Status
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Gene information
General information
TC034296
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012129,TC012130
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5058617..5064566
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034296
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05179

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Pupal morphology
    head bristle size shortened - Number of animals showing the phenotype: 8

    wings not closed - Number of animals showing the phenotype: 4

    oblique dorsal thoracic muscles median size shortened - Number of animals showing the phenotype: 8
    oblique dorsal thoracic muscles thinner - Number of animals showing the phenotype: 8
    Comment: the outer oblique muscles are thinner, not the median ones
    longitudinal dorsal thoracic muscles fluorescence decreased - Number of animals showing the phenotype: 8

    abdomen gin-trap setae size shortened - Number of animals showing the phenotype: 8

    abdomen bristle size shortened - Number of animals showing the phenotype: 8
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034296 RA
      MNRFKLLFF VIIKISLYR VEAQISDKR LCVDEECKV 
      PISLAKTLL RYSSPDSRI LSFAPNVDI TIYSKSAGS 
      REDLWGGEI NGKRGYVPK HMVRETKVL QKPTLLVDT 
      EFSNETDSK EVPSQVEPN KVQEPYEVV DGTTIYLNP 
      QDDISPSST ESPIQSTLV PSQDDSSKF IGRTILSNE 
      PLQPEAGQK ESPKNEVNV ESTNQGNNL SESIEEQAK 
      SEEVSKEQI EIQTDPVPI SEENLSQKE APSSADENE 
      ESEEDDAEI DIDDENDDE DDEDEENVS TPETVVPAE 
      IKENVSSEN VEQKEEIQL GAESGSKMP ESEPPEVVT 
      ESKTEIVEP KTIVASIEE EKHDSKAEE NLESLKEET 
      VPNTEAPTL APNEPLALT EEEKNVPGA EENLESPKK 
      EVIPPGIEA TTLSPEIEV PQPFDDSIV DVTPNPVEP 
      VPEEPKISM DGIPTLPPF GLFGSPNPE PVQNAEVAP 
      EPEKTDLGL NTPLEETQK ELQFQKLEE IPVQNIEEK 
      IEETQPQNS EEPIEVTPQ SIEEKMPQN SEEKIEEIR 
      PQTSEETIE EIPQNSEEK IDEVTENSQ VEENDNPGF 
      FSGILAFMG LGSQENTEE ERIMEQFIP SEEHMPSDD 
      VISKVREES FCESGSCPK NELSDDAME AFGFDLFNF 
      NSNILLYLV TTAISVIIF LFGYIALDK SKREGPLIA 
      KINSLEKEL LIIIKENQI LQEKLSTDS GYETNDRVP 
      SEVVENLKQ DLLAVTNVK NALEEQVQS LEKELENST 
      EVGMELNRM LTEILSSEG GSDVLKANV ENLQRQLVE 
      QQATINSVN ETLAVTNTE NHELRLELE LSNKKVLDL 
      QAELDKLLL NILKIEEEK DTQQSTLEN EIANYRQKF 
      EQVSNKLKL MENDHSLEI KQLRNQLDQ TERKLELKT 
      KEYDQLKDT LKQVKSLKN NESTLRDLL EVTDIKAEL 
      EVLKSENER FSQKLRQEQ DLKSTFEKK AQSAEDELR 
      VLREKYDET DKLRVESQT KLEVLSNYF KDREAQWQK 
      EISKHEALW AEKEGEASS TSERIKFMQ EQLQNYKAQ 
      TESLKQEIM SQEIELKSQ ISVLEKKAH ENWVSARQA 
      ERKLEEAKQ EAAQLRHRL TLQERDKRL QSPLEQNGD 
      IPESPASPP LLFGTRDHI TKSPPLPGL PPFLPPPPG 
      VPFMPPPLP GVPPFIPPP PPNMFPGDH RPPPLGRMS 
      SPPLNSRYS PSSRGYSPY DRNSSSPSD SEYGTSPPH 
      RRYSPFRGD DRRDYKRPP PGRTNGRSM MKGGSSGSG 
      HSNESLEKI NRQQSKV
    CDS sequence
    • >TC034296 RA
      atgaataga tttaagtta ttatttttt gtgataatt 
      aaaataagc ttgtataga gtagaagcc caaatatcc 
      gataagaga ttatgcgtg gacgaagaa tgtaaagtc 
      cccatttcg cttgccaag actcttttg aggtattct 
      agtcccgac tcgaggatc ctgtcgttc gcccccaac 
      gtcgacata acgatttac agtaaatca gctggttct 
      cgagaagac ctttggggg ggcgagatt aatggcaaa 
      aggggatat gtccccaaa catatggtc cgagaaacg 
      aaagttctt caaaagccg actctttta gtcgacacc 
      gagttcagt aatgagacc gattcaaaa gaagtgcct 
      tcacaagtt gaaccaaac aaagttcag gaaccttac 
      gaagttgtt gatggtacg accatttac ctaaatccg 
      caagatgat atcagtccg tcgtccacc gagagccca 
      atccaatca acacttgta ccatcacaa gacgactct 
      agcaaattt attgggagg accattttg tctaatgag 
      cctttacag ccagaagct ggacaaaag gaatctccc 
      aaaaacgaa gtgaacgtc gaaagcaca aatcaagga 
      aataattta agcgaatct atagaagaa caagccaaa 
      agtgaagaa gtttcaaag gagcaaata gaaatacaa 
      acggatcca gtaccaatt agtgaagaa aatttgtca 
      cagaaagaa gcaccttca agtgcagat gaaaacgaa 
      gagtccgaa gaagatgat gccgagatc gacatagat 
      gacgaaaat gatgatgag gatgatgaa gatgaagaa 
      aatgtatca actccagaa actgtagtt ccagctgaa 
      attaaggaa aacgtttct agtgaaaat gtagaacag 
      aaggaagaa attcaatta ggagcagaa agtgggtca 
      aagatgcca gaatctgaa ccaccagaa gtggtgaca 
      gaatcaaaa acagaaatt gtagagcct aaaactatt 
      gttgcatca attgaagag gaaaaacat gattctaaa 
      gcggaggaa aacttggaa agtctaaag gaagaaaca 
      gttcctaat actgaagcc cctactttg gctccaaac 
      gagcctctg gcactcact gaagaagaa aaaaatgtt 
      cccggagcg gaagaaaac cttgaaagt ccaaagaaa 
      gaagtaatt cctcctggt attgaagcc accactttg 
      agtccagaa atagaagtg cctcaaccc tttgatgac 
      agtattgtt gatgtaact ccaaaccct gtcgaaccg 
      gttccagaa gagcctaaa atttcaatg gatggaatc 
      ccaacactg ccaccgttt ggacttttc ggaagtcca 
      aatccagaa ccagtccag aacgcagaa gtagctcca 
      gaaccggaa aaaactgat ttgggattg aacacacca 
      ttggaagaa acgcaaaaa gaactacag ttccagaaa 
      cttgaagaa atcccagtc caaaatatt gaagagaaa 
      attgaagaa actcaaccg cagaatagc gaagagccg 
      attgaagta acaccccaa agtattgaa gagaaaatg 
      ccccaaaac agtgaagag aaaattgaa gaaattcgg 
      ccgcagact agcgaagag acgattgaa gaaatacca 
      caaaatagt gaagagaaa atcgatgaa gtgacggag 
      aatagtcaa gttgaagaa aatgacaat cctgggttt 
      ttctcggga atattggct tttatgggg ttgggttcg 
      caagaaaac acggaggag gaacgcata atggaacaa 
      tttattcct tccgaagaa catatgcca agtgatgat 
      gtaatttcc aaagtgcgt gaagaatcg ttttgtgag 
      tctggttct tgtccgaaa aatgagcta agtgacgat 
      gccatggag gcgttcggt ttcgacttg ttcaacttc 
      aactcaaat atactcctc tatttggta acaacagcg 
      atttctgtt attattttc ttgtttggc tacattgcg 
      cttgataag agtaaacga gaaggcccg ctcattgct 
      aaaattaat agtctcgag aaagaattg ttgataata 
      atcaaagaa aaccagatt ttacaagaa aagttatcg 
      actgattcc ggttacgaa accaatgat cgcgtacca 
      agcgaagtc gttgaaaac ttgaaacag gaccttttg 
      gccgtgaca aacgtcaaa aatgccctc gaggaacag 
      gtgcagtcg ctggagaag gaactggaa aattcgacc 
      gaagtcgga atggaactg aatcgaatg ctgacggaa 
      attttaagt tccgagggc ggtagcgat gtccttaag 
      gccaacgtt gagaattta caacggcaa cttgtcgag 
      caacaagcc accattaat tcagttaac gaaacttta 
      gctgttacg aataccgag aatcacgaa cttcggttg 
      gagctggaa ttgagcaat aagaaagtt ttagattta 
      caagccgag ttggataag ttattactg aatattctc 
      aaaatcgaa gaagagaag gacactcaa cagagtacg 
      ctggaaaat gaaatagcc aactacagg cagaaattc 
      gaacaagtt agcaacaaa ctcaaactg atggagaat 
      gaccacagt ctggaaata aaacagttg agaaatcaa 
      ttagaccaa actgagcgt aaactagaa ctgaaaaca 
      aaggaatac gaccaactt aaagacact ttaaagcaa 
      gtcaagtct ctcaaaaac aacgaatcg acgttacgc 
      gacctactt gaagtgact gacatcaag gccgaactg 
      gaggtcctc aagtcggag aatgaacgg ttcagccaa 
      aagttgcga caggaacaa gacctgaaa tcaaccttt 
      gagaagaag gcacagagc gccgaagac gagctccgg 
      gtcctaaga gagaaatac gacgaaacc gacaagttg 
      cgcgtcgag agtcagacc aaactcgag gtcttgtcc 
      aactacttc aaagacagg gaagcacaa tggcagaag 
      gaaatcagc aagcatgag gcgctttgg gccgagaaa 
      gaaggagaa gcttcctca accagcgaa cgaattaaa 
      ttcatgcag gaacaatta cagaattac aaggcccag 
      accgagtca cttaaacaa gaaataatg tctcaagaa 
      attgaactg aagagtcaa atttcggtt ttggagaag 
      aaagcgcac gaaaattgg gtgagtgcc cgacaagct 
      gaacggaag ctcgaagaa gcgaaacaa gaggcggca 
      caactgcga catagacta acgttacaa gaacgagat 
      aaaaggttg caatcgcca cttgagcaa aacggggac 
      atccccgaa tccccggcc tcccctcct cttttattt 
      ggcacgcgt gaccacatt accaaatct cctcccctt 
      ccagggctt cctcccttc ttgccgccc cctcccgga 
      gttcccttc atgccgccg ccacttccg ggtgtcccc 
      ccttttatc ccgcccccg ccacctaac atgtttccg 
      ggtgatcac cgcccccct ccactagga aggatgtcg 
      tcccctcca ctaaattcc cgatattcg ccgagtagt 
      cggggatat tcgccttac gatcggaac agttcgtcg 
      ccttccgat tccgaatac ggtacttcc cctccacat 
      cgcaggtac agtccgttc agaggggat gataggcgg 
      gattacaaa aggcctccc ccggggagg accaatggg 
      aggagtatg atgaaaggt ggtagttca gggtcggga 
      cattcgaac gaatcgtta gagaagata aatagacag 
      cagtcgaaa gtctag
    mRNA sequence
    • >TC034296 RA
      cagggatag agggcgctt cacttttca aaagagtga 
      tgtggcttt gcgatcgac ttgtcaaag tatgacaaa 
      atgaataga tttaagtta ttatttttt gtgataatt 
      aaaataagc ttgtataga gtagaagcc caaatatcc 
      gataagaga ttatgcgtg gacgaagaa tgtaaagtc 
      cccatttcg cttgccaag actcttttg aggtattct 
      agtcccgac tcgaggatc ctgtcgttc gcccccaac 
      gtcgacata acgatttac agtaaatca gctggttct 
      cgagaagac ctttggggg ggcgagatt aatggcaaa 
      aggggatat gtccccaaa catatggtc cgagaaacg 
      aaagttctt caaaagccg actctttta gtcgacacc 
      gagttcagt aatgagacc gattcaaaa gaagtgcct 
      tcacaagtt gaaccaaac aaagttcag gaaccttac 
      gaagttgtt gatggtacg accatttac ctaaatccg 
      caagatgat atcagtccg tcgtccacc gagagccca 
      atccaatca acacttgta ccatcacaa gacgactct 
      agcaaattt attgggagg accattttg tctaatgag 
      cctttacag ccagaagct ggacaaaag gaatctccc 
      aaaaacgaa gtgaacgtc gaaagcaca aatcaagga 
      aataattta agcgaatct atagaagaa caagccaaa 
      agtgaagaa gtttcaaag gagcaaata gaaatacaa 
      acggatcca gtaccaatt agtgaagaa aatttgtca 
      cagaaagaa gcaccttca agtgcagat gaaaacgaa 
      gagtccgaa gaagatgat gccgagatc gacatagat 
      gacgaaaat gatgatgag gatgatgaa gatgaagaa 
      aatgtatca actccagaa actgtagtt ccagctgaa 
      attaaggaa aacgtttct agtgaaaat gtagaacag 
      aaggaagaa attcaatta ggagcagaa agtgggtca 
      aagatgcca gaatctgaa ccaccagaa gtggtgaca 
      gaatcaaaa acagaaatt gtagagcct aaaactatt 
      gttgcatca attgaagag gaaaaacat gattctaaa 
      gcggaggaa aacttggaa agtctaaag gaagaaaca 
      gttcctaat actgaagcc cctactttg gctccaaac 
      gagcctctg gcactcact gaagaagaa aaaaatgtt 
      cccggagcg gaagaaaac cttgaaagt ccaaagaaa 
      gaagtaatt cctcctggt attgaagcc accactttg 
      agtccagaa atagaagtg cctcaaccc tttgatgac 
      agtattgtt gatgtaact ccaaaccct gtcgaaccg 
      gttccagaa gagcctaaa atttcaatg gatggaatc 
      ccaacactg ccaccgttt ggacttttc ggaagtcca 
      aatccagaa ccagtccag aacgcagaa gtagctcca 
      gaaccggaa aaaactgat ttgggattg aacacacca 
      ttggaagaa acgcaaaaa gaactacag ttccagaaa 
      cttgaagaa atcccagtc caaaatatt gaagagaaa 
      attgaagaa actcaaccg cagaatagc gaagagccg 
      attgaagta acaccccaa agtattgaa gagaaaatg 
      ccccaaaac agtgaagag aaaattgaa gaaattcgg 
      ccgcagact agcgaagag acgattgaa gaaatacca 
      caaaatagt gaagagaaa atcgatgaa gtgacggag 
      aatagtcaa gttgaagaa aatgacaat cctgggttt 
      ttctcggga atattggct tttatgggg ttgggttcg 
      caagaaaac acggaggag gaacgcata atggaacaa 
      tttattcct tccgaagaa catatgcca agtgatgat 
      gtaatttcc aaagtgcgt gaagaatcg ttttgtgag 
      tctggttct tgtccgaaa aatgagcta agtgacgat 
      gccatggag gcgttcggt ttcgacttg ttcaacttc 
      aactcaaat atactcctc tatttggta acaacagcg 
      atttctgtt attattttc ttgtttggc tacattgcg 
      cttgataag agtaaacga gaaggcccg ctcattgct 
      aaaattaat agtctcgag aaagaattg ttgataata 
      atcaaagaa aaccagatt ttacaagaa aagttatcg 
      actgattcc ggttacgaa accaatgat cgcgtacca 
      agcgaagtc gttgaaaac ttgaaacag gaccttttg 
      gccgtgaca aacgtcaaa aatgccctc gaggaacag 
      gtgcagtcg ctggagaag gaactggaa aattcgacc 
      gaagtcgga atggaactg aatcgaatg ctgacggaa 
      attttaagt tccgagggc ggtagcgat gtccttaag 
      gccaacgtt gagaattta caacggcaa cttgtcgag 
      caacaagcc accattaat tcagttaac gaaacttta 
      gctgttacg aataccgag aatcacgaa cttcggttg 
      gagctggaa ttgagcaat aagaaagtt ttagattta 
      caagccgag ttggataag ttattactg aatattctc 
      aaaatcgaa gaagagaag gacactcaa cagagtacg 
      ctggaaaat gaaatagcc aactacagg cagaaattc 
      gaacaagtt agcaacaaa ctcaaactg atggagaat 
      gaccacagt ctggaaata aaacagttg agaaatcaa 
      ttagaccaa actgagcgt aaactagaa ctgaaaaca 
      aaggaatac gaccaactt aaagacact ttaaagcaa 
      gtcaagtct ctcaaaaac aacgaatcg acgttacgc 
      gacctactt gaagtgact gacatcaag gccgaactg 
      gaggtcctc aagtcggag aatgaacgg ttcagccaa 
      aagttgcga caggaacaa gacctgaaa tcaaccttt 
      gagaagaag gcacagagc gccgaagac gagctccgg 
      gtcctaaga gagaaatac gacgaaacc gacaagttg 
      cgcgtcgag agtcagacc aaactcgag gtcttgtcc 
      aactacttc aaagacagg gaagcacaa tggcagaag 
      gaaatcagc aagcatgag gcgctttgg gccgagaaa 
      gaaggagaa gcttcctca accagcgaa cgaattaaa 
      ttcatgcag gaacaatta cagaattac aaggcccag 
      accgagtca cttaaacaa gaaataatg tctcaagaa 
      attgaactg aagagtcaa atttcggtt ttggagaag 
      aaagcgcac gaaaattgg gtgagtgcc cgacaagct 
      gaacggaag ctcgaagaa gcgaaacaa gaggcggca 
      caactgcga catagacta acgttacaa gaacgagat 
      aaaaggttg caatcgcca cttgagcaa aacggggac 
      atccccgaa tccccggcc tcccctcct cttttattt 
      ggcacgcgt gaccacatt accaaatct cctcccctt 
      ccagggctt cctcccttc ttgccgccc cctcccgga 
      gttcccttc atgccgccg ccacttccg ggtgtcccc 
      ccttttatc ccgcccccg ccacctaac atgtttccg 
      ggtgatcac cgcccccct ccactagga aggatgtcg 
      tcccctcca ctaaattcc cgatattcg ccgagtagt 
      cggggatat tcgccttac gatcggaac agttcgtcg 
      ccttccgat tccgaatac ggtacttcc cctccacat 
      cgcaggtac agtccgttc agaggggat gataggcgg 
      gattacaaa aggcctccc ccggggagg accaatggg 
      aggagtatg atgaaaggt ggtagttca gggtcggga 
      cattcgaac gaatcgtta gagaagata aatagacag 
      cagtcgaaa gtctagcgg ttcacctgt tgtttttat 
      tattagttt ttgtgtttg aaattttca gcggtccaa 
      atttttcgt cgcgtaggt gaactgaag tgaatagtg 
      ctttgtaat aatctctgg tgtaatacg tgacaagat 
      tctgtctac tctaacatt tttgaattt gtttttaaa 
      aagtatgta taaacttgt ataaagtac tgttgtata 
      ataaaatgt ttatggaaa a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY