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Gene information
General information
TC012137 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4857159..4860827
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012137
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05181

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    vitellarium shortened - Number of animals showing the phenotype: 2

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    low validity due to technical reasons - should be repeated: adult phenotype not scorable,
    One animal shows a antenna and leg phenotype. Should be repeated! Ask Chris!
    Pupal morphology
    low validity due to technical reasons - should be repeated: adult phenotype not scorable,
    One animal shows a antenna and leg phenotype. Should be repeated! Ask Chris!
    Adult morphology
    low validity due to technical reasons - should be repeated: adult phenotype not scorable,
    One animal shows a antenna and leg phenotype. Should be repeated! Ask Chris!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012137 RA
      MVVESGTPP RRDLLRRSV VSLSTPHRK NTPTSDTPM 
      EPHDEEQER SGRRSMVVM SKKLSNISS PAADIGRSL 
      TEPEVQDHL RICKKLYSE NRISSRNAW ELHVIDAIK 
      KIAIQNQTN LMLVAGSSL DVGSKIYSL RIDDVHTRG 
      IQLASNVGK SDRKQAQAA EDAEELNGT ENPREKKRQ 
      RRKKIQTIL ADKTTLIDQ EGKSLFAPV KPLEPVVFA 
      TKESIDVSA IDNLLTSKV PVNPASHRF MLLSNEKFW 
      TTKPDEILR LENKIVEND LPEVSSFEL CVRFKNFEI 
      GTWTLEDEA NLLEDARDT LPPPVLDEN GVPIPELDG 
      SIHDVFEDN DIPGDDSVS DTEPQEVFQ MQFHGGINP 
      IVDAIPPEY RIETDYSYN TLVRSHSGT YIDKIWAGP 
      YHWKVKFMK KSTDRYSGA DQEVAKRAK KKKEPQPIN 
      FFELPEDAT KTFKKLVIK RMPCSTSCE KITYPVLDS 
      WVNKSLNYL YLKPNTPLP QLNIVEKNH DYEVAVYDY 
      ENPNDSLYC SQHNMDDAN DEPDAHTLE EDMPPIAHQ 
      QFLAGNLVD APEIVSVDH VTYAMHAKK IDMKKLKTC 
      MWKHLSGGE EGTVQPTSF SKVYEQIPR LAPEMSKNL 
      SPHLGFLSL LHLCNDHSL YLEAISDRG DFIIRDAKK
    CDS sequence
    • >TC012137 RA
      atggtggtc gaaagcgga acccctcct cgtagggac 
      cttttaagg cggtctgtg gtaagcctt tcgacaccg 
      catcggaaa aatacaccc acgagtgat actcctatg 
      gaaccccac gatgaagag caagagcgg tccgggaga 
      cgcagcatg gtggtgatg tcaaaaaag cttagtaac 
      atctcatca ccagccgcg gacataggg cgttccttg 
      acggagccc gaagttcaa gaccacttg cgtatatgc 
      aagaaattg tactcggaa aatagaatc tcctcaaga 
      aacgcctgg gagctgcat gtcatagac gcaataaaa 
      aaaatcgcc attcaaaac cagacaaat cttatgcta 
      gttgcggga tcttccctt gatgttggc tccaaaatt 
      tacagttta cgcatcgac gacgtgcac accagagga 
      atacagctt gccagcaat gttgggaaa agcgaccgg 
      aagcaagcc caagcggct gaagacgca gaagagctc 
      aatggcacc gaaaaccca cgcgagaaa aagcgacaa 
      aggcgcaaa aaaatccaa acgattctt gccgataaa 
      acaactctt attgatcaa gagggcaag tcgttgttt 
      gcccctgtc aagccactg gaaccggtc gtctttgcc 
      actaaagag agtatagac gttagcgca atcgataat 
      ctactcact tccaaagtt ccggtaaac ccggcaagt 
      cacagattc atgttgttg agtaacgaa aagttctgg 
      accacaaaa cccgacgag attttaaga cttgaaaat 
      aaaattgta gaaaatgac ttgcctgaa gtgtcgtcg 
      ttcgaacta tgcgtgcgt tttaaaaat tttgaaatt 
      gggacgtgg actcttgag gatgaagct aacttactg 
      gaagacgcg cgcgacact ttaccgccg cccgtgctt 
      gacgagaac ggggttcct attccggag ctcgatggt 
      tcaattcat gacgttttc gaagacaat gacattccc 
      ggagacgac tcagtcagc gatactgaa ccacaggag 
      gtgtttcag atgcagttt catggtgga atcaacccc 
      atagttgac gcaatacct ccagaatat cgcattgaa 
      accgattac agttacaac acgcttgta agaagtcac 
      tccggaaca tatatcgat aagatttgg gcgggaccg 
      taccattgg aaagttaaa tttatgaaa aaatcaaca 
      gaccgatat tcgggcgct gatcaggaa gttgctaaa 
      cgagcaaag aagaaaaag gaaccgcaa cctatcaat 
      tttttcgaa ctaccggaa gatgcaact aaaacattt 
      aaaaaatta gtgataaag agaatgcct tgctcgact 
      tcttgcgaa aaaattaca tatcctgtg cttgattcg 
      tgggtcaac aagtccctg aactacctt tatctgaaa 
      cctaatacg ccgttacca caactaaac atcgtagag 
      aagaaccac gattatgaa gtggctgtc tatgattat 
      gaaaatcca aatgattcg ctctactgc tcgcaacat 
      aacatggac gatgcaaac gatgaacca gacgctcat 
      acgctagag gaagatatg ccaccaatt gcacaccag 
      caatttttg gcagggaat ctggtcgat gcccctgaa 
      attgtttca gttgatcat gttacatac gcaatgcat 
      gccaagaaa atcgacatg aaaaagctc aaaacttgt 
      atgtggaag cacttgagt gggggggaa gaaggaact 
      gttcaacca accagcttc tcgaaagtg tatgaacag 
      ataccacgc ttagctcca gagatgagc aagaatcta 
      agtccgcat ttgggtttt ctgtctctg ttgcatctt 
      tgcaatgac catagtctg tatttggaa gcaatcagc 
      gatcgcgga gactttatt ataagggac gccaagaaa 
      tag
    mRNA sequence
    • >TC012137 RA
      tattcgtca aagacctga cccttttct tgaattcca 
      gctaaatcc atgaataca tgaacatta cagtcgacc 
      aggaattac tttcttaat tcgaccctg actactgac 
      aaaaataat taacggaat ttttgtatt gaaaattac 
      attacaggc agtaaatag tggttgcaa acgaaaatt 
      tctaaatta tttcataat cttataagc ttttgtcgc 
      atgctacca tcaaaacac ttgtgtcaa catgtcccc 
      taactgcaa tttctagta attttcggt ttagatgca 
      actgttggc aagagccgc ccaccaaat gacagctta 
      tttgaatga atggtgaag ttgctaacg gtttctcaa 
      tcttttgtg tttaaatat ctgtaaaaa attaacttt 
      ttctgcatt ttttcctga aaaatcagt agcaaaagc 
      atctagtgt tgctattac ctagtttat tctataatt 
      ctgacaact gctactgtg aaatttaga taatggtgg 
      tcgaaagcg gaacccctc ctcgtaggg accttttaa 
      ggcggtctg tggtaagcc tttcgacac cgcatcgga 
      aaaatacac ccacgagtg atactccta tggaacccc 
      acgatgaag agcaagagc ggtccggga gacgcagca 
      tggtggtga tgtcaaaaa agcttagta acatctcat 
      caccagccg cggacatag ggcgttcct tgacggagc 
      ccgaagttc aagaccact tgcgtatat gcaagaaat 
      tgtactcgg aaaatagaa tctcctcaa gaaacgcct 
      gggagctgc atgtcatag acgcaataa aaaaaatcg 
      ccattcaaa accagacaa atcttatgc tagttgcgg 
      gatcttccc ttgatgttg gctccaaaa tttacagtt 
      tacgcatcg acgacgtgc acaccagag gaatacagc 
      ttgccagca atgttggga aaagcgacc ggaagcaag 
      cccaagcgg ctgaagacg cagaagagc tcaatggca 
      ccgaaaacc cacgcgaga aaaagcgac aaaggcgca 
      aaaaaatcc aaacgattc ttgccgata aaacaactc 
      ttattgatc aagagggca agtcgttgt ttgcccctg 
      tcaagccac tggaaccgg tcgtctttg ccactaaag 
      agagtatag acgttagcg caatcgata atctactca 
      cttccaaag ttccggtaa acccggcaa gtcacagat 
      tcatgttgt tgagtaacg aaaagttct ggaccacaa 
      aacccgacg agattttaa gacttgaaa ataaaattg 
      tagaaaatg acttgcctg aagtgtcgt cgttcgaac 
      tatgcgtgc gttttaaaa attttgaaa ttgggacgt 
      ggactcttg aggatgaag ctaacttac tggaagacg 
      cgcgcgaca ctttaccgc cgcccgtgc ttgacgaga 
      acggggttc ctattccgg agctcgatg gttcaattc 
      atgacgttt tcgaagaca atgacattc ccggagacg 
      actcagtca gcgatactg aaccacagg aggtgtttc 
      agatgcagt ttcatggtg gaatcaacc ccatagttg 
      acgcaatac ctccagaat atcgcattg aaaccgatt 
      acagttaca acacgcttg taagaagtc actccggaa 
      catatatcg ataagattt gggcgggac cgtaccatt 
      ggaaagtta aatttatga aaaaatcaa cagaccgat 
      attcgggcg ctgatcagg aagttgcta aacgagcaa 
      agaagaaaa aggaaccgc aacctatca attttttcg 
      aactaccgg aagatgcaa ctaaaacat ttaaaaaat 
      tagtgataa agagaatgc cttgctcga cttcttgcg 
      aaaaaatta catatcctg tgcttgatt cgtgggtca 
      acaagtccc tgaactacc tttatctga aacctaata 
      cgccgttac cacaactaa acatcgtag agaagaacc 
      acgattatg aagtggctg tctatgatt atgaaaatc 
      caaatgatt cgctctact gctcgcaac ataacatgg 
      acgatgcaa acgatgaac cagacgctc atacgctag 
      aggaagata tgccaccaa ttgcacacc agcaatttt 
      tggcaggga atctggtcg atgcccctg aaattgttt 
      cagttgatc atgttacat acgcaatgc atgccaaga 
      aaatcgaca tgaaaaagc tcaaaactt gtatgtgga 
      agcacttga gtggggggg aagaaggaa ctgttcaac 
      caaccagct tctcgaaag tgtatgaac agataccac 
      gcttagctc cagagatga gcaagaatc taagtccgc 
      atttgggtt ttctgtctc tgttgcatc tttgcaatg 
      accatagtc tgtatttgg aagcaatca gcgatcgcg 
      gagacttta ttataaggg acgccaaga aatagtttt 
      tacttactt tgtagtctt aatttctac tgtgtaatt 
      ttttttaaa aagttaaaa atgtttaca gcttaaaaa 
      tttgtttcc actacacgt gaagaattc agtttaata 
      aaattatta caaatct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY