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Gene information
General information
TC012170 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 3957188..3958320
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC012170
      iBeetle screen iB_05185

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
      larva median decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
      Days post injection


      eclosion delayed (still pupa) - Number of animals showing the phenotype: 4 (16 dpi)
      Days post injection


      eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (19 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection


      Fertility
      egg production: number <50% of expected -
      ovary without detectable defects - Number of animals showing the phenotype: 4
      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC012170 RA
        MVKTKREYI FATCFLSGA AAIISFVSL GTQEWVTSP 
        AILSSAANA GGLRSENEN DYLKFGLFS GTFNQTTAQ 
        PATYEMTTV CSFGLNVCA LLCGDNMEE SLEKLYNGE 
        SDIKSLECP KIVTTTDKL YSEITDTFK LFVSKSSGE 
        DRTFINAGV WLCTIIFLL ISGMAGIVS AVLALWNTV 
        SNPYQNYLS IFGLYIYNA VAFFSVSLA IVLWGAMFS 
        QSLQANNLP TAKTFNDGF TTEGLSSLG YSYWINLAS 
        LACYFISIS VLCIRNWLI SIEPEVNVH IEDNADPVI 
        LLY
      CDS sequence
      • >TC012170 RA
        atggtaaaa acaaaacgg gagtacatt ttcgcgacg 
        tgtttcctg tcgggcgcc gccgccatt atctcattc 
        gtgtctttg ggaacccaa gaatgggta acatcgcca 
        gcgattttg tcctctgca gcaaatgca ggtggtttg 
        cgctcagaa aacgaaaac gactatctc aaattcgga 
        ctgtttagt gggactttc aaccaaacc accgctcaa 
        ccagcaact tacgaaatg acaactgtg tgctccttt 
        ggtttgaac gtttgtgct ttactgtgc ggcgataac 
        atggaagag agtttggag aagttgtac aatggggaa 
        agcgacata aagtcttta gagtgccct aaaattgta 
        acaaccact gataagctt tacagtgag ataaccgac 
        actttcaaa ctgtttgtg tccaaatcg agcggtgaa 
        gataggacg tttatcaat gcgggtgtc tggctgtgt 
        acgatcata ttcctgtta atatcggga atggcggga 
        attgtttcg gccgttttg gctctatgg aacacggtc 
        tcgaacccg tatcagaat tatttgagc atttttgga 
        ctgtacatt tacaacgct gtggcgttt ttcagcgtt 
        tcactagcg atcgtgctc tggggtgca atgttttcg 
        cagagtctg caagctaat aatttgcca acagctaaa 
        acgttcaat gatgggttc acaacagaa gggctctcc 
        agtttggga tattcctat tggataaac ctagcttct 
        ttggcctgt tattttatt agtataagc gtgctgtgt 
        atccggaac tggctcatt tccatagag ccggaagta 
        aatgtgcac atagaagat aatgcagat ccagtcatt 
        ctgttatat taa
      mRNA sequence
      • >TC012170 RA
        agtaaatca tggtaaaaa caaaacggg agtacattt 
        tcgcgacgt gtttcctgt cgggcgccg ccgccatta 
        tctcattcg tgtctttgg gaacccaag aatgggtaa 
        catcgccag cgattttgt cctctgcag caaatgcag 
        gtggtttgc gctcagaaa acgaaaacg actatctca 
        aattcggac tgtttagtg ggactttca accaaacca 
        ccgctcaac cagcaactt acgaaatga caactgtgt 
        gctcctttg gtttgaacg tttgtgctt tactgtgcg 
        gcgataaca tggaagaga gtttggaga agttgtaca 
        atggggaaa gcgacataa agtctttag agtgcccta 
        aaattgtaa caaccactg ataagcttt acagtgaga 
        taaccgaca ctttcaaac tgtttgtgt ccaaatcga 
        gcggtgaag ataggacgt ttatcaatg cgggtgtct 
        ggctgtgta cgatcatat tcctgttaa tatcgggaa 
        tggcgggaa ttgtttcgg ccgttttgg ctctatgga 
        acacggtct cgaacccgt atcagaatt atttgagca 
        tttttggac tgtacattt acaacgctg tggcgtttt 
        tcagcgttt cactagcga tcgtgctct ggggtgcaa 
        tgttttcgc agagtctgc aagctaata atttgccaa 
        cagctaaaa cgttcaatg atgggttca caacagaag 
        ggctctcca gtttgggat attcctatt ggataaacc 
        tagcttctt tggcctgtt attttatta gtataagcg 
        tgctgtgta tccggaact ggctcattt ccatagagc 
        cggaagtaa atgtgcaca tagaagata atgcagatc 
        cagtcattc tgttatatt aatttacag cattacatt 
        taacagaat atcactaat tatttattt gtaaaaatg 
        tacaattgt gttgttgta tacaagtgc cattttttg 
        taaatataa aattcaaat tata
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY