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Gene information
General information
TC012322 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4151038..4240972
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012322
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05191

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    leg musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    labium proximal decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    maxilla orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs coxa size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs trochanter size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 9 (16 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 9

    head orientation irregular - Number of animals showing the phenotype: 9

    elytra blistered - Number of animals showing the phenotype: 9

    legs orientation irregular - Number of animals showing the phenotype: 9

    legs femur shape irregular -

    abdomen gin-traps shortened - Number of animals showing the phenotype: 9

    female genital lobes rounded - Number of animals showing the phenotype: 9

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012322 RA
      MPRRKQDCP KRMKWEGGE EGSPEEGDK LEGDEDTGS 
      MTPNSAASP APPEEEAAS PTPGDVPTP SSPRSISED 
      PLNCRDLPN SRCNSRESS DSLVQPRCP SGESMLSER 
      AALLRGVPT LSPVGVALP PTLPAAAAA ALLPPQTAA 
      MAAYLNAAA VAAAAQQSH RLMMTSPSG FNRASVSPL 
      ISSSSSPPG VFPVDAPMH SPPGDGILD FSKRGQKSD 
      ADSDSDVVN LSKPGTPPS GEPGNGPLD LSVSSRKRS 
      NDDSVSQPP NRKPRTDFK PQVLPQWPS PIGASHLPY 
      FAAAVAAAS NLSPKTNSS PDLWNGKLK HGGPTPSDA 
      TKALEKMSE LSKLGGEDL FRTMANAAP GNSASNRHS 
      AWQSHWLNK GAEQAKDVL KCVWCKQSF PSLAAMTTH 
      MKEAKHCGV NVPVPPMQS QIINPQPQI TSPSNTNTS 
      TSSTNSNKP NSSDLNMLI KETMPLPRK LVRGQDVWL 
      GKGAEQTRQ ILKCMWCGQ SFRSLAEMT SHMQQTQHY 
      TNIISQEQI ISWRSSDDA KGGGGGSGS GNNQPPGGP 
      SSHVSAVLT CKVCDQAFS SLKELSNHM VKNSHYKEH 
      IMRSITESG GRRRQTREK RKKSLPVRK LLELERAQH 
      EFKNGDSSS LLDKMRDSS GAGRITCEK CGNKIDTSL 
      FVDHIRQCV GGGTIGSNQ RNFLKNALM SNNILPPES 
      PTRDKSKQN EKSPSPQQR SPSVTDLST KDGTTVPES 
      NNGSSPSVL NAIEKLIEK SFDSRSRQN SSFPGHGPT 
      TAPMGSSIL KRLGIDESV DYTKPLVDA QTMNLLRSY 
      HQQQSHHYG RRERSGSES SSISERCSS RVESLTPER 
      KMEPPGVPL NSTPRSTPD KREKSPTSD KAQEADGEV 
      VVKKEVDEE ERLENHTSV KIKREAEDR DEDERSFHS 
      NGAKEEDED KPSVSPLTS PRQPAETHA GSPCRNSAS 
      PASSDRSGT PRSTNGDRK PGGSLGALS SMFDSLSGN 
      NTGDALAPS GGKKGSSHP LAALQKLCD KTETHTNNR 
      THSATSNSS VNANTNNIP TNAGTTPGA ILAFSWACN 
      DAVMTSDSI MKCAFCDTP FISKGAYRH HLSKMHFVK 
      DGVIPDPVA LKSAQQGSS SSSEGVPLK GTAVPPPSG 
      SSGPGSTGS KSPPVAGFE ESPHSKFLK YTELAKQLS 
      SKYV
    CDS sequence
    • >TC012322 RA
      atgccgaga cgaaaacag gattgccct aaaaggatg 
      aaatgggaa ggtggcgag gaaggctcg ccggaagaa 
      ggcgataaa ttggagggc gacgaagac accggctcc 
      atgacgccc aactcggcc gcctctccc gcacctcct 
      gaagaagag gccgcttcg ccgactccc ggcgatgtc 
      cccactccc tctagtcca cgctccatc agcgaagat 
      cctctcaac tgcagggat ttaccgaat tcccggtgc 
      aattctcgc gagtcatcg gattctcta gtgcagccc 
      cgctgtcca tcgggcgag tcgatgctt tcggagcgt 
      gcagccttg cttcgaggc gtcccaacc ctgagcccc 
      gtcggggtg gcccttccg ccgaccctt ccagcggcg 
      gcggcggcg gctttgctg ccgcctcag accgccgcc 
      atggcggcc tacctcaac gcggcggcc gtggcggcg 
      gcggcccaa cagtcccac cgcctcatg atgacctcg 
      ccgtccggc ttcaaccgg gcctcggtc tcgccgctg 
      atctcatcc tcgtcgtcg cctccaggg gtcttcccc 
      gttgacgcg ccgatgcac tctccgccc ggtgacggc 
      atcttagac tttagtaaa cgtggccaa aaaagtgac 
      gctgatagt gactctgac gtggttaat ctcagtaaa 
      ccggggact cccccgagc ggcgagccc ggaaacggc 
      cctcttgac cttagtgtg agcagccgg aagcgctct 
      aacgacgac tccgtgtcg cagccgccg aatcgcaaa 
      ccgagaact gacttcaaa ccgcaggtt ctgccccag 
      tggccctcg ccgatcggt gcttcgcac ttgccgtat 
      tttgcggcc gccgtcgcc gccgcgtcg aatctttcg 
      ccaaaaacg aacagttcg cccgacttg tggaacggc 
      aaattgaaa cacgggggc cccaccccc agcgacgcc 
      accaaagcg ctcgaaaaa atgagcgag ttgagcaaa 
      ctcggcggc gaggactta ttccgtact atggcgaac 
      gccgcccca ggaaactcc gccagtaac aggcacagc 
      gcttggcag tcgcactgg ttaaacaag ggcgccgag 
      caagcaaaa gacgtgctg aagtgcgtc tggtgcaag 
      cagagcttc cccagttta gccgcgatg acgacgcac 
      atgaaggaa gcgaaacac tgcggcgtc aacgttccc 
      gtccctcca atgcaatca caaatcatt aatcctcaa 
      cctcaaata acatcgcct tcgaacaca aacacaagt 
      acctcttcg actaactcg aacaaaccg aattcatcc 
      gatctcaac atgttgatc aaagaaacg atgcctctg 
      ccacgaaag ttagttagg ggacaagac gtgtggctg 
      ggcaaggga gccgaacaa acaagacaa atactcaag 
      tgtatgtgg tgcggacaa agtttcaga tctttggcc 
      gaaatgacc agtcacatg caacaaacg cagcattac 
      acaaacatc atctcgcaa gaacagatc atttcgtgg 
      cggtcgtcg gacgacgcg aaagggggc ggcggaggg 
      agcggctca ggtaacaac caaccaccg gggggaccc 
      agcagtcat gtcagcgcg gtcctcact tgcaaagtg 
      tgcgaccaa gctttcagt tctctgaaa gagttgagt 
      aaccacatg gtcaaaaat tcgcactat aaagaacac 
      ataatgaga tcgatcact gaaagtggg ggtcgccga 
      aggcagacg agggagaaa cgcaaaaaa tcactcccc 
      gtacgaaaa ctactagaa ctagaaaga gcccaacac 
      gaattcaag aacggtgat agtagcagt ttactcgat 
      aaaatgcgc gattcttca ggagccggc aggattact 
      tgtgaaaag tgtggcaat aaaattgac acgtctttg 
      ttcgttgat catataaga cagtgtgtc gggggcggt 
      accatagga agcaatcaa agaaatttc ctcaaaaac 
      gcactcatg tcaaataat attttacca cctgaaagt 
      ccaactaga gataagtcc aagcaaaac gaaaagtcc 
      ccttcaccc caacaacgg tcaccttca gtcactgac 
      ttaagtacc aaggatggt acgactgtt ccggagagt 
      aataacgga tcttcccct tctgttctc aacgctatt 
      gagaaacta attgaaaaa agtttcgac tcaagatct 
      cggcaaaac tcgtctttt cccggtcat ggtccaact 
      acagctccc atgggttct agtatttta aaacgacta 
      ggaattgac gaaagcgtc gactacacc aaaccctta 
      gtagacgct caaaccatg aacttacta cgaagttat 
      caccagcaa caaagccat cattacggt agacgggaa 
      agaagcggg agcgaatcg agttcgata tcggagcgg 
      tgtagtagt cgagtagag tcgttaaca ccggaacgc 
      aaaatggag ccgccggga gtacccctg aacagcacc 
      cccaggtcc acccccgac aagcgggag aaatcgcca 
      acttccgac aaagcccaa gaggcggac ggcgaagtc 
      gtagtaaaa aaagaagta gacgaggag gaacgtctc 
      gagaatcac actagtgtt aaaattaaa cgtgaagct 
      gaagaccgc gacgaagac gagcgcagt tttcacagt 
      aacggtgcg aaggaggag gacgaggac aagccctcg 
      gtgagtccg ctgaccagc ccgcggcag cccgcggag 
      acccacgcg ggcagcccc tgtcggaac agcgccagt 
      cccgccagc agcgaccgc tcgggcact ccgcggagc 
      accaacggc gaccggaag ccgggcggc agcctgggg 
      gcgctgagt tcgatgttc gacagcctg tccggcaac 
      aacaccggt gacgcatta gcgccgtca ggtggcaaa 
      aagggctcg agtcatcca ttagcagcc ttacaaaaa 
      ttatgtgat aagactgaa acccacaca aacaaccgc 
      acacactct gcgacatcc aattcgagc gtcaacgcc 
      aacacgaac aatatccca acgaacgcc gggacgact 
      cccggggcg attttggcc ttcagctgg gcctgcaac 
      gacgcggtg atgacctcg gattcgata atgaagtgc 
      gcgttttgc gacacgccg ttcatctcg aagggggcg 
      tacagacac cacttgtcg aaaatgcac ttcgtgaag 
      gacggggtg atccccgac ccggtggcg ctcaagtcg 
      gcgcagcag ggctcgagc tcgtccagc gagggggtg 
      ccgctgaag ggcaccgcc gtgccgccc ccaagcggc 
      tccagcggc ccgggctcg accgggagc aagagcccg 
      ccggtggcg gggttcgag gaaagcccg cattcgaag 
      ttcttgaaa tatacggag ttagctaag cagttgtct 
      agcaagtat gtgtaa
    mRNA sequence
    • >TC012322 RA
      gctagtgac gagagatga catcctgaa aagcagaca 
      gagagcgaa gagcgacgc cagagacgc cggcgccta 
      caacctgac gaaacgttg cgccgattg tcgtttgtt 
      cgctggcgg tagtggggg aaaaaccgc gctccgtgt 
      tgtcagtca ctcagtcaa cggaggctc tgcatctgg 
      gaaggacgc tcttcactt gttccgtgc gttgacatt 
      cgcgccaac tgtcgcgag ggccgaatt gacagtgac 
      cggtgacgc agtgataga ccgcgagcc tcgcgaccg 
      ccgaaccat gctcgccca ccatcccct acttgcgta 
      tacgagata ccagtgctg tgcagtgct cctccgcta 
      agcgagtcc gctttttgg gttccggat gttagtgat 
      gtttgtttg gtggagaaa atgaactag agaagagcg 
      aaaagtcgg ccaagattt tgcgaacgt cgtccagga 
      gcgcggcca aaatgccga gacgaaaac aggattgcc 
      ctaaaagga tgaaatggg aaggtggcg aggaaggct 
      cgccggaag aaggcgata aattggagg gcgacgaag 
      acaccggct ccatgacgc ccaactcgg ccgcctctc 
      ccgcacctc ctgaagaag aggccgctt cgccgactc 
      ccggcgatg tccccactc cctctagtc cacgctcca 
      tcagcgaag atcctctca actgcaggg atttaccga 
      attcccggt gcaattctc gcgagtcat cggattctc 
      tagtgcagc cccgctgtc catcgggcg agtcgatgc 
      tttcggagc gtgcagcct tgcttcgag gcgtcccaa 
      ccctgagcc ccgtcgggg tggcccttc cgccgaccc 
      ttccagcgg cggcggcgg cggctttgc tgccgcctc 
      agaccgccg ccatggcgg cctacctca acgcggcgg 
      ccgtggcgg cggcggccc aacagtccc accgcctca 
      tgatgacct cgccgtccg gcttcaacc gggcctcgg 
      tctcgccgc tgatctcat cctcgtcgt cgcctccag 
      gggtcttcc ccgttgacg cgccgatgc actctccgc 
      ccggtgacg gcatcttag actttagta aacgtggcc 
      aaaaaagtg acgctgata gtgactctg acgtggtta 
      atctcagta aaccgggga ctcccccga gcggcgagc 
      ccggaaacg gccctcttg accttagtg tgagcagcc 
      ggaagcgct ctaacgacg actccgtgt cgcagccgc 
      cgaatcgca aaccgagaa ctgacttca aaccgcagg 
      ttctgcccc agtggccct cgccgatcg gtgcttcgc 
      acttgccgt attttgcgg ccgccgtcg ccgccgcgt 
      cgaatcttt cgccaaaaa cgaacagtt cgcccgact 
      tgtggaacg gcaaattga aacacgggg gccccaccc 
      ccagcgacg ccaccaaag cgctcgaaa aaatgagcg 
      agttgagca aactcggcg gcgaggact tattccgta 
      ctatggcga acgccgccc caggaaact ccgccagta 
      acaggcaca gcgcttggc agtcgcact ggttaaaca 
      agggcgccg agcaagcaa aagacgtgc tgaagtgcg 
      tctggtgca agcagagct tccccagtt tagccgcga 
      tgacgacgc acatgaagg aagcgaaac actgcggcg 
      tcaacgttc ccgtccctc caatgcaat cacaaatca 
      ttaatcctc aacctcaaa taacatcgc cttcgaaca 
      caaacacaa gtacctctt cgactaact cgaacaaac 
      cgaattcat ccgatctca acatgttga tcaaagaaa 
      cgatgcctc tgccacgaa agttagtta ggggacaag 
      acgtgtggc tgggcaagg gagccgaac aaacaagac 
      aaatactca agtgtatgt ggtgcggac aaagtttca 
      gatctttgg ccgaaatga ccagtcaca tgcaacaaa 
      cgcagcatt acacaaaca tcatctcgc aagaacaga 
      tcatttcgt ggcggtcgt cggacgacg cgaaagggg 
      gcggcggag ggagcggct caggtaaca accaaccac 
      cggggggac ccagcagtc atgtcagcg cggtcctca 
      cttgcaaag tgtgcgacc aagctttca gttctctga 
      aagagttga gtaaccaca tggtcaaaa attcgcact 
      ataaagaac acataatga gatcgatca ctgaaagtg 
      ggggtcgcc gaaggcaga cgagggaga aacgcaaaa 
      aatcactcc ccgtacgaa aactactag aactagaaa 
      gagcccaac acgaattca agaacggtg atagtagca 
      gtttactcg ataaaatgc gcgattctt caggagccg 
      gcaggatta cttgtgaaa agtgtggca ataaaattg 
      acacgtctt tgttcgttg atcatataa gacagtgtg 
      tcgggggcg gtaccatag gaagcaatc aaagaaatt 
      tcctcaaaa acgcactca tgtcaaata atattttac 
      cacctgaaa gtccaacta gagataagt ccaagcaaa 
      acgaaaagt ccccttcac cccaacaac ggtcacctt 
      cagtcactg acttaagta ccaaggatg gtacgactg 
      ttccggaga gtaataacg gatcttccc cttctgttc 
      tcaacgcta ttgagaaac taattgaaa aaagtttcg 
      actcaagat ctcggcaaa actcgtctt ttcccggtc 
      atggtccaa ctacagctc ccatgggtt ctagtattt 
      taaaacgac taggaattg acgaaagcg tcgactaca 
      ccaaaccct tagtagacg ctcaaacca tgaacttac 
      tacgaagtt atcaccagc aacaaagcc atcattacg 
      gtagacggg aaagaagcg ggagcgaat cgagttcga 
      tatcggagc ggtgtagta gtcgagtag agtcgttaa 
      caccggaac gcaaaatgg agccgccgg gagtacccc 
      tgaacagca cccccaggt ccacccccg acaagcggg 
      agaaatcgc caacttccg acaaagccc aagaggcgg 
      acggcgaag tcgtagtaa aaaaagaag tagacgagg 
      aggaacgtc tcgagaatc acactagtg ttaaaatta 
      aacgtgaag ctgaagacc gcgacgaag acgagcgca 
      gttttcaca gtaacggtg cgaaggagg aggacgagg 
      acaagccct cggtgagtc cgctgacca gcccgcggc 
      agcccgcgg agacccacg cgggcagcc cctgtcgga 
      acagcgcca gtcccgcca gcagcgacc gctcgggca 
      ctccgcgga gcaccaacg gcgaccgga agccgggcg 
      gcagcctgg gggcgctga gttcgatgt tcgacagcc 
      tgtccggca acaacaccg gtgacgcat tagcgccgt 
      caggtggca aaaagggct cgagtcatc cattagcag 
      ccttacaaa aattatgtg ataagactg aaacccaca 
      caaacaacc gcacacact ctgcgacat ccaattcga 
      gcgtcaacg ccaacacga acaatatcc caacgaacg 
      ccgggacga ctcccgggg cgattttgg ccttcagct 
      gggcctgca acgacgcgg tgatgacct cggattcga 
      taatgaagt gcgcgtttt gcgacacgc cgttcatct 
      cgaaggggg cgtacagac accacttgt cgaaaatgc 
      acttcgtga aggacgggg tgatccccg acccggtgg 
      cgctcaagt cggcgcagc agggctcga gctcgtcca 
      gcgaggggg tgccgctga agggcaccg ccgtgccgc 
      ccccaagcg gctccagcg gcccgggct cgaccggga 
      gcaagagcc cgccggtgg cggggttcg aggaaagcc 
      cgcattcga agttcttga aatatacgg agttagcta 
      agcagttgt ctagcaagt atgtgtaa
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