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Gene information
General information
TC034311 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 5801333..5807095
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034311
    iBeetle screen iB_05170

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034311 RA
      MQKGCWKVD DNDEGTVSN TFYKRDKHH KFSHELWTE 
      MYKSKNTGS KAVYIDLED EEQRDEDRQ YSQWSELPD 
      LLLEKIFSY LSIREKYYA SLVCKSWYR AFHLPYVWS 
      QFVLEDNTL TRGRFNYYS GWQYVLDHL RTQNCLSQV 
      GRNFRKLTF EPMLNFYNL YEFMNMVSW CTEQSQNND 
      NPEFGVGKN IHTLKFTFP CNMTNRDDT ERIRLFGTG 
      GKLLAALKR LMSNLKKLK RLELIDLML DPKEAQFLL 
      DEVCESCCM TLNTLSVIN TTRVQYELF HVGVFLNLQ 
      VLYISPQNL GDDIIELLS HTKIRHIHI LQNRYTPND 
      INMKPVDFK TWKLARKNN PELRVHLQI ESLKEKPLI 
      WQQGAPVST VLYDSPHIM LREDALMTA IEFYKDTLR 
      VFGHKNIPR FHRNKSFYE RVDSSLMLL VRQCLHLNT 
      LIVTERIST ATALLLAYT GKNLKCLFI RGNAVIIRC 
      DWPQNPDWS DEFYTWLKI NSRSYELVE QEISQIMGR 
      RWQFLSDKQ FKKLHCNLH EF
    CDS sequence
    • >TC034311 RA
      atgcaaaaa ggctgttgg aaagttgat gacaatgac 
      gaaggtact gtatcgaac acattttac aaacgcgac 
      aaacaccac aaattctct cacgagtta tggaccgaa 
      atgtacaag tccaaaaac acgggctcg aaagccgtt 
      tacatcgac ctggaggac gaagagcag agagacgag 
      gaccgacag tactcccaa tggtccgaa ctccccgac 
      ctcctcctg gagaaaatc ttctcctac ctgtcaatc 
      cgcgagaaa tactacgcg agtctggtg tgcaagtcc 
      tggtacagg gccttccac ctgccctac gtgtggtcg 
      cagttcgtc ctagaagac aacactctg acccgtggc 
      cgattcaac tactactcc ggctggcag tacgtcctg 
      gaccacttg cggacgcag aactgcctc tcgcaggtc 
      ggccgcaac ttccgcaaa ctgacgttc gagcccatg 
      ctgaacttc tacaacctg tacgagttc atgaacatg 
      gtgtcctgg tgcacggaa cagtcgcag aacaacgac 
      aacccggag tttggggtc ggcaagaac atccacact 
      ctgaagttc accttcccg tgcaatatg accaacagg 
      gacgatacc gagaggatc cgacttttc ggaaccggg 
      ggcaagctg ttagcggca ctaaagcgt ctgatgtcc 
      aatttgaag aaactgaag cgactggaa ttgattgat 
      ttgatgttg gaccccaag gaagcgcag tttttgcta 
      gacgaagtt tgtgagagc tgctgcatg acgctgaac 
      accttgtca gtgattaac acgacgagg gtgcagtat 
      gagctgttt cacgtcggg gtttttctc aatttgcag 
      gttttgtac atcagtccg caaaacttg ggcgacgac 
      ataatcgag cttctcagc cacacgaaa ataagacac 
      attcatatt ttacaaaac aggtacacc cccaacgac 
      atcaacatg aaaccagtg gacttcaag acgtggaaa 
      ctggcccga aaaaataat cccgaattg agagtccac 
      ttacagatc gaaagtctc aaagaaaaa ccgctgatt 
      tggcaacag ggggctcct gtcagtaca gtcctgtac 
      gattcgccc cacattatg ctgagagag gatgcctta 
      atgactgcg attgagttc tacaaagac acgctccga 
      gtctttggc cacaaaaat attccgcgg ttccaccga 
      aacaagtcg ttttacgag cgcgttgac agtagtttg 
      atgctgctc gtgcggcaa tgtctccac ttgaatact 
      ttaattgtt actgaacga atttcgacc gcaacggct 
      ctgctgctg gcctacacc ggtaaaaat ttgaagtgt 
      ctgtttatt agaggaaac gctgtgata atacgttgt 
      gattggccg caaaatccc gactggagc gacgagttc 
      tacacctgg cttaaaatc aacagccgc tcgtacgag 
      ttagtagag caggaaatc agccaaatt atgggacgg 
      agatggcaa tttttgagt gacaaacaa tttaagaag 
      ctccactgc aatttgcac gagttctga
    mRNA sequence
    • >TC034311 RA
      tatttacaa accgaaaaa cagaatgca aaaaggctg 
      ttggaaagt tgatgacaa tgacgaagg tactgtatc 
      gaacacatt ttacaaacg cgacaaaca ccacaaatt 
      ctctcacga gttatggac cgaaatgta caagtccaa 
      aaacacggg ctcgaaagc cgtttacat cgacctgga 
      ggacgaaga gcagagaga cgaggaccg acagtactc 
      ccaatggtc cgaactccc cgacctcct cctggagaa 
      aatcttctc ctacctgtc aatccgcga gaaatacta 
      cgcgagtct ggtgtgcaa gtcctggta cagggcctt 
      ccacctgcc ctacgtgtg gtcgcagtt cgtcctaga 
      agacaacac tctgacccg tggccgatt caactacta 
      ctccggctg gcagtacgt cctggacca cttgcggac 
      gcagaactg cctctcgca ggtcggccg caacttccg 
      caaactgac gttcgagcc catgctgaa cttctacaa 
      cctgtacga gttcatgaa catggtgtc ctggtgcac 
      ggaacagtc gcagaacaa cgacaaccc ggagtttgg 
      ggtcggcaa gaacatcca cactctgaa gttcacctt 
      cccgtgcaa tatgaccaa cagggacga taccgagag 
      gatccgact tttcggaac cgggggcaa gctgttagc 
      ggcactaaa gcgtctgat gtccaattt gaagaaact 
      gaagcgact ggaattgat tgatttgat gttggaccc 
      caaggaagc gcagttttt gctagacga agtttgtga 
      gagctgctg catgacgct gaacacctt gtcagtgat 
      taacacgac gagggtgca gtatgagct gtttcacgt 
      cggggtttt tctcaattt gcaggtttt gtacatcag 
      tccgcaaaa cttgggcga cgacataat cgagcttct 
      cagccacac gaaaataag acacattca tattttaca 
      aaacaggta cacccccaa cgacatcaa catgaaacc 
      agtggactt caagacgtg gaaactggc ccgaaaaaa 
      taatcccga attgagagt ccacttaca gatcgaaag 
      tctcaaaga aaaaccgct gatttggca acagggggc 
      tcctgtcag tacagtcct gtacgattc gccccacat 
      tatgctgag agaggatgc cttaatgac tgcgattga 
      gttctacaa agacacgct ccgagtctt tggccacaa 
      aaatattcc gcggttcca ccgaaacaa gtcgtttta 
      cgagcgcgt tgacagtag tttgatgct gctcgtgcg 
      gcaatgtct ccacttgaa tactttaat tgttactga 
      acgaatttc gaccgcaac ggctctgct gctggccta 
      caccggtaa aaatttgaa gtgtctgtt tattagagg 
      aaacgctgt gataatacg ttgtgattg gccgcaaaa 
      tcccgactg gagcgacga gttctacac ctggcttaa 
      aatcaacag ccgctcgta cgagttagt agagcagga 
      aatcagcca aattatggg acggagatg gcaattttt 
      gagtgacaa acaatttaa gaagctcca ctgcaattt 
      gcacgagtt ctgaatttt tttttgtat cgcttgtta 
      cttattttg cattttggt ttatttttt ggtaagttt 
      acagaatgt tacgtaaat aataaacat tgatttgtt 
      ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY