| General information | |||
| TC012189 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 3646522..3654836 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC012189 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05190 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 50
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC012189 RA
MNLIFNFKA VKSCGIGFQ TFKKRSGTT DLGKEYEHL VISYLILKL IKNNQIENF FISSNDSSY GAFDDVVLE VRYTGEKQY ETYALQLKH KENGTLKIQ VLTQAKGNF SIGKYFEDY ETNLSKRSA KVVLFTNLK FQNGSGQLT LNLKNEEKK YHITEVQQG KLNLMTTSE GKVYKIDSE EAIFFEKFY LYTDQRNVD DLKKKCLSE FDNIFSSGK TGFNEYLTF ITNWSLKVG RKKEKLSKT WIQEVLSML VLTPLIQPL VFVSEATSE NAKLFQDAV SKFQITILN ESSYEKVKS VWKNATQNI DFKELNKIN TKYQVMADN ILNTEELVK KNGTEVNTI LWLMGKCPL IVKNDKKIT KVIDLLSDE QKLIILHSG QKGKTDLQH QNCFQNLGN IKSEEKLYD DILQNFTFA IEGQTPALL KDLVRICKE VEHTVTTDD LLQMMEGNF EIGKSEELS LPPYYVPRA MSRVLINSE YLNTFSNDT LVIISCVTN LNTFKKRFE EYKIVDIDE HFKNEDVKK KNKERKTIY ITRNKCVQE QFDTLCSRY PEKRNCHLM KYHNLYSLE WVRSSNGVK DLQKFKMSK EDQDIKEVQ LLATFFKDN FNIQKTANV DHFLEIDRI RDFGSKTLS DFRLNKTNS IEELEFFRN SHDNLINVV CENPGMGKT TLMKSLKKN TFSCLWTIF IVAGNHAKH FRDNPKGDV GKFLDYITQ ANYRNNETE MQILQNFVS NKQIVLVWD GLDEVSDKT LEIIKRIVH GVSRKKITQ WITSRINLE DVMESEFGV FCRRIEQFK KEELQSFIK NRLTCSDDE LLNIFDEIK LNIQLVRYN SILEIPLHI YILTELLIS DKETTLTLL GNKEQIFSV SDIYQRFVD KVIEQNCKE KSNYDFEKE SIIKKFDEE KTKAINDYK KIAIYYYIT ELSNKTKCR KILKQIKEK KDPFGFIIK VTEEMSPQF SHKSFGEYF AALYLSENL EKIYKISDF IQNDKYDNI RFFLDLILA KNSKAHIAV LYKNSILLD HCSNEDLNR KDLLGRNSF ELSCEWDKR YPLLKTQKR KECYSISIT FPVTIPAKK RKKNISNFL KEYDDADFK LKKNILLFP FIVTSNTNY YTEIDKINI PSIFYYAVQ HNHALVFEY FGQVPFILI KTFIPDIIW TLLDLAIHS KAIHSINFL LRNPQYRNQ LKANVSLSY SLCTLQSRK MLKAFTDSG WNINEIHSD GMAVVHLAC ISADLLTLQ FIIENDGDV NVCNKDGRL PIHFACQYG NINVVKFLF DHGAKIDVC DQNGNRPLD LACVNEKYG CDIVKFLLE NGQNLNICN KNGSLPMHL VCLFGSISV VKFLFDHGA KIDVCDQNG DRPLHFACV NEKYGCDIV KFLLENGQS LNICNKIGS LPMHLACLF GSISVVKFL FDHGAKIDV CDQKGNRPL HFACQNEKY GCDIVKFLL ENGQSLNIC NKNGSVPMH LVCLFGSIS VVKFLFDHG AKIDVYDQN GNRPIHFAC LNEKYGCDI VKFLLGKGQ SLDVCNKNG HLPIHFAYQ NGSINVVKF LFDHGAKID VYDQEGSRP LHFACVNEK YGCDIVKFL LENGQSLNI CNKNGSVPM HLACLFGSI SVVKFLFDR GAKIDVCDQ KGNRPLHFA CQNEKYGCD IVKFLLENG QNLNICNKN GSLPMHLAC LFGSISVVK FLFDHGAKI DVYDQNGNR PIHFACQNE KYGCDIVKF LLGKGQSLD VCNKNGSLP MHLACQYAS TNVVKVLLD NSMKVNVCR QDRQAFINL ARLNEKHGY EIVKLLSNC LQ
- >TC012189 RB
MAHNSSENN NIRQNLPEP EPEPPTTTP STTFKKRSG TTDLGKEYE HLVISYLIL KLIKNNQIE NFFISSNDS SYGAFDDVV LEVRYTGEK QYETYALQL KHKENGTLK IQVLTQAKG NFSIGKYFE DYETNLSKR SAKVVLFTN LKFQNGSGQ LTLNLKNEE KKYHITEVQ QGKLNLMTT SEGKVYKID SEEAIFFEK FYLYTDQRN VDDLKKKCL SEFDNIFSS GKTGFNEYL TFITNWSLK VGRKKEKLS KTWIQEVLS MLVLTPLIQ PLVFVSEAT SENAKLFQD AVSKFQITI LNESSYEKV KSVWKNATQ NIDFKELNK INTKYQVMA DNILNTEEL VKKNGTEVN TILWLMGKC PLIVKNDKK ITKVIDLLS DEQKLIILH SGQKGKTDL QHQNCFQNL GNIKSEEKL YDDILQNFT FAIEGQTPA LLKDLVRIC KEVEHTVTT DDLLQMMEG NFEIGKSEE LSLPPYYVP RAMSRVLIN SEYLNTFSN DTLVIISCV TNLNTFKKR FEEYKIVDI DEHFKNEDV KKKNKERKT IYITRNKCV QEQFDTLCS RYPEKRNCH LMKYHNLYS LEWVRSSNG VKDLQKFKM SKEDQDIKE VQLLATFFK DNFNIQKTA NVDHFLEID RIRDFGSKT LSDFRLNKT NSIEELEFF RNSHDNLIN VVCENPGMG KTTLMKSLK KNTFSCLWT IFIVAGNHA KHFRDNPKG DVGKFLDYI TQANYRNNE TEMQILQNF VSNKQIVLV WDGLDEVSD KTLEIIKRI VHGVSRKKI TQWITSRIN LEDVMESEF GVFCRRIEQ FKKEELQSF IKNRLTCSD DELLNIFDE IKLNIQLVR YNSILEIPL HIYILTELL ISDKETTLT LLGNKEQIF SVSDIYQRF VDKVIEQNC KEKSNYDFE KESIIKKFD EEKTKAIND YKKIAIYYY ITELSNKTK CRKILKQIK EKKDPFGFI IKVTEEMSP QFSHKSFGE YFAALYLSE NLEKIYKIS DFIQNDKYD NIRFFLDLI LAKNSKAHI AVLYKNSIL LDHCSNEDL NRKDLLGRN SFELSCEWD KRYPLLKTQ KRKECYSIS ITFPVTIPA KKRKKNISN FLKEYDDAD FKLKKNILL FPFIVTSNT NYYTEIDKI NIPSIFYYA VQHNHALVF EYFGQVPFI LIKTFIPDI IWTLLDLAI HSKAIHSIN FLLRNPQYR NQLKANVSL SYSLCTLQS RKMLKAFTD SGWNINEIH SDGMAVVHL ACISADLLT LQFIIENDG DVNVCNKDG RLPIHFACQ YGNINVVKF LFDHGAKID VCDQNGNRP LDLACVNEK YGCDIVKFL LENGQNLNI CNKNGSLPM HLVCLFGSI SVVKFLFDH GAKIDVCDQ NGDRPLHFA CVNEKYGCD IVKFLLENG QSLNICNKI GSLPMHLAC LFGSISVVK FLFDHGAKI DVCDQKGNR PLHFACQNE KYGCDIVKF LLENGQSLN ICNKNGSVP MHLVCLFGS ISVVKFLFD HGAKIDVYD QNGNRPIHF ACLNEKYGC DIVKFLLGK GQSLDVCNK NGHLPIHFA YQNGSINVV KFLFDHGAK IDVYDQEGS RPLHFACVN EKYGCDIVK FLLENGQSL NICNKNGSV PMHLACLFG SISVVKFLF DRGAKIDVC DQKGNRPLH FACQNEKYG CDIVKFLLE NGQNLNICN KNGSLPMHL ACLFGSISV VKFLFDHGA KIDVYDQNG NRPIHFACQ NEKYGCDIV KFLLGKGQS LDVCNKNGS LPMHLACQY ASTNVVKVL LDNSMKVNV CRQDRQAFI NLARLNEKH GYEIVKLLS NCLQ
- >TC012189 RA
atgaatttg attttcaat ttcaaggcg gttaaaagt tgtggtatc ggttttcag actttcaaa aaacgaagt ggtactaca gatcttggc aaagagtac gaacacttg gtcatttct tatctaatt ctcaaatta attaaaaat aaccaaatt gaaaatttt tttatttct tccaacgat agcagttac ggtgctttc gatgatgtt gtattggaa gtgagatac acaggagaa aaacaatac gaaacgtat gctctacag ttaaaacac aaagaaaat ggcacatta aaaattcaa gttctaact caagcaaag ggtaatttc agtattgga aaatatttt gaagactat gagacaaac ttatcaaaa agatcggct aaagtggtt ttgtttacg aatcttaaa tttcaaaac ggttcagga caattgaca ttgaatttg aaaaatgag gaaaagaag tatcatatc acagaagtt caacagggc aagcttaat ttaatgaca acttcagag ggaaaggtg tacaagatt gattctgaa gaagcaatt ttttttgaa aaattttat ctatatact gaccagcga aatgttgat gatttaaaa aagaaatgt ttatcagaa tttgacaac attttttct tctggaaaa actggcttc aacgagtac ttaacattt ataaccaat tggagttta aaagttgga agaaaaaaa gaaaagttg agtaaaacc tggattcaa gaagttttg tcgatgctc gtgttaact cctctaatt cagccatta gtgttcgtc tcagaagcg acaagtgaa aatgcaaag ttatttcaa gacgctgtt tcaaagttc caaattacg attctcaac gaaagcagt tacgaaaaa gtcaaatct gtttggaaa aacgcaact caaaatatt gatttcaag gaacttaac aaaattaac acgaaatac caggtaatg gcggacaac attcttaat acagaagaa ttagtaaag aaaaacggc accgaagtt aatacgatt ctttggttg atgggcaaa tgtccccta atagtaaaa aacgacaaa aagattacc aaagttata gatttgtta agtgatgaa caaaaattg attattctt cactctgga cagaagggg aagacggat ttgcaacac caaaactgt tttcaaaat ttgggcaat attaaatcc gaagagaaa ctttatgat gatatttta caaaatttt acctttgca atagaagga caaacacct gctttatta aaagacttg gttcgcatt tgcaaagaa gtggaacat acagtaaca acggatgat cttctgcaa atgatggaa ggaaacttt gaaattgga aaatcagag gaactctca ctgccacct tactacgtg ccaagagct atgtctcga gtcttgata aattcggaa tatttgaat acattttca aacgacacc ttagttatc attagttgt gtgacaaat ttaaatacc ttcaagaag agatttgaa gaatacaag attgttgac attgatgag cattttaaa aatgaagat gtgaagaaa aagaataag gaaaggaaa actatctac atcaccagg aataagtgc gtgcaggaa caatttgat acattgtgt tcaagatat ccggaaaaa agaaactgc caccttatg aaatatcac aatttgtat tctttagaa tgggtcaga agttctaat ggagtcaag gatttgcaa aaattcaaa atgtcaaaa gaagatcaa gacatcaaa gaagttcag ttacttgca acttttttt aaagataat ttcaacatt cagaaaaca gcaaatgtt gatcacttc ctggaaatt gacagaata agggacttt ggctcaaaa accttgtca gatttccgg ctaaataaa accaattcc attgaagaa ttagaattc tttcgtaat tcacatgac aatctcatc aacgtcgta tgtgaaaat ccaggaatg ggtaaaaca acgttaatg aaaagtttg aagaagaac actttttct tgtctttgg accatcttc atagtggca ggaaaccac gctaaacac tttcgggac aatccaaaa ggagacgtt ggaaaattc ttggattat atcacgcaa gccaattat agaaataat gaaacagag atgcaaatt ttgcagaat tttgtgtct aataaacaa atagttttg gtttgggat ggtcttgat gaagtcagc gataaaact ctggagatt ataaagcgt attgtacat ggtgtttcc cgaaagaaa attacacag tggataact tctagaatt aacttagaa gatgtaatg gaaagcgaa tttggtgtt ttttgtaga cgcattgaa cagtttaag aaagaagag ttacaatct tttataaaa aatcgctta acctgttca gatgacgaa ttattaaac atttttgat gaaattaaa ttgaacatt caacttgtt cgttataac agtattttg gaaatacca ttacatata tacatttta acagaatta cttattagt gataaagaa acgactttg actttgtta ggaaacaag gagcaaata ttttcggtg agtgatata tatcagcgt tttgttgac aaagtaatt gaacaaaat tgtaaagaa aaatctaac tacgatttt gaaaaggaa tccattatt aaaaaattt gatgaagaa aaaacaaaa gcaattaac gactataag aaaatagcc atctattat tatataaca gaattatcc aacaaaact aaatgtaga aaaattcta aaacaaata aaagaaaaa aaagatcct ttcggtttt ataataaaa gtcacagag gagatgtcg ccacagttt tcgcataaa tctttcggg gagtatttt gcagcttta tatctttct gaaaatctt gaaaaaatt tacaaaatt tcagatttt atacagaat gacaaatac gataacatt cgatttttc ctagacttg attttggca aaaaattca aaagctcat attgcagtg ttgtataaa aatagtata ttattagat cactgtagt aatgaagat ttaaatcgt aaagattta cttgggcgt aatagtttt gaactgtca tgtgagtgg gataagcga tatcctctt ttaaaaacg caaaaacgc aaagaatgt tattctatt tccattaca tttccagta actattcct gcaaaaaaa agaaaaaaa aacatttca aattttcta aaagaatat gatgatgca gattttaaa ttaaaaaaa aatattctt ttgtttcct tttatagta acaagtaat acaaattat tataccgaa attgacaaa attaatatt ccttcaatt ttttattat gcagtccaa cataatcac gcacttgtc ttcgaatac tttggacaa gttccattt attctcata aaaacgttt attcctgat atcatttgg acactttta gacctagca atccactca aaggcaatt cattcgata aatttcctt ttgcgtaat ccacagtat cgaaatcag ttaaaggcg aatgtaagt ctttcatac agcttgtgt accttacag tcacgcaaa atgttaaag gctttcact gacagtgga tggaatata aatgaaata catagcgat ggtatggct gttgtacat ctagcttgt attagcgca gacttatta acacttcaa tttataatt gaaaatgac ggagatgta aatgtttgc aataaagat ggtcgtttg cccatacat tttgcatgt caatatggc aacataaat gtcgtaaaa tttcttttt gatcatggt gcaaagata gatgtttgt gatcaaaat ggcaatcgg ccattagat ttggcatgt gtgaatgaa aagtatggt tgcgacatt gtaaaattt cttttagaa aacggtcaa aatttgaat atttgcaat aaaaatggt agcttgcct atgcatttg gtatgtcta tttggtagc ataagcgtc gtaaaattt ctttttgat catggtgca aagatagat gtttgtgat caaaatggc gatcggcca ttacatttc gcatgtgtg aatgaaaag tatggttgc gacattgta aaatttctt ttagaaaac ggtcaaagt ttgaatatt tgcaataaa attggtagc ttgcctatg catttggca tgtctattt ggtagcata agcgtcgta aaatttctt tttgatcat ggtgcaaaa atagatgtt tgtgatcaa aagggcaat cggccatta cattttgca tgtcagaat gaaaagtat ggttgcgac attgtaaaa tttctttta gaaaacggt caaagtttg aatatttgc aataaaaat ggtagcgtg cctatgcat ttggtatgt ctatttggt agcataagc gtcgtaaaa tttcttttt gatcatggt gcaaagata gatgtttac gatcaaaat ggcaatcgg ccgatacat ttcgcatgt ctgaatgaa aagtatggt tgcgacatt gtaaaattt cttttagga aagggtcaa agtttggat gtttgcaat aaaaatggt catttgcct atacatttt gcatatcaa aatggtagc ataaatgtc gtaaaattt ctttttgat catggtgca aagatagat gtttacgat caagagggc agtcggcca ttacatttc gcatgtgtg aatgaaaag tatggttgc gacattgta aaatttctt ttagaaaac ggtcaaagt ttgaatatt tgcaataaa aatggtagc gtgcctatg catttggca tgtctattt ggtagcata agcgtcgta aaatttctt tttgatcgt ggtgcaaag atagatgtt tgtgatcaa aagggcaat cggccatta cattttgca tgtcagaat gaaaagtat ggttgcgac attgtaaaa tttctttta gaaaacggt caaaatttg aatatttgc aataaaaat ggtagcttg cctatgcat ttggcatgt ctatttggt agcataagc gtcgtaaaa tttcttttt gatcatggt gcaaagata gatgtttac gatcaaaat ggcaatcgg ccgatacat ttcgcatgt cagaatgaa aagtatggt tgcgacatt gtaaaattt cttttagga aagggtcaa agtttggat gtttgcaat aaaaatggt agtttgcct atgcatttg gcgtgtcaa tatgctagt acaaatgtc gtaaaagtt cttcttgat aatagtatg aaggtaaat gtttgcaga caagataga caagcgttt ataaacctc gcacgtcta aatgaaaag catggctac gagattgtg aaattacta agtaattgt ttacaataa
- >TC012189 RB
atggcacac aatagctcg gaaaataac aacataaga caaaatcta cccgaaccc gaacccgaa cctccaacc acaactcct tcgacgact ttcaaaaaa cgaagtggt actacagat cttggcaaa gagtacgaa cacttggtc atttcttat ctaattctc aaattaatt aaaaataac caaattgaa aattttttt atttcttcc aacgatagc agttacggt gctttcgat gatgttgta ttggaagtg agatacaca ggagaaaaa caatacgaa acgtatgct ctacagtta aaacacaaa gaaaatggc acattaaaa attcaagtt ctaactcaa gcaaagggt aatttcagt attggaaaa tattttgaa gactatgag acaaactta tcaaaaaga tcggctaaa gtggttttg tttacgaat cttaaattt caaaacggt tcaggacaa ttgacattg aatttgaaa aatgaggaa aagaagtat catatcaca gaagttcaa cagggcaag cttaattta atgacaact tcagaggga aaggtgtac aagattgat tctgaagaa gcaattttt tttgaaaaa ttttatcta tatactgac cagcgaaat gttgatgat ttaaaaaag aaatgttta tcagaattt gacaacatt ttttcttct ggaaaaact ggcttcaac gagtactta acatttata accaattgg agtttaaaa gttggaaga aaaaaagaa aagttgagt aaaacctgg attcaagaa gttttgtcg atgctcgtg ttaactcct ctaattcag ccattagtg ttcgtctca gaagcgaca agtgaaaat gcaaagtta tttcaagac gctgtttca aagttccaa attacgatt ctcaacgaa agcagttac gaaaaagtc aaatctgtt tggaaaaac gcaactcaa aatattgat ttcaaggaa cttaacaaa attaacacg aaataccag gtaatggcg gacaacatt cttaataca gaagaatta gtaaagaaa aacggcacc gaagttaat acgattctt tggttgatg ggcaaatgt cccctaata gtaaaaaac gacaaaaag attaccaaa gttatagat ttgttaagt gatgaacaa aaattgatt attcttcac tctggacag aaggggaag acggatttg caacaccaa aactgtttt caaaatttg ggcaatatt aaatccgaa gagaaactt tatgatgat attttacaa aattttacc tttgcaata gaaggacaa acacctgct ttattaaaa gacttggtt cgcatttgc aaagaagtg gaacataca gtaacaacg gatgatctt ctgcaaatg atggaagga aactttgaa attggaaaa tcagaggaa ctctcactg ccaccttac tacgtgcca agagctatg tctcgagtc ttgataaat tcggaatat ttgaataca ttttcaaac gacacctta gttatcatt agttgtgtg acaaattta aataccttc aagaagaga tttgaagaa tacaagatt gttgacatt gatgagcat tttaaaaat gaagatgtg aagaaaaag aataaggaa aggaaaact atctacatc accaggaat aagtgcgtg caggaacaa tttgataca ttgtgttca agatatccg gaaaaaaga aactgccac cttatgaaa tatcacaat ttgtattct ttagaatgg gtcagaagt tctaatgga gtcaaggat ttgcaaaaa ttcaaaatg tcaaaagaa gatcaagac atcaaagaa gttcagtta cttgcaact ttttttaaa gataatttc aacattcag aaaacagca aatgttgat cacttcctg gaaattgac agaataagg gactttggc tcaaaaacc ttgtcagat ttccggcta aataaaacc aattccatt gaagaatta gaattcttt cgtaattca catgacaat ctcatcaac gtcgtatgt gaaaatcca ggaatgggt aaaacaacg ttaatgaaa agtttgaag aagaacact ttttcttgt ctttggacc atcttcata gtggcagga aaccacgct aaacacttt cgggacaat ccaaaagga gacgttgga aaattcttg gattatatc acgcaagcc aattataga aataatgaa acagagatg caaattttg cagaatttt gtgtctaat aaacaaata gttttggtt tgggatggt cttgatgaa gtcagcgat aaaactctg gagattata aagcgtatt gtacatggt gtttcccga aagaaaatt acacagtgg ataacttct agaattaac ttagaagat gtaatggaa agcgaattt ggtgttttt tgtagacgc attgaacag tttaagaaa gaagagtta caatctttt ataaaaaat cgcttaacc tgttcagat gacgaatta ttaaacatt tttgatgaa attaaattg aacattcaa cttgttcgt tataacagt attttggaa ataccatta catatatac attttaaca gaattactt attagtgat aaagaaacg actttgact ttgttagga aacaaggag caaatattt tcggtgagt gatatatat cagcgtttt gttgacaaa gtaattgaa caaaattgt aaagaaaaa tctaactac gattttgaa aaggaatcc attattaaa aaatttgat gaagaaaaa acaaaagca attaacgac tataagaaa atagccatc tattattat ataacagaa ttatccaac aaaactaaa tgtagaaaa attctaaaa caaataaaa gaaaaaaaa gatcctttc ggttttata ataaaagtc acagaggag atgtcgcca cagttttcg cataaatct ttcggggag tattttgca gctttatat ctttctgaa aatcttgaa aaaatttac aaaatttca gattttata cagaatgac aaatacgat aacattcga tttttccta gacttgatt ttggcaaaa aattcaaaa gctcatatt gcagtgttg tataaaaat agtatatta ttagatcac tgtagtaat gaagattta aatcgtaaa gatttactt gggcgtaat agttttgaa ctgtcatgt gagtgggat aagcgatat cctctttta aaaacgcaa aaacgcaaa gaatgttat tctatttcc attacattt ccagtaact attcctgca aaaaaaaga aaaaaaaac atttcaaat tttctaaaa gaatatgat gatgcagat tttaaatta aaaaaaaat attcttttg tttcctttt atagtaaca agtaataca aattattat accgaaatt gacaaaatt aatattcct tcaattttt tattatgca gtccaacat aatcacgca cttgtcttc gaatacttt ggacaagtt ccatttatt ctcataaaa acgtttatt cctgatatc atttggaca cttttagac ctagcaatc cactcaaag gcaattcat tcgataaat ttccttttg cgtaatcca cagtatcga aatcagtta aaggcgaat gtaagtctt tcatacagc ttgtgtacc ttacagtca cgcaaaatg ttaaaggct ttcactgac agtggatgg aatataaat gaaatacat agcgatggt atggctgtt gtacatcta gcttgtatt agcgcagac ttattaaca cttcaattt ataattgaa aatgacgga gatgtaaat gtttgcaat aaagatggt cgtttgccc atacatttt gcatgtcaa tatggcaac ataaatgtc gtaaaattt ctttttgat catggtgca aagatagat gtttgtgat caaaatggc aatcggcca ttagatttg gcatgtgtg aatgaaaag tatggttgc gacattgta aaatttctt ttagaaaac ggtcaaaat ttgaatatt tgcaataaa aatggtagc ttgcctatg catttggta tgtctattt ggtagcata agcgtcgta aaatttctt tttgatcat ggtgcaaag atagatgtt tgtgatcaa aatggcgat cggccatta catttcgca tgtgtgaat gaaaagtat ggttgcgac attgtaaaa tttctttta gaaaacggt caaagtttg aatatttgc aataaaatt ggtagcttg cctatgcat ttggcatgt ctatttggt agcataagc gtcgtaaaa tttcttttt gatcatggt gcaaaaata gatgtttgt gatcaaaag ggcaatcgg ccattacat tttgcatgt cagaatgaa aagtatggt tgcgacatt gtaaaattt cttttagaa aacggtcaa agtttgaat atttgcaat aaaaatggt agcgtgcct atgcatttg gtatgtcta tttggtagc ataagcgtc gtaaaattt ctttttgat catggtgca aagatagat gtttacgat caaaatggc aatcggccg atacatttc gcatgtctg aatgaaaag tatggttgc gacattgta aaatttctt ttaggaaag ggtcaaagt ttggatgtt tgcaataaa aatggtcat ttgcctata cattttgca tatcaaaat ggtagcata aatgtcgta aaatttctt tttgatcat ggtgcaaag atagatgtt tacgatcaa gagggcagt cggccatta catttcgca tgtgtgaat gaaaagtat ggttgcgac attgtaaaa tttctttta gaaaacggt caaagtttg aatatttgc aataaaaat ggtagcgtg cctatgcat ttggcatgt ctatttggt agcataagc gtcgtaaaa tttcttttt gatcgtggt gcaaagata gatgtttgt gatcaaaag ggcaatcgg ccattacat tttgcatgt cagaatgaa aagtatggt tgcgacatt gtaaaattt cttttagaa aacggtcaa aatttgaat atttgcaat aaaaatggt agcttgcct atgcatttg gcatgtcta tttggtagc ataagcgtc gtaaaattt ctttttgat catggtgca aagatagat gtttacgat caaaatggc aatcggccg atacatttc gcatgtcag aatgaaaag tatggttgc gacattgta aaatttctt ttaggaaag ggtcaaagt ttggatgtt tgcaataaa aatggtagt ttgcctatg catttggcg tgtcaatat gctagtaca aatgtcgta aaagttctt cttgataat agtatgaag gtaaatgtt tgcagacaa gatagacaa gcgtttata aacctcgca cgtctaaat gaaaagcat ggctacgag attgtgaaa ttactaagt aattgttta caataa
- >TC012189 RA
aattatttc gataccaat taacatcat gggtatcgt acacttata acaaatggc acacaatag ctcggaaaa taacaacat aagacaaaa tctacccga acccgaacc cgaacctcc aaccacaac tccttcgac ggtgagttt ttttgtgtt tgtgataat tgatcaacg atcagcgat acttggttt catcataat ataaaattt atagatatt cttaaattt taacaatga atttgattt tcaatttca aggcggtta aaagttgtg gtatcggtt ttcagactt tcaaaaaac gaagtggta ctacagatc ttggcaaag agtacgaac acttggtca tttcttatc taattctca aattaatta aaaataacc aaattgaaa attttttta tttcttcca acgatagca gttacggtg ctttcgatg atgttgtat tggaagtga gatacacag gagaaaaac aatacgaaa cgtatgctc tacagttaa aacacaaag aaaatggca cattaaaaa ttcaagttc taactcaag caaagggta atttcagta ttggaaaat attttgaag actatgaga caaacttat caaaaagat cggctaaag tggttttgt ttacgaatc ttaaatttc aaaacggtt caggacaat tgacattga atttgaaaa atgaggaaa agaagtatc atatcacag aagttcaac agggcaagc ttaatttaa tgacaactt cagagggaa aggtgtaca agattgatt ctgaagaag caatttttt ttgaaaaat tttatctat atactgacc agcgaaatg ttgatgatt taaaaaaga aatgtttat cagaatttg acaacattt tttcttctg gaaaaactg gcttcaacg agtacttaa catttataa ccaattgga gtttaaaag ttggaagaa aaaaagaaa agttgagta aaacctgga ttcaagaag ttttgtcga tgctcgtgt taactcctc taattcagc cattagtgt tcgtctcag aagcgacaa gtgaaaatg caaagttat ttcaagacg ctgtttcaa agttccaaa ttacgattc tcaacgaaa gcagttacg aaaaagtca aatctgttt ggaaaaacg caactcaaa atattgatt tcaaggaac ttaacaaaa ttaacacga aataccagg taatggcgg acaacattc ttaatacag aagaattag taaagaaaa acggcaccg aagttaata cgattcttt ggttgatgg gcaaatgtc ccctaatag taaaaaacg acaaaaaga ttaccaaag ttatagatt tgttaagtg atgaacaaa aattgatta ttcttcact ctggacaga aggggaaga cggatttgc aacaccaaa actgttttc aaaatttgg gcaatatta aatccgaag agaaacttt atgatgata ttttacaaa attttacct ttgcaatag aaggacaaa cacctgctt tattaaaag acttggttc gcatttgca aagaagtgg aacatacag taacaacgg atgatcttc tgcaaatga tggaaggaa actttgaaa ttggaaaat cagaggaac tctcactgc caccttact acgtgccaa gagctatgt ctcgagtct tgataaatt cggaatatt tgaatacat tttcaaacg acaccttag ttatcatta gttgtgtga caaatttaa ataccttca agaagagat ttgaagaat acaagattg ttgacattg atgagcatt ttaaaaatg aagatgtga agaaaaaga ataaggaaa ggaaaacta tctacatca ccaggaata agtgcgtgc aggaacaat ttgatacat tgtgttcaa gatatccgg aaaaaagaa actgccacc ttatgaaat atcacaatt tgtattctt tagaatggg tcagaagtt ctaatggag tcaaggatt tgcaaaaat tcaaaatgt caaaagaag atcaagaca tcaaagaag ttcagttac ttgcaactt tttttaaag ataatttca acattcaga aaacagcaa atgttgatc acttcctgg aaattgaca gaataaggg actttggct caaaaacct tgtcagatt tccggctaa ataaaacca attccattg aagaattag aattctttc gtaattcac atgacaatc tcatcaacg tcgtatgtg aaaatccag gaatgggta aaacaacgt taatgaaaa gtttgaaga agaacactt tttcttgtc tttggacca tcttcatag tggcaggaa accacgcta aacactttc gggacaatc caaaaggag acgttggaa aattcttgg attatatca cgcaagcca attatagaa ataatgaaa cagagatgc aaattttgc agaattttg tgtctaata aacaaatag ttttggttt gggatggtc ttgatgaag tcagcgata aaactctgg agattataa agcgtattg tacatggtg tttcccgaa agaaaatta cacagtgga taacttcta gaattaact tagaagatg taatggaaa gcgaatttg gtgtttttt gtagacgca ttgaacagt ttaagaaag aagagttac aatctttta taaaaaatc gcttaacct gttcagatg acgaattat taaacattt ttgatgaaa ttaaattga acattcaac ttgttcgtt ataacagta ttttggaaa taccattac atatataca ttttaacag aattactta ttagtgata aagaaacga ctttgactt tgttaggaa acaaggagc aaatatttt cggtgagtg atatatatc agcgttttg ttgacaaag taattgaac aaaattgta aagaaaaat ctaactacg attttgaaa aggaatcca ttattaaaa aatttgatg aagaaaaaa caaaagcaa ttaacgact ataagaaaa tagccatct attattata taacagaat tatccaaca aaactaaat gtagaaaaa ttctaaaac aaataaaag aaaaaaaag atcctttcg gttttataa taaaagtca cagaggaga tgtcgccac agttttcgc ataaatctt tcggggagt attttgcag ctttatatc tttctgaaa atcttgaaa aaatttaca aaatttcag attttatac agaatgaca aatacgata acattcgat ttttcctag acttgattt tggcaaaaa attcaaaag ctcatattg cagtgttgt ataaaaata gtatattat tagatcact gtagtaatg aagatttaa atcgtaaag atttacttg ggcgtaata gttttgaac tgtcatgtg agtgggata agcgatatc ctcttttaa aaacgcaaa aacgcaaag aatgttatt ctatttcca ttacatttc cagtaacta ttcctgcaa aaaaaagaa aaaaaaaca tttcaaatt ttctaaaag aatatgatg atgcagatt ttaaattaa aaaaaaata ttcttttgt ttcctttta tagtaacaa gtaatacaa attattata ccgaaattg acaaaatta atattcctt caatttttt attatgcag tccaacata atcacgcac ttgtcttcg aatactttg gacaagttc catttattc tcataaaaa cgtttattc ctgatatca tttggacac ttttagacc tagcaatcc actcaaagg caattcatt cgataaatt tccttttgc gtaatccac agtatcgaa atcagttaa aggcgaatg taagtcttt catacagct tgtgtacct tacagtcac gcaaaatgt taaaggctt tcactgaca gtggatgga atataaatg aaatacata gcgatggta tggctgttg tacatctag cttgtatta gcgcagact tattaacac ttcaattta taattgaaa atgacggag atgtaaatg tttgcaata aagatggtc gtttgccca tacattttg catgtcaat atggcaaca taaatgtcg taaaatttc tttttgatc atggtgcaa agatagatg tttgtgatc aaaatggca atcggccat tagatttgg catgtgtga atgaaaagt atggttgcg acattgtaa aatttcttt tagaaaacg gtcaaaatt tgaatattt gcaataaaa atggtagct tgcctatgc atttggtat gtctatttg gtagcataa gcgtcgtaa aatttcttt ttgatcatg gtgcaaaga tagatgttt gtgatcaaa atggcgatc ggccattac atttcgcat gtgtgaatg aaaagtatg gttgcgaca ttgtaaaat ttcttttag aaaacggtc aaagtttga atatttgca ataaaattg gtagcttgc ctatgcatt tggcatgtc tatttggta gcataagcg tcgtaaaat ttctttttg atcatggtg caaaaatag atgtttgtg atcaaaagg gcaatcggc cattacatt ttgcatgtc agaatgaaa agtatggtt gcgacattg taaaatttc ttttagaaa acggtcaaa gtttgaata tttgcaata aaaatggta gcgtgccta tgcatttgg tatgtctat ttggtagca taagcgtcg taaaatttc tttttgatc atggtgcaa agatagatg tttacgatc aaaatggca atcggccga tacatttcg catgtctga atgaaaagt atggttgcg acattgtaa aatttcttt taggaaagg gtcaaagtt tggatgttt gcaataaaa atggtcatt tgcctatac attttgcat atcaaaatg gtagcataa atgtcgtaa aatttcttt ttgatcatg gtgcaaaga tagatgttt acgatcaag agggcagtc ggccattac atttcgcat gtgtgaatg aaaagtatg gttgcgaca ttgtaaaat ttcttttag aaaacggtc aaagtttga atatttgca ataaaaatg gtagcgtgc ctatgcatt tggcatgtc tatttggta gcataagcg tcgtaaaat ttctttttg atcgtggtg caaagatag atgtttgtg atcaaaagg gcaatcggc cattacatt ttgcatgtc agaatgaaa agtatggtt gcgacattg taaaatttc ttttagaaa acggtcaaa atttgaata tttgcaata aaaatggta gcttgccta tgcatttgg catgtctat ttggtagca taagcgtcg taaaatttc tttttgatc atggtgcaa agatagatg tttacgatc aaaatggca atcggccga tacatttcg catgtcaga atgaaaagt atggttgcg acattgtaa aatttcttt taggaaagg gtcaaagtt tggatgttt gcaataaaa atggtagtt tgcctatgc atttggcgt gtcaatatg ctagtacaa atgtcgtaa aagttcttc ttgataata gtatgaagg taaatgttt gcagacaag atagacaag cgtttataa acctcgcac gtctaaatg aaaagcatg gctacgaga ttgtgaaat tactaagta attgtttac aataaagtg tcaattttc taacattac atcgagcat gttagaatg aaaagttta gagccagtt tacagaaca ttttaattg caattaaat tttaatcgc gattaactt gacatttgt caatgcatt ttaaatgac aacttaatt cgaattagt ttaataaat cttaatatc gctttctgt aaaccggcc cttagtggg tcattgtat agaattact tttagaagt ttgaatgtt cgtagtctc tgtttcaaa actacacgg aagtgccaa cgtcgcatt ttaccgaat tatttttta aaataaagg tgataaaaa agtgctgaa acaataaca attgtcgtc gaagagtaa acattagta cttaatata ctattataa tgttccaaa gttagttta ttaaaatat ttggatggt gacgttacg gataatgtg taaacttgg cgagtttgt aaactagcc acaccgttg gctattttg ataaaaaaa atgcagttt caactaaaa tagcggcta ttttacata ctactcaag aatcttagc taatttact tgaaaaata gagtagaaa tttatattc gcattttaa ccattgtaa gtctattat atgtatgtt ggattgttg gttggttta taaattagc taaaacaag taaacaacc aatgtttta aattgttta taaacctca tttggtaat caatcaagt tagtcagca agttgatta gttcgtcaa ttacaatag ctaaagcat tagatgttt tgcgtgaag agaaagtat ggttttccg ttttcatat tagctatag aatgcctcg gatagttca taaactatt gaactattt tatctttca agtaaatta ctaaattac ccaggattc t
- >TC012189 RB
aagtagggt gacataaaa aaattcttg aaaaatgac cacattttt aaagtagat actcgatga tgtatacaa ggtgttggc actttatgt atgctgaaa gtgataaat tatttcgat accaattaa catcatggg tatcgtaca cttataaca aatggcaca caatagctc ggaaaataa caacataag acaaaatct acccgaacc cgaacccga acctccaac cacaactcc ttcgacgac tttcaaaaa acgaagtgg tactacaga tcttggcaa agagtacga acacttggt catttctta tctaattct caaattaat taaaaataa ccaaattga aaatttttt tatttcttc caacgatag cagttacgg tgctttcga tgatgttgt attggaagt gagatacac aggagaaaa acaatacga aacgtatgc tctacagtt aaaacacaa agaaaatgg cacattaaa aattcaagt tctaactca agcaaaggg taatttcag tattggaaa atattttga agactatga gacaaactt atcaaaaag atcggctaa agtggtttt gtttacgaa tcttaaatt tcaaaacgg ttcaggaca attgacatt gaatttgaa aaatgagga aaagaagta tcatatcac agaagttca acagggcaa gcttaattt aatgacaac ttcagaggg aaaggtgta caagattga ttctgaaga agcaatttt ttttgaaaa attttatct atatactga ccagcgaaa tgttgatga tttaaaaaa gaaatgttt atcagaatt tgacaacat tttttcttc tggaaaaac tggcttcaa cgagtactt aacatttat aaccaattg gagtttaaa agttggaag aaaaaaaga aaagttgag taaaacctg gattcaaga agttttgtc gatgctcgt gttaactcc tctaattca gccattagt gttcgtctc agaagcgac aagtgaaaa tgcaaagtt atttcaaga cgctgtttc aaagttcca aattacgat tctcaacga aagcagtta cgaaaaagt caaatctgt ttggaaaaa cgcaactca aaatattga tttcaagga acttaacaa aattaacac gaaatacca ggtaatggc ggacaacat tcttaatac agaagaatt agtaaagaa aaacggcac cgaagttaa tacgattct ttggttgat gggcaaatg tcccctaat agtaaaaaa cgacaaaaa gattaccaa agttataga tttgttaag tgatgaaca aaaattgat tattcttca ctctggaca gaaggggaa gacggattt gcaacacca aaactgttt tcaaaattt gggcaatat taaatccga agagaaact ttatgatga tattttaca aaattttac ctttgcaat agaaggaca aacacctgc tttattaaa agacttggt tcgcatttg caaagaagt ggaacatac agtaacaac ggatgatct tctgcaaat gatggaagg aaactttga aattggaaa atcagagga actctcact gccacctta ctacgtgcc aagagctat gtctcgagt cttgataaa ttcggaata tttgaatac attttcaaa cgacacctt agttatcat tagttgtgt gacaaattt aaatacctt caagaagag atttgaaga atacaagat tgttgacat tgatgagca ttttaaaaa tgaagatgt gaagaaaaa gaataagga aaggaaaac tatctacat caccaggaa taagtgcgt gcaggaaca atttgatac attgtgttc aagatatcc ggaaaaaag aaactgcca ccttatgaa atatcacaa tttgtattc tttagaatg ggtcagaag ttctaatgg agtcaagga tttgcaaaa attcaaaat gtcaaaaga agatcaaga catcaaaga agttcagtt acttgcaac tttttttaa agataattt caacattca gaaaacagc aaatgttga tcacttcct ggaaattga cagaataag ggactttgg ctcaaaaac cttgtcaga tttccggct aaataaaac caattccat tgaagaatt agaattctt tcgtaattc acatgacaa tctcatcaa cgtcgtatg tgaaaatcc aggaatggg taaaacaac gttaatgaa aagtttgaa gaagaacac tttttcttg tctttggac catcttcat agtggcagg aaaccacgc taaacactt tcgggacaa tccaaaagg agacgttgg aaaattctt ggattatat cacgcaagc caattatag aaataatga aacagagat gcaaatttt gcagaattt tgtgtctaa taaacaaat agttttggt ttgggatgg tcttgatga agtcagcga taaaactct ggagattat aaagcgtat tgtacatgg tgtttcccg aaagaaaat tacacagtg gataacttc tagaattaa cttagaaga tgtaatgga aagcgaatt tggtgtttt ttgtagacg cattgaaca gtttaagaa agaagagtt acaatcttt tataaaaaa tcgcttaac ctgttcaga tgacgaatt attaaacat ttttgatga aattaaatt gaacattca acttgttcg ttataacag tattttgga aataccatt acatatata cattttaac agaattact tattagtga taaagaaac gactttgac tttgttagg aaacaagga gcaaatatt ttcggtgag tgatatata tcagcgttt tgttgacaa agtaattga acaaaattg taaagaaaa atctaacta cgattttga aaaggaatc cattattaa aaaatttga tgaagaaaa aacaaaagc aattaacga ctataagaa aatagccat ctattatta tataacaga attatccaa caaaactaa atgtagaaa aattctaaa acaaataaa agaaaaaaa agatccttt cggttttat aataaaagt cacagagga gatgtcgcc acagttttc gcataaatc tttcgggga gtattttgc agctttata tctttctga aaatcttga aaaaattta caaaatttc agattttat acagaatga caaatacga taacattcg atttttcct agacttgat tttggcaaa aaattcaaa agctcatat tgcagtgtt gtataaaaa tagtatatt attagatca ctgtagtaa tgaagattt aaatcgtaa agatttact tgggcgtaa tagttttga actgtcatg tgagtggga taagcgata tcctctttt aaaaacgca aaaacgcaa agaatgtta ttctatttc cattacatt tccagtaac tattcctgc aaaaaaaag aaaaaaaaa catttcaaa ttttctaaa agaatatga tgatgcaga ttttaaatt aaaaaaaaa tattctttt gtttccttt tatagtaac aagtaatac aaattatta taccgaaat tgacaaaat taatattcc ttcaatttt ttattatgc agtccaaca taatcacgc acttgtctt cgaatactt tggacaagt tccatttat tctcataaa aacgtttat tcctgatat catttggac acttttaga cctagcaat ccactcaaa ggcaattca ttcgataaa tttcctttt gcgtaatcc acagtatcg aaatcagtt aaaggcgaa tgtaagtct ttcatacag cttgtgtac cttacagtc acgcaaaat gttaaaggc tttcactga cagtggatg gaatataaa tgaaataca tagcgatgg tatggctgt tgtacatct agcttgtat tagcgcaga cttattaac acttcaatt tataattga aaatgacgg agatgtaaa tgtttgcaa taaagatgg tcgtttgcc catacattt tgcatgtca atatggcaa cataaatgt cgtaaaatt tctttttga tcatggtgc aaagataga tgtttgtga tcaaaatgg caatcggcc attagattt ggcatgtgt gaatgaaaa gtatggttg cgacattgt aaaatttct tttagaaaa cggtcaaaa tttgaatat ttgcaataa aaatggtag cttgcctat gcatttggt atgtctatt tggtagcat aagcgtcgt aaaatttct ttttgatca tggtgcaaa gatagatgt ttgtgatca aaatggcga tcggccatt acatttcgc atgtgtgaa tgaaaagta tggttgcga cattgtaaa atttctttt agaaaacgg tcaaagttt gaatatttg caataaaat tggtagctt gcctatgca tttggcatg tctatttgg tagcataag cgtcgtaaa atttctttt tgatcatgg tgcaaaaat agatgtttg tgatcaaaa gggcaatcg gccattaca ttttgcatg tcagaatga aaagtatgg ttgcgacat tgtaaaatt tcttttaga aaacggtca aagtttgaa tatttgcaa taaaaatgg tagcgtgcc tatgcattt ggtatgtct atttggtag cataagcgt cgtaaaatt tctttttga tcatggtgc aaagataga tgtttacga tcaaaatgg caatcggcc gatacattt cgcatgtct gaatgaaaa gtatggttg cgacattgt aaaatttct tttaggaaa gggtcaaag tttggatgt ttgcaataa aaatggtca tttgcctat acattttgc atatcaaaa tggtagcat aaatgtcgt aaaatttct ttttgatca tggtgcaaa gatagatgt ttacgatca agagggcag tcggccatt acatttcgc atgtgtgaa tgaaaagta tggttgcga cattgtaaa atttctttt agaaaacgg tcaaagttt gaatatttg caataaaaa tggtagcgt gcctatgca tttggcatg tctatttgg tagcataag cgtcgtaaa atttctttt tgatcgtgg tgcaaagat agatgtttg tgatcaaaa gggcaatcg gccattaca ttttgcatg tcagaatga aaagtatgg ttgcgacat tgtaaaatt tcttttaga aaacggtca aaatttgaa tatttgcaa taaaaatgg tagcttgcc tatgcattt ggcatgtct atttggtag cataagcgt cgtaaaatt tctttttga tcatggtgc aaagataga tgtttacga tcaaaatgg caatcggcc gatacattt cgcatgtca gaatgaaaa gtatggttg cgacattgt aaaatttct tttaggaaa gggtcaaag tttggatgt ttgcaataa aaatggtag tttgcctat gcatttggc gtgtcaata tgctagtac aaatgtcgt aaaagttct tcttgataa tagtatgaa ggtaaatgt ttgcagaca agatagaca agcgtttat aaacctcgc acgtctaaa tgaaaagca tggctacga gattgtgaa attactaag taattgttt acaataaag tgtcaattt tctaacatt acatcgagc atgttagaa tgaaaagtt tagagccag tttacagaa cattttaat tgcaattaa attttaatc gcgattaac ttgacattt gtcaatgca ttttaaatg acaacttaa ttcgaatta gtttaataa atcttaata tcgctttct gtaaaccgg cccttagtg ggtcattgt atagaatta cttttagaa gtttgaatg ttcgtagtc tctgtttca aaactacac ggaagtgcc aacgtcgca ttttaccga attattttt taaaataaa ggtgataaa aaagtgctg aaacaataa caattgtcg tcgaagagt aaacattag tacttaata tactattat aatgttcca aagttagtt tattaaaat atttggatg gtgacgtta cggataatg tgtaaactt ggcgagttt gtaaactag ccacaccgt tggctattt tgataaaaa aaatgcagt ttcaactaa aatagcggc tattttaca tactactca agaatctta gctaattta cttgaaaaa tagagtaga aatttatat tcgcatttt aaccattgt aagtctatt atatgtatg ttggattgt tggttggtt tataaatta gctaaaaca agtaaacaa ccaatgttt taaattgtt tataaacct catttggta atcaatcaa gttagtcag caagttgat tagttcgtc aattacaat agctaaagc attagatgt tttgcgtga agagaaagt atggttttc cgttttcat attagctat agaatgcct cggatagtt cataaacta ttgaactat tttatcttt caagtaaat tactaaatt acccaggat tct
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