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Gene information
General information
TC012175 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3903892..3905640
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012175
    iBeetle screen iB_05187

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012175 RA
      MSGMLYGGD EIGALVFDP GHHSLRVGY AQEDTPKAE 
      IPAVVGVAP DDTPKIEPE TKPDESNVS STAPAWKYY 
      IDTTFLHTP RPSMDLHSY MKDGMIENW DLFEKIVDY 
      SYDKIIQSE SQYHPVLFS ECPWNLRHK REKLTEIMF 
      EKYKVPAFF LVKNAALAA FANGRATAL VIDSGATHT 
      SAVPVLDGY VITNAVVKS PLGGDYLIL KAREMLEHM 
      GIDLTPAAL IASKEVVRD KDKPKYMKK KLSFQPTNS 
      WMAYMIKKT VQDFQQNVI QVSESPFDE RIAAGFPTI 
      QHEFPTGYR QDFGPERFK LAEVLFDHA MLGAGHIAA 
      TSAGMCDVD IRPALYSSV VLTGGNSLV QGFSDRLSR 
      DLSSRTPGS LRLKMIAAN GTVERRFGS WVGGSILAS 
      IGTFQQMWM SMQEYQEAG KAQIDRKCP
    CDS sequence
    • >TC012175 RA
      atgtctgga atgttatac ggcggcgac gaaatcggg 
      gctctagtc ttcgaccct gggcaccac tccttgcgc 
      gtaggctac gctcaggag gacacccct aaagccgaa 
      atcccggcc gtggttggc gttgcgccc gacgacacc 
      ccgaaaatc gagccggaa accaaacca gacgaaagc 
      aacgtgtcc tcgacagcg ccagcttgg aagtactac 
      attgacacg accttttta cacacgccg aggcccagc 
      atggacctg cacagctac atgaaggac gggatgatt 
      gaaaactgg gacttgttc gagaaaatt gtcgactat 
      tcgtacgat aaaattatc cagtcagaa tctcagtac 
      caccccgtc ttgttctcc gagtgccct tggaacctg 
      cgccacaag cgggagaag ctcaccgaa atcatgttc 
      gaaaagtac aaagtcccg gcgtttttc ctcgtgaag 
      aacgcggct ttggcggcg tttgccaac ggccgcgcc 
      acggccctc gtcatagac agcggggcc acgcacact 
      tccgccgta cctgtttta gatggctat gtgatcaca 
      aacgccgtt gttaaatca ccacttggt ggcgactac 
      ctcatttta aaagcgcga gaaatgcta gagcacatg 
      gggattgac ctcacgccg gctgcgctc atagcgagc 
      aaagaagtc gtgcgcgat aaggacaaa ccaaagtac 
      atgaaaaag aagctctct ttccagcca accaacagc 
      tggatggcc tacatgatc aaaaaaacg gttcaggat 
      ttccagcag aacgtcatt caagtgtct gagtcgccc 
      tttgacgag agaattgct gcgggtttt cccacgatt 
      cagcacgag ttccccaca gggtatcgc caggacttc 
      ggtccagag cgctttaaa ctggctgag gtcctgttc 
      gatcatgcg atgttgggg gcagggcat atagctgca 
      actagcgcc ggaatgtgc gacgttgac ataagaccg 
      gctctctac agctctgtg gttctgacc ggtggtaat 
      tcattagta caaggattt agtgatagg ttaagccgg 
      gatttatcg tcccggact cccggttct ttacggtta 
      aaaatgata gcggccaat ggcaccgtc gagcgacgg 
      tttggctcg tgggtcggt gggtcgatc cttgcgtcc 
      attggtact ttccaacag atgtggatg tccatgcag 
      gagtatcaa gaggccggc aaggcccag attgacaga 
      aagtgtcct taa
    mRNA sequence
    • >TC012175 RA
      ccaaagggg tgcatttta atgcgaatt cgtcgaaaa 
      tgaaattaa atcaaattt attccgcct gccgtgtag 
      ataacccac catacccca ccctagtaa ctttccttc 
      cacgttgtt ggtgcgaat cggacgctt ctcaagccc 
      ggcaaatgt caaacatac agataacct aatttatcg 
      tgtattttt tggtttttg ttcattaat aagtatttt 
      tctttgaaa atgtctgga atgttatac ggcggcgac 
      gaaatcggg gctctagtc ttcgaccct gggcaccac 
      tccttgcgc gtaggctac gctcaggag gacacccct 
      aaagccgaa atcccggcc gtggttggc gttgcgccc 
      gacgacacc ccgaaaatc gagccggaa accaaacca 
      gacgaaagc aacgtgtcc tcgacagcg ccagcttgg 
      aagtactac attgacacg accttttta cacacgccg 
      aggcccagc atggacctg cacagctac atgaaggac 
      gggatgatt gaaaactgg gacttgttc gagaaaatt 
      gtcgactat tcgtacgat aaaattatc cagtcagaa 
      tctcagtac caccccgtc ttgttctcc gagtgccct 
      tggaacctg cgccacaag cgggagaag ctcaccgaa 
      atcatgttc gaaaagtac aaagtcccg gcgtttttc 
      ctcgtgaag aacgcggct ttggcggcg tttgccaac 
      ggccgcgcc acggccctc gtcatagac agcggggcc 
      acgcacact tccgccgta cctgtttta gatggctat 
      gtgatcaca aacgccgtt gttaaatca ccacttggt 
      ggcgactac ctcatttta aaagcgcga gaaatgcta 
      gagcacatg gggattgac ctcacgccg gctgcgctc 
      atagcgagc aaagaagtc gtgcgcgat aaggacaaa 
      ccaaagtac atgaaaaag aagctctct ttccagcca 
      accaacagc tggatggcc tacatgatc aaaaaaacg 
      gttcaggat ttccagcag aacgtcatt caagtgtct 
      gagtcgccc tttgacgag agaattgct gcgggtttt 
      cccacgatt cagcacgag ttccccaca gggtatcgc 
      caggacttc ggtccagag cgctttaaa ctggctgag 
      gtcctgttc gatcatgcg atgttgggg gcagggcat 
      atagctgca actagcgcc ggaatgtgc gacgttgac 
      ataagaccg gctctctac agctctgtg gttctgacc 
      ggtggtaat tcattagta caaggattt agtgatagg 
      ttaagccgg gatttatcg tcccggact cccggttct 
      ttacggtta aaaatgata gcggccaat ggcaccgtc 
      gagcgacgg tttggctcg tgggtcggt gggtcgatc 
      cttgcgtcc attggtact ttccaacag atgtggatg 
      tccatgcag gagtatcaa gaggccggc aaggcccag 
      attgacaga aagtgtcct taaaatcta tttttaagc 
      caaacattt ccaatttta tcgtttaca tacatgtct 
      cagtatttt ttagacttt tcaccaagt aaggaattt 
      gtaataaaa tccacaaag taac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY