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Gene information
General information
TC034301 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5285036..5339573
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034301
    iBeetle screen iB_05177

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034301 RA
      MSNESSSLR DPPLTTNSS VEDSTTHIQ KLALAGLLS 
      VVIVVVIIG NTLVILAVI TTKRLRTLG WVLCDIWVS 
      LDILLCTAS ILSLCAISV DRYLAVTQP LSYSRRRRS 
      KRLAFTMIL VVWMSSALI TCPPMFGWY EPGRHEEKN 
      CRYNKNTGY VIFSAMGSF FIPMMVMLY VYVRISCVV 
      AQRHDQLAH IDTHSRKSQ KLVISVKEE SDIDRVSSE 
      CEDQNLRCP RYFRQISDA CGQSAEPLN QSLNHEQRK 
      SLGTRSLRS PLNYSFNHE PLTAQRSAS LYRPQPFTP 
      QRSRPESLN FEPATRVSS FRRETKTAQ TLSIVMGGF 
      IACWLPFFI FYLLTPFIP SNQVNSLIM SYLTWLGWF 
      NSAINPFIY AFYSPDFRI AFWRLTVKH FSKTRRPTS 
      PFSTSEGRR RTRSILVTY FIVVIYIVL L
    CDS sequence
    • >TC034301 RA
      atgtcgaac gagtcgtct agcctgcgc gacccccca 
      ttgacgacc aactccagc gtggaggac tccaccacc 
      cacatccaa aagctggcc ctcgccggc ctcctcagc 
      gtggtcatc gtcgtggtg atcatcggc aacactctc 
      gttattctg gctgtgata acaacaaag cgattacgc 
      acactcggc tgggtgctg tgcgacatc tgggtctcg 
      ctcgatatc ctcctgtgc acggcatcg atcctgagc 
      ctgtgtgcc atcagcgtg gaccgatac ctggccgtg 
      actcagccc ctgagctac tccaggcga aggcgctcc 
      aagaggttg gccttcacc atgatcctg gtggtgtgg 
      atgagctcg gcgcttatc acctgtccg cccatgttt 
      ggatggtac gagccagga aggcacgag gagaagaac 
      tgcaggtac aataagaac acgggctat gtgatcttt 
      tcagcgatg ggctcgttc ttcataccc atgatggtc 
      atgctctac gtctacgtg cggatatcc tgtgtggtg 
      gcccagagg cacgaccag ctcgcacac atcgacacc 
      cattcgagg aaaagccaa aaactcgtc atcagcgtt 
      aaggaagag tctgacatt gatcgagtt tcctctgaa 
      tgtgaggac caaaacttg agatgtccc aggtatttt 
      cgtcagata agtgatgca tgcgggcag tcggcggag 
      cccctcaac cagagcctc aaccacgag cagcgtaaa 
      tctctcggc actcgttcg ctccgctcg ccgttgaac 
      tactccttc aatcacgag cctctgacg gcgcagcgc 
      tccgccagc ctctaccgg ccgcagccg ttcacgcca 
      cagcgctcc cggcccgag tccctcaac ttcgagccc 
      gcgacgcgc gtctcctcc ttccgccgc gagaccaag 
      acggcacag acgctcagc atcgtcatg ggcggcttc 
      atcgcctgc tggctgccc ttcttcatt ttctacctg 
      ctgacgccg ttcattccc agcaaccag gtcaactcg 
      ctgatcatg tcctacttg acgtggctg ggctggttc 
      aactcggcc atcaacccc ttcatctac gccttctac 
      agccccgac ttcaggata gccttctgg cgactgact 
      gttaagcat ttttcaaag acacgaagg ccgacttcg 
      cctttcagc acaagtgag ggccggcgc cggacacgt 
      tcgattcta gtcacatat tttatagtt gttatttat 
      attgtacta ttgtaa
    mRNA sequence
    • >TC034301 RA
      ccgcataat cccgcctta aacccgatt aagctctag 
      caaaaatga aacaaaccc ctgtaaaaa atctttcag 
      ccaaattaa tgtgttgtt tctgttcca gcctcggga 
      atccgatta aatctaatc gccgaaaag aaataaatg 
      actaagaat gtcgaacga gtcgtctag cctgcgcga 
      ccccccatt gacgaccaa ctccagcgt ggaggactc 
      caccaccca catccaaaa gctggccct cgccggcct 
      cctcagcgt ggtcatcgt cgtggtgat catcggcaa 
      cactctcgt tattctggc tgtgataac aacaaagcg 
      attacgcac actcggctg ggtgctgtg cgacatctg 
      ggtctcgct cgatatcct cctgtgcac ggcatcgat 
      cctgagcct gtgtgccat cagcgtgga ccgatacct 
      ggccgtgac tcagcccct gagctactc caggcgaag 
      gcgctccaa gaggttggc cttcaccat gatcctggt 
      ggtgtggat gagctcggc gcttatcac ctgtccgcc 
      catgtttgg atggtacga gccaggaag gcacgagga 
      gaagaactg caggtacaa taagaacac gggctatgt 
      gatcttttc agcgatggg ctcgttctt catacccat 
      gatggtcat gctctacgt ctacgtgcg gatatcctg 
      tgtggtggc ccagaggca cgaccagct cgcacacat 
      cgacaccca ttcgaggaa aagccaaaa actcgtcat 
      cagcgttaa ggaagagtc tgacattga tcgagtttc 
      ctctgaatg tgaggacca aaacttgag atgtcccag 
      gtattttcg tcagataag tgatgcatg cgggcagtc 
      ggcggagcc cctcaacca gagcctcaa ccacgagca 
      gcgtaaatc tctcggcac tcgttcgct ccgctcgcc 
      gttgaacta ctccttcaa tcacgagcc tctgacggc 
      gcagcgctc cgccagcct ctaccggcc gcagccgtt 
      cacgccaca gcgctcccg gcccgagtc cctcaactt 
      cgagcccgc gacgcgcgt ctcctcctt ccgccgcga 
      gaccaagac ggcacagac gctcagcat cgtcatggg 
      cggcttcat cgcctgctg gctgccctt cttcatttt 
      ctacctgct gacgccgtt cattcccag caaccaggt 
      caactcgct gatcatgtc ctacttgac gtggctggg 
      ctggttcaa ctcggccat caacccctt catctacgc 
      cttctacag ccccgactt caggatagc cttctggcg 
      actgactgt taagcattt ttcaaagac acgaaggcc 
      gacttcgcc tttcagcac aagtgaggg ccggcgccg 
      gacacgttc gattctagt cacatattt tatagttgt 
      tatttatat tgtactatt gtaatttat tcgcggacg 
      gcgggcgaa tgcttgcga ttattgagc gagcgaatg 
      gtcgcgcgt tgacgggtt ttgttacga ataaggtga 
      agagggatt ggtaagtac acagttaaa ataactcaa 
      aataaaata tgaagcaaa ttacggtaa ttgacattg 
      tttggaaat ggtaattaa tacgcaatt atgactgcg 
      attcataaa tgaattatt tcagttgaa aatccaatt 
      ttccaatac acatattaa tttatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY