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Gene information
General information
TC012133 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5028039..5033206
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012133
    iBeetle screen iB_05180

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012133 RA
      MSYQNSISS KQAAREHVL AVSRDFVSQ PRLTYKTVT 
      GVNGPLVIL DDVKFPKFN EIVQLKLSD GSIRSGQVL 
      EVSGSKAVV QVFEGTSGI DAKHTVCEF TGDILRTPV 
      SEDMLGRVF NGSGKPIDK GPPILAEDF LDIQGQPIN 
      PWSRIYPEE MIQTGISAI DVMNSIARG QKIPIFSAA 
      GLPHNEIAA QICRQAGLV KVPGKSVLD DHEDNFAIV 
      FAAMGVNME TARFFKQDF EENGSMENV CLFLNLAND 
      PTIERIITP RLALTAAEF MAYQCEKHV LVILTDMSS 
      YAEALREVS AAREEVPGR RGFPGYMYT DLATIYERA 
      GRVEGRNGS ITQIPILTM PNDDITHPI PDLTGYITE 
      GQIYVDRQL HNRQIYPPI NVLPSLSRL MKSAIGEGM 
      TRKDHSDVS NQLYACYAI GKDVQAMKA VVGEEALTP 
      DDLLYLEFL SKFEKNFIT QGSYENRTV FESLDIGWQ 
      LLRIFPKEM LKRIPAATL AEFYPRDSR H
    CDS sequence
    • >TC012133 RA
      atgtcttat caaaatagt atatcctca aaacaggcc 
      gctcgggag cacgttttg gcggtttcc cgtgatttc 
      gtgtctcaa ccaagactc acttataaa actgtaact 
      ggtgtaaat ggaccgttg gttattttg gacgacgtc 
      aagttcccc aaattcaac gaaatcgtc cagctcaag 
      ctgtccgat gggagcata cgttcgggg caagtgctg 
      gaagtcagc ggctccaaa gccgtcgtc caggtcttc 
      gagggcacg tcggggatc gacgccaaa cacaccgtg 
      tgcgagttc actggcgac atcctgagg acgcccgtg 
      tccgaggac atgctgggg cgcgtcttc aacggctcg 
      gggaagccc atagacaaa ggtccccca atcctcgcc 
      gaggacttc ctggacatc caggggcag cccatcaac 
      ccctggtcg cgtatctac cctgaagaa atgatccag 
      accggtatt tctgccatt gatgttatg aactcgatc 
      gctcgaggg cagaaaatc ccgattttc tctgccgcc 
      ggtctgccg cacaacgaa atcgccgcc caaatttgt 
      aggcaagct ggtcttgtt aaagtgccc gggaagtcc 
      gtactggat gaccatgaa gataatttc gctattgtt 
      ttcgccgct atgggtgtt aatatggaa actgcccga 
      tttttcaaa caagatttc gaagagaac ggctctatg 
      gagaatgta tgtttgttc ttgaacttg gccaatgat 
      ccaaccatt gagagaatt attacgcca cggttggcc 
      ctcacagcc gccgaattt atggcctac caatgcgag 
      aaacacgta cttgtgatc ttgaccgac atgtcttcg 
      tacgctgag gccttgcgt gaggtatcc gctgcccgt 
      gaagaggtg cccggccgt cgtggtttc cccggttac 
      atgtacacc gatttggcc acgatttac gagcgcgcc 
      ggtcgtgtc gagggcaga aacggctca atcacccaa 
      attcctatc cttactatg cctaacgac gatatcacc 
      catcctatt cccgacttg actggttac attaccgaa 
      ggacaaatc tacgtcgac cgtcagttg cacaacaga 
      cagatctat ccgccaatc aacgtgctg ccctccttg 
      tcgcgtctg atgaaatct gccatcggc gaaggaatg 
      accaggaag gaccactcc gacgtttcc aatcaattg 
      tatgcttgc tacgccatc ggtaaggac gtgcaagcc 
      atgaaggcc gtcgtgggt gaagaggcc ctcacgcct 
      gacgatctc ctgtatttg gagttcttg tccaagttc 
      gagaagaat ttcatcacg cagggaagt tacgagaac 
      cgcaccgtt ttcgagtcg ttggacatt ggctggcaa 
      ttgctccgc attttcccg aaggagatg ttgaagcgt 
      attcctgca gccaccttg gccgagttt tacccaagg 
      gactcacgc cattag
    mRNA sequence
    • >TC012133 RA
      agattttgg aaattggca aattgtgcg tggaacttt 
      cgattttcg tttgtagat tcctgcaag ttggggcgg 
      cacctttcg gtgggtacg cataattcg ccgtcacgt 
      gacgacacg tgaatccaa tacttgtgc agttgctga 
      ttttggttg ggctgtgga agacgtgtt gtgaaagat 
      gtcttatca aaatagtat atcctcaaa acaggccgc 
      tcgggagca cgttttggc ggtttcccg tgatttcgt 
      gtctcaacc aagactcac ttataaaac tgtaactgg 
      tgtaaatgg accgttggt tattttgga cgacgtcaa 
      gttccccaa attcaacga aatcgtcca gctcaagct 
      gtccgatgg gagcatacg ttcggggca agtgctgga 
      agtcagcgg ctccaaagc cgtcgtcca ggtcttcga 
      gggcacgtc ggggatcga cgccaaaca caccgtgtg 
      cgagttcac tggcgacat cctgaggac gcccgtgtc 
      cgaggacat gctggggcg cgtcttcaa cggctcggg 
      gaagcccat agacaaagg tcccccaat cctcgccga 
      ggacttcct ggacatcca ggggcagcc catcaaccc 
      ctggtcgcg tatctaccc tgaagaaat gatccagac 
      cggtatttc tgccattga tgttatgaa ctcgatcgc 
      tcgagggca gaaaatccc gattttctc tgccgccgg 
      tctgccgca caacgaaat cgccgccca aatttgtag 
      gcaagctgg tcttgttaa agtgcccgg gaagtccgt 
      actggatga ccatgaaga taatttcgc tattgtttt 
      cgccgctat gggtgttaa tatggaaac tgcccgatt 
      tttcaaaca agatttcga agagaacgg ctctatgga 
      gaatgtatg tttgttctt gaacttggc caatgatcc 
      aaccattga gagaattat tacgccacg gttggccct 
      cacagccgc cgaatttat ggcctacca atgcgagaa 
      acacgtact tgtgatctt gaccgacat gtcttcgta 
      cgctgaggc cttgcgtga ggtatccgc tgcccgtga 
      agaggtgcc cggccgtcg tggtttccc cggttacat 
      gtacaccga tttggccac gatttacga gcgcgccgg 
      tcgtgtcga gggcagaaa cggctcaat cacccaaat 
      tcctatcct tactatgcc taacgacga tatcaccca 
      tcctattcc cgacttgac tggttacat taccgaagg 
      acaaatcta cgtcgaccg tcagttgca caacagaca 
      gatctatcc gccaatcaa cgtgctgcc ctccttgtc 
      gcgtctgat gaaatctgc catcggcga aggaatgac 
      caggaagga ccactccga cgtttccaa tcaattgta 
      tgcttgcta cgccatcgg taaggacgt gcaagccat 
      gaaggccgt cgtgggtga agaggccct cacgcctga 
      cgatctcct gtatttgga gttcttgtc caagttcga 
      gaagaattt catcacgca gggaagtta cgagaaccg 
      caccgtttt cgagtcgtt ggacattgg ctggcaatt 
      gctccgcat tttcccgaa ggagatgtt gaagcgtat 
      tcctgcagc caccttggc cgagtttta cccaaggga 
      ctcacgcca ttagttttt gttacgatc ctgataggt 
      ttgttttaa aaatccact tatttttag ccatttctt 
      tgggccatt atggtgcaa ttttgtaaa taccaaaaa 
      acgtatcgt acttcattt ttccttcat ttgttgtat 
      attgtaaat tgaaattat tactcctta acaataatt 
      ttaaacagt agtacaaga caatatgta aagtaccac 
      gataggcat tgatattac ccttgttaa atatgaagc 
      acattttct ttgctaaga aatcaaagt ggaagcgcg 
      acattccat tactataat tcttgttat aaatgttgt 
      ataaagcaa tacaccaac cagctttca gtattttta 
      tttttagtt gtacattac gcggtgaat ggaaattgc 
      tagcattcg acaactctt tcggtgtta atatctata 
      agtaaacaa ccttatgtc tagactagt gtaaaaaat 
      gtgaataaa tgtatgatg ttacagttt tagattttg 
      tcatattct atgccttgt tttattaga atgaagtca 
      cgtgtttta tgtaaaata ttgttgaat aaatgctgt 
      acaaataga ctgtctttg actgcaccc cagtagctc 
      caaaccggg accggtagt tcaaatgtc gcaggcgga 
      gtaggcgga gccaaattt taattttga gttggtgtt 
      attattttt agcacagat tttggttat tgtgaatta 
      aatagtgga aaatttcaa gccaaactc aattagcac 
      aaattaatc gtggt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY