Status
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Gene information
General information
TC012119 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5497600..5513119
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012119
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05175

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012119 RA
      MDMTQAYWE ENSANIQRV KYEGCNSND ELGRPKDER 
      GVYQCCETN LNFSTATCS EDDSEYGPY KKNKVTRQD 
      PMSHRIIEK RRRDRMNNC LADLSRLIP TEYLKKGRG 
      RIEKTEIIE MAIKHMKYL QQEHGSPSE HYRMGYQEC 
      MSEAMRFMV EVEGHFPRE GICVRILNH LQKHCESLS 
      RMAQPPAAA NQPVLETRN LQEGGSPQV QVPDYQTPK 
      TEPNNNNGP FTVSLNDTE NYDKHAECN GNGVSYKYK 
      NNIKMRFSQ DLSEGIKRQ KMENGRRAS VNSVENQSS 
      SRSSPPSSE LLTRISEHE PSRTSPTES TSSLNHLKI 
      EPIPRNELR PYSPRTSLD KNFNVPIFV LHAKGSYYV 
      PLTIDYKTL LPFLRNYDI LEVVPGMHN VVLHPVTIN 
      VNFQPNYVN GKYNWN
    CDS sequence
    • >TC012119 RA
      atggacatg acgcaggct tactgggag gaaaactcg 
      gccaacatt caaagggtc aagtacgaa gggtgcaat 
      tcgaatgat gaattaggc cgacccaaa gatgaacga 
      ggtgtttac caatgttgc gagacaaat ttaaatttt 
      tccacggcg acctgtagc gaggacgac tccgaatac 
      ggtccttac aagaaaaac aaagtgaca aggcaagac 
      ccgatgtcg caccgaata atcgaaaag cgacgtcga 
      gaccgcatg aataattgc ctcgccgat ttgtctcgt 
      ctcatcccg acggaatat ttaaaaaaa ggccgaggt 
      cgcatcgag aagaccgag atcatcgag atggccatc 
      aagcacatg aagtacctc cagcaggag cacggcagc 
      ccctcggag cactaccgc atgggctac caggagtgc 
      atgtccgag gccatgcgc ttcatggtg gaggtggag 
      ggccacttc ccccgcgag gggatttgc gtccgcatc 
      ctcaaccac ctgcagaag cactgcgag agcttgtcg 
      cggatggcg cagcccccg gcggccgcc aatcagccc 
      gttttggaa acaaggaac ttgcaagaa ggcggcagt 
      ccacaggtg caagtccct gactaccag acgcccaag 
      actgagccg aacaacaat aatggtccg ttcacagtt 
      agtctgaat gatactgaa aactatgac aaacatgca 
      gaatgtaac ggaaatggg gtctcttac aagtataaa 
      aacaacata aaaatgcgt ttcagtcag gatttgagc 
      gaagggatc aagaggcag aagatggag aacgggcgc 
      agggcgtcc gtcaacagc gtcgaaaac cagagttcc 
      agccgtagt tccccgcct agcagcgaa ttgttgact 
      cgaatctca gagcacgag ccgtcgcgg acttccccc 
      acggagagc acgtcctcg ctcaaccac ctgaagatc 
      gagcccatt ccgcgcaac gagctacgg ccctactcg 
      cctcgaacc tccctggac aagaatttc aacgttccg 
      atattcgtt ttacacgca aaaggatcc tactatgtc 
      cctctgacg atagactac aaaactctg ttgccgttt 
      ttgcgcaac tacgacata ctcgaggtg gttcccggc 
      atgcacaac gttgtcctg catccggtc accatcaac 
      gtcaacttt caaccgaat tatgtcaac gggaagtac 
      aactggaat tga
    mRNA sequence
    • >TC012119 RA
      tcccggagt attccggcg aaaatccgc gcttatcgt 
      cgaaaatcc ggcgagcgc cagtatgtg ttcgccgtc 
      acgtagagt cggaggcag cgcaccgat ttttccttg 
      tcttggatt aaatcctcc gctcacgtg tactctagc 
      gtgttcgtc ggtcggtat cagtggccg aggagtaga 
      tgaaagtga agcgccttt gcgatcgcc gcctgcgac 
      gacacctgt ctcaaatct ccaccgaat ttccggaaa 
      agtgctatg tgctgctga accgtcccc aggaaaatg 
      gacatgacg caggcttac tgggaggaa aactcggcc 
      aacattcaa agggtcaag tacgaaggg tgcaattcg 
      aatgatgaa ttaggccga cccaaagat gaacgaggt 
      gtttaccaa tgttgcgag acaaattta aatttttcc 
      acggcgacc tgtagcgag gacgactcc gaatacggt 
      ccttacaag aaaaacaaa gtgacaagg caagacccg 
      atgtcgcac cgaataatc gaaaagcga cgtcgagac 
      cgcatgaat aattgcctc gccgatttg tctcgtctc 
      atcccgacg gaatattta aaaaaaggc cgaggtcgc 
      atcgagaag accgagatc atcgagatg gccatcaag 
      cacatgaag tacctccag caggagcac ggcagcccc 
      tcggagcac taccgcatg ggctaccag gagtgcatg 
      tccgaggcc atgcgcttc atggtggag gtggagggc 
      cacttcccc cgcgagggg atttgcgtc cgcatcctc 
      aaccacctg cagaagcac tgcgagagc ttgtcgcgg 
      atggcgcag cccccggcg gccgccaat cagcccgtt 
      ttggaaaca aggaacttg caagaaggc ggcagtcca 
      caggtgcaa gtccctgac taccagacg cccaagact 
      gagccgaac aacaataat ggtccgttc acagttagt 
      ctgaatgat actgaaaac tatgacaaa catgcagaa 
      tgtaacgga aatggggtc tcttacaag tataaaaac 
      aacataaaa atgcgtttc agtcaggat ttgagcgaa 
      gggatcaag aggcagaag atggagaac gggcgcagg 
      gcgtccgtc aacagcgtc gaaaaccag agttccagc 
      cgtagttcc ccgcctagc agcgaattg ttgactcga 
      atctcagag cacgagccg tcgcggact tcccccacg 
      gagagcacg tcctcgctc aaccacctg aagatcgag 
      cccattccg cgcaacgag ctacggccc tactcgcct 
      cgaacctcc ctggacaag aatttcaac gttccgata 
      ttcgtttta cacgcaaaa ggatcctac tatgtccct 
      ctgacgata gactacaaa actctgttg ccgtttttg 
      cgcaactac gacatactc gaggtggtt cccggcatg 
      cacaacgtt gtcctgcat ccggtcacc atcaacgtc 
      aactttcaa ccgaattat gtcaacggg aagtacaac 
      tggaattga ggagcgaga atgacgagg gtaaaaagg 
      ctgtgaagg tcaggaaaa tttttaaga ctgatataa 
      ggtttatat agtctaaaa attaaggga gttttgtta 
      ttttttaga tgttacttt tttaagttg gtggttgca 
      gagcgtgca atatgagtt tttagtcct tcagttgat 
      tacagtttc gacagtatt gtgttgaag gaacagtat 
      tcacggttg gtattactg tggaacaag ttgcaacta 
      ctaaaccat tctaccaat a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY