Status
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Gene information
General information
TC034305
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC012112,TC012113
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5573990..5612285
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034305
    iBeetle screen iB_05172

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    egg chamber not present - Number of animals showing the phenotype: 4

    vitellarium potentially size increased - Number of animals showing the phenotype: 1

    ovariole potentially size shortened - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034305 RA
      MKRRWSARV APEESSSEV TSSSALVMS PANSLASTD 
      IGDVDLEFW DLDLNAQNH PRGLTIIQN GYGLPGHTI 
      IASNHHLAK SDTSSASGR EDLSPSSLN GYSADSCDS 
      KKKKGPTPR QQEELCLVC GDRASGYHY NALTCEGCK 
      GFFRRSITK NAVYQCKYG NNCEIDMYM RRKCQECRL 
      KKCLSVGMR PECVVPEVQ CAVKRKEKK AQKEKDKPN 
      STTNGSPDV IKVEPELSD SEKTLCNGS KGISPEQEE 
      LIHRLVYFQ NEYEHPSEE DVKRIINQP MDGEDQCDV 
      RFRHITEIT ILTVQLIVE FAKRLPGFD KLLREDQIA 
      LLKACSSEV MMFRMARRY DVQTDSILF VNNQPYSRD 
      SYNLAGMGE TIEDLLHFC RTMYSMKVD NAEYALLTA 
      IVIFSERPA LIEGWKVEK IQEIYLEAL RAYVDNRRK 
      PKPGTIFAK LLSVLTELR TLGNQNSEM CFSLKLKNK 
      KLPPFLAEI WDVDLKT
    • >TC034305 RB
      MDIKHEMIY REDDVLLVK SEPQLHSPC ATGSNGLPA 
      GAGSNLLCA SNGLVTTLA SGSSLNGLT NNVPSNGLS 
      SSGPFSSIG GGVSASKRQ RTDDWLSSP SGNVPPLTP 
      SPGPPSHPY TVISNGYSS PMSSGSYDP YSPNGKIGR 
      EDLSPSSLN GYSADSCDS KKKKGPTPR QQEELCLVC 
      GDRASGYHY NALTCEGCK GFFRRSITK NAVYQCKYG 
      NNCEIDMYM RRKCQECRL KKCLSVGMR PECVVPEVQ 
      CAVKRKEKK AQKEKDKPN STTNGSPDV IKVEPELSD 
      SEKTLCNGS KGISPEQEE LIHRLVYFQ NEYEHPSEE 
      DVKRIINQP MDGEDQCDV RFRHITEIT ILTVQLIVE 
      FAKRLPGFD KLLREDQIA LLKACSSEV MMFRMARRY 
      DVQTDSILF VNNQPYSRD SYNLAGMGE TIEDLLHFC 
      RTMYSMKVD NAEYALLTA IVIFSERPA LIEGWKVEK 
      IQEIYLEAL RAYVDNRRK PKPGTIFAK LLSVLTELR 
      TLGNQNSEM CFSLKLKNK KLPPFLAEI WDVDLKT
    CDS sequence
    • >TC034305 RA
      atgaaacgc agatggtcg gcgagggtc gctccagag 
      gagagcagc tcggaggtg acgtcgtcg tcggcgctg 
      gtcatgagc cccgccaac tcgctggcg tcgaccgac 
      atcggcgac gtcgacctg gagttctgg gacctggac 
      ctgaacgcg cagaaccac ccccgcggc ctcaccatc 
      atccagaac ggctacggc ctccccggc cacaccatc 
      atcgccagc aaccaccat ctagccaag tccgacacc 
      agctccgca tcaggacgc gaggacctg tccccgagc 
      agcttgaac ggctacagc gctgacagt tgcgactcg 
      aagaagaaa aagggcccg acgccgcgg cagcaggag 
      gagctgtgt ctggtgtgc ggcgacagg gcctccggt 
      taccactac aacgccctc acctgcgag ggctgcaag 
      ggcttcttc cggcgcagc ataacgaag aacgcggtg 
      tatcaatgc aaatacggc aacaattgc gagatagac 
      atgtatatg agacgaaag tgtcaggag tgtcggttg 
      aaaaagtgc ctcagcgtc gggatgagg ccggaatgt 
      gtcgtgccg gaagtacag tgcgccgtc aagcgaaag 
      gagaagaaa gcccaaaaa gagaaagat aagccgaat 
      agcactacg aacggctcg ccggacgtc atcaaggtt 
      gaaccagaa ttgtcagat tcggaaaag acattgtgt 
      aatggaagt aaaggaatt tcgccggag caagaggag 
      ctcatacat cgactggtt tatttccag aatgagtac 
      gaacatccg tctgaggaa gacgttaaa cggatcatt 
      aaccagccg atggatggc gaagatcag tgtgatgtt 
      cggtttagg catatcacg gaaattacc atcttgacg 
      gtgcaactt atcgttgag tttgccaag cggttacca 
      ggctttgac aaactctta agggaagac cagatcgct 
      ctcttgaaa gcatgttcc agcgaagtg atgatgttc 
      aggatggcg cgccgttac gacgtacaa acggattcc 
      atcctcttc gtaaacaac caaccgtat tcaagagac 
      agctacaat ttggctggc atgggggaa accatcgaa 
      gatctcttg catttctgc agaaccatg tattcgatg 
      aaggtggac aacgccgaa tacgcctta ctcacagcc 
      atcgtaata ttctcagag cgtccggcg ctgatcgag 
      ggctggaag gtggagaag atccaggag atctacttg 
      gaggcgctg cgcgcgtac gtggacaac cggaggaag 
      cccaagccg ggcacgata ttcgcgaaa ctcctctca 
      gtactaacc gaattgcgg acgttaggc aaccaaaat 
      tccgagatg tgcttctcg ctaaaactg aaaaacaag 
      aaactgccg ccgttcctg gcggagatc tgggacgtc 
      gacctgaag acatag
    • >TC034305 RB
      atggacatc aagcacgag atgatttac cgtgaagat 
      gacgtgctg cttgttaaa tcggagccc cagctgcac 
      tcgccctgc gccaccggc agcaacggc ctgcctgcc 
      ggggccggc tcgaacctc ctctgcgcc agcaacggc 
      ctggtgacg actctggcg agcggctcg tcgttgaac 
      ggcctcacc aacaacgtg cccagcaac ggactgtct 
      tctagtgga ccgttcagt tcgatcggt ggtggtgtt 
      agtgctagc aaaaggcag agaaccgac gactggctg 
      agctcgccc agcggcaac gtgccgccc ctgaccccc 
      tcgccgggg cccccctcg cacccgtac accgtcatc 
      agcaacggc tactcctca cccatgtcc tccggcagc 
      tacgacccc tacagcccc aacggaaag atcggacgc 
      gaggacctg tccccgagc agcttgaac ggctacagc 
      gctgacagt tgcgactcg aagaagaaa aagggcccg 
      acgccgcgg cagcaggag gagctgtgt ctggtgtgc 
      ggcgacagg gcctccggt taccactac aacgccctc 
      acctgcgag ggctgcaag ggcttcttc cggcgcagc 
      ataacgaag aacgcggtg tatcaatgc aaatacggc 
      aacaattgc gagatagac atgtatatg agacgaaag 
      tgtcaggag tgtcggttg aaaaagtgc ctcagcgtc 
      gggatgagg ccggaatgt gtcgtgccg gaagtacag 
      tgcgccgtc aagcgaaag gagaagaaa gcccaaaaa 
      gagaaagat aagccgaat agcactacg aacggctcg 
      ccggacgtc atcaaggtt gaaccagaa ttgtcagat 
      tcggaaaag acattgtgt aatggaagt aaaggaatt 
      tcgccggag caagaggag ctcatacat cgactggtt 
      tatttccag aatgagtac gaacatccg tctgaggaa 
      gacgttaaa cggatcatt aaccagccg atggatggc 
      gaagatcag tgtgatgtt cggtttagg catatcacg 
      gaaattacc atcttgacg gtgcaactt atcgttgag 
      tttgccaag cggttacca ggctttgac aaactctta 
      agggaagac cagatcgct ctcttgaaa gcatgttcc 
      agcgaagtg atgatgttc aggatggcg cgccgttac 
      gacgtacaa acggattcc atcctcttc gtaaacaac 
      caaccgtat tcaagagac agctacaat ttggctggc 
      atgggggaa accatcgaa gatctcttg catttctgc 
      agaaccatg tattcgatg aaggtggac aacgccgaa 
      tacgcctta ctcacagcc atcgtaata ttctcagag 
      cgtccggcg ctgatcgag ggctggaag gtggagaag 
      atccaggag atctacttg gaggcgctg cgcgcgtac 
      gtggacaac cggaggaag cccaagccg ggcacgata 
      ttcgcgaaa ctcctctca gtactaacc gaattgcgg 
      acgttaggc aaccaaaat tccgagatg tgcttctcg 
      ctaaaactg aaaaacaag aaactgccg ccgttcctg 
      gcggagatc tgggacgtc gacctgaag acatag
    mRNA sequence
    • >TC034305 RA
      tcgagtgag ttgttgcgc tgatgttgt ttcgacttt 
      cgtccaaat aaattaatg acacgtgtg gtgatttga 
      acggccggt gcgagatga aacgcagat ggtcggcga 
      gggtcgctc cagaggaga gcagctcgg aggtgacgt 
      cgtcgtcgg cgctggtca tgagccccg ccaactcgc 
      tggcgtcga ccgacatcg gcgacgtcg acctggagt 
      tctgggacc tggacctga acgcgcaga accaccccc 
      gcggcctca ccatcatcc agaacggct acggcctcc 
      ccggccaca ccatcatcg ccagcaacc accatctag 
      ccaagtccg acaccagct ccgcatcag gacgcgagg 
      acctgtccc cgagcagct tgaacggct acagcgctg 
      acagttgcg actcgaaga agaaaaagg gcccgacgc 
      cgcggcagc aggaggagc tgtgtctgg tgtgcggcg 
      acagggcct ccggttacc actacaacg ccctcacct 
      gcgagggct gcaagggct tcttccggc gcagcataa 
      cgaagaacg cggtgtatc aatgcaaat acggcaaca 
      attgcgaga tagacatgt atatgagac gaaagtgtc 
      aggagtgtc ggttgaaaa agtgcctca gcgtcggga 
      tgaggccgg aatgtgtcg tgccggaag tacagtgcg 
      ccgtcaagc gaaaggaga agaaagccc aaaaagaga 
      aagataagc cgaatagca ctacgaacg gctcgccgg 
      acgtcatca aggttgaac cagaattgt cagattcgg 
      aaaagacat tgtgtaatg gaagtaaag gaatttcgc 
      cggagcaag aggagctca tacatcgac tggtttatt 
      tccagaatg agtacgaac atccgtctg aggaagacg 
      ttaaacgga tcattaacc agccgatgg atggcgaag 
      atcagtgtg atgttcggt ttaggcata tcacggaaa 
      ttaccatct tgacggtgc aacttatcg ttgagtttg 
      ccaagcggt taccaggct ttgacaaac tcttaaggg 
      aagaccaga tcgctctct tgaaagcat gttccagcg 
      aagtgatga tgttcagga tggcgcgcc gttacgacg 
      tacaaacgg attccatcc tcttcgtaa acaaccaac 
      cgtattcaa gagacagct acaatttgg ctggcatgg 
      gggaaacca tcgaagatc tcttgcatt tctgcagaa 
      ccatgtatt cgatgaagg tggacaacg ccgaatacg 
      ccttactca cagccatcg taatattct cagagcgtc 
      cggcgctga tcgagggct ggaaggtgg agaagatcc 
      aggagatct acttggagg cgctgcgcg cgtacgtgg 
      acaaccgga ggaagccca agccgggca cgatattcg 
      cgaaactcc tctcagtac taaccgaat tgcggacgt 
      taggcaacc aaaattccg agatgtgct tctcgctaa 
      aactgaaaa acaagaaac tgccgccgt tcctggcgg 
      agatctggg acgtcgacc tgaagacat agtagaggt 
      gacgtaccg acaggtgga tgtttgctg ctgtttgga 
      gggcggatt tcgaatacg acttaataa tggcagtgg 
      atcggctca ccaccgaaa gttggctgt gaaataatg 
      taaattttt tttattaat taattatta aacgaatgt 
      tggacggca tctagagaa aatgttatt actgagcat 
      agatttatt tagataatt acatagaga atattactc 
      gatgaatca aactattat gtagatttt ataaatgcc 
      tgtataaac tctgcgact tttgtaaaa cactttatc 
      ttggcaatt tgcttactt aattggtaa gactggacc 
      ggactgact taacagagg tgccttcac ttgaatacg 
      gatcatacg actgagggc aggtgcaaa ttatgacaa 
      ttacaatgc cagtagagt taggccgac tggaatgat 
      ctgaaatta tgaaaaagt gcaatttaa tttatttca 
      aattaccaa acgaatcgt ttagacgtc taaagcacc 
      aatctacgg caaaggagg cagaattac ttcaacaat 
      aatcgtaac aaagaagca gccgtccgc tataatccc 
      aaatagtga aacgtgaag tgattcgga tctgacagt 
      gaacgagga ctcgtgcca ttttatccg ggcatcctg 
      tcatacata cgtataacc acactatac aacagacta 
      tcctgcctt tacaaacta ttgatgttt aactcttcg 
      atgtgtagt tgtagccta ctcattttt atactgtac 
      tgtatagtt tctgcattc gtattctgc tgctagatg 
      cgctcgtat tataacgac atatttttc atcaactgt 
      agatagaaa catattttc ataacattc agagacaga 
      tgtattttt gttggacac agagttatt gtttatttg 
      cgcgcgcca tctattgcc ttgccacca agcacacgg 
      tttagttaa caccgggag tcaaccatc ctgactgga 
      cccaccaac ctatcttat catttcagc attgcgttc 
      caaagtgca aggtgctat gaacggccc tgaacccgc 
      atctattaa tacgcaaca catcacgat caaaaaaag 
      attgtttaa ctccttttt attgatttt ggatgctaa 
      acagattgt ttcgtttat tacactttt attttatgt 
      aacaaacac acaacgtca cggaatatc acgctgttg 
      aaaaaatta ttactttta tcaatttgg aattatgct 
      aatacatag cgtacaaat actcaaaca ttatcgcat 
      aatcacgaa cctgtcacg tttcttttg tgtttcacc 
      aagctgtta tttgttgtg ggaacgagc aaatcggtt 
      aattaaatc ctgacataa aggtcttgt cagttttcg 
      gtttttcaa ccgatttca aaagtgcaa ttgaaccgc 
      aggaggttg tttaataaa tttcttatt cggcgaatg 
      tgagaatta tctcatgac attgttttc acaccgatt 
      tattatttc ggtagcata cagtgtgat gcttttaag 
      gtttatttc gttatttta ttgtgtatg gattaattg 
      atccactga gttattgta aaattttat gtatatttg 
      atcatattt tttaagtta tgttttaaa ccccgtcaa 
      agacacaaa ttgtgatta cagcgatta aaaatccaa 
      acaattgcg aagttttga ttgttttat gatgttttt 
      ctgtgataa aatacgaaa aaaatattt gaatagtcg 
      ggtgggatt atagaaagc tcacttaaa atttaatgc 
      gctgattgg ttcaatttg cggattaaa ctaatgagg 
      cttaggccc gttatgtca atgttgagt taaatataa 
      actttaaat taag
    • >TC034305 RB
      tgactctga ctgtacatt ttaatttaa cacagcgtc 
      aatagcttt ccaagactt cccacgggg gcagcgaaa 
      gcgcaaccc ctttaacaa gcaatctat cgtcttgtt 
      tattggatc gcagcgcca atttggttt tttgttgca 
      gttgttgtt agtatgtct cgcttggat ccgcccccc 
      cggtaatcc cagttaatg acagccata tgtagcggt 
      aatttgggc agcatggac atcaagcac gagatgatt 
      taccgtgaa gatgacgtg ctgcttgtt aaatcggag 
      ccccagctg cactcgccc tgcgccacc ggcagcaac 
      ggcctgcct gccggggcc ggctcgaac ctcctctgc 
      gccagcaac ggcctggtg acgactctg gcgagcggc 
      tcgtcgttg aacggcctc accaacaac gtgcccagc 
      aacggactg tcttctagt ggaccgttc agttcgatc 
      ggtggtggt gttagtgct agcaaaagg cagagaacc 
      gacgactgg ctgagctcg cccagcggc aacgtgccg 
      cccctgacc ccctcgccg gggcccccc tcgcacccg 
      tacaccgtc atcagcaac ggctactcc tcacccatg 
      tcctccggc agctacgac ccctacagc cccaacgga 
      aagatcgga cgcgaggac ctgtccccg agcagcttg 
      aacggctac agcgctgac agttgcgac tcgaagaag 
      aaaaagggc ccgacgccg cggcagcag gaggagctg 
      tgtctggtg tgcggcgac agggcctcc ggttaccac 
      tacaacgcc ctcacctgc gagggctgc aagggcttc 
      ttccggcgc agcataacg aagaacgcg gtgtatcaa 
      tgcaaatac ggcaacaat tgcgagata gacatgtat 
      atgagacga aagtgtcag gagtgtcgg ttgaaaaag 
      tgcctcagc gtcgggatg aggccggaa tgtgtcgtg 
      ccggaagta cagtgcgcc gtcaagcga aaggagaag 
      aaagcccaa aaagagaaa gataagccg aatagcact 
      acgaacggc tcgccggac gtcatcaag gttgaacca 
      gaattgtca gattcggaa aagacattg tgtaatgga 
      agtaaagga atttcgccg gagcaagag gagctcata 
      catcgactg gtttatttc cagaatgag tacgaacat 
      ccgtctgag gaagacgtt aaacggatc attaaccag 
      ccgatggat ggcgaagat cagtgtgat gttcggttt 
      aggcatatc acggaaatt accatcttg acggtgcaa 
      cttatcgtt gagtttgcc aagcggtta ccaggcttt 
      gacaaactc ttaagggaa gaccagatc gctctcttg 
      aaagcatgt tccagcgaa gtgatgatg ttcaggatg 
      gcgcgccgt tacgacgta caaacggat tccatcctc 
      ttcgtaaac aaccaaccg tattcaaga gacagctac 
      aatttggct ggcatgggg gaaaccatc gaagatctc 
      ttgcatttc tgcagaacc atgtattcg atgaaggtg 
      gacaacgcc gaatacgcc ttactcaca gccatcgta 
      atattctca gagcgtccg gcgctgatc gagggctgg 
      aaggtggag aagatccag gagatctac ttggaggcg 
      ctgcgcgcg tacgtggac aaccggagg aagcccaag 
      ccgggcacg atattcgcg aaactcctc tcagtacta 
      accgaattg cggacgtta ggcaaccaa aattccgag 
      atgtgcttc tcgctaaaa ctgaaaaac aagaaactg 
      ccgccgttc ctggcggag atctgggac gtcgacctg 
      aagacatag tagaggtga cgtaccgac aggtggatg 
      tttgctgct gtttggagg gcggatttc gaatacgac 
      ttaataatg gcagtggat cggctcacc accgaaagt 
      tggctgtga aataatgta aattttttt tattaatta 
      attattaaa cgaatgttg gacggcatc tagagaaaa 
      tgttattac tgagcatag atttattta gataattac 
      atagagaat attactcga tgaatcaaa ctattatgt 
      agattttat aaatgcctg tataaactc tgcgacttt 
      tgtaaaaca ctttatctt ggcaatttg cttacttaa 
      ttggtaaga ctggaccgg actgactta acagaggtg 
      ccttcactt gaatacgga tcatacgac tgagggcag 
      gtgcaaatt atgacaatt acaatgcca gtagagtta 
      ggccgactg gaatgatct gaaattatg aaaaagtgc 
      aatttaatt tatttcaaa ttaccaaac gaatcgttt 
      agacgtcta aagcaccaa tctacggca aaggaggca 
      gaattactt caacaataa tcgtaacaa agaagcagc 
      cgtccgcta taatcccaa atagtgaaa cgtgaagtg 
      attcggatc tgacagtga acgaggact cgtgccatt 
      ttatccggg catcctgtc atacatacg tataaccac 
      actatacaa cagactatc ctgccttta caaactatt 
      gatgtttaa ctcttcgat gtgtagttg tagcctact 
      catttttat actgtactg tatagtttc tgcattcgt 
      attctgctg ctagatgcg ctcgtatta taacgacat 
      atttttcat caactgtag atagaaaca tattttcat 
      aacattcag agacagatg tatttttgt tggacacag 
      agttattgt ttatttgcg cgcgccatc tattgcctt 
      gccaccaag cacacggtt tagttaaca ccgggagtc 
      aaccatcct gactggacc caccaacct atcttatca 
      tttcagcat tgcgttcca aagtgcaag gtgctatga 
      acggccctg aacccgcat ctattaata cgcaacaca 
      tcacgatca aaaaaagat tgtttaact cctttttat 
      tgattttgg atgctaaac agattgttt cgtttatta 
      cacttttat tttatgtaa caaacacac aacgtcacg 
      gaatatcac gctgttgaa aaaattatt acttttatc 
      aatttggaa ttatgctaa tacatagcg tacaaatac 
      tcaaacatt atcgcataa tcacgaacc tgtcacgtt 
      tcttttgtg tttcaccaa gctgttatt tgttgtggg 
      aacgagcaa atcggttaa ttaaatcct gacataaag 
      gtcttgtca gttttcggt ttttcaacc gatttcaaa 
      agtgcaatt gaaccgcag gaggttgtt taataaatt 
      tcttattcg gcgaatgtg agaattatc tcatgacat 
      tgttttcac accgattta ttatttcgg tagcataca 
      gtgtgatgc ttttaaggt ttatttcgt tattttatt 
      gtgtatgga ttaattgat ccactgagt tattgtaaa 
      attttatgt atatttgat catattttt taagttatg 
      ttttaaacc ccgtcaaag acacaaatt gtgattaca 
      gcgattaaa aatccaaac aattgcgaa gttttgatt 
      gttttatga tgtttttct gtgataaaa tacgaaaaa 
      aatatttga atagtcggg tgggattat agaaagctc 
      acttaaaat ttaatgcgc tgattggtt caatttgcg 
      gattaaact aatgaggct taggcccgt tatgtcaat 
      gttgagtta aatataaac tttaaatta ag
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