Status
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Gene information
General information
TC012121 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5432145..5461238
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012121
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05176

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature complete not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: maybe dead
    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    leg musculature mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs femur shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs tibiotarsus shortened - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012121 RA
      MPSYMYVQT CVWVLIGFF CAGTPVPWL SPSLEALDE 
      LNDAEIYKS VVTSINEWQ KRQELKKIN ARIKREDSS 
      VCYGELGCF EASGPFGYL DMLPSKPEE IDTKFLLYP 
      GHGRRRSGT SPAEVPFSS IKEAFEWAK QGFNKSLPT 
      KVLIHGFGS DCSHIWVYE MRSALMAVE EVNVICVDW 
      EKGAILPNY VKATANTRL VGKQLAMLL RGLVDNNGL 
      SLRTTHLVG FSLGAHVAG FAGAELGNL SRITGLDPA 
      GPLFESQDP RARLDQSDA DFVDVIHSN GENLILGGL 
      GSYQPMGHV DFYPNGGRM QKGCSNLFV GAVSDIIWS 
      SAVEGRSLC NHRRAYKFF TDSVSPRCH FPAFPCESY 
      EDFLSGKCF PCTDERNCG NMGYYADRS KGRGQLYLI 
      TRDEEPFCA HQYHVKIET SNSPVPIVS YGKIQITLI 
      GDSAFNETF MMTRKEDEE MKLGESISR ILVPHPILS 
      EPTSVQILY TAYSGWLSS GLTQWRMDK ITISDSFGK 
      TSSVCKKNL ILDSGVPVT LPLYPGECN LPGHYPKKE 
      AEDRVHDPN PLNLPLNAT KVKNFIPTP VVRIGPEDL 
      EKEYKKAKS GNSIDWKRD EDNRVSDIE EAESSRAFN 
      TKVVKPDKG KESPIKEIV EPILKPHPN KIGRSHDPD 
      RDKPEITEP ILGPTTSRN FGAFKLEPA VKNDFLSRL 
      TSTNKEISY NFYDVEDQN EWIPSIPSP KPKEAKWKR 
      EDGSKAIKT EENPLEVIA TTVQFLPQR LAKMFEQAE 
      KYARETILP LVSTYTPKF ISDIITPRE KPKYVPLTY 
      EEEEGEVVE EEEEESTTK TPRQSAKRN LDNKQFDPP 
      PAFEEASTS KASTTPYTH IQKIRKKTT TPRAETTSA 
      GSRRARDDV HVRKNILEM KLEATQDDL VVDASKKPK 
      AIYVDLPVF ETNPQRKYI PLKDSS
    CDS sequence
    • >TC012121 RA
      atgccgtcc tacatgtac gttcagaca tgtgtgtgg 
      gttctgatc ggatttttt tgcgctgga acaccggtg 
      ccctggctc agcccgtcc ctggaagca ttagacgag 
      ctcaacgac gctgaaatc tacaaatcc gttgttaca 
      tctataaat gaatggcag aaaagacag gagcttaag 
      aaaattaat gccagaatc aaacgcgag gactcctcg 
      gtttgttat ggcgagttg gggtgtttt gaagcctcc 
      ggtcctttc ggttacttg gacatgttg ccaagcaag 
      ccggaggaa atcgacacc aagtttctg ctctaccct 
      ggacacggc cgtcgccgc agcggcaca agcccagct 
      gaagttcct ttttcgtcc attaaagaa gccttcgaa 
      tgggccaag caaggcttt aataaaagc ctacccacg 
      aaggtcctc atccacggc ttcggcagc gactgcagc 
      cacatatgg gtctacgaa atgcgcagc gcccttatg 
      gcggtggag gaggttaac gtgatttgc gtggattgg 
      gagaagggg gcaatcctg cctaattac gttaaagcc 
      accgcaaac actcgcctc gtcggcaag caactggcg 
      atgctcctc cgaggatta gtagataat aacggcttg 
      tcattgcgc accacccat ctggtgggc ttcagcctg 
      ggggcacac gtggcgggc ttcgccggg gccgagttg 
      ggcaacctg agccgcatc acggggctg gaccccgcg 
      gggcccctc ttcgagtcg caggacccg agggcgagg 
      ctggaccag agcgacgcc gacttcgtc gacgttatc 
      cacagcaat ggggagaac ctcatccta ggggggctg 
      gggtcgtat cagcccatg ggccacgtc gacttctac 
      cccaacggc ggacgcatg cagaaaggg tgcagcaat 
      ttgtttgtg ggggctgtc agcgatatc atttggtcg 
      tctgccgtt gagggacga tcgctttgt aaccataga 
      cgagcctat aagttcttt actgactca gtttcgcct 
      cggtgccat ttcccggca ttcccttgc gaatcgtac 
      gaagatttc ctttcgggg aagtgtttt ccgtgcacc 
      gatgagaga aactgcggc aatatgggc tattacgct 
      gacaggtcg aaaggtcga ggacaatta tacctaatc 
      acacgagat gaagagccc ttctgcgcc caccagtat 
      catgtcaaa attgaaacg tccaatagt ccggtcccg 
      atcgtctct tacgggaaa attcaaatc actctaatc 
      ggggactcg gcctttaac gagacgttt atgatgact 
      aggaaagaa gacgaagag atgaagttg ggagaatcc 
      atttcgcgg attttggtc ccgcaccca attttatcg 
      gaacccaca agcgtccag attctgtac actgcgtac 
      agcggctgg ttgtcgtct gggctgacc cagtggagg 
      atggacaag atcaccatc agtgatagt ttcggcaaa 
      acgtcgtct gtgtgcaag aaaaaccta atcctggac 
      tcgggggtg ccggtgacc ctgcccctg taccccggc 
      gagtgcaac ttgcccgga cattacccc aagaaggag 
      gcagaggat cgtgtccac gaccccaac cctctgaac 
      cttcccttg aacgcaaca aaagtgaag aacttcatc 
      ccgactccg gtggtgcgc atcggcccc gaagacttg 
      gaaaaagag tacaaaaaa gcgaaaagc ggaaattcg 
      atcgactgg aaacgcgat gaagacaac agagtttcc 
      gacatcgag gaagctgag tcaagtcgc gcgttcaac 
      accaaagtt gttaagcca gacaagggc aaagaaagc 
      ccgattaag gagatagtc gagccgatt ttgaagccc 
      cacccgaac aaaatcggc cgctcacac gaccctgac 
      cgagacaaa cccgaaatc acggagccc attctaggg 
      cccacaact tcgcgcaac ttcggcgct ttcaaactg 
      gaaccggcc gtgaagaac gactttctg tcgcgtctc 
      acgagcacc aacaaggaa atctcgtac aacttctac 
      gacgtggag gaccagaac gagtggatc ccttcgata 
      ccgtcaccc aagcccaaa gaagccaag tggaaacgt 
      gaggacggc tcgaaggcg atcaaaacg gaggagaac 
      cctctggaa gtgatcgcc acgaccgtc cagttcctg 
      ccacagcgt ctcgccaag atgttcgaa caagcggag 
      aagtacgcc agggagacg atcctcccc ctggtcagc 
      acctacacc cccaagttc atcagcgat ataatcacc 
      ccgagggag aagcccaag tacgtgccc ttgacctac 
      gaggaggag gagggggag gtggtggag gaggaggag 
      gaggagagc acgacgaaa acgccgcgg cagagcgcg 
      aaaagaaac cttgataat aaacagttc gacccgcca 
      ccggccttc gaagaggca agcacgagc aaagcctcg 
      accactccc tacacccat atacaaaaa atcaggaag 
      aaaaccacg actccaagg gcggaaacg acgagtgca 
      gggagtcgg agggcccgt gatgacgtc catgtgcgt 
      aaaaacatc ctcgagatg aaactggag gcgacgcaa 
      gacgaccta gtcgtagac gcctcgaaa aaacccaaa 
      gcgatttac gttgattta ccggttttc gaaactaat 
      ccgcaaagg aagtatatc cccttgaaa gactccagc 
      tag
    mRNA sequence
    • >TC012121 RA
      actggtata acatttagt aggttactg aaataccac 
      tctctacca cagcggttt tcaagcagc tactttaga 
      tcgccagtc taatgcagt gaattgtgg ccgttgcac 
      gtcactttg cctccaaaa cacaaacac acgtgtagt 
      gcttttcgg atactcact cacacactc aaggggaat 
      ccattacca tgagttggc tcatcgagt ttcagtgca 
      gtgctttgt gatgagaat tagtgattg cttaaatct 
      agaccaatg attgtgaaa aacttttgt gatgaacga 
      tgccgtcct acatgtacg ttcagacat gtgtgtggg 
      ttctgatcg gattttttt gcgctggaa caccggtgc 
      cctggctca gcccgtccc tggaagcat tagacgagc 
      tcaacgacg ctgaaatct acaaatccg ttgttacat 
      ctataaatg aatggcaga aaagacagg agcttaaga 
      aaattaatg ccagaatca aacgcgagg actcctcgg 
      tttgttatg gcgagttgg ggtgttttg aagcctccg 
      gtcctttcg gttacttgg acatgttgc caagcaagc 
      cggaggaaa tcgacacca agtttctgc tctaccctg 
      gacacggcc gtcgccgca gcggcacaa gcccagctg 
      aagttcctt tttcgtcca ttaaagaag ccttcgaat 
      gggccaagc aaggcttta ataaaagcc tacccacga 
      aggtcctca tccacggct tcggcagcg actgcagcc 
      acatatggg tctacgaaa tgcgcagcg cccttatgg 
      cggtggagg aggttaacg tgatttgcg tggattggg 
      agaaggggg caatcctgc ctaattacg ttaaagcca 
      ccgcaaaca ctcgcctcg tcggcaagc aactggcga 
      tgctcctcc gaggattag tagataata acggcttgt 
      cattgcgca ccacccatc tggtgggct tcagcctgg 
      gggcacacg tggcgggct tcgccgggg ccgagttgg 
      gcaacctga gccgcatca cggggctgg accccgcgg 
      ggcccctct tcgagtcgc aggacccga gggcgaggc 
      tggaccaga gcgacgccg acttcgtcg acgttatcc 
      acagcaatg gggagaacc tcatcctag gggggctgg 
      ggtcgtatc agcccatgg gccacgtcg acttctacc 
      ccaacggcg gacgcatgc agaaagggt gcagcaatt 
      tgtttgtgg gggctgtca gcgatatca tttggtcgt 
      ctgccgttg agggacgat cgctttgta accatagac 
      gagcctata agttcttta ctgactcag tttcgcctc 
      ggtgccatt tcccggcat tcccttgcg aatcgtacg 
      aagatttcc tttcgggga agtgttttc cgtgcaccg 
      atgagagaa actgcggca atatgggct attacgctg 
      acaggtcga aaggtcgag gacaattat acctaatca 
      cacgagatg aagagccct tctgcgccc accagtatc 
      atgtcaaaa ttgaaacgt ccaatagtc cggtcccga 
      tcgtctctt acgggaaaa ttcaaatca ctctaatcg 
      gggactcgg cctttaacg agacgttta tgatgacta 
      ggaaagaag acgaagaga tgaagttgg gagaatcca 
      tttcgcgga ttttggtcc cgcacccaa ttttatcgg 
      aacccacaa gcgtccaga ttctgtaca ctgcgtaca 
      gcggctggt tgtcgtctg ggctgaccc agtggagga 
      tggacaaga tcaccatca gtgatagtt tcggcaaaa 
      cgtcgtctg tgtgcaaga aaaacctaa tcctggact 
      cgggggtgc cggtgaccc tgcccctgt accccggcg 
      agtgcaact tgcccggac attacccca agaaggagg 
      cagaggatc gtgtccacg accccaacc ctctgaacc 
      ttcccttga acgcaacaa aagtgaaga acttcatcc 
      cgactccgg tggtgcgca tcggccccg aagacttgg 
      aaaaagagt acaaaaaag cgaaaagcg gaaattcga 
      tcgactgga aacgcgatg aagacaaca gagtttccg 
      acatcgagg aagctgagt caagtcgcg cgttcaaca 
      ccaaagttg ttaagccag acaagggca aagaaagcc 
      cgattaagg agatagtcg agccgattt tgaagcccc 
      acccgaaca aaatcggcc gctcacacg accctgacc 
      gagacaaac ccgaaatca cggagccca ttctagggc 
      ccacaactt cgcgcaact tcggcgctt tcaaactgg 
      aaccggccg tgaagaacg actttctgt cgcgtctca 
      cgagcacca acaaggaaa tctcgtaca acttctacg 
      acgtggagg accagaacg agtggatcc cttcgatac 
      cgtcaccca agcccaaag aagccaagt ggaaacgtg 
      aggacggct cgaaggcga tcaaaacgg aggagaacc 
      ctctggaag tgatcgcca cgaccgtcc agttcctgc 
      cacagcgtc tcgccaaga tgttcgaac aagcggaga 
      agtacgcca gggagacga tcctccccc tggtcagca 
      cctacaccc ccaagttca tcagcgata taatcaccc 
      cgagggaga agcccaagt acgtgccct tgacctacg 
      aggaggagg agggggagg tggtggagg aggaggagg 
      aggagagca cgacgaaaa cgccgcggc agagcgcga 
      aaagaaacc ttgataata aacagttcg acccgccac 
      cggccttcg aagaggcaa gcacgagca aagcctcga 
      ccactccct acacccata tacaaaaaa tcaggaaga 
      aaaccacga ctccaaggg cggaaacga cgagtgcag 
      ggagtcgga gggcccgtg atgacgtcc atgtgcgta 
      aaaacatcc tcgagatga aactggagg cgacgcaag 
      acgacctag tcgtagacg cctcgaaaa aacccaaag 
      cgatttacg ttgatttac cggttttcg aaactaatc 
      cgcaaagga agtatatcc ccttgaaag actccagct 
      agatgtaat atatttatt ttatttaat gactgtaaa 
      tatgttttt atacgactg ttgttaatt gtataaatt 
      ttgtaaaat aaaaactat atcagtac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY