| General information | |||
| TC034867 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 5723603..5748300 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC034867 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05171 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Days post injection
Fertility
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (19 dpi)
Days post injection
adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
Days post injection
Pupal morphology
- >TC034867 RA
MMFTGGSHY KKLTSANSG SGRRGPRKH AEGKSRPSR QTPKAIMVH RNYEGSLGS NSTTSLLKS NSSIESDED DFQRTRGRE ITRRRGAVK HQRVHEVKG HKFLAKFFR QPTFCAFCK DFLWGFCKQ GYQCQACQT AVHKRCHDK LLGKCPESG RESESTIYL RERFKIDLP HRFKIHTFM SPTFCDHCG SLLYGIYKQ GLKCEAGDF YLLASARGM DASNSSYTG SYIPGPYSN HSSLLASNF TRPPPSLLK PRNFRGYKP LLATITENQ SPLRRYNSP KLRGHTSPK VIVAKPIKI NTADIDVSV NKYRKYERP KPVQDKVER KSPSPSDTL DSPRSSEKE AKPNKTIRR DRPTVRLHT VHCDMIAPD NGANTRSWR DNFEPEELK TEEKKPKKT PGELLKEKF LIRSREDIF KAPEKKVSR RASKKTNVQ KTPSFHDIC QAITTDQID EELNPGQPI EIQRRQSRQ FASENLLKD LRRNSGDLT NEVELLNRR NAELSDTRG SVKRRKKLT KKKSNEKVV LTEPDGNKS QLQRRPTQK KIRRTSTSS SVGSDIVDV SPETEKAYQ EAIRDISQV EIENIKIKP KPIITATVD VEEKPALKV VVDEIEVVE SPKPKKGNK FKFNVIVEE IDTKRTSMK KPQTKVEEK VHVPKAKEP LVSNILRKN SNAHLGNIL EEAVNKSEA EVKKSDSAE EMKKVVVVK TISKKEDKE PVQIRKTDS DDKVVVSKE VAQERANQD QTSGQQLKQ SSSVPDMEM KKNEVGKPK EDEKSTSAK KVEAPLKKE LSLDKVKKE DEKSTAAKK AESQLKKDS SIEKITSGN VVKLKEHEE STTVKKIEP ELKKNLSLE KINDKVERP KEDEKSTAM KKVEPQLKK NLSLEKINI DKVEKPKED KPTVVKKVE PQLKKNSSL EKINNDKVE IPKEDEKSM AVKKVEPQL KKDLSVEKI NNNKVEIPK EDEKSTAVK KVEPQLKKN SSHEKINID KVEKPKEDE KSTAVKKVE TQLKKDLSV EKINNNKVD KPKEDEKST AVKKVESQL KKNSSLEKI NNDKVEKSK EDEKSTAVK KVETLLKKD SSLEKVKIN KVEKPKEDE KSTVVKKVE PQLKKNSSL EKINNNKVD KPKEDEKSS IQLKKDLSV EKSDKVEVK SSSIVGKTS SKKEKSDDE LGQQSKIPS KLDNKQPTS QDAAKAPES PKTLKPSLK KTVGEPLKK STSVKIIEE TKKSPAPDE LKKSSSAQK IEETKENEE ENFWAAIGP RESVYTSAR KPLSPPQKP EEKDDELIP AKAMPSKRS LVNKANKDD PDLEVFIPP PEPEPEPEP EPELEPAFV PLQSNRLSQ FMHPFKKPE QFDECPVEI YATPKVIRH RHYPRPRHP PPPVEKSES EEEEEDEES EEETTSSEE TSSSDDEYD VNVVYGNNK VGASTSSND SGFDSGVPG SRNKGSTET RDSPTSNPA NNPALALDS NEVLEEYSH SSTFASFQK SGRITPPAT TIPRFRKYG VEDFNFLKV LGKGSFGKV LLAELKNTE YYYAIKCLK KDVVLEDDD VECTLIERK VLALATKHP YLCHLLCTF QTESHLFFV MEYLNGGDL MFHIQKSGR FPEAQARFY GAEIVSGLK FLHNKGIVY RDLKLDNIL LDFDGHVRI ADFGMCKLQ IFLDRMADT FCGTPDYMA PEIIKGQHY NQCVDWWSF GVLLYEMLL GQSPFSGCD EDELFWSIC NEKPVIPRF LSQEASAVL QLFLEKDGN KRLGNRFCP AGDIQDQPF FKPIQWDKL EARLLEPPF KPNLQHPLD TQYFDKTFT AEKAKLTPI EQNILQSMD QTQFQGFSY TNPNATDK
- >TC034867 RB
MMFTGGSHY KKLTSANSG SGRRGPRKH AEGKSRPSR QTPKAIMVH RNYEGSLGS NSTTSLLKS NSSIESDED DFQRTRGRE ITRRRGAVK HQRVHEVKG HKFLAKFFR QPTFCAFCK DFLWGFCKQ GYQCQACQT AVHKRCHDK LLGKCPESG RESESTIYL RERFKIDLP HRFKIHTFM SPTFCDHCG SLLYGIYKQ GLKCEAGDF YLLASARGM DASNSSYTG SYIPGPYSN HSSLLASNF TRPPPSLLK PRNFRGYKP LLATITENQ SPLRRYNSP KLRGHTSPK VIVAKPIKI NTADIDVSV NKYRKYERP KPVQDKVER KSPSPSDTL DSPRSSEKE AKPNKTIRR DRPTVRLHT VHCDMIAPD NGANTRSWR DNFEPEELK TEEKKPKKT PGELLKEKF LIRSREDIF KAPEKKVSR RASKKTNVQ KTPSFHDIC QAITTDQID EELNPGQPI EIQRRQSRQ FASENLLKD LRRNSGDLT NEVELLNRR NAELSDTRG SVKRRKKLT KKKSNEKVV LTEPDGNKS QLQRRPTQK KIRRTSTSS SVGSDIVDV SPETEKAYQ EAIRDISQV EIENIKIKP KPIITATVD VEEKPALKV VVDEIEVVE SPKPKKGNK FKFNVIVEE IDTKRTSMK KPQTKVEEK VHVPKAKEP LVSNILRKN SNAHLGNIL EEAVNKSEA EVKKSDSAE EMKKVVVVK TISKKEDKE PVQIRKTDS DDKVVVSKE VAQERANQD QTSGQQLKQ SSSVPDMEM KKNEVGKPK EDEKSTSAK KVEAPLKKE LSLDKVKKE DEKSTAAKK AESQLKKDS SIEKITSGN VVKLKEHEE STTVKKIEP ELKKNLSLE KINDKVERP KEDEKSTAM KKVEPQLKK NLSLEKINI DKVEKPKED KPTVVKKVE PQLKKNSSL EKINNDKVE IPKEDEKSM AVKKVEPQL KKDLSVEKI NNNKVEIPK EDEKSTAVK KVEPQLKKN SSHEKINID KVEKPKEDE KSTAVKKVE TQLKKDLSV EKINNNKVD KPKEDEKST AVKKVESQL KKNSSLEKI NNDKVEKSK EDEKSTAVK KVETLLKKD SSLEKVKIN KVEKPKEDE KSTVVKKVE PQLKKNSSL EKINNNKVD KPKEDEKSS IQLKKDLSV EKSDKVEVK SSSIVGKTS SKKEKSDDE LGQQSKIPS KLDNKQPTS QDAAKAPES PKTLKPSLK KTVGEPLKK STSVKIIEE TKKSPAPDE LKKSSSAQK IEETKENEE ENFWAAIGP RESVYTSAR KPLSPPQKP EEKDDELIP AKAMPSKRS LVNKANKDD PDLEVFIPP PEPEPEPEP EPELEPAFV PLQSNRLSQ FMHPFKKPE QFDECPVEI YATPKVIRH RHYPRPRHP PPPVEKSES EEEEEDEES EEETTSSEE TSSSDDEYD VNVVYGNNK VGASTSSND SGFDSGVPG SRNKDCDVN CHKKCEKLT ANLCGVNQK LIVEAIESV RKGSTETRD SPTSNPANN PALALDSNE VLEEYSHSS TFASFQKSG RITPPATTI PRFRKYGVE DFNFLKVLG KGSFGKVLL AELKNTEYY YAIKCLKKD VVLEDDDVE CTLIERKVL ALATKHPYL CHLLCTFQT ESHLFFVME YLNGGDLMF HIQKSGRFP EAQARFYGA EIVSGLKFL HNKGIVYRD LKLDNILLD FDGHVRIAD FGMCKLQIF LDRMADTFC GTPDYMAPE IIKGQHYNQ CVDWWSFGV LLYEMLLGQ SPFSGCDED ELFWSICNE KPVIPRFLS QEASAVLQL FLEKDGNKR LGNRFCPAG DIQDQPFFK PIQWDKLEA RLLEPPFKP NLQHPLDTQ YFDKTFTAE KAKLTPIEQ NILQSMDQT QFQGFSYTN PNATDK
- >TC034867 RA
atgatgttc accggagga tctcactac aagaaactc acttccgcg aactcgggc agtggacga agggggccc cgcaagcat gccgaagga aaatctcgg ccgtcgcgg caaacaccg aaagcaatc atggttcac agaaactac gaaggctca cttggctcc aactcaaca acttcgctg ctgaagagc aacagttcg attgaatcg gacgaagat gacttccaa aggacgcga ggcagggaa attacaagg cgaagaggt gcggtgaag catcaaagg gtacatgag gtgaaagga cacaaattc cttgccaag tttttccgg caaccgact ttttgcgcc ttttgtaaa gacttcctt tgggggttt tgcaaacag gggtaccaa tgtcaagct tgtcaaact gctgtacat aaacgatgc cacgataaa ttgctaggg aagtgtccg gaatcagga cgagaatcc gaaagtaca atatatttg agagaaagg ttcaaaatt gatctgccg cacaggttc aaaatccat acttttatg tctcctaca ttttgtgat cattgtggc tcgttgttg tacgggatc tacaaacaa ggcctaaaa tgtgaagcg ggcgatttt tatttactc gcaagtgca cgtggaatg gacgcatca aacagtagt tacacgggc tcatatata cccggtcct tattccaat cattcctct ttgctggcc tcaaatttc acgaggccg cctccttcc ctgctcaag ccgcgcaat tttcgcggg tacaagccc ctgctcgcg accatcacc gagaaccag tcgccctta aggcggtac aattcgccc aagttgcga gggcacacc tcgcccaaa gttatagtg gccaagccc atcaaaata aacacagct gatatcgac gtttccgtg aacaagtac aggaagtat gagcggcct aagcccgta caagacaaa gttgagagg aaatcgcca agtcccagc gacactcta gacagtcca cggagctca gagaaggaa gcaaagccc aacaaaacc atccgaagg gacaggcca acggttcgc ctccacacc gtccactgt gacatgata gcacccgac aacggtgca aacaccagg agttggagg gacaacttc gagccagaa gaactgaaa accgaggag aagaagccc aaaaagaca cccggtgag ctcctcaag gagaagttt ttgattcgc agtcgggag gacatcttc aaagcgccc gagaagaag gtgagtaga cgggcgtcg aagaaaaca aacgtgcaa aaaacgccg agctttcac gacatctgc caagccata accaccgac caaatcgac gaagagcta aaccctggc cagcccatc gaaatacaa agacgccaa agtcggcaa tttgcaagc gaaaacctc ctcaaagac ctaagacgc aactccggc gatttgacc aacgaagtt gagcttctg aaccggcga aacgccgag ctttctgac acgcgtgga agcgtcaag cggcggaag aaactgacc aagaagaag tccaacgag aaagtggtc ctcaccgaa cctgacggc aacaaatcg cagcttcag cgaagacca actcagaag aaaatccgc cggacttcc accagcagc agcgtgggg agcgacatt gtcgatgtt agtcctgag actgagaag gcgtaccaa gaggcgatt cgtgacatt tcccaggtt gagattgaa aatatcaaa attaagcct aagcctatt attactgca acggttgat gtggaagag aaaccagcc ttgaaggtg gttgtggac gagattgaa gtggtggag tcgccgaag ccgaaaaag ggtaacaag tttaagttt aacgtgatt gttgaggag attgacacg aaacggaca tcgatgaag aagcctcaa accaaagtt gaggaaaag gtgcacgtg ccgaaggcc aaggaacct ttggtgtcg aatattttg aggaagaac agcaatgcg caccttggc aacattttg gaagaagct gttaacaag agtgaagcc gaagtgaag aaaagtgac agtgcggaa gaaatgaag aaagttgta gtggtaaag acaatttcg aagaaagaa gataaggaa cctgttcag atcagaaaa actgacagt gatgataaa gttgtggtg tctaaagaa gttgcacag gaaagagca aatcaagat caaacttct ggtcaacaa ttgaaacaa tcttctagt gttccagat atggaaatg aagaaaaac gaagtagga aaaccaaaa gaagatgaa aaatcaaca tctgcaaag aaagtggaa gctccgtta aagaaagaa ttgagtctc gacaaagtg aaaaaggaa gatgaaaaa tcaacggct gcgaagaaa gctgaatct caactgaag aaagattcg agtatcgag aagattaca agcggcaat gtagtaaaa cttaaagaa catgaggaa tcaacgact gtgaagaag attgaacct gaattgaag aaaaatctg agtcttgaa aagataaac gacaaagta gaaagacca aaagaagat gaaaaatca acggctatg aagaaagtt gaacctcaa ttgaagaaa aatttgagt cttgaaaag ataaacatc gacaaagtt gaaaaacct aaagaagat aaaccaacg gttgtgaag aaagttgaa cctcaattg aagaaaaat tcgagtctt gaaaagata aacaatgac aaagtagag ataccaaaa gaagatgaa aaatcaatg gctgtgaag aaagttgaa cctcaattg aagaaagat ttgagtgtt gaaaagata aacaacaac aaagtagag ataccaaaa gaagatgaa aaatcaacg gctgtgaag aaagttgaa cctcaattg aagaaaaat tcgagtcat gaaaagata aacatcgac aaagtagaa aaaccaaaa gaagatgaa aaatcaacg gctgtgaag aaagttgaa acccagttg aagaaagat ttgagtgtt gaaaagata aacaacaat aaagtagac aaaccaaaa gaagatgaa aaatcaacg gctgtgaag aaagttgaa tctcaattg aagaaaaat tcgagtctt gaaaagata aacaacgac aaagtagaa aaatcgaaa gaagatgaa aaatcaacg gctgtgaag aaagttgaa accctattg aagaaagat tcgagtctt gaaaaggta aaaattaac aaagttgaa aaaccaaaa gaagacgaa aaatcaacc gttgtgaag aaagttgaa cctcaattg aagaaaaat tcgagtctt gagaagata aacaacaac aaagtagac aaaccaaaa gaagatgaa aaatcatca atccagttg aagaaagat ttaagtgtt gaaaaaagt gacaaagtt gaagtcaaa agtagttct atcgttggt aaaaccagt tcaaagaaa gaaaaatca gatgatgag ttaggtcag cagagcaaa attccctca aagcttgat aataaacaa ccaacaagt caagatgcc gccaaagcc ccagaaagt cccaaaaca cttaaacca agcttgaag aaaacagta ggagagcca ctgaaaaaa tcaaccagc gttaaaatt attgaagaa acgaagaaa agtccagct ccagacgaa ctgaaaaaa tcaagcagt gcccaaaaa atcgaagaa acgaaggag aatgaagaa gaaaacttc tgggctgcg attggaccc cgcgaaagc gtctacact tcagcccgg aaaccgtta agtccaccc caaaaaccg gaagaaaag gacgacgag ttaatccca gccaaggca atgccgtca aagcgttca ttggtcaac aaggctaat aaagatgac ccggatttg gaggtgttt atcccgccc ccggagccc gagccggag ccggaaccc gaacccgag ctggaaccc gccttcgtc cctctccag tccaaccgt ttgtcccaa tttatgcac ccgtttaaa aaacccgaa caattcgat gaatgtcct gttgaaatt tacgcgact ccgaaagta atccgccat agacattat ccgcgaccg cgacatccg ccgcccccg gtcgaaaag tccgagagc gaagaagaa gaagaagac gaagaaagt gaagaagaa acaacgtcg agtgaagag acgtcgtca tctgacgat gaatatgac gtcaatgtg gtgtatggc aataacaaa gttggtgca agtacgtct tccaatgac tctggcttt gattcgggc gtccctggc agtagaaat aagggatca accgaaacc cgggactcc ccgacttcc aaccccgca aacaaccct gccctagct ctagactca aacgaagtc ttggaagag tattcccac tccagcact tttgccagc ttccagaaa agtggcaga ataacacca ccagctacg acaattcca cgatttaga aaatacggt gtcgaagac ttcaatttc ctcaaggtt ctgggcaaa ggaagcttc ggaaaggtt ctcttggca gagctgaaa aacaccgaa tattattat gccataaag tgcctgaaa aaagacgtc gttttggaa gacgacgac gttgagtgc accctaatt gaaagaaaa gtcctggct ttggcaacg aaacatcca tatttatgc catttatta tgcaccttc caaaccgag tcacattta ttcttcgtg atggagtat ctaaacggc ggcgacctc atgtttcac atccaaaag tcgggccga tttcccgaa gcccaggcc cgcttctac ggcgctgaa atcgtttca ggcctgaaa tttctccac aacaaaggc attgtttac agggactta aaactcgac aatattttg ttggacttt gacggtcat gtgaggata gcagatttt ggcatgtgc aagcttcag atatttttg gaccgaatg gccgatacg ttttgtggc actcctgat tatatggca cctgaaata attaaagga caacactac aaccagtgt gttgattgg tggtcattc ggtgtactt ttgtacgaa atgttgcta ggtcagtca cctttcagt ggctgtgac gaagacgaa ttattctgg tccatttgt aacgagaaa ccagtgatt ccccggttt ttgtcacaa gaagcgagc gctgttcta caactgttt ttggaaaaa gacggaaac aaacgatta ggaaatagg ttttgccca gctggtgat atccaagac cagccgttt ttcaagccc atccagtgg gacaaactc gaggccagg cttttagaa cctcccttt aaacccaat ttgcaacac ccgttagat acgcagtac tttgacaaa actttcacg gccgaaaaa gccaaattg accccaatc gaacaaaac attttgcaa tcgatggat cagacccag tttcaaggt ttcagttac accaacccc aacgctacg gacaagtga
- >TC034867 RB
atgatgttc accggagga tctcactac aagaaactc acttccgcg aactcgggc agtggacga agggggccc cgcaagcat gccgaagga aaatctcgg ccgtcgcgg caaacaccg aaagcaatc atggttcac agaaactac gaaggctca cttggctcc aactcaaca acttcgctg ctgaagagc aacagttcg attgaatcg gacgaagat gacttccaa aggacgcga ggcagggaa attacaagg cgaagaggt gcggtgaag catcaaagg gtacatgag gtgaaagga cacaaattc cttgccaag tttttccgg caaccgact ttttgcgcc ttttgtaaa gacttcctt tgggggttt tgcaaacag gggtaccaa tgtcaagct tgtcaaact gctgtacat aaacgatgc cacgataaa ttgctaggg aagtgtccg gaatcagga cgagaatcc gaaagtaca atatatttg agagaaagg ttcaaaatt gatctgccg cacaggttc aaaatccat acttttatg tctcctaca ttttgtgat cattgtggc tcgttgttg tacgggatc tacaaacaa ggcctaaaa tgtgaagcg ggcgatttt tatttactc gcaagtgca cgtggaatg gacgcatca aacagtagt tacacgggc tcatatata cccggtcct tattccaat cattcctct ttgctggcc tcaaatttc acgaggccg cctccttcc ctgctcaag ccgcgcaat tttcgcggg tacaagccc ctgctcgcg accatcacc gagaaccag tcgccctta aggcggtac aattcgccc aagttgcga gggcacacc tcgcccaaa gttatagtg gccaagccc atcaaaata aacacagct gatatcgac gtttccgtg aacaagtac aggaagtat gagcggcct aagcccgta caagacaaa gttgagagg aaatcgcca agtcccagc gacactcta gacagtcca cggagctca gagaaggaa gcaaagccc aacaaaacc atccgaagg gacaggcca acggttcgc ctccacacc gtccactgt gacatgata gcacccgac aacggtgca aacaccagg agttggagg gacaacttc gagccagaa gaactgaaa accgaggag aagaagccc aaaaagaca cccggtgag ctcctcaag gagaagttt ttgattcgc agtcgggag gacatcttc aaagcgccc gagaagaag gtgagtaga cgggcgtcg aagaaaaca aacgtgcaa aaaacgccg agctttcac gacatctgc caagccata accaccgac caaatcgac gaagagcta aaccctggc cagcccatc gaaatacaa agacgccaa agtcggcaa tttgcaagc gaaaacctc ctcaaagac ctaagacgc aactccggc gatttgacc aacgaagtt gagcttctg aaccggcga aacgccgag ctttctgac acgcgtgga agcgtcaag cggcggaag aaactgacc aagaagaag tccaacgag aaagtggtc ctcaccgaa cctgacggc aacaaatcg cagcttcag cgaagacca actcagaag aaaatccgc cggacttcc accagcagc agcgtgggg agcgacatt gtcgatgtt agtcctgag actgagaag gcgtaccaa gaggcgatt cgtgacatt tcccaggtt gagattgaa aatatcaaa attaagcct aagcctatt attactgca acggttgat gtggaagag aaaccagcc ttgaaggtg gttgtggac gagattgaa gtggtggag tcgccgaag ccgaaaaag ggtaacaag tttaagttt aacgtgatt gttgaggag attgacacg aaacggaca tcgatgaag aagcctcaa accaaagtt gaggaaaag gtgcacgtg ccgaaggcc aaggaacct ttggtgtcg aatattttg aggaagaac agcaatgcg caccttggc aacattttg gaagaagct gttaacaag agtgaagcc gaagtgaag aaaagtgac agtgcggaa gaaatgaag aaagttgta gtggtaaag acaatttcg aagaaagaa gataaggaa cctgttcag atcagaaaa actgacagt gatgataaa gttgtggtg tctaaagaa gttgcacag gaaagagca aatcaagat caaacttct ggtcaacaa ttgaaacaa tcttctagt gttccagat atggaaatg aagaaaaac gaagtagga aaaccaaaa gaagatgaa aaatcaaca tctgcaaag aaagtggaa gctccgtta aagaaagaa ttgagtctc gacaaagtg aaaaaggaa gatgaaaaa tcaacggct gcgaagaaa gctgaatct caactgaag aaagattcg agtatcgag aagattaca agcggcaat gtagtaaaa cttaaagaa catgaggaa tcaacgact gtgaagaag attgaacct gaattgaag aaaaatctg agtcttgaa aagataaac gacaaagta gaaagacca aaagaagat gaaaaatca acggctatg aagaaagtt gaacctcaa ttgaagaaa aatttgagt cttgaaaag ataaacatc gacaaagtt gaaaaacct aaagaagat aaaccaacg gttgtgaag aaagttgaa cctcaattg aagaaaaat tcgagtctt gaaaagata aacaatgac aaagtagag ataccaaaa gaagatgaa aaatcaatg gctgtgaag aaagttgaa cctcaattg aagaaagat ttgagtgtt gaaaagata aacaacaac aaagtagag ataccaaaa gaagatgaa aaatcaacg gctgtgaag aaagttgaa cctcaattg aagaaaaat tcgagtcat gaaaagata aacatcgac aaagtagaa aaaccaaaa gaagatgaa aaatcaacg gctgtgaag aaagttgaa acccagttg aagaaagat ttgagtgtt gaaaagata aacaacaat aaagtagac aaaccaaaa gaagatgaa aaatcaacg gctgtgaag aaagttgaa tctcaattg aagaaaaat tcgagtctt gaaaagata aacaacgac aaagtagaa aaatcgaaa gaagatgaa aaatcaacg gctgtgaag aaagttgaa accctattg aagaaagat tcgagtctt gaaaaggta aaaattaac aaagttgaa aaaccaaaa gaagacgaa aaatcaacc gttgtgaag aaagttgaa cctcaattg aagaaaaat tcgagtctt gagaagata aacaacaac aaagtagac aaaccaaaa gaagatgaa aaatcatca atccagttg aagaaagat ttaagtgtt gaaaaaagt gacaaagtt gaagtcaaa agtagttct atcgttggt aaaaccagt tcaaagaaa gaaaaatca gatgatgag ttaggtcag cagagcaaa attccctca aagcttgat aataaacaa ccaacaagt caagatgcc gccaaagcc ccagaaagt cccaaaaca cttaaacca agcttgaag aaaacagta ggagagcca ctgaaaaaa tcaaccagc gttaaaatt attgaagaa acgaagaaa agtccagct ccagacgaa ctgaaaaaa tcaagcagt gcccaaaaa atcgaagaa acgaaggag aatgaagaa gaaaacttc tgggctgcg attggaccc cgcgaaagc gtctacact tcagcccgg aaaccgtta agtccaccc caaaaaccg gaagaaaag gacgacgag ttaatccca gccaaggca atgccgtca aagcgttca ttggtcaac aaggctaat aaagatgac ccggatttg gaggtgttt atcccgccc ccggagccc gagccggag ccggaaccc gaacccgag ctggaaccc gccttcgtc cctctccag tccaaccgt ttgtcccaa tttatgcac ccgtttaaa aaacccgaa caattcgat gaatgtcct gttgaaatt tacgcgact ccgaaagta atccgccat agacattat ccgcgaccg cgacatccg ccgcccccg gtcgaaaag tccgagagc gaagaagaa gaagaagac gaagaaagt gaagaagaa acaacgtcg agtgaagag acgtcgtca tctgacgat gaatatgac gtcaatgtg gtgtatggc aataacaaa gttggtgca agtacgtct tccaatgac tctggcttt gattcgggc gtccctggc agtagaaat aaggactgt gatgtaaac tgtcataag aaatgcgag aaactgact gccaatctg tgcggagtt aatcaaaaa ctcattgtc gaggccatc gaatccgtg cgaaaagga tcaaccgaa acccgggac tccccgact tccaacccc gcaaacaac cctgcccta gctctagac tcaaacgaa gtcttggaa gagtattcc cactccagc acttttgcc agcttccag aaaagtggc agaataaca ccaccagct acgacaatt ccacgattt agaaaatac ggtgtcgaa gacttcaat ttcctcaag gttctgggc aaaggaagc ttcggaaag gttctcttg gcagagctg aaaaacacc gaatattat tatgccata aagtgcctg aaaaaagac gtcgttttg gaagacgac gacgttgag tgcacccta attgaaaga aaagtcctg gctttggca acgaaacat ccatattta tgccattta ttatgcacc ttccaaacc gagtcacat ttattcttc gtgatggag tatctaaac ggcggcgac ctcatgttt cacatccaa aagtcgggc cgatttccc gaagcccag gcccgcttc tacggcgct gaaatcgtt tcaggcctg aaatttctc cacaacaaa ggcattgtt tacagggac ttaaaactc gacaatatt ttgttggac tttgacggt catgtgagg atagcagat tttggcatg tgcaagctt cagatattt ttggaccga atggccgat acgttttgt ggcactcct gattatatg gcacctgaa ataattaaa ggacaacac tacaaccag tgtgttgat tggtggtca ttcggtgta cttttgtac gaaatgttg ctaggtcag tcacctttc agtggctgt gacgaagac gaattattc tggtccatt tgtaacgag aaaccagtg attccccgg tttttgtca caagaagcg agcgctgtt ctacaactg tttttggaa aaagacgga aacaaacga ttaggaaat aggttttgc ccagctggt gatatccaa gaccagccg tttttcaag cccatccag tgggacaaa ctcgaggcc aggctttta gaacctccc tttaaaccc aatttgcaa cacccgtta gatacgcag tactttgac aaaactttc acggccgaa aaagccaaa ttgacccca atcgaacaa aacattttg caatcgatg gatcagacc cagtttcaa ggtttcagt tacaccaac cccaacgct acggacaag tga
- >TC034867 RA
gctgcggag taaaatgac gaaaagtac aaaaaccaa gacaaaact gattcattg tgataagac agtctaaag gagcaagaa gatgatgtt caccggagg atctcacta caagaaact cacttccgc gaactcggg cagtggacg aagggggcc ccgcaagca tgccgaagg aaaatctcg gccgtcgcg gcaaacacc gaaagcaat catggttca cagaaacta cgaaggctc acttggctc caactcaac aacttcgct gctgaagag caacagttc gattgaatc ggacgaaga tgacttcca aaggacgcg aggcaggga aattacaag gcgaagagg tgcggtgaa gcatcaaag ggtacatga ggtgaaagg acacaaatt ccttgccaa gtttttccg gcaaccgac tttttgcgc cttttgtaa agacttcct ttgggggtt ttgcaaaca ggggtacca atgtcaagc ttgtcaaac tgctgtaca taaacgatg ccacgataa attgctagg gaagtgtcc ggaatcagg acgagaatc cgaaagtac aatatattt gagagaaag gttcaaaat tgatctgcc gcacaggtt caaaatcca tacttttat gtctcctac attttgtga tcattgtgg ctcgttgtt gtacgggat ctacaaaca aggcctaaa atgtgaagc gggcgattt ttatttact cgcaagtgc acgtggaat ggacgcatc aaacagtag ttacacggg ctcatatat acccggtcc ttattccaa tcattcctc tttgctggc ctcaaattt cacgaggcc gcctccttc cctgctcaa gccgcgcaa ttttcgcgg gtacaagcc cctgctcgc gaccatcac cgagaacca gtcgccctt aaggcggta caattcgcc caagttgcg agggcacac ctcgcccaa agttatagt ggccaagcc catcaaaat aaacacagc tgatatcga cgtttccgt gaacaagta caggaagta tgagcggcc taagcccgt acaagacaa agttgagag gaaatcgcc aagtcccag cgacactct agacagtcc acggagctc agagaagga agcaaagcc caacaaaac catccgaag ggacaggcc aacggttcg cctccacac cgtccactg tgacatgat agcacccga caacggtgc aaacaccag gagttggag ggacaactt cgagccaga agaactgaa aaccgagga gaagaagcc caaaaagac acccggtga gctcctcaa ggagaagtt tttgattcg cagtcggga ggacatctt caaagcgcc cgagaagaa ggtgagtag acgggcgtc gaagaaaac aaacgtgca aaaaacgcc gagctttca cgacatctg ccaagccat aaccaccga ccaaatcga cgaagagct aaaccctgg ccagcccat cgaaataca aagacgcca aagtcggca atttgcaag cgaaaacct cctcaaaga cctaagacg caactccgg cgatttgac caacgaagt tgagcttct gaaccggcg aaacgccga gctttctga cacgcgtgg aagcgtcaa gcggcggaa gaaactgac caagaagaa gtccaacga gaaagtggt cctcaccga acctgacgg caacaaatc gcagcttca gcgaagacc aactcagaa gaaaatccg ccggacttc caccagcag cagcgtggg gagcgacat tgtcgatgt tagtcctga gactgagaa ggcgtacca agaggcgat tcgtgacat ttcccaggt tgagattga aaatatcaa aattaagcc taagcctat tattactgc aacggttga tgtggaaga gaaaccagc cttgaaggt ggttgtgga cgagattga agtggtgga gtcgccgaa gccgaaaaa gggtaacaa gtttaagtt taacgtgat tgttgagga gattgacac gaaacggac atcgatgaa gaagcctca aaccaaagt tgaggaaaa ggtgcacgt gccgaaggc caaggaacc tttggtgtc gaatatttt gaggaagaa cagcaatgc gcaccttgg caacatttt ggaagaagc tgttaacaa gagtgaagc cgaagtgaa gaaaagtga cagtgcgga agaaatgaa gaaagttgt agtggtaaa gacaatttc gaagaaaga agataagga acctgttca gatcagaaa aactgacag tgatgataa agttgtggt gtctaaaga agttgcaca ggaaagagc aaatcaaga tcaaacttc tggtcaaca attgaaaca atcttctag tgttccaga tatggaaat gaagaaaaa cgaagtagg aaaaccaaa agaagatga aaaatcaac atctgcaaa gaaagtgga agctccgtt aaagaaaga attgagtct cgacaaagt gaaaaagga agatgaaaa atcaacggc tgcgaagaa agctgaatc tcaactgaa gaaagattc gagtatcga gaagattac aagcggcaa tgtagtaaa acttaaaga acatgagga atcaacgac tgtgaagaa gattgaacc tgaattgaa gaaaaatct gagtcttga aaagataaa cgacaaagt agaaagacc aaaagaaga tgaaaaatc aacggctat gaagaaagt tgaacctca attgaagaa aaatttgag tcttgaaaa gataaacat cgacaaagt tgaaaaacc taaagaaga taaaccaac ggttgtgaa gaaagttga acctcaatt gaagaaaaa ttcgagtct tgaaaagat aaacaatga caaagtaga gataccaaa agaagatga aaaatcaat ggctgtgaa gaaagttga acctcaatt gaagaaaga tttgagtgt tgaaaagat aaacaacaa caaagtaga gataccaaa agaagatga aaaatcaac ggctgtgaa gaaagttga acctcaatt gaagaaaaa ttcgagtca tgaaaagat aaacatcga caaagtaga aaaaccaaa agaagatga aaaatcaac ggctgtgaa gaaagttga aacccagtt gaagaaaga tttgagtgt tgaaaagat aaacaacaa taaagtaga caaaccaaa agaagatga aaaatcaac ggctgtgaa gaaagttga atctcaatt gaagaaaaa ttcgagtct tgaaaagat aaacaacga caaagtaga aaaatcgaa agaagatga aaaatcaac ggctgtgaa gaaagttga aaccctatt gaagaaaga ttcgagtct tgaaaaggt aaaaattaa caaagttga aaaaccaaa agaagacga aaaatcaac cgttgtgaa gaaagttga acctcaatt gaagaaaaa ttcgagtct tgagaagat aaacaacaa caaagtaga caaaccaaa agaagatga aaaatcatc aatccagtt gaagaaaga tttaagtgt tgaaaaaag tgacaaagt tgaagtcaa aagtagttc tatcgttgg taaaaccag ttcaaagaa agaaaaatc agatgatga gttaggtca gcagagcaa aattccctc aaagcttga taataaaca accaacaag tcaagatgc cgccaaagc cccagaaag tcccaaaac acttaaacc aagcttgaa gaaaacagt aggagagcc actgaaaaa atcaaccag cgttaaaat tattgaaga aacgaagaa aagtccagc tccagacga actgaaaaa atcaagcag tgcccaaaa aatcgaaga aacgaagga gaatgaaga agaaaactt ctgggctgc gattggacc ccgcgaaag cgtctacac ttcagcccg gaaaccgtt aagtccacc ccaaaaacc ggaagaaaa ggacgacga gttaatccc agccaaggc aatgccgtc aaagcgttc attggtcaa caaggctaa taaagatga cccggattt ggaggtgtt tatcccgcc cccggagcc cgagccgga gccggaacc cgaacccga gctggaacc cgccttcgt ccctctcca gtccaaccg tttgtccca atttatgca cccgtttaa aaaacccga acaattcga tgaatgtcc tgttgaaat ttacgcgac tccgaaagt aatccgcca tagacatta tccgcgacc gcgacatcc gccgccccc ggtcgaaaa gtccgagag cgaagaaga agaagaaga cgaagaaag tgaagaaga aacaacgtc gagtgaaga gacgtcgtc atctgacga tgaatatga cgtcaatgt ggtgtatgg caataacaa agttggtgc aagtacgtc ttccaatga ctctggctt tgattcggg cgtccctgg cagtagaaa taagggatc aaccgaaac ccgggactc cccgacttc caaccccgc aaacaaccc tgccctagc tctagactc aaacgaagt cttggaaga gtattccca ctccagcac ttttgccag cttccagaa aagtggcag aataacacc accagctac gacaattcc acgatttag aaaatacgg tgtcgaaga cttcaattt cctcaaggt tctgggcaa aggaagctt cggaaaggt tctcttggc agagctgaa aaacaccga atattatta tgccataaa gtgcctgaa aaaagacgt cgttttgga agacgacga cgttgagtg caccctaat tgaaagaaa agtcctggc tttggcaac gaaacatcc atatttatg ccatttatt atgcacctt ccaaaccga gtcacattt attcttcgt gatggagta tctaaacgg cggcgacct catgtttca catccaaaa gtcgggccg atttcccga agcccaggc ccgcttcta cggcgctga aatcgtttc aggcctgaa atttctcca caacaaagg cattgttta cagggactt aaaactcga caatatttt gttggactt tgacggtca tgtgaggat agcagattt tggcatgtg caagcttca gatattttt ggaccgaat ggccgatac gttttgtgg cactcctga ttatatggc acctgaaat aattaaagg acaacacta caaccagtg tgttgattg gtggtcatt cggtgtact tttgtacga aatgttgct aggtcagtc acctttcag tggctgtga cgaagacga attattctg gtccatttg taacgagaa accagtgat tccccggtt tttgtcaca agaagcgag cgctgttct acaactgtt tttggaaaa agacggaaa caaacgatt aggaaatag gttttgccc agctggtga tatccaaga ccagccgtt tttcaagcc catccagtg ggacaaact cgaggccag gcttttaga acctccctt taaacccaa tttgcaaca cccgttaga tacgcagta ctttgacaa aactttcac ggccgaaaa agccaaatt gaccccaat cgaacaaaa cattttgca atcgatgga tcagaccca gtttcaagg tttcagtta caccaaccc caacgctac ggacaagtg agccagctt gtgcaaaaa ttagcaaag tcggttcgt ctcgttaag agacataat ctggtggtt gattaatgt tcctatgtg attgaattt cgccccaag actttcgga atgaaagtg ccacc
- >TC034867 RB
gctgcggag taaaatgac gaaaagtac aaaaaccaa gacaaaact gattcattg tgataagac agtctaaag gagcaagaa gatgatgtt caccggagg atctcacta caagaaact cacttccgc gaactcggg cagtggacg aagggggcc ccgcaagca tgccgaagg aaaatctcg gccgtcgcg gcaaacacc gaaagcaat catggttca cagaaacta cgaaggctc acttggctc caactcaac aacttcgct gctgaagag caacagttc gattgaatc ggacgaaga tgacttcca aaggacgcg aggcaggga aattacaag gcgaagagg tgcggtgaa gcatcaaag ggtacatga ggtgaaagg acacaaatt ccttgccaa gtttttccg gcaaccgac tttttgcgc cttttgtaa agacttcct ttgggggtt ttgcaaaca ggggtacca atgtcaagc ttgtcaaac tgctgtaca taaacgatg ccacgataa attgctagg gaagtgtcc ggaatcagg acgagaatc cgaaagtac aatatattt gagagaaag gttcaaaat tgatctgcc gcacaggtt caaaatcca tacttttat gtctcctac attttgtga tcattgtgg ctcgttgtt gtacgggat ctacaaaca aggcctaaa atgtgaagc gggcgattt ttatttact cgcaagtgc acgtggaat ggacgcatc aaacagtag ttacacggg ctcatatat acccggtcc ttattccaa tcattcctc tttgctggc ctcaaattt cacgaggcc gcctccttc cctgctcaa gccgcgcaa ttttcgcgg gtacaagcc cctgctcgc gaccatcac cgagaacca gtcgccctt aaggcggta caattcgcc caagttgcg agggcacac ctcgcccaa agttatagt ggccaagcc catcaaaat aaacacagc tgatatcga cgtttccgt gaacaagta caggaagta tgagcggcc taagcccgt acaagacaa agttgagag gaaatcgcc aagtcccag cgacactct agacagtcc acggagctc agagaagga agcaaagcc caacaaaac catccgaag ggacaggcc aacggttcg cctccacac cgtccactg tgacatgat agcacccga caacggtgc aaacaccag gagttggag ggacaactt cgagccaga agaactgaa aaccgagga gaagaagcc caaaaagac acccggtga gctcctcaa ggagaagtt tttgattcg cagtcggga ggacatctt caaagcgcc cgagaagaa ggtgagtag acgggcgtc gaagaaaac aaacgtgca aaaaacgcc gagctttca cgacatctg ccaagccat aaccaccga ccaaatcga cgaagagct aaaccctgg ccagcccat cgaaataca aagacgcca aagtcggca atttgcaag cgaaaacct cctcaaaga cctaagacg caactccgg cgatttgac caacgaagt tgagcttct gaaccggcg aaacgccga gctttctga cacgcgtgg aagcgtcaa gcggcggaa gaaactgac caagaagaa gtccaacga gaaagtggt cctcaccga acctgacgg caacaaatc gcagcttca gcgaagacc aactcagaa gaaaatccg ccggacttc caccagcag cagcgtggg gagcgacat tgtcgatgt tagtcctga gactgagaa ggcgtacca agaggcgat tcgtgacat ttcccaggt tgagattga aaatatcaa aattaagcc taagcctat tattactgc aacggttga tgtggaaga gaaaccagc cttgaaggt ggttgtgga cgagattga agtggtgga gtcgccgaa gccgaaaaa gggtaacaa gtttaagtt taacgtgat tgttgagga gattgacac gaaacggac atcgatgaa gaagcctca aaccaaagt tgaggaaaa ggtgcacgt gccgaaggc caaggaacc tttggtgtc gaatatttt gaggaagaa cagcaatgc gcaccttgg caacatttt ggaagaagc tgttaacaa gagtgaagc cgaagtgaa gaaaagtga cagtgcgga agaaatgaa gaaagttgt agtggtaaa gacaatttc gaagaaaga agataagga acctgttca gatcagaaa aactgacag tgatgataa agttgtggt gtctaaaga agttgcaca ggaaagagc aaatcaaga tcaaacttc tggtcaaca attgaaaca atcttctag tgttccaga tatggaaat gaagaaaaa cgaagtagg aaaaccaaa agaagatga aaaatcaac atctgcaaa gaaagtgga agctccgtt aaagaaaga attgagtct cgacaaagt gaaaaagga agatgaaaa atcaacggc tgcgaagaa agctgaatc tcaactgaa gaaagattc gagtatcga gaagattac aagcggcaa tgtagtaaa acttaaaga acatgagga atcaacgac tgtgaagaa gattgaacc tgaattgaa gaaaaatct gagtcttga aaagataaa cgacaaagt agaaagacc aaaagaaga tgaaaaatc aacggctat gaagaaagt tgaacctca attgaagaa aaatttgag tcttgaaaa gataaacat cgacaaagt tgaaaaacc taaagaaga taaaccaac ggttgtgaa gaaagttga acctcaatt gaagaaaaa ttcgagtct tgaaaagat aaacaatga caaagtaga gataccaaa agaagatga aaaatcaat ggctgtgaa gaaagttga acctcaatt gaagaaaga tttgagtgt tgaaaagat aaacaacaa caaagtaga gataccaaa agaagatga aaaatcaac ggctgtgaa gaaagttga acctcaatt gaagaaaaa ttcgagtca tgaaaagat aaacatcga caaagtaga aaaaccaaa agaagatga aaaatcaac ggctgtgaa gaaagttga aacccagtt gaagaaaga tttgagtgt tgaaaagat aaacaacaa taaagtaga caaaccaaa agaagatga aaaatcaac ggctgtgaa gaaagttga atctcaatt gaagaaaaa ttcgagtct tgaaaagat aaacaacga caaagtaga aaaatcgaa agaagatga aaaatcaac ggctgtgaa gaaagttga aaccctatt gaagaaaga ttcgagtct tgaaaaggt aaaaattaa caaagttga aaaaccaaa agaagacga aaaatcaac cgttgtgaa gaaagttga acctcaatt gaagaaaaa ttcgagtct tgagaagat aaacaacaa caaagtaga caaaccaaa agaagatga aaaatcatc aatccagtt gaagaaaga tttaagtgt tgaaaaaag tgacaaagt tgaagtcaa aagtagttc tatcgttgg taaaaccag ttcaaagaa agaaaaatc agatgatga gttaggtca gcagagcaa aattccctc aaagcttga taataaaca accaacaag tcaagatgc cgccaaagc cccagaaag tcccaaaac acttaaacc aagcttgaa gaaaacagt aggagagcc actgaaaaa atcaaccag cgttaaaat tattgaaga aacgaagaa aagtccagc tccagacga actgaaaaa atcaagcag tgcccaaaa aatcgaaga aacgaagga gaatgaaga agaaaactt ctgggctgc gattggacc ccgcgaaag cgtctacac ttcagcccg gaaaccgtt aagtccacc ccaaaaacc ggaagaaaa ggacgacga gttaatccc agccaaggc aatgccgtc aaagcgttc attggtcaa caaggctaa taaagatga cccggattt ggaggtgtt tatcccgcc cccggagcc cgagccgga gccggaacc cgaacccga gctggaacc cgccttcgt ccctctcca gtccaaccg tttgtccca atttatgca cccgtttaa aaaacccga acaattcga tgaatgtcc tgttgaaat ttacgcgac tccgaaagt aatccgcca tagacatta tccgcgacc gcgacatcc gccgccccc ggtcgaaaa gtccgagag cgaagaaga agaagaaga cgaagaaag tgaagaaga aacaacgtc gagtgaaga gacgtcgtc atctgacga tgaatatga cgtcaatgt ggtgtatgg caataacaa agttggtgc aagtacgtc ttccaatga ctctggctt tgattcggg cgtccctgg cagtagaaa taaggactg tgatgtaaa ctgtcataa gaaatgcga gaaactgac tgccaatct gtgcggagt taatcaaaa actcattgt cgaggccat cgaatccgt gcgaaaagg atcaaccga aacccggga ctccccgac ttccaaccc cgcaaacaa ccctgccct agctctaga ctcaaacga agtcttgga agagtattc ccactccag cacttttgc cagcttcca gaaaagtgg cagaataac accaccagc tacgacaat tccacgatt tagaaaata cggtgtcga agacttcaa tttcctcaa ggttctggg caaaggaag cttcggaaa ggttctctt ggcagagct gaaaaacac cgaatatta ttatgccat aaagtgcct gaaaaaaga cgtcgtttt ggaagacga cgacgttga gtgcaccct aattgaaag aaaagtcct ggctttggc aacgaaaca tccatattt atgccattt attatgcac cttccaaac cgagtcaca tttattctt cgtgatgga gtatctaaa cggcggcga cctcatgtt tcacatcca aaagtcggg ccgatttcc cgaagccca ggcccgctt ctacggcgc tgaaatcgt ttcaggcct gaaatttct ccacaacaa aggcattgt ttacaggga cttaaaact cgacaatat tttgttgga ctttgacgg tcatgtgag gatagcaga ttttggcat gtgcaagct tcagatatt tttggaccg aatggccga tacgttttg tggcactcc tgattatat ggcacctga aataattaa aggacaaca ctacaacca gtgtgttga ttggtggtc attcggtgt acttttgta cgaaatgtt gctaggtca gtcaccttt cagtggctg tgacgaaga cgaattatt ctggtccat ttgtaacga gaaaccagt gattccccg gtttttgtc acaagaagc gagcgctgt tctacaact gtttttgga aaaagacgg aaacaaacg attaggaaa taggttttg cccagctgg tgatatcca agaccagcc gtttttcaa gcccatcca gtgggacaa actcgaggc caggctttt agaacctcc ctttaaacc caatttgca acacccgtt agatacgca gtactttga caaaacttt cacggccga aaaagccaa attgacccc aatcgaaca aaacatttt gcaatcgat ggatcagac ccagtttca aggtttcag ttacaccaa ccccaacgc tacggacaa gtgagccag cttgtgcaa aaattagca aagtcggtt cgtctcgtt aagagacat aatctggtg gttgattaa tgttcctat gtgattgaa tttcgcccc aagactttc ggaatgaaa gtgccacc
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