Status
Please wait ...
Gene information
General information
TC012060 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 6884971..6930860
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC012060
    iBeetle screen iB_05160

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages partially shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs segment shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    Adult morphology
    adult complete pigmentation/tanning lighter - Number of animals showing the phenotype: 3


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC012060 RA
      MILRLSRGG PRRAAFYAI LLLFVAAVL FRICILRLT 
      ENDPAMVSP SKFVVGPDH KIYEYNRDM PLIFIGGVP 
      RSGTTLMRA MLDAHPDVR CGQETRVIP RLLQLRFHW 
      LKSEKESLR LEEAGITKS VLDSAIAAF TLEIIVKHG 
      EPAPRLCNK DPLTIKMGT YVVDLFPNA KFIFMVRDG 
      RATAHSIIT RKVTITGFD LTSYRQCLK KWNSAVEAM 
      NNQCKELGP NRCLKVPYE QLVLHPRKW MEKVLKFLD 
      IPWNESVLH HHEFINNGV SLSKVERSS DQVIKPVNL 
      EALTKWVGS IPDDVVKDM AEIAPMLSV LGYDPYANP 
      PNYGRPDDV VATNTKDIQ KHKGDWEKK AKEMFNNRW 
      EEGGGGDTA LDKEPSDNP NNTT
    CDS sequence
    • >TC012060 RA
      atgatcctc cggttgagc cgcggcggc ccacgccgg 
      gcagccttt tacgccatt ctcctgctg ttcgtagca 
      gcggtcctg ttccgcatt tgtatactt cggctcacg 
      gaaaatgat cctgccatg gtgtcgccg agcaagttc 
      gtggtgggt cccgaccac aaaatctac gaatacaac 
      agggacatg ccgctcatc ttcataggg ggagtgccc 
      cgctcgggc accactctc atgcgtgcc atgctggac 
      gcacatccc gacgtgaga tgtggccaa gaaacccgc 
      gtgattccg cgcctcctg cagctgcgc ttccactgg 
      ctcaagtcc gagaaggaa tctctgcgc cttgaagag 
      gccggaata acaaaatcg gtcctcgat tcggccatc 
      gccgctttc acgctcgaa ataatagta aaacacgga 
      gagccggct ccacgtctc tgcaacaaa gacccgctc 
      acaatcaaa atgggcact tacgtcgtg gacctcttc 
      cccaacgcc aagtttatt tttatggtg cgagacgga 
      cgtgccacg gcgcactcg atcatcacg cggaaagtc 
      accatcact ggcttcgat ttgaccagt taccggcaa 
      tgcctgaaa aagtggaac agtgccgtc gaagcgatg 
      aacaatcag tgcaaagaa ctggggccg aataggtgt 
      ctgaaggtg ccctacgaa cagttggtg ctgcatccc 
      aggaagtgg atggagaaa gtgctcaag ttcttggac 
      attccgtgg aacgagtcc gtgctgcac caccacgag 
      ttcatcaat aatggtgtc agcttgagc aaggtggag 
      cgttcctcc gaccaggtg ataaagccg gtcaatcta 
      gaagcttta acaaagtgg gtgggcagc atccccgac 
      gacgtggtc aaagacatg gccgagatt gcgcccatg 
      ttgtcagta ttaggctac gacccatac gctaatcct 
      ccgaactac ggcagaccc gacgacgtc gtcgcgacc 
      aacacgaaa gacatccaa aagcacaaa ggagactgg 
      gagaagaaa gccaaagaa atgttcaat aacaggtgg 
      gaggagggc ggcggtgga gatacggcc ctcgacaaa 
      gagcccagt gataaccca aacaatacc acgtga
    mRNA sequence
    • >TC012060 RA
      aaaatattt aacggattt aataaactg taatttttt 
      ccagatttc ggaaagaag cgtggaact ctggacttt 
      ttataagtt atgacaagt atgaggtga aacaatctc 
      gaggatgat cctccggtt gagccgcgg cggcccacg 
      ccgggcagc cttttacgc cattctcct gctgttcgt 
      agcagcggt cctgttccg catttgtat acttcggct 
      cacggaaaa tgatcctgc catggtgtc gccgagcaa 
      gttcgtggt gggtcccga ccacaaaat ctacgaata 
      caacaggga catgccgct catcttcat agggggagt 
      gccccgctc gggcaccac tctcatgcg tgccatgct 
      ggacgcaca tcccgacgt gagatgtgg ccaagaaac 
      ccgcgtgat tccgcgcct cctgcagct gcgcttcca 
      ctggctcaa gtccgagaa ggaatctct gcgccttga 
      agaggccgg aataacaaa atcggtcct cgattcggc 
      catcgccgc tttcacgct cgaaataat agtaaaaca 
      cggagagcc ggctccacg tctctgcaa caaagaccc 
      gctcacaat caaaatggg cacttacgt cgtggacct 
      cttccccaa cgccaagtt tatttttat ggtgcgaga 
      cggacgtgc cacggcgca ctcgatcat cacgcggaa 
      agtcaccat cactggctt cgatttgac cagttaccg 
      gcaatgcct gaaaaagtg gaacagtgc cgtcgaagc 
      gatgaacaa tcagtgcaa agaactggg gccgaatag 
      gtgtctgaa ggtgcccta cgaacagtt ggtgctgca 
      tcccaggaa gtggatgga gaaagtgct caagttctt 
      ggacattcc gtggaacga gtccgtgct gcaccacca 
      cgagttcat caataatgg tgtcagctt gagcaaggt 
      ggagcgttc ctccgacca ggtgataaa gccggtcaa 
      tctagaagc tttaacaaa gtgggtggg cagcatccc 
      cgacgacgt ggtcaaaga catggccga gattgcgcc 
      catgttgtc agtattagg ctacgaccc atacgctaa 
      tcctccgaa ctacggcag acccgacga cgtcgtcgc 
      gaccaacac gaaagacat ccaaaagca caaaggaga 
      ctgggagaa gaaagccaa agaaatgtt caataacag 
      gtgggagga gggcggcgg tggagatac ggccctcga 
      caaagagcc cagtgataa cccaaacaa taccacgtg 
      ataatacgt gtacacagt taggtgttt tcaattcga 
      ttgtgtgtg gaccaagac ggccgagtg acttattcg 
      tgtgagaag acggtgctt tttgatgta ttgtgacca 
      gccagtatc agaactcta tttaattta tttataatt 
      gtttgactt gtttgacac gttatttgg actaacgag 
      tctgttgat tggatttgt ttcattgca actaacttt 
      gttacattt tgtaaattt ttcccaaat ttgacttct 
      tgaataaat attttatta caat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY