Status
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Gene information
General information
TC034299
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012125
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5204663..5209936
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034299
    iBeetle screen iB_05178

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034299 RA
      MLFRRFKNC IKLNKYQTR TCCSSVEEP KGPSVKTPI 
      PGPNSKKLQ GELHKIHYS ETIQLFINY VKSIGNYLV 
      DADGNVILD TYTQISAAP LGYNHPELL KVFKNDDNL 
      KRLINRPAL GLFPGDDYP ERVKNVFSQ IAPCLPKIY 
      LMMCGACAN ENAFKHMFV SYQLKKRGG RPFTEEEKT 
      SAVLNKPPG CPNLTILSF HNAFLGRTF ACLAATHSK 
      FIQKVDLPS IDWPAAHFP IYKYPLEEN MRENKEEDR 
      KCLAEVEDL MAKYEKKGT PVAGVVVEP LQSEAGNYE 
      ASPKFFQQL QKIVKKNNA YLLFDEVQT GGGASGKFW 
      FHEHFDLET PPDVITFAK KFQLGGYFH SEELTPDVP 
      YRIFNTWMG DPGKLMLLE VIIKVMREQ NLLETVQKA 
      GKRLKCGLH QFESEFPNL VYSVRGVGT LLAFDAKDE 
      NMRNEFMKK LLNKGVLVG NCGYKGIRF RPALTFDES 
      HVDIFLDKF REVLKEINN DCVRNKRVN TYFGESRAV 
      VNMQLWSKT VSPILQNVA KTSSVRTYV KGEPTGPQL 
      KTEIPGPNS RKLHAELNA IQQSEAVQF FADYNKSLG 
      NYIVDVDGN VILDTYNQI ASIPIGYNH PSLLKVFQN 
      EDNLKALVN RPALGVFPG DYWPKKLRS VLLSVAPRL 
      PNVTTMMCG SCSNENAYK AMFIAYRRH QRGENVDFT 
      DLEKESCVS NKPPGAPDL SLLSFHGAF HGRTLGALA 
      TTHSKAIHK LDVPSFDWP IAPFPQYKY PLEEHAREN 
      AAEDKRCLA AVEDLIQTY NKKGKPVAG IVIEPIQSE 
      GGDNEASPE FFQQLQKIA KKYGASYLI DEVQTGCGA 
      TGKFWAHEH FNLESPPDI VTFSKKMLT GGYYHSVDL 
      RPKQPYRIF NTWMGDPGK IALLEACLE TIKNENLLD 
      NVNKVGDKL KKGLLRLEA EFPEKINST RGRGTFLAV 
      NAHTPALRD SIIAKMKQK GVLMGGCGD HSLRLRPSL 
      IFQERHADV FLERFRQVL REN
    CDS sequence
    • >TC034299 RA
      atgttattc agacgtttt aaaaactgc ataaaactg 
      aacaagtat caaactcgt acatgttgt tcttcggta 
      gaagaaccc aaaggccct tctgtcaaa acgccaata 
      cctggcccc aattccaaa aaactccaa ggagagttg 
      cacaaaata cattattca gaaacaatc cagctgttt 
      attaattac gtaaaatca attggaaat tatttggtc 
      gacgccgac ggcaatgtt atcttggac acctacacc 
      caaatatcc gcagccccc ttaggctac aaccacccc 
      gaattactc aaagtgttc aaaaacgac gacaatctt 
      aaacgttta attaacaga cccgctctt gggttattc 
      cccggagac gattatccc gaaagagtg aaaaatgtg 
      ttcagtcaa attgcgccc tgtttgccc aaaatctac 
      ctcatgatg tgcggggcg tgtgccaac gaaaacgcc 
      ttcaaacac atgtttgtg agctaccag ctcaaaaaa 
      cgaggcggc cgccccttt accgaagaa gagaaaact 
      tccgccgtg cttaacaaa cccccaggt tgtcccaac 
      ttaacaata ctgtcgttc cacaacgct tttctcggc 
      cggactttt gcctgtttg gccgcaacg cactcgaaa 
      tttatccaa aaggttgac ctcccgtcc atcgattgg 
      cccgctgcg cattttcca atatacaag tatccttta 
      gaggaaaat atgcgggaa aataaggaa gaagacagg 
      aaatgtttg gccgaagtt gaggacttg atggcgaag 
      tatgagaag aaaggaaca ccggttgct ggagtagtc 
      gtcgagcct ttacaatca gaagcgggg aattacgaa 
      gcatccccg aaatttttc caacaatta caaaaaatc 
      gtgaagaag aacaacgcg tatttgttg ttcgatgaa 
      gtgcaaacg gggggcgga gcctcgggg aagttttgg 
      tttcacgaa cattttgac ctagagacg ccgcccgat 
      gtaatcact tttgcgaaa aaatttcaa cttgggggt 
      tacttccat tcggaggag ttgacgccg gatgtgcct 
      tatcgtatt ttcaacacc tggatggga gatccgggg 
      aaacttatg ttacttgaa gtgattatt aaagtcatg 
      agggagcaa aatctttta gagactgta caaaaagcc 
      ggaaaaaga ctcaagtgt ggtctccac cagttcgaa 
      agtgaattt cctaattta gtgtattca gtacgaggg 
      gttggaact cttcttgcc ttcgatgcc aaagatgaa 
      aacatgaga aatgaattt atgaagaaa ctgctaaat 
      aaaggtgtc ctggttggg aattgtggt tacaaaggc 
      attaggttt cggcccgct ttaactttc gacgaaagc 
      cacgtcgat atattttta gacaagttc agagaagta 
      ttgaaagaa attaataat gattgtgta cgtaacaag 
      agagtgaac acgtatttt ggtgagagc agagcggtt 
      gtaaacatg cagttgtgg tcgaaaaca gtgagtccc 
      atccttcaa aatgtcgca aaaacgagc tctgttagg 
      acttacgtc aaaggcgaa ccgacgggg ccccagcta 
      aaaaccgaa attccgggt ccgaactcc cgaaaactt 
      cacgccgaa ctcaatgcc atccaacag agcgaagcg 
      gttcagttt ttcgccgat tacaacaaa tccctcggg 
      aactacatc gtggatgtt gacgggaac gtaatcctc 
      gacacatac aaccaaatc gcgtcaatc ccgatagga 
      tacaaccat ccatcccta ctcaaagta ttccaaaat 
      gaagataat ttgaaagca ctagtcaac cggccagcc 
      ctgggcgta ttcccgggc gattactgg ccgaaaaag 
      ctccgcagc gtattactc tcagtggcc ccccggctc 
      ccaaacgtc accacaatg atgtgtggc tcgtgttcg 
      aacgaaaac gcgtacaaa gccatgttt atcgcctac 
      cgccggcac caacgaggc gaaaacgtc gatttcacc 
      gacctggag aaagagagc tgcgttagt aacaagcca 
      cccggcgcc cccgacctg tccctgctg tccttccat 
      ggggccttc cacggccgc actttgggg gccctcgcc 
      accacgcac tccaaagcc atccacaag ctcgacgtg 
      ccctcgttc gactggccc atcgccccc ttcccacag 
      tacaagtac ccccttgag gaacacgcg cgcgagaac 
      gcggccgaa gacaagagg tgtctcgca gcggtcgag 
      gacttgatc cagacttac aataagaag ggcaaaccc 
      gtggcgggg atcgtcatc gagcccatc cagtcggaa 
      gggggcgac aacgaggcc tcgccggaa ttcttccaa 
      cagttgcag aaaatcgcc aagaaatac ggcgccagt 
      tatctcatt gacgaagtc cagacaggg tgtggggcc 
      acagggaag ttctgggca cacgaacac ttcaatcta 
      gaatcgccc cctgatatc gtcaccttc agcaaaaaa 
      atgctgaca ggggggtac taccattcg gttgatttg 
      aggcccaaa cagccctac aggattttc aatacctgg 
      atgggggat ccggggaaa atcgccctg ttggaggcg 
      tgtcttgag actatcaaa aatgagaat ctcctggat 
      aatgttaat aaagtcggg gataagttg aagaaaggc 
      ttgttgaga ctggaggct gaatttcct gaaaaaatt 
      aactccacc aggggacgt ggcacgttt ttggccgtg 
      aatgcgcac acgcccgct ttgcgagac agtattatt 
      gcgaaaatg aagcagaaa ggtgttttg atggggggc 
      tgtggggat cactctttg aggctgaga ccgtcgctc 
      attttccag gaacgccac gccgatgtg ttcctggag 
      cgcttcaga caggtcctg agggaaaac taa
    mRNA sequence
    • >TC034299 RA
      aaacggacg caaaaatgt tattcagac gttttaaaa 
      actgcataa aactgaaca agtatcaaa ctcgtacat 
      gttgttctt cggtagaag aacccaaag gcccttctg 
      tcaaaacgc caatacctg gccccaatt ccaaaaaac 
      tccaaggag agttgcaca aaatacatt attcagaaa 
      caatccagc tgtttatta attacgtaa aatcaattg 
      gaaattatt tggtcgacg ccgacggca atgttatct 
      tggacacct acacccaaa tatccgcag cccccttag 
      gctacaacc accccgaat tactcaaag tgttcaaaa 
      acgacgaca atcttaaac gtttaatta acagacccg 
      ctcttgggt tattccccg gagacgatt atcccgaaa 
      gagtgaaaa atgtgttca gtcaaattg cgccctgtt 
      tgcccaaaa tctacctca tgatgtgcg gggcgtgtg 
      ccaacgaaa acgccttca aacacatgt ttgtgagct 
      accagctca aaaaacgag gcggccgcc cctttaccg 
      aagaagaga aaacttccg ccgtgctta acaaacccc 
      caggttgtc ccaacttaa caatactgt cgttccaca 
      acgcttttc tcggccgga cttttgcct gtttggccg 
      caacgcact cgaaattta tccaaaagg ttgacctcc 
      cgtccatcg attggcccg ctgcgcatt ttccaatat 
      acaagtatc ctttagagg aaaatatgc gggaaaata 
      aggaagaag acaggaaat gtttggccg aagttgagg 
      acttgatgg cgaagtatg agaagaaag gaacaccgg 
      ttgctggag tagtcgtcg agcctttac aatcagaag 
      cggggaatt acgaagcat ccccgaaat ttttccaac 
      aattacaaa aaatcgtga agaagaaca acgcgtatt 
      tgttgttcg atgaagtgc aaacggggg gcggagcct 
      cggggaagt tttggtttc acgaacatt ttgacctag 
      agacgccgc ccgatgtaa tcacttttg cgaaaaaat 
      ttcaacttg ggggttact tccattcgg aggagttga 
      cgccggatg tgccttatc gtattttca acacctgga 
      tgggagatc cggggaaac ttatgttac ttgaagtga 
      ttattaaag tcatgaggg agcaaaatc ttttagaga 
      ctgtacaaa aagccggaa aaagactca agtgtggtc 
      tccaccagt tcgaaagtg aatttccta atttagtgt 
      attcagtac gaggggttg gaactcttc ttgccttcg 
      atgccaaag atgaaaaca tgagaaatg aatttatga 
      agaaactgc taaataaag gtgtcctgg ttgggaatt 
      gtggttaca aaggcatta ggtttcggc ccgctttaa 
      ctttcgacg aaagccacg tcgatatat ttttagaca 
      agttcagag aagtattga aagaaatta ataatgatt 
      gtgtacgta acaagagag tgaacacgt attttggtg 
      agagcagag cggttgtaa acatgcagt tgtggtcga 
      aaacagtga gtcccatcc ttcaaaatg tcgcaaaaa 
      cgagctctg ttaggactt acgtcaaag gcgaaccga 
      cggggcccc agctaaaaa ccgaaattc cgggtccga 
      actcccgaa aacttcacg ccgaactca atgccatcc 
      aacagagcg aagcggttc agtttttcg ccgattaca 
      acaaatccc tcgggaact acatcgtgg atgttgacg 
      ggaacgtaa tcctcgaca catacaacc aaatcgcgt 
      caatcccga taggataca accatccat ccctactca 
      aagtattcc aaaatgaag ataatttga aagcactag 
      tcaaccggc cagccctgg gcgtattcc cgggcgatt 
      actggccga aaaagctcc gcagcgtat tactctcag 
      tggcccccc ggctcccaa acgtcacca caatgatgt 
      gtggctcgt gttcgaacg aaaacgcgt acaaagcca 
      tgtttatcg cctaccgcc ggcaccaac gaggcgaaa 
      acgtcgatt tcaccgacc tggagaaag agagctgcg 
      ttagtaaca agccacccg gcgcccccg acctgtccc 
      tgctgtcct tccatgggg ccttccacg gccgcactt 
      tgggggccc tcgccacca cgcactcca aagccatcc 
      acaagctcg acgtgccct cgttcgact ggcccatcg 
      cccccttcc cacagtaca agtaccccc ttgaggaac 
      acgcgcgcg agaacgcgg ccgaagaca agaggtgtc 
      tcgcagcgg tcgaggact tgatccaga cttacaata 
      agaagggca aacccgtgg cggggatcg tcatcgagc 
      ccatccagt cggaagggg gcgacaacg aggcctcgc 
      cggaattct tccaacagt tgcagaaaa tcgccaaga 
      aatacggcg ccagttatc tcattgacg aagtccaga 
      cagggtgtg gggccacag ggaagttct gggcacacg 
      aacacttca atctagaat cgccccctg atatcgtca 
      ccttcagca aaaaaatgc tgacagggg ggtactacc 
      attcggttg atttgaggc ccaaacagc cctacagga 
      ttttcaata cctggatgg gggatccgg ggaaaatcg 
      ccctgttgg aggcgtgtc ttgagacta tcaaaaatg 
      agaatctcc tggataatg ttaataaag tcggggata 
      agttgaaga aaggcttgt tgagactgg aggctgaat 
      ttcctgaaa aaattaact ccaccaggg gacgtggca 
      cgtttttgg ccgtgaatg cgcacacgc ccgctttgc 
      gagacagta ttattgcga aaatgaagc agaaaggtg 
      ttttgatgg ggggctgtg gggatcact ctttgaggc 
      tgagaccgt cgctcattt tccaggaac gccacgccg 
      atgtgttcc tggagcgct tcagacagg tcctgaggg 
      aaaactaat gtaataatt aaacaaaat aaaaattat 
      acttgcgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY