| General information | |||
| TC012067 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 6773069..6782531 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC012067 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_05161 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (19 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 5 (22 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (22 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (41 dpi)
- >TC012067 RA
MSDLFKSAY EYFSGPTNG QSDNNFVGQ VIEISNVKL RIKKVIAEG GFAVVFVAQ DVATGKDYA LKRLLAADE EAKNNIMQE INILKKLAG HPNIIQYLS ASFIDKSQT THGKAEFLL VTELCTGGS LAEILQARS APFEPEIIT RIFYQTCRA LAHMHSQNP PIIHRDLKL ENLLISRDG TIKLCDFGS ATVEIFRPD LTWSANQHD SLEENMAHC TTPMYRAPE MVDTWNNYF VGPPVDIWA LGCLLYVFC YMKHPFEDS AKLRIINAN YNLPPDPKY SCFQDIIRG CLQVNPEQR LTVSGVLER VAAIAESNG FNLRAPLQF QQKQSPEPD HSSGDGVHN NVKPPPRPV PPKPQPAPE RPPQPPQRP AMPVQRPPE GKQSGLFSS LKGGAGSFL KNLKDTSSK VMATMQQSI ARTDLDVSY VTSRILVMP YPSEGIESA YKTNHIEDV RVFLDSRHP NLKYSVYNL SGKSYHSKF GQARVVDCT FAYPEHFKA PLLNSLYQL CEDIYQYLA GDSRNVVVV HCTDGKATS ATLVCGLLI YSFLYEVPE DALQMFAVK RCPPNMRPS ELRYLYYLA DIVKNPPAY PHYKPVTFV SIQMQPVPL FTKIRDGCR PYLEVYSEN RCLLSTLQD YEKMRLYNV TEGKVLLPI NVSVCGDVC ITIYHARNI LGGVMTQGK ATGIKICQL QLHTGFIPE EETCLKFNK SELDEISDG QDHYQDRFM VLLNVFVSD SERKPSQPP PWLTDKTQR VCDTMFSSK LEQDETIDN FASKQPKRV QVSPQKPSE RPNRPAPPS PRPAHVELE QKDSSDSEA EQVKPTPKP VEPPLIEAV DLLNLNTTP NPSTIKPSA SSNFDLLSG LDSSNDNFP SFPSNPAPN PQPSLFDPF GSSDPQNNL LGDWGNFNS APPTKPPEP QPQKPTDFF SDLGNLGGG AWNSNASSP IKVPPTTPQ QGGTPQHKP VTPQHQAKS PDYSRSHFD AFGDKGEKG KPKSDDVFG DLLGSQGYK FSAKKDNAP RTINDMRKE DMATYMDPD KIKIMEWKE GKKNNIRAL LCSLHTVLW EGTKWNKCD MSQLVTPAD VKKAYRKAC LAVHPDKQT GTDNENMAK LIFMELNNA WSDFENDAS QQNIFTS
- >TC012067 RA
atgagtgac ttatttaaa tcagcatat gagtacttt agtggcccc acgaacggg caaagtgac aacaatttc gtgggccaa gtaatagaa atttctaat gttaaactc cgaataaaa aaagtcatt gctgaaggc ggttttgct gtagttttc gttgcccag gacgtggcc acgggcaaa gactacgct ttgaaaaga cttttggcc gcagatgaa gaagcaaag aacaacatt atgcaggag attaacatc ctgaagaag ctggcaggg caccccaac attatccag tatttgtcg gcgtcgttt attgataaa tcacagacc acacatggg aaggcggag tttttgttg gtcacagag ttgtgcaca ggaggctcc ttggccgag attttgcag gcgaggagt gcccccttc gagccggaa atcatcaca cgaatattc tatcaaaca tgtcgggct ttggcacac atgcactcg cagaatccg ccaattata cacagagac ttaaaacta gagaattta ttgataagt agagacggt acaataaaa ctatgtgac tttggttca gccactgtt gaaatattt agacctgac ctgacctgg tctgccaac caacacgac agtctggaa gaaaatatg gcgcactgt acaaccccc atgtaccgc gcccccgaa atggtcgac acttggaac aactatttc gtgggcccc cctgtggac atctgggcc ctgggctgt ctcctctac gtcttttgt tacatgaag cacccgttt gaagacagc gctaaattg cgaattatt aacgctaat tacaatctt cctcccgat cctaaatat tcatgcttc caagacatt atccgtggc tgtctccaa gtcaatccc gagcaacgc ttgaccgtg tccggagtg ctggagcgg gtggctgcc atcgccgag tccaacggt ttcaatttg agggccccc ttgcagttc cagcagaag cagtccccg gagcccgac cacagctcc ggcgatggg gtccacaac aacgtgaag ccgccgccg cggccggtg ccccccaag ccgcagccg gcccccgag cggcccccg cagcccccc cagaggccc gcaatgccc gtgcagcgg cccccggag gggaagcag tcggggctg ttctcgtcg ctgaagggc ggagccggg agttttttg aagaatttg aaagatacg agttcgaaa gtgatggca acgatgcaa caatcgata gcgaggacg gatttggat gtgagttat gtgacttcg cgtattttg gtgatgccg tatccgtct gagggtatt gaatctgcg tataaaact aatcatatt gaagatgtg cgcgtgttt ttggactcg aggcatccg aatttgaag tattccgtt tataatctt agcgggaag tcgtatcat tcgaagttt gggcaagcg agggttgtc gattgtacg tttgcgtat cctgaacat tttaaagct ccgttgttg aattcgctg taccagctt tgcgaggat atttatcag tatttggct ggtgatagt cggaatgtg gtcgttgtt cactgcacg gacggaaaa gccacctct gccaccctc gtatgcggc ttacttatt tattcattt ctttacgaa gttcctgaa gatgcccta caaatgttt gccgtcaag cgctgtcca cccaatatg agaccttcc gaattaaga tacctgtac tatttggca gatattgtg aaaaacccg ccggcttat ccgcactat aaaccggtc acttttgtc tcgattcag atgcagccg gtcccactt ttcaccaaa attagagat gggtgtcgc ccgtatttg gaggtttac agcgagaat cggtgctta ctttcgacg ctacaagac tacgaaaaa atgagattg tataatgtt accgaagga aaagtttta ttgccgatc aatgtgagc gtttgtggc gacgtttgc atcaccatt taccacgca agaaacatc ctagggggc gtcatgacc caaggcaag gctacaggg attaaaatc tgccaactg caattacac accggtttt ataccggag gaggaaacc tgtttgaaa tttaacaaa tcggaattg gatgaaata tccgacggt caggatcac taccaggac agatttatg gttctttta aacgttttt gtgtccgat tcggaacga aaaccctca cagccgccc ccatggttg acggacaag acgcaacgt gtttgtgat actatgttt agttcgaag ttagaacaa gacgagacg atagataat tttgccagt aaacaacca aagcgggtg caagtgtca ccccagaag ccttcagag cgtccgaat aggcctgcg cccccgtcc cccaggccc gcacacgta gaactagaa cagaaagat tccagcgat tccgaggcc gagcaagtc aagccaacg cctaagcca gtggagccc cccttaatc gaagccgtc gacttactc aacctcaac accaccccc aacccctcg accataaag ccctccgca agctcaaac ttcgatctc ttaagcggt ttggattcc tccaacgac aacttcccc tcatttccc tcgaatccc gctcccaac ccccagccg tcgctcttt gacccgttc ggttcctcg gacccccaa aataacctc ctcggcgat tggggtaat ttcaattcg gcccctccg actaagccc cccgagccc cagccccag aaaccgacc gactttttc tccgacttg ggcaatctg ggcggaggt gcgtggaac agcaacgcg tcctcgccc atcaaagtc ccccccacg accccccaa caagggggc accccccaa cacaagccc gtcacgccg caacaccaa gccaagtcg ccggattac agcagatcc cactttgat gcgttcggc gataagggc gagaaaggg aagccgaag agcgatgat gtctttggg gatttgctg gggtcgcag gggtacaag ttcagcgcg aagaaagac aacgcccct aggacgatc aacgatatg aggaaagag gatatggcg acttacatg gaccctgat aagattaaa atcatggaa tggaaagag ggcaagaag aacaatatc agggcgttg ctgtgttcg ctacatacg gtcctgtgg gaagggacg aagtggaat aagtgcgat atgtcgcag ctggtgaca cccgccgat gttaagaag gcgtacagg aaggcctgt ttggcggtg catccggat aagcaaacg ggtaccgat aacgagaac atggccaag ctgattttt atggaattg aacaatgcc tggagtgac ttcgaaaat gatgcctcc caacaaaat atcttcact agctaa
- >TC012067 RA
acacaagtt tggcaacac ttgtctggg ggcgtgtgg gggtgtact tcggcgaaa aaatcccta aacaaatca tttttatcg agggttttg ttatgagtt tcctcgaat tttccacgt aaaatgagt gacttattt aaatcagca tatgagtac tttagtggc cccacgaac gggcaaagt gacaacaat ttcgtgggc caagtaata gaaatttct aatgttaaa ctccgaata aaaaaagtc attgctgaa ggcggtttt gctgtagtt ttcgttgcc caggacgtg gccacgggc aaagactac gctttgaaa agacttttg gccgcagat gaagaagca aagaacaac attatgcag gagattaac atcctgaag aagctggca gggcacccc aacattatc cagtatttg tcggcgtcg tttattgat aaatcacag accacacat gggaaggcg gagtttttg ttggtcaca gagttgtgc acaggaggc tccttggcc gagattttg caggcgagg agtgccccc ttcgagccg gaaatcatc acacgaata ttctatcaa acatgtcgg gctttggca cacatgcac tcgcagaat ccgccaatt atacacaga gacttaaaa ctagagaat ttattgata agtagagac ggtacaata aaactatgt gactttggt tcagccact gttgaaata tttagacct gacctgacc tggtctgcc aaccaacac gacagtctg gaagaaaat atggcgcac tgtacaacc cccatgtac cgcgccccc gaaatggtc gacacttgg aacaactat ttcgtgggc ccccctgtg gacatctgg gccctgggc tgtctcctc tacgtcttt tgttacatg aagcacccg tttgaagac agcgctaaa ttgcgaatt attaacgct aattacaat cttcctccc gatcctaaa tattcatgc ttccaagac attatccgt ggctgtctc caagtcaat cccgagcaa cgcttgacc gtgtccgga gtgctggag cgggtggct gccatcgcc gagtccaac ggtttcaat ttgagggcc cccttgcag ttccagcag aagcagtcc ccggagccc gaccacagc tccggcgat ggggtccac aacaacgtg aagccgccg ccgcggccg gtgcccccc aagccgcag ccggccccc gagcggccc ccgcagccc ccccagagg cccgcaatg cccgtgcag cggcccccg gaggggaag cagtcgggg ctgttctcg tcgctgaag ggcggagcc gggagtttt ttgaagaat ttgaaagat acgagttcg aaagtgatg gcaacgatg caacaatcg atagcgagg acggatttg gatgtgagt tatgtgact tcgcgtatt ttggtgatg ccgtatccg tctgagggt attgaatct gcgtataaa actaatcat attgaagat gtgcgcgtg tttttggac tcgaggcat ccgaatttg aagtattcc gtttataat cttagcggg aagtcgtat cattcgaag tttgggcaa gcgagggtt gtcgattgt acgtttgcg tatcctgaa cattttaaa gctccgttg ttgaattcg ctgtaccag ctttgcgag gatatttat cagtatttg gctggtgat agtcggaat gtggtcgtt gttcactgc acggacgga aaagccacc tctgccacc ctcgtatgc ggcttactt atttattca tttctttac gaagttcct gaagatgcc ctacaaatg tttgccgtc aagcgctgt ccacccaat atgagacct tccgaatta agatacctg tactatttg gcagatatt gtgaaaaac ccgccggct tatccgcac tataaaccg gtcactttt gtctcgatt cagatgcag ccggtccca cttttcacc aaaattaga gatgggtgt cgcccgtat ttggaggtt tacagcgag aatcggtgc ttactttcg acgctacaa gactacgaa aaaatgaga ttgtataat gttaccgaa ggaaaagtt ttattgccg atcaatgtg agcgtttgt ggcgacgtt tgcatcacc atttaccac gcaagaaac atcctaggg ggcgtcatg acccaaggc aaggctaca gggattaaa atctgccaa ctgcaatta cacaccggt tttataccg gaggaggaa acctgtttg aaatttaac aaatcggaa ttggatgaa atatccgac ggtcaggat cactaccag gacagattt atggttctt ttaaacgtt tttgtgtcc gattcggaa cgaaaaccc tcacagccg cccccatgg ttgacggac aagacgcaa cgtgtttgt gatactatg tttagttcg aagttagaa caagacgag acgatagat aattttgcc agtaaacaa ccaaagcgg gtgcaagtg tcaccccag aagccttca gagcgtccg aataggcct gcgcccccg tcccccagg cccgcacac gtagaacta gaacagaaa gattccagc gattccgag gccgagcaa gtcaagcca acgcctaag ccagtggag cccccctta atcgaagcc gtcgactta ctcaacctc aacaccacc cccaacccc tcgaccata aagccctcc gcaagctca aacttcgat ctcttaagc ggtttggat tcctccaac gacaacttc ccctcattt ccctcgaat cccgctccc aacccccag ccgtcgctc tttgacccg ttcggttcc tcggacccc caaaataac ctcctcggc gattggggt aatttcaat tcggcccct ccgactaag ccccccgag ccccagccc cagaaaccg accgacttt ttctccgac ttgggcaat ctgggcgga ggtgcgtgg aacagcaac gcgtcctcg cccatcaaa gtccccccc acgaccccc caacaaggg ggcaccccc caacacaag cccgtcacg ccgcaacac caagccaag tcgccggat tacagcaga tcccacttt gatgcgttc ggcgataag ggcgagaaa gggaagccg aagagcgat gatgtcttt ggggatttg ctggggtcg caggggtac aagttcagc gcgaagaaa gacaacgcc cctaggacg atcaacgat atgaggaaa gaggatatg gcgacttac atggaccct gataagatt aaaatcatg gaatggaaa gagggcaag aagaacaat atcagggcg ttgctgtgt tcgctacat acggtcctg tgggaaggg acgaagtgg aataagtgc gatatgtcg cagctggtg acacccgcc gatgttaag aaggcgtac aggaaggcc tgtttggcg gtgcatccg gataagcaa acgggtacc gataacgag aacatggcc aagctgatt tttatggaa ttgaacaat gcctggagt gacttcgaa aatgatgcc tcccaacaa aatatcttc actagctaa ttcttctct actatgtga tcattccgt tttatggtt aacttatag attatttat tttaggtga ttttatttc aattttaca taacaatga tgtagctct aaagtgatg actttatca tatgaaatg aaagcaatt gtcggatct agacaatat tttaatcgt agttacttt agaggtgtt gttaaaatt gagcgctga tgataatga tattatact taatagttt tgttttgta ttgtttaga atatatttt tatattgtg tcactgtta cataacgtc atttgtgca attcatgat tacgattcg tttccagtg ttttaaatt ttattcaag ttgcaaact tcaaaaata tatctcaaa gagcaat
