| General information | |||
| TC034305
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 5573990..5612285 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034305 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05172 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
egg chamber not present - Number of animals showing the phenotype: 4
vitellarium potentially size increased - Number of animals showing the phenotype: 1
ovariole potentially size shortened - Number of animals showing the phenotype: 1
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC034305 RA
MKRRWSARV APEESSSEV TSSSALVMS PANSLASTD IGDVDLEFW DLDLNAQNH PRGLTIIQN GYGLPGHTI IASNHHLAK SDTSSASGR EDLSPSSLN GYSADSCDS KKKKGPTPR QQEELCLVC GDRASGYHY NALTCEGCK GFFRRSITK NAVYQCKYG NNCEIDMYM RRKCQECRL KKCLSVGMR PECVVPEVQ CAVKRKEKK AQKEKDKPN STTNGSPDV IKVEPELSD SEKTLCNGS KGISPEQEE LIHRLVYFQ NEYEHPSEE DVKRIINQP MDGEDQCDV RFRHITEIT ILTVQLIVE FAKRLPGFD KLLREDQIA LLKACSSEV MMFRMARRY DVQTDSILF VNNQPYSRD SYNLAGMGE TIEDLLHFC RTMYSMKVD NAEYALLTA IVIFSERPA LIEGWKVEK IQEIYLEAL RAYVDNRRK PKPGTIFAK LLSVLTELR TLGNQNSEM CFSLKLKNK KLPPFLAEI WDVDLKT
- >TC034305 RB
MDIKHEMIY REDDVLLVK SEPQLHSPC ATGSNGLPA GAGSNLLCA SNGLVTTLA SGSSLNGLT NNVPSNGLS SSGPFSSIG GGVSASKRQ RTDDWLSSP SGNVPPLTP SPGPPSHPY TVISNGYSS PMSSGSYDP YSPNGKIGR EDLSPSSLN GYSADSCDS KKKKGPTPR QQEELCLVC GDRASGYHY NALTCEGCK GFFRRSITK NAVYQCKYG NNCEIDMYM RRKCQECRL KKCLSVGMR PECVVPEVQ CAVKRKEKK AQKEKDKPN STTNGSPDV IKVEPELSD SEKTLCNGS KGISPEQEE LIHRLVYFQ NEYEHPSEE DVKRIINQP MDGEDQCDV RFRHITEIT ILTVQLIVE FAKRLPGFD KLLREDQIA LLKACSSEV MMFRMARRY DVQTDSILF VNNQPYSRD SYNLAGMGE TIEDLLHFC RTMYSMKVD NAEYALLTA IVIFSERPA LIEGWKVEK IQEIYLEAL RAYVDNRRK PKPGTIFAK LLSVLTELR TLGNQNSEM CFSLKLKNK KLPPFLAEI WDVDLKT
- >TC034305 RA
atgaaacgc agatggtcg gcgagggtc gctccagag gagagcagc tcggaggtg acgtcgtcg tcggcgctg gtcatgagc cccgccaac tcgctggcg tcgaccgac atcggcgac gtcgacctg gagttctgg gacctggac ctgaacgcg cagaaccac ccccgcggc ctcaccatc atccagaac ggctacggc ctccccggc cacaccatc atcgccagc aaccaccat ctagccaag tccgacacc agctccgca tcaggacgc gaggacctg tccccgagc agcttgaac ggctacagc gctgacagt tgcgactcg aagaagaaa aagggcccg acgccgcgg cagcaggag gagctgtgt ctggtgtgc ggcgacagg gcctccggt taccactac aacgccctc acctgcgag ggctgcaag ggcttcttc cggcgcagc ataacgaag aacgcggtg tatcaatgc aaatacggc aacaattgc gagatagac atgtatatg agacgaaag tgtcaggag tgtcggttg aaaaagtgc ctcagcgtc gggatgagg ccggaatgt gtcgtgccg gaagtacag tgcgccgtc aagcgaaag gagaagaaa gcccaaaaa gagaaagat aagccgaat agcactacg aacggctcg ccggacgtc atcaaggtt gaaccagaa ttgtcagat tcggaaaag acattgtgt aatggaagt aaaggaatt tcgccggag caagaggag ctcatacat cgactggtt tatttccag aatgagtac gaacatccg tctgaggaa gacgttaaa cggatcatt aaccagccg atggatggc gaagatcag tgtgatgtt cggtttagg catatcacg gaaattacc atcttgacg gtgcaactt atcgttgag tttgccaag cggttacca ggctttgac aaactctta agggaagac cagatcgct ctcttgaaa gcatgttcc agcgaagtg atgatgttc aggatggcg cgccgttac gacgtacaa acggattcc atcctcttc gtaaacaac caaccgtat tcaagagac agctacaat ttggctggc atgggggaa accatcgaa gatctcttg catttctgc agaaccatg tattcgatg aaggtggac aacgccgaa tacgcctta ctcacagcc atcgtaata ttctcagag cgtccggcg ctgatcgag ggctggaag gtggagaag atccaggag atctacttg gaggcgctg cgcgcgtac gtggacaac cggaggaag cccaagccg ggcacgata ttcgcgaaa ctcctctca gtactaacc gaattgcgg acgttaggc aaccaaaat tccgagatg tgcttctcg ctaaaactg aaaaacaag aaactgccg ccgttcctg gcggagatc tgggacgtc gacctgaag acatag
- >TC034305 RB
atggacatc aagcacgag atgatttac cgtgaagat gacgtgctg cttgttaaa tcggagccc cagctgcac tcgccctgc gccaccggc agcaacggc ctgcctgcc ggggccggc tcgaacctc ctctgcgcc agcaacggc ctggtgacg actctggcg agcggctcg tcgttgaac ggcctcacc aacaacgtg cccagcaac ggactgtct tctagtgga ccgttcagt tcgatcggt ggtggtgtt agtgctagc aaaaggcag agaaccgac gactggctg agctcgccc agcggcaac gtgccgccc ctgaccccc tcgccgggg cccccctcg cacccgtac accgtcatc agcaacggc tactcctca cccatgtcc tccggcagc tacgacccc tacagcccc aacggaaag atcggacgc gaggacctg tccccgagc agcttgaac ggctacagc gctgacagt tgcgactcg aagaagaaa aagggcccg acgccgcgg cagcaggag gagctgtgt ctggtgtgc ggcgacagg gcctccggt taccactac aacgccctc acctgcgag ggctgcaag ggcttcttc cggcgcagc ataacgaag aacgcggtg tatcaatgc aaatacggc aacaattgc gagatagac atgtatatg agacgaaag tgtcaggag tgtcggttg aaaaagtgc ctcagcgtc gggatgagg ccggaatgt gtcgtgccg gaagtacag tgcgccgtc aagcgaaag gagaagaaa gcccaaaaa gagaaagat aagccgaat agcactacg aacggctcg ccggacgtc atcaaggtt gaaccagaa ttgtcagat tcggaaaag acattgtgt aatggaagt aaaggaatt tcgccggag caagaggag ctcatacat cgactggtt tatttccag aatgagtac gaacatccg tctgaggaa gacgttaaa cggatcatt aaccagccg atggatggc gaagatcag tgtgatgtt cggtttagg catatcacg gaaattacc atcttgacg gtgcaactt atcgttgag tttgccaag cggttacca ggctttgac aaactctta agggaagac cagatcgct ctcttgaaa gcatgttcc agcgaagtg atgatgttc aggatggcg cgccgttac gacgtacaa acggattcc atcctcttc gtaaacaac caaccgtat tcaagagac agctacaat ttggctggc atgggggaa accatcgaa gatctcttg catttctgc agaaccatg tattcgatg aaggtggac aacgccgaa tacgcctta ctcacagcc atcgtaata ttctcagag cgtccggcg ctgatcgag ggctggaag gtggagaag atccaggag atctacttg gaggcgctg cgcgcgtac gtggacaac cggaggaag cccaagccg ggcacgata ttcgcgaaa ctcctctca gtactaacc gaattgcgg acgttaggc aaccaaaat tccgagatg tgcttctcg ctaaaactg aaaaacaag aaactgccg ccgttcctg gcggagatc tgggacgtc gacctgaag acatag
- >TC034305 RA
tcgagtgag ttgttgcgc tgatgttgt ttcgacttt cgtccaaat aaattaatg acacgtgtg gtgatttga acggccggt gcgagatga aacgcagat ggtcggcga gggtcgctc cagaggaga gcagctcgg aggtgacgt cgtcgtcgg cgctggtca tgagccccg ccaactcgc tggcgtcga ccgacatcg gcgacgtcg acctggagt tctgggacc tggacctga acgcgcaga accaccccc gcggcctca ccatcatcc agaacggct acggcctcc ccggccaca ccatcatcg ccagcaacc accatctag ccaagtccg acaccagct ccgcatcag gacgcgagg acctgtccc cgagcagct tgaacggct acagcgctg acagttgcg actcgaaga agaaaaagg gcccgacgc cgcggcagc aggaggagc tgtgtctgg tgtgcggcg acagggcct ccggttacc actacaacg ccctcacct gcgagggct gcaagggct tcttccggc gcagcataa cgaagaacg cggtgtatc aatgcaaat acggcaaca attgcgaga tagacatgt atatgagac gaaagtgtc aggagtgtc ggttgaaaa agtgcctca gcgtcggga tgaggccgg aatgtgtcg tgccggaag tacagtgcg ccgtcaagc gaaaggaga agaaagccc aaaaagaga aagataagc cgaatagca ctacgaacg gctcgccgg acgtcatca aggttgaac cagaattgt cagattcgg aaaagacat tgtgtaatg gaagtaaag gaatttcgc cggagcaag aggagctca tacatcgac tggtttatt tccagaatg agtacgaac atccgtctg aggaagacg ttaaacgga tcattaacc agccgatgg atggcgaag atcagtgtg atgttcggt ttaggcata tcacggaaa ttaccatct tgacggtgc aacttatcg ttgagtttg ccaagcggt taccaggct ttgacaaac tcttaaggg aagaccaga tcgctctct tgaaagcat gttccagcg aagtgatga tgttcagga tggcgcgcc gttacgacg tacaaacgg attccatcc tcttcgtaa acaaccaac cgtattcaa gagacagct acaatttgg ctggcatgg gggaaacca tcgaagatc tcttgcatt tctgcagaa ccatgtatt cgatgaagg tggacaacg ccgaatacg ccttactca cagccatcg taatattct cagagcgtc cggcgctga tcgagggct ggaaggtgg agaagatcc aggagatct acttggagg cgctgcgcg cgtacgtgg acaaccgga ggaagccca agccgggca cgatattcg cgaaactcc tctcagtac taaccgaat tgcggacgt taggcaacc aaaattccg agatgtgct tctcgctaa aactgaaaa acaagaaac tgccgccgt tcctggcgg agatctggg acgtcgacc tgaagacat agtagaggt gacgtaccg acaggtgga tgtttgctg ctgtttgga gggcggatt tcgaatacg acttaataa tggcagtgg atcggctca ccaccgaaa gttggctgt gaaataatg taaattttt tttattaat taattatta aacgaatgt tggacggca tctagagaa aatgttatt actgagcat agatttatt tagataatt acatagaga atattactc gatgaatca aactattat gtagatttt ataaatgcc tgtataaac tctgcgact tttgtaaaa cactttatc ttggcaatt tgcttactt aattggtaa gactggacc ggactgact taacagagg tgccttcac ttgaatacg gatcatacg actgagggc aggtgcaaa ttatgacaa ttacaatgc cagtagagt taggccgac tggaatgat ctgaaatta tgaaaaagt gcaatttaa tttatttca aattaccaa acgaatcgt ttagacgtc taaagcacc aatctacgg caaaggagg cagaattac ttcaacaat aatcgtaac aaagaagca gccgtccgc tataatccc aaatagtga aacgtgaag tgattcgga tctgacagt gaacgagga ctcgtgcca ttttatccg ggcatcctg tcatacata cgtataacc acactatac aacagacta tcctgcctt tacaaacta ttgatgttt aactcttcg atgtgtagt tgtagccta ctcattttt atactgtac tgtatagtt tctgcattc gtattctgc tgctagatg cgctcgtat tataacgac atatttttc atcaactgt agatagaaa catattttc ataacattc agagacaga tgtattttt gttggacac agagttatt gtttatttg cgcgcgcca tctattgcc ttgccacca agcacacgg tttagttaa caccgggag tcaaccatc ctgactgga cccaccaac ctatcttat catttcagc attgcgttc caaagtgca aggtgctat gaacggccc tgaacccgc atctattaa tacgcaaca catcacgat caaaaaaag attgtttaa ctccttttt attgatttt ggatgctaa acagattgt ttcgtttat tacactttt attttatgt aacaaacac acaacgtca cggaatatc acgctgttg aaaaaatta ttactttta tcaatttgg aattatgct aatacatag cgtacaaat actcaaaca ttatcgcat aatcacgaa cctgtcacg tttcttttg tgtttcacc aagctgtta tttgttgtg ggaacgagc aaatcggtt aattaaatc ctgacataa aggtcttgt cagttttcg gtttttcaa ccgatttca aaagtgcaa ttgaaccgc aggaggttg tttaataaa tttcttatt cggcgaatg tgagaatta tctcatgac attgttttc acaccgatt tattatttc ggtagcata cagtgtgat gcttttaag gtttatttc gttatttta ttgtgtatg gattaattg atccactga gttattgta aaattttat gtatatttg atcatattt tttaagtta tgttttaaa ccccgtcaa agacacaaa ttgtgatta cagcgatta aaaatccaa acaattgcg aagttttga ttgttttat gatgttttt ctgtgataa aatacgaaa aaaatattt gaatagtcg ggtgggatt atagaaagc tcacttaaa atttaatgc gctgattgg ttcaatttg cggattaaa ctaatgagg cttaggccc gttatgtca atgttgagt taaatataa actttaaat taag
- >TC034305 RB
tgactctga ctgtacatt ttaatttaa cacagcgtc aatagcttt ccaagactt cccacgggg gcagcgaaa gcgcaaccc ctttaacaa gcaatctat cgtcttgtt tattggatc gcagcgcca atttggttt tttgttgca gttgttgtt agtatgtct cgcttggat ccgcccccc cggtaatcc cagttaatg acagccata tgtagcggt aatttgggc agcatggac atcaagcac gagatgatt taccgtgaa gatgacgtg ctgcttgtt aaatcggag ccccagctg cactcgccc tgcgccacc ggcagcaac ggcctgcct gccggggcc ggctcgaac ctcctctgc gccagcaac ggcctggtg acgactctg gcgagcggc tcgtcgttg aacggcctc accaacaac gtgcccagc aacggactg tcttctagt ggaccgttc agttcgatc ggtggtggt gttagtgct agcaaaagg cagagaacc gacgactgg ctgagctcg cccagcggc aacgtgccg cccctgacc ccctcgccg gggcccccc tcgcacccg tacaccgtc atcagcaac ggctactcc tcacccatg tcctccggc agctacgac ccctacagc cccaacgga aagatcgga cgcgaggac ctgtccccg agcagcttg aacggctac agcgctgac agttgcgac tcgaagaag aaaaagggc ccgacgccg cggcagcag gaggagctg tgtctggtg tgcggcgac agggcctcc ggttaccac tacaacgcc ctcacctgc gagggctgc aagggcttc ttccggcgc agcataacg aagaacgcg gtgtatcaa tgcaaatac ggcaacaat tgcgagata gacatgtat atgagacga aagtgtcag gagtgtcgg ttgaaaaag tgcctcagc gtcgggatg aggccggaa tgtgtcgtg ccggaagta cagtgcgcc gtcaagcga aaggagaag aaagcccaa aaagagaaa gataagccg aatagcact acgaacggc tcgccggac gtcatcaag gttgaacca gaattgtca gattcggaa aagacattg tgtaatgga agtaaagga atttcgccg gagcaagag gagctcata catcgactg gtttatttc cagaatgag tacgaacat ccgtctgag gaagacgtt aaacggatc attaaccag ccgatggat ggcgaagat cagtgtgat gttcggttt aggcatatc acggaaatt accatcttg acggtgcaa cttatcgtt gagtttgcc aagcggtta ccaggcttt gacaaactc ttaagggaa gaccagatc gctctcttg aaagcatgt tccagcgaa gtgatgatg ttcaggatg gcgcgccgt tacgacgta caaacggat tccatcctc ttcgtaaac aaccaaccg tattcaaga gacagctac aatttggct ggcatgggg gaaaccatc gaagatctc ttgcatttc tgcagaacc atgtattcg atgaaggtg gacaacgcc gaatacgcc ttactcaca gccatcgta atattctca gagcgtccg gcgctgatc gagggctgg aaggtggag aagatccag gagatctac ttggaggcg ctgcgcgcg tacgtggac aaccggagg aagcccaag ccgggcacg atattcgcg aaactcctc tcagtacta accgaattg cggacgtta ggcaaccaa aattccgag atgtgcttc tcgctaaaa ctgaaaaac aagaaactg ccgccgttc ctggcggag atctgggac gtcgacctg aagacatag tagaggtga cgtaccgac aggtggatg tttgctgct gtttggagg gcggatttc gaatacgac ttaataatg gcagtggat cggctcacc accgaaagt tggctgtga aataatgta aattttttt tattaatta attattaaa cgaatgttg gacggcatc tagagaaaa tgttattac tgagcatag atttattta gataattac atagagaat attactcga tgaatcaaa ctattatgt agattttat aaatgcctg tataaactc tgcgacttt tgtaaaaca ctttatctt ggcaatttg cttacttaa ttggtaaga ctggaccgg actgactta acagaggtg ccttcactt gaatacgga tcatacgac tgagggcag gtgcaaatt atgacaatt acaatgcca gtagagtta ggccgactg gaatgatct gaaattatg aaaaagtgc aatttaatt tatttcaaa ttaccaaac gaatcgttt agacgtcta aagcaccaa tctacggca aaggaggca gaattactt caacaataa tcgtaacaa agaagcagc cgtccgcta taatcccaa atagtgaaa cgtgaagtg attcggatc tgacagtga acgaggact cgtgccatt ttatccggg catcctgtc atacatacg tataaccac actatacaa cagactatc ctgccttta caaactatt gatgtttaa ctcttcgat gtgtagttg tagcctact catttttat actgtactg tatagtttc tgcattcgt attctgctg ctagatgcg ctcgtatta taacgacat atttttcat caactgtag atagaaaca tattttcat aacattcag agacagatg tatttttgt tggacacag agttattgt ttatttgcg cgcgccatc tattgcctt gccaccaag cacacggtt tagttaaca ccgggagtc aaccatcct gactggacc caccaacct atcttatca tttcagcat tgcgttcca aagtgcaag gtgctatga acggccctg aacccgcat ctattaata cgcaacaca tcacgatca aaaaaagat tgtttaact cctttttat tgattttgg atgctaaac agattgttt cgtttatta cacttttat tttatgtaa caaacacac aacgtcacg gaatatcac gctgttgaa aaaattatt acttttatc aatttggaa ttatgctaa tacatagcg tacaaatac tcaaacatt atcgcataa tcacgaacc tgtcacgtt tcttttgtg tttcaccaa gctgttatt tgttgtggg aacgagcaa atcggttaa ttaaatcct gacataaag gtcttgtca gttttcggt ttttcaacc gatttcaaa agtgcaatt gaaccgcag gaggttgtt taataaatt tcttattcg gcgaatgtg agaattatc tcatgacat tgttttcac accgattta ttatttcgg tagcataca gtgtgatgc ttttaaggt ttatttcgt tattttatt gtgtatgga ttaattgat ccactgagt tattgtaaa attttatgt atatttgat catattttt taagttatg ttttaaacc ccgtcaaag acacaaatt gtgattaca gcgattaaa aatccaaac aattgcgaa gttttgatt gttttatga tgtttttct gtgataaaa tacgaaaaa aatatttga atagtcggg tgggattat agaaagctc acttaaaat ttaatgcgc tgattggtt caatttgcg gattaaact aatgaggct taggcccgt tatgtcaat gttgagtta aatataaac tttaaatta ag
