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Gene information
General information
TC012228 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 34965..36534
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012228
    iBeetle screen iB_05193

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012228 RA
      MAFLHRINH VYLVIFAAD IIAFITGTS VAWSSPVIP 
      ILENKGISK NPLGQGITD EEESWIGSL AAMGGIFGP 
      FIFGYLVQS TGRKITVTL LSIPYLIAY LLAAFADSV 
      YLYYVSRIL MGFGVGGMF CILPIFVVE SVEAKNRGA 
      LQATTTSAI MLGLLFSYS VGPYVPIRT FNLILAAFC 
      VIYVPVFWL VAPETPYYL CSVSQEDEA FKSLIYLRQ 
      KPETEVRSE LEGIKNHVK QLKPTSFCE IFRTRGTTK 
      AFVYSLVLT TAQQFSGVT VILYFTENI FHEAGSDIA 
      PEVCSIIVG AVQFVVSTI SPPFLDRVG KKVLLLVAL 
      AGAIACEVV LGVYFYLQK SGDDVSGIN WLPILSLVA 
      FIAFYNFGL GAIPWAVMG ELLPLNIIS KASVVVTSF 
      YWLVGFFLT KYFGSLSHE IGMAGSFWI FGGICVLFE 
      LFVYFFMFE TKGKSLNEI QAILNA
    CDS sequence
    • >TC012228 RA
      atggccttc ctccaccga atcaaccat gtctacctc 
      gtcatcttc gcagccgac attatcgca tttatcacc 
      gggacatcc gtagcctgg tcatcaccc gtaatacct 
      attttggaa aataaaggc atcagtaag aatcctcta 
      ggacaagga ataaccgac gaggaggag tcctggatt 
      ggctcactg gccgccatg ggtggaatt ttcggcccg 
      tttattttc ggctattta gtccaatca acaggacga 
      aaaataaca gtgacgctg ctttcaatc ccttacttg 
      atagcctat ttactggcg gctttcgcc gattcagtc 
      tacctctac tacgtcagc cgaattttg atgggcttc 
      ggagtcggt ggaatgttt tgcattctc ccgattttc 
      gtggtagaa agtgtcgaa gcgaaaaat cggggcgcc 
      ctccaagcc accaccaca agtgccata atgctgggc 
      ttgcttttc tcgtacagt gtgggcccc tatgtgccc 
      ataaggact tttaactta atccttgcg gctttctgt 
      gtcatctac gtgccggtt ttttggctc gtggcaccg 
      gaaaccccg tactatttg tgcagcgta agccaggag 
      gatgaagcg ttcaagtct ctaatctac ttgagacaa 
      aaaccggaa actgaggtc cggtcggag ttggaaggg 
      ataaaaaat cacgtcaaa cagttaaaa ccaacgtca 
      ttttgcgaa atttttcgc acaagagga acgaccaaa 
      gcttttgtg tacagtttg gtcttaaca actgcgcag 
      cagttttcc ggagtcacc gtcattctg tacttcacc 
      gagaacatt tttcacgaa gctgggagt gacatcgct 
      ccggaggtg tgttcgata attgtgggc gctgtccag 
      tttgtggtt tccacaatt tcgcccccg tttttggac 
      agagtgggc aagaaagtg ttgcttttg gtcgccctt 
      gcgggggcg attgcttgc gaagtcgtg ctgggcgtt 
      tatttttat ttgcaaaag agtggagat gatgtgtcg 
      ggtattaac tggctgcct atcttatca ctagttgcg 
      tttatcgcg ttttataat ttcggcttg ggggcgatt 
      ccgtgggcc gttatgggc gagcttttg cccctaaat 
      atcatatcg aaggcgtcc gttgtggtt acgagtttt 
      tactggctt gttggattt tttctgaca aagtatttc 
      ggttcgttg tcgcacgag atagggatg gctgggtcc 
      ttctggata tttgggggg atttgtgtg ttgtttgaa 
      ctgtttgtt tattttttc atgtttgag acgaagggg 
      aagagtctg aacgagatt caagccatt ttgaatgca 
      taa
    mRNA sequence
    • >TC012228 RA
      catactgtt taattatcg gtaacaaaa ctaataaaa 
      tttctagca ctcagtaac agtaactaa aacaactcc 
      gcaataaat tagcaccaa aatggcctt cctccaccg 
      aatcaacca tgtctacct cgtcatctt cgcagccga 
      cattatcgc atttatcac cgggacatc cgtagcctg 
      gtcatcacc cgtaatacc tattttgga aaataaagg 
      catcagtaa gaatcctct aggacaagg aataaccga 
      cgaggagga gtcctggat tggctcact ggccgccat 
      gggtggaat tttcggccc gtttatttt cggctattt 
      agtccaatc aacaggacg aaaaataac agtgacgct 
      gctttcaat cccttactt gatagccta tttactggc 
      ggctttcgc cgattcagt ctacctcta ctacgtcag 
      ccgaatttt gatgggctt cggagtcgg tggaatgtt 
      ttgcattct cccgatttt cgtggtaga aagtgtcga 
      agcgaaaaa tcggggcgc cctccaagc caccaccac 
      aagtgccat aatgctggg cttgctttt ctcgtacag 
      tgtgggccc ctatgtgcc cataaggac ttttaactt 
      aatccttgc ggctttctg tgtcatcta cgtgccggt 
      tttttggct cgtggcacc ggaaacccc gtactattt 
      gtgcagcgt aagccagga ggatgaagc gttcaagtc 
      tctaatcta cttgagaca aaaaccgga aactgaggt 
      ccggtcgga gttggaagg gataaaaaa tcacgtcaa 
      acagttaaa accaacgtc attttgcga aatttttcg 
      cacaagagg aacgaccaa agcttttgt gtacagttt 
      ggtcttaac aactgcgca gcagttttc cggagtcac 
      cgtcattct gtacttcac cgagaacat ttttcacga 
      agctgggag tgacatcgc tccggaggt gtgttcgat 
      aattgtggg cgctgtcca gtttgtggt ttccacaat 
      ttcgccccc gtttttgga cagagtggg caagaaagt 
      gttgctttt ggtcgccct tgcgggggc gattgcttg 
      cgaagtcgt gctgggcgt ttattttta tttgcaaaa 
      gagtggaga tgatgtgtc gggtattaa ctggctgcc 
      tatcttatc actagttgc gtttatcgc gttttataa 
      tttcggctt gggggcgat tccgtgggc cgttatggg 
      cgagctttt gcccctaaa tatcatatc gaaggcgtc 
      cgttgtggt tacgagttt ttactggct tgttggatt 
      ttttctgac aaagtattt cggttcgtt gtcgcacga 
      gatagggat ggctgggtc cttctggat atttggggg 
      gatttgtgt gttgtttga actgtttgt ttatttttt 
      catgtttga gacgaaggg gaagagtct gaacgagat 
      tcaagccat tttgaatgc ataaataag ccaaataaa 
      tatttttgt atttttagt acgaaataa atttaataa 
      aaaatttca gataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY