc aagcacgag gaccgggac 
      aaggcgttg catttgtgc agttggcat tttgagaga 
      gattcaaac cagctgacc gcctttttg gagcagctt 
      gaaaaagaa ggggcgcac acaagagct gcagcgatc 
      gcggttttt agtttaaaa atccggctc gcgattgaa 
      attttgagt cgggctccg gaaaccctg gccttcggg 
      ttggggcta aatgttgta gcaatggca cttgcgggg 
      ttcaccgac gataagaat agtgtctgg aaacagttc 
      tgttcgacg gcgaagcaa aaactgtcc gatccgtat 
      ttgaaagtg atttttagt ttcttgact gctgagaat 
      tacaatttc gagagcgtt ttgaacgag agtggggta 
      acagtcgac gatcgagtc gcttttgct tgcatgttt 
      ttgcccgac aaccgactg cacgactat ttaaagaaa 
      ttgtcagat aagttgata gaagagggg aatttggac 
      ggtttattg ctcacagga acaaaccag gacgggatt 
      aagttgttg caaagatat ttggacgcc actggtgat 
      atccaatcg actgctctc atagccgtc cgtgctttt 
      cccgtggaa caattctcc gaagttatc aaagtctgg 
      atctcgagt taccgcaac ctgcttgat acttggcag 
      ttgtggaac gaacgagcc cactttgac atcatgttg 
      agtacttac tgccccgcc gacaagccc ccacagcaa 
      gtgtacgtt tcgtgcaat tactgcggc aagaccatc 
      tcggcctac atgtacggg ctcaccagg gggcggggc 
      cccttcact cgcattggc ggcaccgcc aacaaactc 
      aagatgtcg tcatgcccc ggttgtcgg aaaccgctg 
      ccgcggtgt gcgatatgt ctcatgcat atgggcacc 
      accacaggg aatttcagc aacgaggtc gatagtaat 
      aaaattatt gaattttcc aactggttc acgtggtgt 
      cagacgtgc aggcatggc gggcacgtg tctcacatg 
      acccactgg tttgacgag cattcggaa tgtcctgtg 
      accgcctgc acttgcaaa tgcttttct gttgattcg 
      ctcagcaat gcgattatt aagtga
    mRNA sequence
    • >TC012250 RA
      ttctaagta tttaattac cgttttagc ttccataaa 
      atgtcctcg aaactggaa atccagtgg acccccatc 
      accaacaaa tttatcacg tggggttcc gaaatttgc 
      ttgtacgag gtgcaccaa gaccgcgat aacgttttg 
      caaaatatt aagctatca agcacgact tccgccaat 
      ttactggcg acaaacacc aaccaccac tacgttaaa 
      tgcctggat gtttaccca aaatccgat gccgatttg 
      ttgctagct gtgggttta tcaaatgga cgaatatca 
      ctgtcgaca ttcggcccc acggagtat gacgccttg 
      gggttgact gggaaagaa ctggtgccg agacatgca 
      aggcagtgt aatgccgtg tgctggaac acaattgag 
      actagttgg gtggctgcc gggttagac aagtacaga 
      gcggacaat tccgttctc atttgggac attatgaag 
      tcgtcccaa aacaacgaa agcaattcg ggaaggatg 
      gcggttaac accatggcc ccaggggtg gagctcgca 
      aaacccata gccgaattt ggggtttcc gaaaccgcc 
      cacagcatg gcctggttc agcagcaac agcaaggtg 
      attgctgtc gggatgaac ttgaaaaat atcaagttt 
      gtcgacatc agagatcct aacaaaatc acaaattct 
      acgctaacc aaggccgtt tttggcgtt tgcatggac 
      ccgaacgac gaccggcat ttggcgtca tttttcgag 
      aataatatt tacgtttgg gatacgaga aatttcgaa 
      aaacccatc ctgacgctg caccagaac aagcctgtg 
      atcaaaatt gcctggtgc cccacaaag tacaatctt 
      ttggggtct ttgcaaaaa gagagctcc gtgattaac 
      ttatacgac atacagcac actgtaatc gggaacgag 
      gaagtggaa ccgtccgtt ttggagcgg gacgtggtc 
      ccgggatcc ccccacaac ataatgtca ttttcgtgg 
      cacaactcc gacgagaac cgatttctg acgatagca 
      ttgtcgggg accataaca gactacaca gttttcgac 
      agaataacc ctcaactgg gcccccacg tccaacgtg 
      gtgtggact tacggacgc aaaaccatg aagtacatc 
      aacgactgt cccctgcag gacatctcg accaaaatc 
      cgacaaagg gcgctgtcc ggctacggc ttaaaagca 
      gagatttac aaaaacggc gaagttgtg gacaacgaa 
      atgctggca aacgtgtgg aactggctg tttttgagc 
      gggaagttg gtggaagac ggggttttg accggaaca 
      cagtgcaaa cacccgggg gtgcaagcg gttttaaaa 
      cttgacccc agtaacaac aagtctgat cactattcc 
      ttgctgtgg tccgatctg gggaacaaa aactgccaa 
      ggagttgtc cgaattttc aagcacgag gaccgggac 
      aaggcgttg catttgtgc agttggcat tttgagaga 
      gattcaaac cagctgacc gcctttttg gagcagctt 
      gaaaaagaa ggggcgcac acaagagct gcagcgatc 
      gcggttttt agtttaaaa atccggctc gcgattgaa 
      attttgagt cgggctccg gaaaccctg gccttcggg 
      ttggggcta aatgttgta gcaatggca cttgcgggg 
      ttcaccgac gataagaat agtgtctgg aaacagttc 
      tgttcgacg gcgaagcaa aaactgtcc gatccgtat 
      ttgaaagtg atttttagt ttcttgact gctgagaat 
      tacaatttc gagagcgtt ttgaacgag agtggggta 
      acagtcgac gatcgagtc gcttttgct tgcatgttt 
      ttgcccgac aaccgactg cacgactat ttaaagaaa 
      ttgtcagat aagttgata gaagagggg aatttggac 
      ggtttattg ctcacagga acaaaccag gacgggatt 
      aagttgttg caaagatat ttggacgcc actggtgat 
      atccaatcg actgctctc atagccgtc cgtgctttt 
      cccgtggaa caattctcc gaagttatc aaagtctgg 
      atctcgagt taccgcaac ctgcttgat acttggcag 
      ttgtggaac gaacgagcc cactttgac atcatgttg 
      agtacttac tgccccgcc gacaagccc ccacagcaa 
      gtgtacgtt tcgtgcaat tactgcggc aagaccatc 
      tcggcctac atgtacggg ctcaccagg gggcggggc 
      cccttcact cgcattggc ggcaccgcc aacaaactc 
      aagatgtcg tcatgcccc ggttgtcgg aaaccgctg 
      ccgcggtgt gcgatatgt ctcatgcat atgggcacc 
      accacaggg aatttcagc aacgaggtc gatagtaat 
      aaaattatt gaattttcc aactggttc acgtggtgt 
      cagacgtgc aggcatggc gggcacgtg tctcacatg 
      acccactgg tttgacgag cattcggaa tgtcctgtg 
      accgcctgc acttgcaaa tgcttttct gttgattcg 
      ctcagcaat gcgattatt aagtgaatt gtgatattt 
      tagctaaga tttacggta ctttaagaa tttgatttt 
      tgttaaata tatacaccc aaccaacgt ttttgtgaa 
      taaacgtgt tttcgaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY