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Gene information
General information
TC012080 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6343906..6404955
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012080
    iBeetle screen iB_05164

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012080 RA
      MSFNEGKML PPSFEDFVT DYVLASLAM NNNNNSSTN 
      NNNSNSNNN NLLSSSQQN NAKIEQEQP DSDTIKMFV 
      GQVPRSMDE NDLRRMFEE YGRVHSINV LRDKTTGAS 
      KGCCFVTFF TRKAALQAQ DALHNVKTL NGMHHPIQM 
      KPADSENRN ERKLFVGML SKKLCENDV RTLFSGYGT 
      IEECTVLRD TAGNSKGCA FVTFASKQS ALSAIKALH 
      QSQTMEGCS APLVVKFAD TQKEKELKR QQQIQANVW 
      NALAAPQLQ TSPQQFSPV LSNDATSLQ LLQAMSGGS 
      ALLPQQLLT GAENLLAPL GVQNLVTLA AMSQPTAAP 
      LCVANLLGK GAGVERTLT TGLQTGMST SDLSTYGSL 
      ITNATLNAA AIAAAGKQI EGPDGCNLF IYHLPQEFT 
      DTDLASTFL PFGPVISAK VFIDKQTNL SKCFGFVSF 
      DNATSAQQA IAAMNGFQI GTKRLKVQL KRAKDASKP 
      Y
    CDS sequence
    • >TC012080 RA
      atgagtttt aacgaagga aaaatgttg ccgccgtct 
      ttcgaggat ttcgtgaca gattacgta ctcgcctca 
      ctggcgatg aacaacaac aacaacagc agtacgaac 
      aacaacaac agcaatagc aacaacaac aatttgctg 
      agttcgtcg caacaaaac aatgccaaa atcgaacaa 
      gagcaaccg gacagtgat acgatcaag atgttcgtg 
      gggcaggtg ccccggtcc atggacgaa aacgatctc 
      agaaggatg tttgaagaa tacggacgc gtacactcg 
      atcaacgtc cttagggac aaaaccacg ggagccagt 
      aaaggttgt tgcttcgtg acgttcttt acaagaaag 
      gcagctctt caggcgcag gacgcttta cacaatgtg 
      aaaacctta aacgggatg catcatccc atccagatg 
      aagcccgcc gacagcgag aatagaaac gaaagaaaa 
      ttgttcgtg ggcatgctc agcaagaag ctgtgcgag 
      aacgacgtc cgaaccctc ttcagcggc tacggcacc 
      atcgaggag tgcaccgtg ctgcgggat acggccggg 
      aattcgaaa gggtgcgcg ttcgtcacg ttcgccagc 
      aaacagtca gctcttagt gccataaaa gccctccat 
      cagtcgcaa acgatggaa gggtgttcg gctcctcta 
      gtcgtcaaa ttcgccgac acacaaaaa gaaaaagaa 
      ctgaaacgg cagcagcag atccaggcg aatgtctgg 
      aacgcgttg gcggcgcct cagctgcag acgtctccg 
      caacagttc agtccggtc ctgtcgaac gacgcgacg 
      tccctgcaa ctgttgcag gcgatgagc ggcggctcg 
      gcgcttctg ccgcagcag cttttaacc ggcgccgag 
      aacctgttg gcgcctttg ggggtgcaa aacttggtc 
      acgttggcg gcgatgtcg caaccgacc gccgccccc 
      ttgtgtgta gctaatctg ttaggcaaa ggtgcgggt 
      gttgaaaga actttgacc accggactt cagacgggc 
      atgtccacg tcggacttg tccacctat gggtcgctt 
      atcaccaat gctacgctc aacgccgcc gccatcgcg 
      gcagcagga aagcaaatt gaaggtccg gacggttgc 
      aatttgttt atctaccac ctgccgcag gaatttacc 
      gatacggac ttggcgtcg acgttcctg ccgttcggt 
      cctgtgatc tctgcgaag gtgttcata gacaaacaa 
      acgaatttg tcgaagtgt ttcggtttc gtgtcgttc 
      gacaacgcg acgtcggcc cagcaggcc atcgccgcg 
      atgaacggg ttccagata ggcaccaag cggctcaag 
      gtgcagctg aagagggcc aaagacgcg tccaagccc 
      tactag
    mRNA sequence
    • >TC012080 RA
      ataaagacg aatttagct tatttcgag aatggttca 
      aagggtgcg aaattaaaa tttggcgga cgctgaaga 
      gctcgcatg cgcttcttg gttttttct ttggaccca 
      acgataagc ccatatcgc ggtgtgatt ttcgtgtgt 
      gcaagactc gtgttttat gtttcccga atttttaga 
      aacgatgag ttttaacga aggaaaaat gttgccgcc 
      gtctttcga ggatttcgt gacagatta cgtactcgc 
      ctcactggc gatgaacaa caacaacaa cagcagtac 
      gaacaacaa caacagcaa tagcaacaa caacaattt 
      gctgagttc gtcgcaaca aaacaatgc caaaatcga 
      acaagagca accggacag tgatacgat caagatgtt 
      cgtggggca ggtgccccg gtccatgga cgaaaacga 
      tctcagaag gatgtttga agaatacgg acgcgtaca 
      ctcgatcaa cgtccttag ggacaaaac cacgggagc 
      cagtaaagg ttgttgctt cgtgacgtt ctttacaag 
      aaaggcagc tcttcaggc gcaggacgc tttacacaa 
      tgtgaaaac cttaaacgg gatgcatca tcccatcca 
      gatgaagcc cgccgacag cgagaatag aaacgaaag 
      aaaattgtt cgtgggcat gctcagcaa gaagctgtg 
      cgagaacga cgtccgaac cctcttcag cggctacgg 
      caccatcga ggagtgcac cgtgctgcg ggatacggc 
      cgggaattc gaaagggtg cgcgttcgt cacgttcgc 
      cagcaaaca gtcagctct tagtgccat aaaagccct 
      ccatcagtc gcaaacgat ggaagggtg ttcggctcc 
      tctagtcgt caaattcgc cgacacaca aaaagaaaa 
      agaactgaa acggcagca gcagatcca ggcgaatgt 
      ctggaacgc gttggcggc gcctcagct gcagacgtc 
      tccgcaaca gttcagtcc ggtcctgtc gaacgacgc 
      gacgtccct gcaactgtt gcaggcgat gagcggcgg 
      ctcggcgct tctgccgca gcagctttt aaccggcgc 
      cgagaacct gttggcgcc tttgggggt gcaaaactt 
      ggtcacgtt ggcggcgat gtcgcaacc gaccgccgc 
      ccccttgtg tgtagctaa tctgttagg caaaggtgc 
      gggtgttga aagaacttt gaccaccgg acttcagac 
      gggcatgtc cacgtcgga cttgtccac ctatgggtc 
      gcttatcac caatgctac gctcaacgc cgccgccat 
      cgcggcagc aggaaagca aattgaagg tccggacgg 
      ttgcaattt gtttatcta ccacctgcc gcaggaatt 
      taccgatac ggacttggc gtcgacgtt cctgccgtt 
      cggtcctgt gatctctgc gaaggtgtt catagacaa 
      acaaacgaa tttgtcgaa gtgtttcgg tttcgtgtc 
      gttcgacaa cgcgacgtc ggcccagca ggccatcgc 
      cgcgatgaa cgggttcca gataggcac caagcggct 
      caaggtgca gctgaagag ggccaaaga cgcgtccaa 
      gccctacta gccaaagag acagccagt cgtactcgt 
      attcacaag tacaaccac cagcacagt gccagtgtc 
      ttgtgccca tctcaggat atcgtctag ctgctattt 
      atacgaact gtgagatat ttttactag ttaactgcg 
      gcggtctcg ctgcttgta aattgataa atgattatg 
      tatttgacg aatgacgag gatgttcgg caacaataa 
      gtgttatgt ttctattat tactgtcat gtttggttc 
      gataggttt tgtaagttg tgtttcgtg gcgttcgcg 
      cgtagacat cctctgttg tttagttct atcgtctag 
      gccataaat gtaaatgtt gagtgatta tttcgttta 
      gttctgagt ctactttat attatcatt tatttatat 
      gcgcatgta tttgatgaa ttgtacctt tattttcta 
      ttgttgatt aaaccaaag ttagaccaa acacccgac 
      caatgtctt taatattat gcagggtct tattacact 
      cacgcccaa gccaaacag acaggaccc ttaaaatga 
      taaagctaa cccgctttc gctacgaaa ttaaatgtc 
      tcccctaac aacacaatt actcttccg ccatacgaa 
      gacaaatac ataacttcg tgtttttac atgagggtt 
      tgtgtgcct gcctgataa cagtattga tcacctgtc 
      tggttggtg atgggttaa ctgtagtag cattttacc 
      gcagctgtt cttagtgct gttgtaata ccacctagt 
      gtctaccag tactactgt gtctttctg gcgcggtcc 
      agcagtgcc gtcgcgtgt ggcttctgt ctatgtcta 
      ctaatgtta atgtcttca ttgaagtaa gtggttctc 
      atctgacta acactagct ctctaaccc cctaatctc 
      aagcaccct tcacacaac acacgaggt tccgaagca 
      ccgtgttgg actaactgt tactgactc tactaaatc 
      agcgaaaat ataccaaaa agtttattt acgaaacat 
      atgatttcc tatacataa ttattgata gttgtaagc 
      aaacaaaac aaaaatgca tacgtccct gaaacgaac 
      acatttttc tattttttg aacgtccaa acatcggaa 
      atgcatcag cacaataaa cacgcattt ccaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY