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Gene information
General information
TC012241 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 77906..78983
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012241
    iBeetle screen iB_05196

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012241 RA
      MLCALLIVL LTVKIGEAC PDRWAKVDA WLHFNTEDK 
      GEHITGKSI NELDEYEMI EVTKANIST LCDGAIKNL 
      PELRFLSLV EANISKIQP DAFQNLPLL THLSLAVNR 
      LTSIERYQF KNLDKLNTL YLSKNRIEN VEEDAFTDL 
      IHLRKVFLD RNKLHEIDS KWFLSNPKL GLIDLSFND 
      ISKVQAGAF ALPRNSIVE IRLQNNQVE EIEPDAVTS 
      EGKDDGKPH LTLRLERNK LAKISEKFV QSMDKLRSS 
      LYFDSNQLK CFDPKTLRV LKNVTVELH VDKNPIDCD 
      CLIAATNFV EDHVLSATF YFSSTECKL PVAKPKHQP 
      QNMFFYT
    CDS sequence
    • >TC012241 RA
      atgttgtgc gcactcctg atcgtacta ctaacagtg 
      aaaattggt gaagcctgc cctgaccgc tgggccaaa 
      gtggacgcc tggctccac ttcaacacc gaagacaaa 
      ggcgagcac atcaccggc aaaagcatc aacgaatta 
      gacgagtac gaaatgatc gaagtgaca aaagccaac 
      atttccacc ttgtgcgac ggtgcgata aaaaacctc 
      cccgagctg aggtttcta agtctggta gaggccaac 
      atttcaaaa atccagcca gacgccttc cagaacttg 
      ccattactg acccactta tcactcgct gtgaatcgc 
      ctaaccagc atcgagcgc taccagttt aaaaattta 
      gacaaactc aacaccctg tatttgtcc aaaaaccga 
      atcgaaaac gtggaagag gacgccttc actgacttg 
      atccacttg cggaaggtg ttcctggac aggaacaaa 
      ctccacgag attgactcg aagtggttt ttgagcaac 
      ccgaagcta ggtttgatc gacttatct ttcaacgac 
      atcagcaag gtccaggcg ggggctttc gccttgccc 
      aggaacagt attgtggag atccgactg cagaacaac 
      caagtggag gaaattgaa ccggacgct gtgaccagc 
      gagggaaaa gacgacgga aaaccgcac ctaacgctg 
      cgcttggag cgcaacaaa ctggccaaa atttcggaa 
      aagtttgtg caaagcatg gacaagctc cgcagcagt 
      ttgtacttt gactcgaac caactcaag tgttttgac 
      cccaaaacg ctccgagtt ttgaaaaac gtcacagtt 
      gagctgcac gtcgacaaa aacccgatc gattgtgat 
      tgtttaatt gcggctacg aattttgtg gaggaccac 
      gttttgtct gccacgttc tatttctcc tcaaccgag 
      tgcaaactg cccgtggcc aagcccaag caccagccg 
      caaaatatg tttttctac acttag
    mRNA sequence
    • >TC012241 RA
      gtgtgttag taaaaccaa accgaaaat gttgtgcgc 
      actcctgat cgtactact aacagtgaa aattggtga 
      agcctgccc tgaccgctg ggccaaagt ggacgcctg 
      gctccactt caacaccga agacaaagg cgagcacat 
      caccggcaa aagcatcaa cgaattaga cgagtacga 
      aatgatcga agtgacaaa agccaacat ttccacctt 
      gtgcgacgg tgcgataaa aaacctccc cgagctgag 
      gtttctaag tctggtaga ggccaacat ttcaaaaat 
      ccagccaga cgccttcca gaacttgcc attactgac 
      ccacttatc actcgctgt gaatcgcct aaccagcat 
      cgagcgcta ccagtttaa aaatttaga caaactcaa 
      caccctgta tttgtccaa aaaccgaat cgaaaacgt 
      ggaagagga cgccttcac tgacttgat ccacttgcg 
      gaaggtgtt cctggacag gaacaaact ccacgagat 
      tgactcgaa gtggttttt gagcaaccc gaagctagg 
      tttgatcga cttatcttt caacgacat cagcaaggt 
      ccaggcggg ggctttcgc cttgcccag gaacagtat 
      tgtggagat ccgactgca gaacaacca agtggagga 
      aattgaacc ggacgctgt gaccagcga gggaaaaga 
      cgacggaaa accgcacct aacgctgcg cttggagcg 
      caacaaact ggccaaaat ttcggaaaa gtttgtgca 
      aagcatgga caagctccg cagcagttt gtactttga 
      ctcgaacca actcaagtg ttttgaccc caaaacgct 
      ccgagtttt gaaaaacgt cacagttga gctgcacgt 
      cgacaaaaa cccgatcga ttgtgattg tttaattgc 
      ggctacgaa ttttgtgga ggaccacgt tttgtctgc 
      cacgttcta tttctcctc aaccgagtg caaactgcc 
      cgtggccaa gcccaagca ccagccgca aaatatgtt 
      tttctacac ttaggcact ggatgtatg attctgatt 
      gctgatagt gacataata aagataaat atttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY