Status
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Gene information
General information
TC012067 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6773069..6782531
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012067
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_05161

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 5 (22 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012067 RA
      MSDLFKSAY EYFSGPTNG QSDNNFVGQ VIEISNVKL 
      RIKKVIAEG GFAVVFVAQ DVATGKDYA LKRLLAADE 
      EAKNNIMQE INILKKLAG HPNIIQYLS ASFIDKSQT 
      THGKAEFLL VTELCTGGS LAEILQARS APFEPEIIT 
      RIFYQTCRA LAHMHSQNP PIIHRDLKL ENLLISRDG 
      TIKLCDFGS ATVEIFRPD LTWSANQHD SLEENMAHC 
      TTPMYRAPE MVDTWNNYF VGPPVDIWA LGCLLYVFC 
      YMKHPFEDS AKLRIINAN YNLPPDPKY SCFQDIIRG 
      CLQVNPEQR LTVSGVLER VAAIAESNG FNLRAPLQF 
      QQKQSPEPD HSSGDGVHN NVKPPPRPV PPKPQPAPE 
      RPPQPPQRP AMPVQRPPE GKQSGLFSS LKGGAGSFL 
      KNLKDTSSK VMATMQQSI ARTDLDVSY VTSRILVMP 
      YPSEGIESA YKTNHIEDV RVFLDSRHP NLKYSVYNL 
      SGKSYHSKF GQARVVDCT FAYPEHFKA PLLNSLYQL 
      CEDIYQYLA GDSRNVVVV HCTDGKATS ATLVCGLLI 
      YSFLYEVPE DALQMFAVK RCPPNMRPS ELRYLYYLA 
      DIVKNPPAY PHYKPVTFV SIQMQPVPL FTKIRDGCR 
      PYLEVYSEN RCLLSTLQD YEKMRLYNV TEGKVLLPI 
      NVSVCGDVC ITIYHARNI LGGVMTQGK ATGIKICQL 
      QLHTGFIPE EETCLKFNK SELDEISDG QDHYQDRFM 
      VLLNVFVSD SERKPSQPP PWLTDKTQR VCDTMFSSK 
      LEQDETIDN FASKQPKRV QVSPQKPSE RPNRPAPPS 
      PRPAHVELE QKDSSDSEA EQVKPTPKP VEPPLIEAV 
      DLLNLNTTP NPSTIKPSA SSNFDLLSG LDSSNDNFP 
      SFPSNPAPN PQPSLFDPF GSSDPQNNL LGDWGNFNS 
      APPTKPPEP QPQKPTDFF SDLGNLGGG AWNSNASSP 
      IKVPPTTPQ QGGTPQHKP VTPQHQAKS PDYSRSHFD 
      AFGDKGEKG KPKSDDVFG DLLGSQGYK FSAKKDNAP 
      RTINDMRKE DMATYMDPD KIKIMEWKE GKKNNIRAL 
      LCSLHTVLW EGTKWNKCD MSQLVTPAD VKKAYRKAC 
      LAVHPDKQT GTDNENMAK LIFMELNNA WSDFENDAS 
      QQNIFTS
    CDS sequence
    • >TC012067 RA
      atgagtgac ttatttaaa tcagcatat gagtacttt 
      agtggcccc acgaacggg caaagtgac aacaatttc 
      gtgggccaa gtaatagaa atttctaat gttaaactc 
      cgaataaaa aaagtcatt gctgaaggc ggttttgct 
      gtagttttc gttgcccag gacgtggcc acgggcaaa 
      gactacgct ttgaaaaga cttttggcc gcagatgaa 
      gaagcaaag aacaacatt atgcaggag attaacatc 
      ctgaagaag ctggcaggg caccccaac attatccag 
      tatttgtcg gcgtcgttt attgataaa tcacagacc 
      acacatggg aaggcggag tttttgttg gtcacagag 
      ttgtgcaca ggaggctcc ttggccgag attttgcag 
      gcgaggagt gcccccttc gagccggaa atcatcaca 
      cgaatattc tatcaaaca tgtcgggct ttggcacac 
      atgcactcg cagaatccg ccaattata cacagagac 
      ttaaaacta gagaattta ttgataagt agagacggt 
      acaataaaa ctatgtgac tttggttca gccactgtt 
      gaaatattt agacctgac ctgacctgg tctgccaac 
      caacacgac agtctggaa gaaaatatg gcgcactgt 
      acaaccccc atgtaccgc gcccccgaa atggtcgac 
      acttggaac aactatttc gtgggcccc cctgtggac 
      atctgggcc ctgggctgt ctcctctac gtcttttgt 
      tacatgaag cacccgttt gaagacagc gctaaattg 
      cgaattatt aacgctaat tacaatctt cctcccgat 
      cctaaatat tcatgcttc caagacatt atccgtggc 
      tgtctccaa gtcaatccc gagcaacgc ttgaccgtg 
      tccggagtg ctggagcgg gtggctgcc atcgccgag 
      tccaacggt ttcaatttg agggccccc ttgcagttc 
      cagcagaag cagtccccg gagcccgac cacagctcc 
      ggcgatggg gtccacaac aacgtgaag ccgccgccg 
      cggccggtg ccccccaag ccgcagccg gcccccgag 
      cggcccccg cagcccccc cagaggccc gcaatgccc 
      gtgcagcgg cccccggag gggaagcag tcggggctg 
      ttctcgtcg ctgaagggc ggagccggg agttttttg 
      aagaatttg aaagatacg agttcgaaa gtgatggca 
      acgatgcaa caatcgata gcgaggacg gatttggat 
      gtgagttat gtgacttcg cgtattttg gtgatgccg 
      tatccgtct gagggtatt gaatctgcg tataaaact 
      aatcatatt gaagatgtg cgcgtgttt ttggactcg 
      aggcatccg aatttgaag tattccgtt tataatctt 
      agcgggaag tcgtatcat tcgaagttt gggcaagcg 
      agggttgtc gattgtacg tttgcgtat cctgaacat 
      tttaaagct ccgttgttg aattcgctg taccagctt 
      tgcgaggat atttatcag tatttggct ggtgatagt 
      cggaatgtg gtcgttgtt cactgcacg gacggaaaa 
      gccacctct gccaccctc gtatgcggc ttacttatt 
      tattcattt ctttacgaa gttcctgaa gatgcccta 
      caaatgttt gccgtcaag cgctgtcca cccaatatg 
      agaccttcc gaattaaga tacctgtac tatttggca 
      gatattgtg aaaaacccg ccggcttat ccgcactat 
      aaaccggtc acttttgtc tcgattcag atgcagccg 
      gtcccactt ttcaccaaa attagagat gggtgtcgc 
      ccgtatttg gaggtttac agcgagaat cggtgctta 
      ctttcgacg ctacaagac tacgaaaaa atgagattg 
      tataatgtt accgaagga aaagtttta ttgccgatc 
      aatgtgagc gtttgtggc gacgtttgc atcaccatt 
      taccacgca agaaacatc ctagggggc gtcatgacc 
      caaggcaag gctacaggg attaaaatc tgccaactg 
      caattacac accggtttt ataccggag gaggaaacc 
      tgtttgaaa tttaacaaa tcggaattg gatgaaata 
      tccgacggt caggatcac taccaggac agatttatg 
      gttctttta aacgttttt gtgtccgat tcggaacga 
      aaaccctca cagccgccc ccatggttg acggacaag 
      acgcaacgt gtttgtgat actatgttt agttcgaag 
      ttagaacaa gacgagacg atagataat tttgccagt 
      aaacaacca aagcgggtg caagtgtca ccccagaag 
      ccttcagag cgtccgaat aggcctgcg cccccgtcc 
      cccaggccc gcacacgta gaactagaa cagaaagat 
      tccagcgat tccgaggcc gagcaagtc aagccaacg 
      cctaagcca gtggagccc cccttaatc gaagccgtc 
      gacttactc aacctcaac accaccccc aacccctcg 
      accataaag ccctccgca agctcaaac ttcgatctc 
      ttaagcggt ttggattcc tccaacgac aacttcccc 
      tcatttccc tcgaatccc gctcccaac ccccagccg 
      tcgctcttt gacccgttc ggttcctcg gacccccaa 
      aataacctc ctcggcgat tggggtaat ttcaattcg 
      gcccctccg actaagccc cccgagccc cagccccag 
      aaaccgacc gactttttc tccgacttg ggcaatctg 
      ggcggaggt gcgtggaac agcaacgcg tcctcgccc 
      atcaaagtc ccccccacg accccccaa caagggggc 
      accccccaa cacaagccc gtcacgccg caacaccaa 
      gccaagtcg ccggattac agcagatcc cactttgat 
      gcgttcggc gataagggc gagaaaggg aagccgaag 
      agcgatgat gtctttggg gatttgctg gggtcgcag 
      gggtacaag ttcagcgcg aagaaagac aacgcccct 
      aggacgatc aacgatatg aggaaagag gatatggcg 
      acttacatg gaccctgat aagattaaa atcatggaa 
      tggaaagag ggcaagaag aacaatatc agggcgttg 
      ctgtgttcg ctacatacg gtcctgtgg gaagggacg 
      aagtggaat aagtgcgat atgtcgcag ctggtgaca 
      cccgccgat gttaagaag gcgtacagg aaggcctgt 
      ttggcggtg catccggat aagcaaacg ggtaccgat 
      aacgagaac atggccaag ctgattttt atggaattg 
      aacaatgcc tggagtgac ttcgaaaat gatgcctcc 
      caacaaaat atcttcact agctaa
    mRNA sequence
    • >TC012067 RA
      acacaagtt tggcaacac ttgtctggg ggcgtgtgg 
      gggtgtact tcggcgaaa aaatcccta aacaaatca 
      tttttatcg agggttttg ttatgagtt tcctcgaat 
      tttccacgt aaaatgagt gacttattt aaatcagca 
      tatgagtac tttagtggc cccacgaac gggcaaagt 
      gacaacaat ttcgtgggc caagtaata gaaatttct 
      aatgttaaa ctccgaata aaaaaagtc attgctgaa 
      ggcggtttt gctgtagtt ttcgttgcc caggacgtg 
      gccacgggc aaagactac gctttgaaa agacttttg 
      gccgcagat gaagaagca aagaacaac attatgcag 
      gagattaac atcctgaag aagctggca gggcacccc 
      aacattatc cagtatttg tcggcgtcg tttattgat 
      aaatcacag accacacat gggaaggcg gagtttttg 
      ttggtcaca gagttgtgc acaggaggc tccttggcc 
      gagattttg caggcgagg agtgccccc ttcgagccg 
      gaaatcatc acacgaata ttctatcaa acatgtcgg 
      gctttggca cacatgcac tcgcagaat ccgccaatt 
      atacacaga gacttaaaa ctagagaat ttattgata 
      agtagagac ggtacaata aaactatgt gactttggt 
      tcagccact gttgaaata tttagacct gacctgacc 
      tggtctgcc aaccaacac gacagtctg gaagaaaat 
      atggcgcac tgtacaacc cccatgtac cgcgccccc 
      gaaatggtc gacacttgg aacaactat ttcgtgggc 
      ccccctgtg gacatctgg gccctgggc tgtctcctc 
      tacgtcttt tgttacatg aagcacccg tttgaagac 
      agcgctaaa ttgcgaatt attaacgct aattacaat 
      cttcctccc gatcctaaa tattcatgc ttccaagac 
      attatccgt ggctgtctc caagtcaat cccgagcaa 
      cgcttgacc gtgtccgga gtgctggag cgggtggct 
      gccatcgcc gagtccaac ggtttcaat ttgagggcc 
      cccttgcag ttccagcag aagcagtcc ccggagccc 
      gaccacagc tccggcgat ggggtccac aacaacgtg 
      aagccgccg ccgcggccg gtgcccccc aagccgcag 
      ccggccccc gagcggccc ccgcagccc ccccagagg 
      cccgcaatg cccgtgcag cggcccccg gaggggaag 
      cagtcgggg ctgttctcg tcgctgaag ggcggagcc 
      gggagtttt ttgaagaat ttgaaagat acgagttcg 
      aaagtgatg gcaacgatg caacaatcg atagcgagg 
      acggatttg gatgtgagt tatgtgact tcgcgtatt 
      ttggtgatg ccgtatccg tctgagggt attgaatct 
      gcgtataaa actaatcat attgaagat gtgcgcgtg 
      tttttggac tcgaggcat ccgaatttg aagtattcc 
      gtttataat cttagcggg aagtcgtat cattcgaag 
      tttgggcaa gcgagggtt gtcgattgt acgtttgcg 
      tatcctgaa cattttaaa gctccgttg ttgaattcg 
      ctgtaccag ctttgcgag gatatttat cagtatttg 
      gctggtgat agtcggaat gtggtcgtt gttcactgc 
      acggacgga aaagccacc tctgccacc ctcgtatgc 
      ggcttactt atttattca tttctttac gaagttcct 
      gaagatgcc ctacaaatg tttgccgtc aagcgctgt 
      ccacccaat atgagacct tccgaatta agatacctg 
      tactatttg gcagatatt gtgaaaaac ccgccggct 
      tatccgcac tataaaccg gtcactttt gtctcgatt 
      cagatgcag ccggtccca cttttcacc aaaattaga 
      gatgggtgt cgcccgtat ttggaggtt tacagcgag 
      aatcggtgc ttactttcg acgctacaa gactacgaa 
      aaaatgaga ttgtataat gttaccgaa ggaaaagtt 
      ttattgccg atcaatgtg agcgtttgt ggcgacgtt 
      tgcatcacc atttaccac gcaagaaac atcctaggg 
      ggcgtcatg acccaaggc aaggctaca gggattaaa 
      atctgccaa ctgcaatta cacaccggt tttataccg 
      gaggaggaa acctgtttg aaatttaac aaatcggaa 
      ttggatgaa atatccgac ggtcaggat cactaccag 
      gacagattt atggttctt ttaaacgtt tttgtgtcc 
      gattcggaa cgaaaaccc tcacagccg cccccatgg 
      ttgacggac aagacgcaa cgtgtttgt gatactatg 
      tttagttcg aagttagaa caagacgag acgatagat 
      aattttgcc agtaaacaa ccaaagcgg gtgcaagtg 
      tcaccccag aagccttca gagcgtccg aataggcct 
      gcgcccccg tcccccagg cccgcacac gtagaacta 
      gaacagaaa gattccagc gattccgag gccgagcaa 
      gtcaagcca acgcctaag ccagtggag cccccctta 
      atcgaagcc gtcgactta ctcaacctc aacaccacc 
      cccaacccc tcgaccata aagccctcc gcaagctca 
      aacttcgat ctcttaagc ggtttggat tcctccaac 
      gacaacttc ccctcattt ccctcgaat cccgctccc 
      aacccccag ccgtcgctc tttgacccg ttcggttcc 
      tcggacccc caaaataac ctcctcggc gattggggt 
      aatttcaat tcggcccct ccgactaag ccccccgag 
      ccccagccc cagaaaccg accgacttt ttctccgac 
      ttgggcaat ctgggcgga ggtgcgtgg aacagcaac 
      gcgtcctcg cccatcaaa gtccccccc acgaccccc 
      caacaaggg ggcaccccc caacacaag cccgtcacg 
      ccgcaacac caagccaag tcgccggat tacagcaga 
      tcccacttt gatgcgttc ggcgataag ggcgagaaa 
      gggaagccg aagagcgat gatgtcttt ggggatttg 
      ctggggtcg caggggtac aagttcagc gcgaagaaa 
      gacaacgcc cctaggacg atcaacgat atgaggaaa 
      gaggatatg gcgacttac atggaccct gataagatt 
      aaaatcatg gaatggaaa gagggcaag aagaacaat 
      atcagggcg ttgctgtgt tcgctacat acggtcctg 
      tgggaaggg acgaagtgg aataagtgc gatatgtcg 
      cagctggtg acacccgcc gatgttaag aaggcgtac 
      aggaaggcc tgtttggcg gtgcatccg gataagcaa 
      acgggtacc gataacgag aacatggcc aagctgatt 
      tttatggaa ttgaacaat gcctggagt gacttcgaa 
      aatgatgcc tcccaacaa aatatcttc actagctaa 
      ttcttctct actatgtga tcattccgt tttatggtt 
      aacttatag attatttat tttaggtga ttttatttc 
      aattttaca taacaatga tgtagctct aaagtgatg 
      actttatca tatgaaatg aaagcaatt gtcggatct 
      agacaatat tttaatcgt agttacttt agaggtgtt 
      gttaaaatt gagcgctga tgataatga tattatact 
      taatagttt tgttttgta ttgtttaga atatatttt 
      tatattgtg tcactgtta cataacgtc atttgtgca 
      attcatgat tacgattcg tttccagtg ttttaaatt 
      ttattcaag ttgcaaact tcaaaaata tatctcaaa 
      gagcaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY