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Gene information
General information
TC012072 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6706476..6748593
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012072
    iBeetle screen iB_05162

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012072 RA
      MGANQSGRM EKTFYGEEV NFDHFQILR AIGKGSFGK 
      VCIVQKKDS KQMFAMKYM NKHQCMERD ALKNVLREV 
      EILTRLEHP FLVNIWFSF QDEEDLFMV TDLLLGGDL 
      RYHIQQEVN FSEDAVKLM VCELALALD YLQTKQIIH 
      RDIKPDNIL LDEEGHFHI TDFNIATVL EDTHLATSM 
      SGTKPYIAP EIFDCAVDL CVGYSYPVD WWSLGVVAY 
      EMLRGLRPF DIHSNTGIQ EVRILFQIG LDYPPSWSE 
      GIVDLISRL LCISPGLRI SNIQELKQV KCLKKFDME 
      RVFQRQYKP YFTPPKDHL NCDPTFELE EMIIETKPL 
      HKKKKRLAK QRSIREIQG PMSIDLDAD SPLELPGTI 
      IPDFKVYNR YQELEKRER ERKEQAWER ELELAMRSS 
      DPLKEETEP KEKIHKCTG CPKLETMIN SNCKLNYDD 
      RGSNMSQLS LPDTKHDDT PVSDIQNKL QNIDFIDRT 
      PSPVERTEG T
    CDS sequence
    • >TC012072 RA
      atgggggcc aaccagtcg ggaaggatg gaaaaaacg 
      ttttatggg gaagaagtg aatttcgac cattttcaa 
      attttacgg gcgatagga aaaggaagc ttcggcaag 
      gtgtgcatc gtgcagaag aaggactcg aaacagatg 
      tttgcgatg aagtacatg aataagcat cagtgtatg 
      gagcgggac gccctcaag aacgtactc agagaggtg 
      gaaattctg acgagatta gaacatccc tttcttgtc 
      aatatttgg ttttcattt caagatgaa gaagatctc 
      ttcatggtg accgatctg ctgctgggg ggcgacctc 
      aggtaccat atccagcaa gaagtgaac ttctcggag 
      gatgccgtc aaactgatg gtgtgcgag ctggccctg 
      gccctggat tatctccaa acaaagcag ataatacac 
      agggatatc aagccggac aacattctg ttggacgaa 
      gaaggacat ttccacatc acggacttt aacatagcc 
      acagttttg gaagacact catttggcg acgtctatg 
      tcggggact aagccgtac atagccccg gaaatcttc 
      gactgtgcc gtggattta tgcgtggga tattcttat 
      cccgtggac tggtggtcg ctgggcgtg gtggcttat 
      gagatgttg aggggcctg agaccgttt gatattcac 
      tccaatacc ggcatacaa gaagtgcga atcttattc 
      cagatcgga ttagattac ccgccgagt tggagcgaa 
      gggatcgtt gatttaatt tcaaggttg ctgtgtata 
      agtccgggc ttgcggata tccaacata caagaactc 
      aaacaagtt aagtgccta aagaagttc gacatggag 
      agagtgttt cagcggcaa tacaagccc tactttacg 
      ccccctaaa gaccatctg aattgtgac ccgaccttc 
      gagctcgag gaaatgatc attgagaca aaacccctg 
      cacaagaaa aagaagcga ttggcgaaa cagcgatcg 
      attagagag attcaaggg ccgatgagc atagatctg 
      gatgccgac tctccgctg gagcttccg gggactata 
      atccccgat ttcaaggtt tataacagg tatcaagag 
      ttggaaaaa agggagcgt gaaagaaag gaacaggcg 
      tgggagagg gaactcgag ctggcaatg cgatcctct 
      gatcctctc aaggaggaa acggagccc aaggaaaaa 
      atccacaaa tgcacaggc tgtcccaaa ttagaaaca 
      atgatcaat tccaactgc aaactcaac tacgacgac 
      cgtggaagt aacatgagt caattatcg ttacccgac 
      acgaaacat gacgacact cccgttagc gacatacaa 
      aacaaactg caaaatata gacttcatc gacaggaca 
      ccatcaccg gtagaaaga accgagggc acttaa
    mRNA sequence
    • >TC012072 RA
      caagtcggc aaaattcgc atttcaagc ctagatacc 
      caataactg gatcagtct caatgttac aaattttgt 
      cctttttcc atgcaaatg aggaaatga tccgatgaa 
      ttattcata attcatgag acgcatcgg gttataatc 
      cttaatttg ctatgcgaa aaaccgtca ccctttggg 
      aaaaatcca agtaggacg cggtttaga aaattcagc 
      cgacattag caaaattgc ttaatccag tggtcattt 
      tttaattat tgtaaacag cgggtttgg aatgaattg 
      cgtgattag gcggggaaa accgacgcc ggccgatgt 
      ttcgacgct gtaagacgc cgacgacgc accgattta 
      gtcgtttgg tgcttttca aggacttag gttgggcat 
      tttcgcttt gtggggcac acgtggaca cgatatata 
      ttccagtca actgaaaat tatgaaaac atggatgta 
      tcctaaata ttcaactac gatgtaact agttttaaa 
      tgtagaagt tttggcgaa taaaatcaa ctacaatgg 
      gggccaacc agtcgggaa ggatggaaa aaacgtttt 
      atggggaag aagtgaatt tcgaccatt ttcaaattt 
      tacgggcga taggaaaag gaagcttcg gcaaggtgt 
      gcatcgtgc agaagaagg actcgaaac agatgtttg 
      cgatgaagt acatgaata agcatcagt gtatggagc 
      gggacgccc tcaagaacg tactcagag aggtggaaa 
      ttctgacga gattagaac atccctttc ttgtcaata 
      tttggtttt catttcaag atgaagaag atctcttca 
      tggtgaccg atctgctgc tggggggcg acctcaggt 
      accatatcc agcaagaag tgaacttct cggaggatg 
      ccgtcaaac tgatggtgt gcgagctgg ccctggccc 
      tggattatc tccaaacaa agcagataa tacacaggg 
      atatcaagc cggacaaca ttctgttgg acgaagaag 
      gacatttcc acatcacgg actttaaca tagccacag 
      ttttggaag acactcatt tggcgacgt ctatgtcgg 
      ggactaagc cgtacatag ccccggaaa tcttcgact 
      gtgccgtgg atttatgcg tgggatatt cttatcccg 
      tggactggt ggtcgctgg gcgtggtgg cttatgaga 
      tgttgaggg gcctgagac cgtttgata ttcactcca 
      ataccggca tacaagaag tgcgaatct tattccaga 
      tcggattag attacccgc cgagttgga gcgaaggga 
      tcgttgatt taatttcaa ggttgctgt gtataagtc 
      cgggcttgc ggatatcca acatacaag aactcaaac 
      aagttaagt gcctaaaga agttcgaca tggagagag 
      tgtttcagc ggcaataca agccctact ttacgcccc 
      ctaaagacc atctgaatt gtgacccga ccttcgagc 
      tcgaggaaa tgatcattg agacaaaac ccctgcaca 
      agaaaaaga agcgattgg cgaaacagc gatcgatta 
      gagagattc aagggccga tgagcatag atctggatg 
      ccgactctc cgctggagc ttccgggga ctataatcc 
      ccgatttca aggtttata acaggtatc aagagttgg 
      aaaaaaggg agcgtgaaa gaaaggaac aggcgtggg 
      agagggaac tcgagctgg caatgcgat cctctgatc 
      ctctcaagg aggaaacgg agcccaagg aaaaaatcc 
      acaaatgca caggctgtc ccaaattag aaacaatga 
      tcaattcca actgcaaac tcaactacg acgaccgtg 
      gaagtaaca tgagtcaat tatcgttac ccgacacga 
      aacatgacg acactcccg ttagcgaca tacaaaaca 
      aactgcaaa atatagact tcatcgaca ggacaccat 
      caccggtag aaagaaccg agggcactt aacttttta 
      taaccagga caaatgtat attttttaa taaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY